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[PMID]:28499036
[Au] Autor:O'Donnell KL; Bixby MA; Morin KJ; Bradley DS; Vaughan JA
[Ad] Endereço:Department of Basic Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202.
[Ti] Título:Potential of a Northern Population of Aedes vexans (Diptera: Culicidae) to Transmit Zika Virus.
[So] Source:J Med Entomol;54(5):1354-1359, 2017 Sep 01.
[Is] ISSN:1938-2928
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Zika virus is an emerging arbovirus of humans in the western hemisphere. With its potential spread into new geographical areas, it is important to define the vector competence of native mosquito species. We tested the vector competency of Aedes vexans (Meigen) from the Lake Agassiz Plain of northwestern Minnesota and northeastern North Dakota. Aedes aegypti (L.) was used as a positive control for comparison. Mosquitoes were fed blood containing Zika virus and 2 wk later were tested for viral infection and dissemination. Aedes vexans (n = 60) were susceptible to midgut infection (28% infection rate) but displayed a fairly restrictive midgut escape barrier (3% dissemination rate). Cofed Ae. aegypti (n = 22) displayed significantly higher rates of midgut infection (61%) and dissemination (22%). To test virus transmission, mosquitoes were inoculated with virus and 16-17 d later, tested for their ability to transmit virus into fluid-filled capillary tubes. Unexpectedly, the transmission rate was significantly higher for Ae. vexans (34%, n = 47) than for Ae. aegypti (5%, n = 22). The overall transmission potential for Ae. vexans to transmit Zika virus was 1%. Because of its wide geographic distribution, often extreme abundance, and aggressive human biting activity, Ae. vexans could serve as a potential vector for Zika virus in northern latitudes where the conventional vectors, Ae. aegypti and Ae. albopictus Skuse, cannot survive. However, Zika virus is a primate virus and humans are the only amplifying host species in northern latitudes. To serve as a vector of Zika virus, Ae. vexans must feed repeatedly on humans. Defining the propensity of Ae. vexans to feed repeatedly on humans will be key to understanding its role as a potential vector of Zika virus.
[Mh] Termos MeSH primário: Aedes/virologia
Insetos Vetores/virologia
Infecção pelo Zika virus/transmissão
Zika virus/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Seres Humanos
Minnesota
North Dakota
Infecção pelo Zika virus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjx087


  2 / 1126 MEDLINE  
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[PMID]:28472369
[Au] Autor:Otto CRV; O'Dell S; Bryant RB; Euliss NH; Bush RM; Smart MD
[Ad] Endereço:U.S. Geological Survey Northern Prairie Wildlife Research Center, Jamestown, ND 58401 (cotto@usgs.gov; sam.odell@gmail.com; Russ.Bryant@humboldt.edu; eulissfamilyinnd@gmail.com; msmart@usgs.gov).
[Ti] Título:Using Publicly Available Data to Quantify Plant-Pollinator Interactions and Evaluate Conservation Seeding Mixes in the Northern Great Plains.
[So] Source:Environ Entomol;46(3):565-578, 2017 06 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Concern over declining pollinators has led to multiple conservation initiatives for improving forage for bees in agroecosystems. Using data available through the Pollinator Library (npwrc.usgs.gov/pollinator/), we summarize plant-pollinator interaction data collected from 2012-2015 on lands managed by the U.S. Fish and Wildlife Service and private lands enrolled in U.S. Department of Agriculture conservation programs in eastern North Dakota (ND). Furthermore, we demonstrate how plant-pollinator interaction data from the Pollinator Library and seed cost information can be used to evaluate hypothetical seeding mixes for pollinator habitat enhancements. We summarize records of 314 wild bee and 849 honey bee (Apis mellifera L.) interactions detected on 63 different plant species. The wild bee observations consisted of 46 species, 15 genera, and 5 families. Over 54% of all wild bee observations were represented by three genera-Bombus, Lassioglossum, and Melissodes. The most commonly visited forbs by wild bees were Monarda fistulosa, Sonchus arvensis, and Zizia aurea. The most commonly visited forbs by A. mellifera were Cirsium arvense, Melilotus officinalis, and Medicago sativa. Among all interactions, 13% of A. mellifera and 77% of wild bee observations were made on plants native to ND. Our seed mix evaluation shows that mixes may often need to be tailored to meet the unique needs of wild bees and managed honey bees in agricultural landscapes. Our evaluation also demonstrates the importance of incorporating both biologic and economic information when attempting to design cost-effective seeding mixes for supporting pollinators in a critically important part of the United States.
[Mh] Termos MeSH primário: Agricultura/métodos
Abelhas/fisiologia
Conservação dos Recursos Naturais
Polinização
[Mh] Termos MeSH secundário: Animais
North Dakota
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx070


  3 / 1126 MEDLINE  
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[PMID]:28423428
[Au] Autor:Wenninger EJ; Emmert SY; Tindall K; Ding H; Boetel MA; Rajabaskar D; Eigenbrode SD
[Ad] Endereço:Department of Plant, Soil, and Entomological Sciences, University of Idaho, Kimberly Research & Extension Center, Kimberly, ID 83341.
[Ti] Título:Aggregation Behavior and a Putative Aggregation Pheromone in Sugar Beet Root Maggot Flies (Diptera: Ulidiidae).
[So] Source:J Insect Sci;17(1), 2017 Jan 01.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Male-biased aggregations of sugar beet root maggot, Tetanops myopaeformis (Röder) (Diptera: Ulidiidae), flies were observed on utility poles near sugar beet (Beta vulgaris L. [Chenopodiaceae]) fields in southern Idaho; this contrasts with the approximately equal sex ratio typically observed within fields. Peak observation of mating pairs coincided with peak diurnal abundance of flies. Volatiles released by individual male and female flies were sampled from 08:00 to 24:00 hours in the laboratory using solid-phase microextraction and analyzed using gas chromatography/mass spectrometry (GC/MS). Eleven compounds were uniquely detected from males. Three of these compounds (2-undecanol, 2-decanol, and sec-nonyl acetate) were detected in greater quantities during 12:00-24:00 hours than during 08:00-12:00 hours. The remaining eight compounds uniquely detected from males did not exhibit temporal trends in release. Both sexes produced 2-nonanol, but males produced substantially higher (ca. 80-fold) concentrations of this compound than females, again peaking after 12:00 hours. The temporal synchrony among male aggregation behavior, peak mating rates, and release of certain volatile compounds by males suggest that T. myopaeformis flies exhibit lekking behavior and produce an associated pheromone. Field assays using synthetic blends of the putative aggregation pheromone showed evidence of attraction in both females and males.
[Mh] Termos MeSH primário: Quimiotaxia/efeitos dos fármacos
Dípteros/fisiologia
Feromônios/farmacologia
Atrativos Sexuais/farmacologia
Comportamento Sexual Animal
[Mh] Termos MeSH secundário: Animais
Beta vulgaris/crescimento & desenvolvimento
Feminino
Idaho
Masculino
North Dakota
Feromônios/metabolismo
Atrativos Sexuais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones); 0 (Sex Attractants); 0 (insect attractants)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1093/jisesa/iew123


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[PMID]:28401762
[Au] Autor:Gvakharia A; Kort EA; Brandt A; Peischl J; Ryerson TB; Schwarz JP; Smith ML; Sweeney C
[Ad] Endereço:Climate and Space Sciences and Engineering, University of Michigan , Ann Arbor, Michigan 48109, United States.
[Ti] Título:Methane, Black Carbon, and Ethane Emissions from Natural Gas Flares in the Bakken Shale, North Dakota.
[So] Source:Environ Sci Technol;51(9):5317-5325, 2017 May 02.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Incomplete combustion during flaring can lead to production of black carbon (BC) and loss of methane and other pollutants to the atmosphere, impacting climate and air quality. However, few studies have measured flare efficiency in a real-world setting. We use airborne data of plume samples from 37 unique flares in the Bakken region of North Dakota in May 2014 to calculate emission factors for BC, methane, ethane, and combustion efficiency for methane and ethane. We find no clear relationship between emission factors and aircraft-level wind speed or between methane and BC emission factors. Observed median combustion efficiencies for methane and ethane are close to expected values for typical flares according to the US EPA (98%). However, we find that the efficiency distribution is skewed, exhibiting log-normal behavior. This suggests incomplete combustion from flares contributes almost 1/5 of the total field emissions of methane and ethane measured in the Bakken shale, more than double the expected value if 98% efficiency was representative. BC emission factors also have a skewed distribution, but we find lower emission values than previous studies. The direct observation for the first time of a heavy-tail emissions distribution from flares suggests the need to consider skewed distributions when assessing flare impacts globally.
[Mh] Termos MeSH primário: Metano
Gás Natural
[Mh] Termos MeSH secundário: Poluentes Atmosféricos
Carbono
Etano
North Dakota
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Natural Gas); 7440-44-0 (Carbon); L99N5N533T (Ethane); OP0UW79H66 (Methane)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.6b05183


  5 / 1126 MEDLINE  
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[PMID]:28254199
[Au] Autor:Jahns L; Conrad Z; Johnson LK; Scheett AJ; Stote KS; Raatz SK
[Ti] Título:Diet Quality Is Lower and Energy Intake Is Higher on Weekends Compared with Weekdays in Midlife Women: A 1-Year Cohort Study.
[So] Source:J Acad Nutr Diet;117(7):1080-1086.e1, 2017 Jul.
[Is] ISSN:2212-2672
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Differences in energy and macronutrient intakes by weekday and weekend have been reported, but there are few data on differences in food group consumption and indices of diet quality. OBJECTIVE: The aim of this study was to describe dietary intake by day and on weekends compared with weekdays. DESIGN: This study utilized a longitudinal cohort design with self-reported dietary intake collected using the Automated Self-Administered 24-Hour Dietary Assessment Tool. PARTICIPANTS/SETTING: Participants were ambulatory women with access to high-speed internet, body mass index between 18 and 35, and aged 40 to 60 years from the Life in All Seasons study (n=52) conducted in Grand Forks, ND, between July 2012 and July 2014. Each woman completed an Automated Self-Administered 24-Hour Dietary Assessment Tool diet recall every 10 days for 1 year. MAIN OUTCOME MEASURES: Primary outcome measures were total and energy intake from macronutrients, food groups (per 1,000 kcal), and Healthy Eating Index-2010 (HEI-2010) scores from 1,866 24-hour recalls. STATISTICAL ANALYSES PERFORMED: Effects of weekend on energy and macronutrient intake, energy-adjusted food groups, and HEI-2010 scores and component scores were tested using mixed linear models. RESULTS: Participants (n=52) completed 1,080 recalls on weekdays and 786 on weekends. Seventy-five percent of women reported consuming more energy on weekends than on weekdays, with a higher mean intake of 158 kcal on weekends (P<0.01). The percentage of energy from carbohydrate (P<0.01) and protein (P<0.01) were both lower on weekends, and percentage of energy from alcohol higher (P<0.01). There was no difference in the percentage of energy from fat (P=0.07). Reported energy intake was greatest on Saturdays and lowest on Tuesdays. On weekends, women had reduced diet quality with more alcoholic beverages, solid fat, and potatoes, and less yogurt, whole fruits, dark green and orange vegetables, poultry, nuts and seeds, and whole grains per 1,000 kcal than on weekdays. HEI-2010 scores were lower on weekends than weekdays (P<0.01). Component scores were lower for whole fruits (P<0.01), greens and beans (P=0.02), whole grains (P<0.01), and dairy (P<0.01). CONCLUSIONS: Midlife women should be encouraged to maintain diet quality during weekends to improve overall diet quality scores.
[Mh] Termos MeSH primário: Ingestão de Energia
Dieta Saudável
[Mh] Termos MeSH secundário: Adulto
Consumo de Bebidas Alcoólicas
Índice de Massa Corporal
Estudos de Coortes
Dieta
Registros de Dieta
Gorduras na Dieta/administração & dosagem
Comportamento Alimentar
Feminino
Seres Humanos
Atividades de Lazer
Estudos Longitudinais
Meia-Idade
North Dakota
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


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[PMID]:28187248
[Au] Autor:Mohr NM; Harland KK; Chrischilles EA; Bell A; Shane DM; Ward MM
[Ad] Endereço:Department of Emergency Medicine, University of Iowa Carver College of Medicine, Iowa City, IA.
[Ti] Título:Emergency Department Telemedicine Is Used for More Severely Injured Rural Trauma Patients, but Does Not Decrease Transfer: A Cohort Study.
[So] Source:Acad Emerg Med;24(2):177-185, 2017 Feb.
[Is] ISSN:1553-2712
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Traumatic injury is a leading cause of death in the United States, and rural populations are at increased risk of injury and death. Rural residents have limited access to trauma care, and telemedicine has been proposed as one strategy to improve the provision of trauma care locally. The objective of this study was to describe patient-level factors associated with telemedicine consultation in North Dakota critical-access hospital (CAH) emergency departments (EDs) and to measure the association between telemedicine consultation and interhospital transfer. METHODS: Observational cohort study of all adult (age ≥ 18 years) trauma patients treated in North Dakota CAH EDs with an active telemedicine subscription between 2008 and 2014. Trauma cases were identified from the North Dakota Trauma Registry, and telemedicine-enabled care was determined using a probabilistic linking algorithm with the call records of the predominant telemedicine network in North Dakota. Multivariable generalized estimating equations were used to identify factors associated with telemedicine consultation and to measure the association between telemedicine consultation and interhospital transfer, adjusting for patient, injury, and hospital factors. RESULTS: Of the 9,281 North Dakota trauma patients seen in CAHs, 2,837 were treated in an ED with an active telemedicine subscription. Telemedicine was consulted for 11% of all trauma patients in telemedicine-capable EDs. Factors associated with telemedicine consultation included higher Injury Severity Score, penetrating injuries, burns, hypotension, tachycardia, and ambulance transport. Adjusting for severity of illness, injury mechanism, and type of injury, telemedicine use was not associated with interhospital transfer (adjusted odds ratio = 1.28, 95% confidence interval = 0.94 to 1.75). CONCLUSION: Emergency department-based telemedicine consultation is requested for the most severely injured rural trauma patients, especially with those with penetrating trauma, burns, and abnormal presenting vital signs. Telemedicine consultation was not independently associated with increased probability of transfer. Future work should evaluate how telemedicine impacts the timeliness of care and specific care interventions.
[Mh] Termos MeSH primário: Serviço Hospitalar de Emergência/estatística & dados numéricos
Escala de Gravidade do Ferimento
Transferência de Pacientes/estatística & dados numéricos
População Rural/estatística & dados numéricos
Telemedicina/utilização
Ferimentos e Lesões/terapia
[Mh] Termos MeSH secundário: Adulto
Estudos de Coortes
Feminino
Seres Humanos
Masculino
Meia-Idade
North Dakota
Razão de Chances
Sistema de Registros
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1111/acem.13120


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[PMID]:28096744
[Au] Autor:Bakker CJ; Koffel JB; Theis-Mahon NR
[Ti] Título:Measuring the health literacy of the Upper Midwest.
[So] Source:J Med Libr Assoc;105(1):34-43, 2017 Jan.
[Is] ISSN:1558-9439
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Health literacy-the ability to obtain, process, and understand basic health information-is a major determinant of an individual's overall health and health care utilization. In this project, the authors examined predictors of health literacy levels, including numeracy and graphic literacy, among an adult population in the Upper Midwest. METHODS: The research was conducted at the Minnesota State Fair. Three previously validated scales were used to assess health literacy: Newest Vital Sign, the General Health Numeracy Test, and questions from Galesic and Garcia-Retamero's Graph Literacy Scale. Demographic information-such as age, educational attainment, zip code, and other potential predictors and modifiers-was collected. Multivariate linear regression was conducted to examine the independent effects of educational attainment, race, ethnicity, gender, and rural or urban location on overall health literacy and scores on each of the individual instruments. RESULTS: A total of 353 Upper Midwest residents completed the survey, with the majority being white, college-educated, and from an urban area. Having a graduate or professional degree or being under the age of 21 were associated with increased health literacy scores, while having a high school diploma or some high school education, being Asian American, or being American Indian/Alaska Native were associated with lower health literacy scores. CONCLUSION: Advanced health literacy skills, including the ability to calculate and compare information, were problematic even in well-educated populations. Understanding numerical and graphical information was found to be particularly difficult, and more research is needed to understand these deficits and how best to address them.
[Mh] Termos MeSH primário: Alfabetização em Saúde/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Fatores Etários
Idoso
Escolaridade
Feminino
Seres Humanos
Iowa
Masculino
Meia-Idade
Minnesota
North Dakota
Inquéritos e Questionários
Wisconsin
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.5195/jmla.2017.105


  8 / 1126 MEDLINE  
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[PMID]:28081245
[Au] Autor:Burr PC; Robinson AC; Larsen RT; Newman RA; Ellis-Felege SN
[Ad] Endereço:Department of Biology, University of North Dakota, Grand Forks, ND, United States of America.
[Ti] Título:Sharp-Tailed Grouse Nest Survival and Nest Predator Habitat Use in North Dakota's Bakken Oil Field.
[So] Source:PLoS One;12(1):e0170177, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent advancements in extraction technologies have resulted in rapid increases of gas and oil development across the United States and specifically in western North Dakota. This expansion of energy development has unknown influences on local wildlife populations and the ecological interactions within and among species. Our objectives for this study were to evaluate nest success and nest predator dynamics of sharp-tailed grouse (Tympanuchus phasianellus) in two study sites that represented areas of high and low energy development intensities in North Dakota. During the summers of 2012 and 2013, we monitored 163 grouse nests using radio telemetry. Of these, 90 nests also were monitored using miniature cameras to accurately determine nest fates and identify nest predators. We simultaneously conducted predator surveys using camera scent stations and occupancy modeling to estimate nest predator occurrence at each site. American badgers (Taxidea taxus) and striped skunks (Mephitis mephitis) were the primary nest predators, accounting for 56.7% of all video recorded nest depredations. Nests in our high intensity gas and oil area were 1.95 times more likely to succeed compared to our minimal intensity area. Camera monitored nests were 2.03 times more likely to succeed than non-camera monitored nests. Occupancy of mammalian nest predators was 6.9 times more likely in our study area of minimal gas and oil intensity compared to the high intensity area. Although only a correlative study, our results suggest energy development may alter the predator community, thereby increasing nest success for sharp-tailed grouse in areas of intense development, while adjacent areas may have increased predator occurrence and reduced nest success. Our study illustrates the potential influences of energy development on the nest predator-prey dynamics of sharp-tailed grouse in western North Dakota and the complexity of evaluating such impacts on wildlife.
[Mh] Termos MeSH primário: Ecossistema
Galliformes/fisiologia
Comportamento de Nidação/fisiologia
Campos de Petróleo e Gás
[Mh] Termos MeSH secundário: Animais
North Dakota
Razão de Chances
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170177


  9 / 1126 MEDLINE  
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[PMID]:28076921
[Au] Autor:Gold A; Larson M; Tucker J; Strang M
[Ad] Endereço:Department of Public Health, North Dakota State University, PO Box 6050-Dept. 2662, Fargo, ND 58108-6050.
[Ti] Título:Classroom Nutrition Education Combined With Fruit and Vegetable Taste Testing Improves Children's Dietary Intake.
[So] Source:J Sch Health;87(2):106-113, 2017 Feb.
[Is] ISSN:1746-1561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We tested the effectiveness of the Go Wild With Fruits and Veggies! (GWWFV) Extension curriculum on increasing fruit and vegetable (FV) intake of third graders. METHODS: An intervention study was used testing self-reported FV intake pre/post GWWFV. Recruited schools were randomized to control (12 schools, N = 369, third grade children) or intervention (14 schools, N = 378, third grade children). Measures included items from a validated FV food frequency survey. Access to the federal Fresh Fruit and Vegetable Snack Program (FFVSP), and taste testing within GWWFV were also measured. Between-group differences in FV intake were evaluated using 1-way ANOVA for unadjusted models and 1-way ANCOVA for baseline adjusted models. The Tukey-Kramer test was employed for post hoc comparisons. RESULTS: Students with access to the FFVSP consumed more fruits (p < .01) and vegetables (p < .0001). Students who participated in the FFVSP and GWWFV with taste testing consumed more fruits and vegetables (p < .05) compared with students who participated in the FFVSP and GWWFV without taste testing, along with the control group. CONCLUSIONS: The FFVSP and GWWFV with taste testing demonstrated a positive influence on FV intake in third grade children. Leveraging environmental approaches with interactive learning experiences create desired outcomes.
[Mh] Termos MeSH primário: Dieta
Frutas
Educação em Saúde
Instituições Acadêmicas
Paladar
Verduras
[Mh] Termos MeSH secundário: Feminino
Promoção da Saúde
Seres Humanos
Masculino
North Dakota
Obesidade/prevenção & controle
Autorrelato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM; N
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1111/josh.12478


  10 / 1126 MEDLINE  
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[PMID]:28062536
[Au] Autor:Smart MD; Cornman RS; Iwanowicz DD; McDermott-Kubeczko M; Pettis JS; Spivak MS; Otto CR
[Ad] Endereço:U.S. Geological Survey Northern Prairie Wildlife Research Center, Jamestown, ND (msmart@usgs.gov; cotto@usgs.gov).
[Ti] Título:A Comparison of Honey Bee-Collected Pollen From Working Agricultural Lands Using Light Microscopy and ITS Metabarcoding.
[So] Source:Environ Entomol;46(1):38-49, 2017 02 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been tested on a spatially or temporally robust number of pollen samples. Here, we compare pollen identification results derived from light microscopy and DNA sequencing techniques with samples collected from honey bee colonies embedded within a gradient of intensive agricultural landscapes in the Northern Great Plains throughout the 2010-2011 growing seasons. We demonstrate that at all taxonomic levels, DNA sequencing was able to discern a greater number of taxa, and was particularly useful for the identification of infrequently detected species. Importantly, substantial phenological overlap did occur for commonly detected taxa using either technique, suggesting that DNA sequencing is an appropriate, and enhancing, substitutive technique for accurately capturing the breadth of bee-collected species of pollen present across agricultural landscapes. We also show that honey bees located in high and low intensity agricultural settings forage on dissimilar plants, though with overlap of the most abundantly collected pollen taxa. We highlight practical applications of utilizing sequencing technology, including addressing ecological issues surrounding land use, climate change, importance of taxa relative to abundance, and evaluating the impact of conservation program habitat enhancement efforts.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Código de Barras de DNA Taxonômico
Magnoliopsida/classificação
Microscopia
Pólen/classificação
[Mh] Termos MeSH secundário: Animais
Fazendas
Espécies Introduzidas
North Dakota
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvw159



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