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  1 / 5100 MEDLINE  
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[PMID]:29256430
[Au] Autor:Stimmelmayr R; Rotstein D; Seguel M; Gottdenker N
[Ad] Endereço:North Slope Borough Department of Wildlife Management, Barrow, Alaska 99723, USA.
[Ti] Título:Hepatic lipomas and myelolipomas in subsistence-harvested bowhead whales Balaena mysticetus, Alaska (USA): a case review 1980-2016.
[So] Source:Dis Aquat Organ;127(1):71-74, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We describe a case series of benign hepatic fatty tumors in 10 subsistence-harvested bowhead whales. Microscopic features included lipomatous and myelolipomatous masses. Extensive atrophy and/or destruction of hepatic parenchyma was not observed. No other significant disease was present except in an animal with unrelated chronic pleuritis. Based on our longitudinal case series (1980-2016) which identified 1-2 hepatic lipomas and myelolipomas in landed whales annually at Barrow, Alaska (USA), since 2012, hepatic lipomas and myelolipomas are occasionally seen in hunter-harvested bowhead whales. A conservative estimate for the percentage of bowhead whales with hepatic fatty tumors in landed whales in Barrow from 2012 to 2016 was 6% (7/111). The pathogenesis and exact cell origin of these benign fatty tumors in bowhead whales is undetermined. Assessment of further cases is warranted to better define the tissue distribution and pathogenesis of these tumors in bowhead whale liver.
[Mh] Termos MeSH primário: Lipoma/veterinária
Neoplasias Hepáticas/veterinária
Mielolipoma/veterinária
Baleias
[Mh] Termos MeSH secundário: Alaska
Animais
Feminino
Lipoma/epidemiologia
Lipoma/patologia
Neoplasias Hepáticas/epidemiologia
Masculino
Mielolipoma/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03186


  2 / 5100 MEDLINE  
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[PMID]:29407642
[Au] Autor:Murphy DM; Froyd KD; Apel E; Blake D; Blake N; Evangeliou N; Hornbrook RS; Peischl J; Ray E; Ryerson TB; Thompson C; Stohl A
[Ad] Endereço:NOAA ESRL Chemical Sciences Division, Boulder, CO, USA. Electronic address: daniel.m.murphy@noaa.gov.
[Ti] Título:An aerosol particle containing enriched uranium encountered in the remote upper troposphere.
[So] Source:J Environ Radioact;184-185:95-100, 2018 Apr.
[Is] ISSN:1879-1700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We describe a submicron aerosol particle sampled at an altitude of 7 km near the Aleutian Islands that contained a small percentage of enriched uranium oxide. U was 3.1 ±â€¯0.5% of U. During twenty years of aircraft sampling of millions of particles in the global atmosphere, we have rarely encountered a particle with a similarly high content of U and never a particle with enriched U. The bulk of the particle consisted of material consistent with combustion of heavy fuel oil. Analysis of wind trajectories and particle dispersion model results show that the particle could have originated from a variety of areas across Asia. The source of such a particle is unclear, and the particle is described here in case it indicates a novel source where enriched uranium was dispersed.
[Mh] Termos MeSH primário: Aerossóis/análise
Poluentes Radioativos do Ar/análise
Monitoramento de Radiação
Urânio/análise
[Mh] Termos MeSH secundário: Alaska
Atmosfera/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Air Pollutants, Radioactive); 4OC371KSTK (Uranium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  3 / 5100 MEDLINE  
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[PMID]:29320572
[Au] Autor:McCloskey SE; Uher-Koch BD; Schmutz JA; Fondell TF
[Ad] Endereço:U.S. Geological Survey, Alaska Science Center, Anchorage, Alaska, United States of America.
[Ti] Título:International migration patterns of Red-throated Loons (Gavia stellata) from four breeding populations in Alaska.
[So] Source:PLoS One;13(1):e0189954, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Identifying post-breeding migration and wintering distributions of migratory birds is important for understanding factors that may drive population dynamics. Red-throated Loons (Gavia stellata) are widely distributed across Alaska and currently have varying population trends, including some populations with recent periods of decline. To investigate population differentiation and the location of migration pathways and wintering areas, which may inform population trend patterns, we used satellite transmitters (n = 32) to describe migration patterns of four geographically separate breeding populations of Red-throated Loons in Alaska. On average (± SD) Red-throated Loons underwent long (6,288 ± 1,825 km) fall and spring migrations predominantly along coastlines. The most northern population (Arctic Coastal Plain) migrated westward to East Asia and traveled approximately 2,000 km farther to wintering sites than the three more southerly populations (Seward Peninsula, Yukon-Kuskokwim Delta, and Copper River Delta) which migrated south along the Pacific coast of North America. These migration paths are consistent with the hypothesis that Red-throated Loons from the Arctic Coastal Plain are exposed to contaminants in East Asia. The three more southerly breeding populations demonstrated a chain migration pattern in which the more northerly breeding populations generally wintered in more northerly latitudes. Collectively, the migration paths observed in this study demonstrate that some geographically distinct breeding populations overlap in wintering distribution while others use highly different wintering areas. Red-throated Loon population trends in Alaska may therefore be driven by a wide range of effects throughout the annual cycle.
[Mh] Termos MeSH primário: Migração Animal
Aves/fisiologia
[Mh] Termos MeSH secundário: Alaska
Animais
América do Norte
Dinâmica Populacional
Estações do Ano
Telemetria
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189954


  4 / 5100 MEDLINE  
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[PMID]:29211808
[Au] Autor:Kuhn CE; Chumbley K; Fritz L; Johnson D
[Ad] Endereço:Marine Mammal Laboratory, Alaska Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, Seattle, Washington, United States of America.
[Ti] Título:Estimating dispersal rates of Steller sea lion (Eumetopias jubatus) mother-pup pairs from a natal rookery using mark-resight data.
[So] Source:PLoS One;12(12):e0189061, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To monitor population trends of Steller sea lions (Eumetopias jubatus) in Alaska, newborn pups are counted during aerial surveys. These surveys are scheduled to occur after the majority of pups are born, but before pups begin to spend significant time in the water. Some studies have reported dispersal of mother-pup pairs away from breeding beaches during the pupping season (July), which may influence survey results. Using a multistate mark-recapture model with state uncertainty, we estimated the amount of dispersal during the pupping season based on observations of permanently marked sea lions. Research was conducted at land-based observation sites on Marmot Island, Alaska, between 2000 and 2013. Both marked adult females with dependent pups and marked pups were observed at two rookery beaches from May to July. Cumulative dispersal rates were minimal (< 1%) prior to the planned start of the aerial survey (23 June) and increased to 11.2% by the planned survey completion date (10 July). The increased cumulative dispersal rate during the remainder of the observation period (end of July) suggests potential bias in surveys that occur beyond 10 July, however surveys past this date are rare (< 10% between 1973 and 2016). As a result, movements of mother-pup pairs during the pupping season are not likely to influence aerial survey estimates.
[Mh] Termos MeSH primário: Leões-Marinhos/fisiologia
[Mh] Termos MeSH secundário: Alaska
Animais
Animais Recém-Nascidos
Feminino
Modelos Teóricos
Dinâmica Populacional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189061


  5 / 5100 MEDLINE  
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[PMID]:28916510
[Au] Autor:Dalouk K; Gandhi N; Jessel P; MacMurdy K; Zarraga IG; Lasarev M; Raitt M
[Ad] Endereço:From the Knight Cardiovascular Institute (K.D., N.G., P.J., K.M., I.G.Z., M.R.), Biostatistics and Design Program (M.L.), Oregon Clinical & Translational Research Institute (OCRTI), Oregon Health and Science University, Portland; and Electrophysiology Department, Portland Veterans Affairs Medica
[Ti] Título:Outcomes of Telemedicine Video-Conferencing Clinic Versus In-Person Clinic Follow-Up for Implantable Cardioverter-Defibrillator Recipients.
[So] Source:Circ Arrhythm Electrophysiol;10(9), 2017 Sep.
[Is] ISSN:1941-3084
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Implantable cardioverter-defibrillator (ICD) recipients require close follow-up that can be difficult for patients who have to travel long distances for clinic follow-up. We aimed to compare clinical outcomes between ICD patients followed-up in a telemedicine video-conferencing clinic (TMVC) and a conventional in-person clinic (CIC). We hypothesized that outcomes of patients followed in the TMVC are noninferior to the CIC. METHODS AND RESULTS: This retrospective study compares time to first appropriate ICD therapy, time to first inappropriate ICD therapy, time to first shock, and overall survival in patients followed in TMVC compared with CIC between 2001 and 2016. Two hundred and eighty-seven patients were followed in the TMVC group and 236 patients in the CIC. The average age of the TMVC and CIC groups was 64.13±9.38 and 65.23±8.57 years, respectively ( =0.164). There was no difference in the modified Seattle heart failure model score between the 2 groups (-0.12±1.0 versus -0.21±0.99; =0.287). The Charlson comorbidity index score was higher in the CIC group compared with the TMVC group (7.0 versus 6.0; =0.01). Mean duration of follow-up was 4.8 years. Adjusted and unadjusted tests of noninferiority found TMVC was not inferior to in-person follow-up for the prespecified outcomes. CONCLUSIONS: Video-conferencing ICD follow-up for patients in areas where electrophysiology subspecialty care is not available leads to outcomes that are noninferior to CIC follow-up.
[Mh] Termos MeSH primário: Desfibriladores Implantáveis
Telemedicina
Videoconferência
[Mh] Termos MeSH secundário: Idoso
Alaska
Feminino
Seguimentos
Seres Humanos
Masculino
Meia-Idade
Oregon
Estudos Retrospectivos
Taxa de Sobrevida
Resultado do Tratamento
Washington
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170917
[St] Status:MEDLINE


  6 / 5100 MEDLINE  
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[PMID]:28837606
[Au] Autor:Spivey TJ; Lindberg MS; Meixell BW; Smith KR; Puryear WB; Davis KR; Runstadler JA; Stallknecht DE; Ramey AM
[Ad] Endereço:Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, Alaska, United States of America.
[Ti] Título:Maintenance of influenza A viruses and antibody response in mallards (Anas platyrhynchos) sampled during the non-breeding season in Alaska.
[So] Source:PLoS One;12(8):e0183505, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prevalence of influenza A virus (IAV) infections in northern-breeding waterfowl has previously been reported to reach an annual peak during late summer or autumn; however, little is known about IAV infection dynamics in waterfowl populations persisting at high-latitude regions such as Alaska, during winter. We captured mallards (Anas platyrhynchos) throughout the non-breeding season (August-April) of 2012-2015 in Fairbanks and Anchorage, the two largest cities in Alaska, to assess patterns of IAV infection and antibody production using molecular methods and a standard serologic assay. In addition, we used virus isolation, genetic sequencing, and a virus microneutralization assay to characterize viral subtypes and to evaluate the immune response of mallards captured on multiple occasions through time. We captured 923 mallards during three successive sampling years: Fairbanks in 2012/13 and 2013/14, and Anchorage in 2014/15. Prevalence varied by age, season, and year/site with high and relatively stable estimates throughout the non-breeding season. Infected birds were detected in all locations/seasons except early-winter in Fairbanks during 2013/14. IAVs with 17 combinations of hemagglutinin (H1-5, H7-9, H11, H12) and neuraminidase (N1-6, N8, N9) subtypes were isolated. Antibodies to IAVs were detected throughout autumn and winter for all sampling locations and years, however, seroprevalence was higher among adults and varied among years. Mallards exhibited individual heterogeneity with regard to immune response, providing instances of both seroconversion and seroreversion to detected viral subtypes. The probability that an individual transitioned from one serostatus to another varied by age, with juvenile mallards having higher rates of seroconversion and seroreversion than adults. Our study provides evidence that a diversity of IAVs circulate in populations of mallards wintering at urban locations in Alaska, and we suggest waterfowl wintering at high-latitudes may play an important role in maintenance of viruses across breeding seasons.
[Mh] Termos MeSH primário: Anticorpos Antivirais/biossíntese
Patos/virologia
Vírus da Influenza A/isolamento & purificação
[Mh] Termos MeSH secundário: Alaska
Animais
Cruzamento
Patos/fisiologia
Vírus da Influenza A/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183505


  7 / 5100 MEDLINE  
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[PMID]:28821183
[Au] Autor:Peichel CL; Sullivan ST; Liachko I; White MA
[Ad] Endereço:Divisons of Basic Sciences and Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA
[Ti] Título:Improvement of the Threespine Stickleback Genome Using a Hi-C-Based Proximity-Guided Assembly.
[So] Source:J Hered;108(6):693-700, 2017 09 01.
[Is] ISSN:1465-7333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Scaffolding genomes into complete chromosome assemblies remains challenging even with the rapidly increasing sequence coverage generated by current next-generation sequence technologies. Even with scaffolding information, many genome assemblies remain incomplete. The genome of the threespine stickleback (Gasterosteus aculeatus), a fish model system in evolutionary genetics and genomics, is not completely assembled despite scaffolding with high-density linkage maps. Here, we first test the ability of a Hi-C based proximity-guided assembly (PGA) to perform a de novo genome assembly from relatively short contigs. Using Hi-C based PGA, we generated complete chromosome assemblies from a distribution of short contigs (20-100 kb). We found that 96.40% of contigs were correctly assigned to linkage groups (LGs), with ordering nearly identical to the previous genome assembly. Using available bacterial artificial chromosome (BAC) end sequences, we provide evidence that some of the few discrepancies between the Hi-C assembly and the existing assembly are due to structural variation between the populations used for the 2 assemblies or errors in the existing assembly. This Hi-C assembly also allowed us to improve the existing assembly, assigning over 60% (13.35 Mb) of the previously unassigned (~21.7 Mb) contigs to LGs. Together, our results highlight the potential of the Hi-C based PGA method to be used in combination with short read data to perform relatively inexpensive de novo genome assemblies. This approach will be particularly useful in organisms in which it is difficult to perform linkage mapping or to obtain high molecular weight DNA required for other scaffolding methods.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Smegmamorpha/genética
[Mh] Termos MeSH secundário: Alaska
Animais
Cromossomos Artificiais Bacterianos
Mapeamento de Sequências Contíguas
Genômica
Masculino
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.1093/jhered/esx058


  8 / 5100 MEDLINE  
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[PMID]:28801345
[Au] Autor:Scally SW; Law SC; Ting YT; Heemst JV; Sokolove J; Deutsch AJ; Bridie Clemens E; Moustakas AK; Papadopoulos GK; van der Woude D; Smolik I; Hitchon CA; Robinson DB; Ferucci ED; Bernstein CN; Meng X; Anaparti V; Huizinga T; Kedzierska K; Reid HH; Raychaudhuri S; Toes RE; Rossjohn J; El-Gabalawy H; Thomas R
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Infection and Immunity Program, Biomedicine Discovery Institute Monash University, Clayton, Australia.
[Ti] Título:Molecular basis for increased susceptibility of Indigenous North Americans to seropositive rheumatoid arthritis.
[So] Source:Ann Rheum Dis;76(11):1915-1923, 2017 11.
[Is] ISSN:1468-2060
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The pathogenetic mechanisms by which alleles are associated with anticitrullinated peptide antibody (ACPA)-positive rheumatoid arthritis (RA) are incompletely understood. RA high-risk alleles are known to share a common motif, the 'shared susceptibility epitope (SE)'. Here, the electropositive P4 pocket of HLA-DRB1 accommodates self-peptide residues containing citrulline but not arginine. HLA-DRB1 His/Phe13ß stratifies with ACPA-positive RA, while His13ßSer polymorphisms stratify with ACPA-negative RA and RA protection. Indigenous North American (INA) populations have high risk of early-onset ACPA-positive RA, whereby HLA-DRB1*04:04 and HLA-DRB1*14:02 are implicated as risk factors for RA in INA. However, HLA-DRB1*14:02 has a His13ßSer polymorphism. Therefore, we aimed to verify this association and determine its molecular mechanism. METHODS: HLA genotype was compared in 344 INA patients with RA and 352 controls. Structures of HLA-DRB1*1402-class II loaded with vimentin-64Arg , vimentin-64Cit and fibrinogen ß-74Cit were solved using X-ray crystallography. Vimentin-64Cit -specific and vimentin -specific CD4+ T cells were characterised by flow cytometry using peptide-histocompatibility leukocyte antigen (pHLA) tetramers. After sorting of antigen-specific T cells, TCRα and ß-chains were analysed using multiplex, nested PCR and sequencing. RESULTS: ACPA RA in INA was independently associated with . Consequent to the His13ßSer polymorphism and altered P4 pocket of HLA-DRB1*14:02, both citrulline and arginine were accommodated in opposite orientations. Oligoclonal autoreactive CD4+ effector T cells reactive with both citrulline and arginine forms of vimentin were observed in patients with HLA-DRB1*14:02 RA and at-risk ACPA first-degree relatives. HLA-DRB1*14:02-vimentin -specific and HLA-DRB1*14:02-vimentin-64Cit -specific CD4+ memory T cells were phenotypically distinct populations. CONCLUSION: HLA-DRB1*14:02 broadens the capacity for citrullinated and native self-peptide presentation and T cell expansion, increasing risk of ACPA+ RA.
[Mh] Termos MeSH primário: Nativos do Alasca/genética
Artrite Reumatoide/etnologia
Artrite Reumatoide/genética
Predisposição Genética para Doença/etnologia
Cadeias HLA-DRB1/genética
Índios Norte-Americanos/genética
[Mh] Termos MeSH secundário: Alaska/etnologia
Alelos
Arginina/genética
Arginina/imunologia
Autoanticorpos/sangue
Autoanticorpos/imunologia
Linfócitos T CD4-Positivos/imunologia
Canadá/etnologia
Estudos de Casos e Controles
Citrulina/genética
Citrulina/imunologia
Feminino
Citometria de Fluxo
Genótipo
Seres Humanos
Masculino
Peptídeos Cíclicos/imunologia
Polimorfismo Genético
Fatores de Risco
Vimentina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantibodies); 0 (HLA-DRB1 Chains); 0 (Peptides, Cyclic); 0 (Vimentin); 0 (cyclic citrullinated peptide); 29VT07BGDA (Citrulline); 94ZLA3W45F (Arginine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171114
[Lr] Data última revisão:
171114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE
[do] DOI:10.1136/annrheumdis-2017-211300


  9 / 5100 MEDLINE  
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[PMID]:28750094
[Au] Autor:Rodríguez MM; Herman R; Ghiglione B; Kerff F; D'Amico González G; Bouillenne F; Galleni M; Handelsman J; Charlier P; Gutkind G; Sauvage E; Power P
[Ad] Endereço:Cátedra de Microbiología, Departamento de Microbiología, Inmunología y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
[Ti] Título:Crystal structure and kinetic analysis of the class B3 di-zinc metallo-ß-lactamase LRA-12 from an Alaskan soil metagenome.
[So] Source:PLoS One;12(7):e0182043, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We analyzed the kinetic properties of the metagenomic class B3 ß-lactamase LRA-12, and determined its crystallographic structure in order to compare it with prevalent metallo-ß-lactamases (MBLs) associated with clinical pathogens. We showed that LRA-12 confers extended-spectrum resistance on E. coli when expressed from recombinant clones, and the MIC values for carbapenems were similar to those observed in enterobacteria expressing plasmid-borne MBLs such as VIM, IMP or NDM. This was in agreement with the strong carbapenemase activity displayed by LRA-12, similar to GOB ß-lactamases. Among the chelating agents evaluated, dipicolinic acid inhibited the enzyme more strongly than EDTA, which required pre-incubation with the enzyme to achieve measurable inhibition. Structurally, LRA-12 contains the conserved main structural features of di-zinc class B ß-lactamases, and presents unique structural signatures that differentiate this enzyme from others within the family: (i) two loops (α3-ß7 and ß11-α5) that could influence antibiotic entrance and remodeling of the active site cavity; (ii) a voluminous catalytic cavity probably responsible for the high hydrolytic efficiency of the enzyme; (iii) the absence of disulfide bridges; (iv) a unique Gln116 at metal-binding site 1; (v) a methionine residue at position 221that replaces Cys/Ser found in other B3 ß-lactamases in a predominantly hydrophobic environment, likely playing a role in protein stability. The structure of LRA-12 indicates that MBLs exist in wild microbial populations in extreme environments, or environments with low anthropic impact, and under the appropriate antibiotic selective pressure could be captured and disseminated to pathogens.
[Mh] Termos MeSH primário: Metagenoma
Solo
Zinco/metabolismo
beta-Lactamases/química
[Mh] Termos MeSH secundário: Alaska
Sequência de Aminoácidos
Proteínas de Bactérias/metabolismo
Biocatálise/efeitos dos fármacos
Domínio Catalítico
Quelantes/farmacologia
Cristalografia por Raios X
Farmacorresistência Bacteriana/efeitos dos fármacos
Ácido Edético/farmacologia
Escherichia coli/efeitos dos fármacos
Cinética
Testes de Sensibilidade Microbiana
Modelos Moleculares
Fenótipo
Análise de Sequência de Proteína
beta-Lactamases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Chelating Agents); 0 (Soil); 9G34HU7RV0 (Edetic Acid); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (carbapenemase); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182043


  10 / 5100 MEDLINE  
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[PMID]:28727811
[Au] Autor:Kasanke CP; Leigh MB
[Ad] Endereço:Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, United States of America.
[Ti] Título:Factors limiting sulfolane biodegradation in contaminated subarctic aquifer substrate.
[So] Source:PLoS One;12(7):e0181462, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sulfolane, a water-soluble organosulfur compound, is used industrially worldwide and is associated with one of the largest contaminated groundwater plumes in the state of Alaska. Despite being widely used, little is understood about the degradation of sulfolane in the environment, especially in cold regions. We conducted aerobic and anaerobic microcosm studies to assess the biological and abiotic sulfolane degradation potential of contaminated subarctic aquifer groundwater and sediment from Interior Alaska. We also investigated the impacts of nutrient limitations and hydrocarbon co-contamination on sulfolane degradation. We found that sulfolane underwent biodegradation aerobically but not anaerobically under nitrate, sulfate, or iron-reducing conditions. No abiotic degradation activity was detectable under either oxic or anoxic conditions. Nutrient addition stimulated sulfolane biodegradation in sediment slurries at high sulfolane concentrations (100 mg L-1), but not at low sulfolane concentrations (500 µg L-1), and nutrient amendments were necessary to stimulate sulfolane biodegradation in incubations containing groundwater only. Hydrocarbon co-contamination retarded aerobic sulfolane biodegradation rates by ~30%. Our study is the first to investigate the sulfolane biodegradation potential of subarctic aquifer substrate and identifies several important factors limiting biodegradation rates. We concluded that oxygen is an important factor limiting natural attenuation of this sulfolane plume, and that nutrient amendments are unlikely to accelerate biodegradation within in the plume, although they may biostimulate degradation in ex situ groundwater treatment applications. Future work should be directed at elucidating the identity of indigenous sulfolane-degrading microorganisms and determining their distribution and potential activity in the environment.
[Mh] Termos MeSH primário: Água Subterrânea/química
Tiofenos/química
Poluentes Químicos da Água/química
[Mh] Termos MeSH secundário: Alaska
Análise de Variância
Biodegradação Ambiental
Água Subterrânea/microbiologia
Hidrocarbonetos/química
Oxigênio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons); 0 (Thiophenes); 0 (Water Pollutants, Chemical); S88TT14065 (Oxygen); Y5L06AH4G5 (sulfolane)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181462



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