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[PMID]:29258487
[Au] Autor:Ferrari C; Salle R; Callemeyn-Torre N; Jovelin R; Cutter AD; Braendle C
[Ad] Endereço:CNRS, IBV, Inserm, Université Côte d'Azur, Nice, France.
[Ti] Título:Ephemeral-habitat colonization and neotropical species richness of Caenorhabditis nematodes.
[So] Source:BMC Ecol;17(1):43, 2017 Dec 19.
[Is] ISSN:1472-6785
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The drivers of species co-existence in local communities are especially enigmatic for assemblages of morphologically cryptic species. Here we characterize the colonization dynamics and abundance of nine species of Caenorhabditis nematodes in neotropical French Guiana, the most speciose known assemblage of this genus, with resource use overlap and notoriously similar external morphology despite deep genomic divergence. METHODS: To characterize the dynamics and specificity of colonization and exploitation of ephemeral resource patches, we conducted manipulative field experiments and the largest sampling effort to date for Caenorhabditis outside of Europe. This effort provides the first in-depth quantitative analysis of substrate specificity for Caenorhabditis in natural, unperturbed habitats. RESULTS: We amassed a total of 626 strain isolates from nine species of Caenorhabditis among 2865 substrate samples. With the two new species described here (C. astrocarya and C. dolens), we estimate that our sampling procedures will discover few additional species of these microbivorous animals in this tropical rainforest system. We demonstrate experimentally that the two most prevalent species (C. nouraguensis and C. tropicalis) rapidly colonize fresh resource patches, whereas at least one rarer species shows specialist micro-habitat fidelity. CONCLUSION: Despite the potential to colonize rapidly, these ephemeral patchy resources of rotting fruits and flowers are likely to often remain uncolonized by Caenorhabditis prior to their complete decay, implying dispersal-limited resource exploitation. We hypothesize that a combination of rapid colonization, high ephemerality of resource patches, and species heterogeneity in degree of specialization on micro-habitats and life histories enables a dynamic co-existence of so many morphologically cryptic species of Caenorhabditis.
[Mh] Termos MeSH primário: Distribuição Animal
Caenorhabditis/fisiologia
Ecossistema
[Mh] Termos MeSH secundário: Animais
Biota
Guiana Francesa
Densidade Demográfica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1186/s12898-017-0150-z


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[PMID]:29390455
[Au] Autor:Felix A; Hallet E; Favre A; Kom-Tchameni R; Defo A; Fléchelles O; Rosenthal JM; Douine M; Nacher M; Elenga N
[Ad] Endereço:Department of Pediatrics, Andrée Rosemon Hospital.
[Ti] Título:Cerebral injuries associated with Zika virus in utero exposure in children without birth defects in French Guiana: Case report.
[So] Source:Medicine (Baltimore);96(51):e9178, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: A major epidemic of Zika virus (ZIKV) infection occurred in French Guiana and West Indies. French national epidemiological surveillance estimated that 1650 pregnant women contracted the ZIKV during epidemic period from January 2016 to October 2016 in French Guiana. PATIENT CONCERNS: ZIKV infection during pregnancy is a cause of microcephaly and birth defects. DIAGNOSES: In this report, we describe 2 children with proven in utero ZIKV exposure. Their mothers were both symptomatic and ZIKV infection occurred early in pregnancy. Ultrasonography monitoring in utero did not show any abnormality for both patient. They were born at full-term, healthy, without any birth defects and no sign of congenital ZIKV infection. INTERVENTIONS: ZIKV was neither found on placenta fragments nor children blood and urine at birth. Their neurodevelopment outcomes in early-life fitted the expectations. As recommended in national guidelines, we performed cerebral MRIs at 2 months old, showing severe brain abnormalities, especially of white matter areas. After a large screening, we did not find any differential diagnosis for their brain lesions. OUTCOMES: We concluded it was due to their in utero ZIKV exposure. LESSONS: In this report, pathogenicity of ZIKV may involve mother's immunological response or metabolic disorder during the infection.
[Mh] Termos MeSH primário: Lesões Encefálicas/virologia
Imagem por Ressonância Magnética/métodos
Complicações Infecciosas na Gravidez/diagnóstico por imagem
Efeitos Tardios da Exposição Pré-Natal/diagnóstico por imagem
Infecção pelo Zika virus/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Lesões Encefálicas/diagnóstico por imagem
Lesões Encefálicas/etiologia
Cesárea
Desenvolvimento Infantil/fisiologia
Feminino
Seguimentos
Guiana Francesa
Seres Humanos
Lactente
Recém-Nascido
Masculino
Gravidez
Complicações Infecciosas na Gravidez/virologia
Efeitos Tardios da Exposição Pré-Natal/patologia
Medição de Risco
Ultrassonografia Pré-Natal/métodos
Infecção pelo Zika virus/complicações
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009178


  3 / 836 MEDLINE  
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Machado, Ricardo Luiz Dantas
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[PMID]:29293589
[Au] Autor:Menezes RAO; Gomes MDSM; Mendes AM; Couto ÁARA; Nacher M; Pimenta TS; Sousa ACP; Baptista ARS; Jesus MI; Enk MJ; Cunha MG; Machado RLD
[Ad] Endereço:Postgraduate Program in the Biology of Infectious and Parasitic Agents, Federal University of Pará (UFPA), Belém, Pará State, Brazil.
[Ti] Título:Enteroparasite and vivax malaria co-infection on the Brazil-French Guiana border: Epidemiological, haematological and immunological aspects.
[So] Source:PLoS One;13(1):e0189958, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Malaria-enteroparasitic co-infections are known for their endemicity. Although they are prevalent, little is known about their epidemiology and effect on the immune response. This study evaluated the effect of enteroparasite co-infections with malaria caused by Plasmodium vivax in a border area between Brazil and French Guiana. The cross sectional study took place in Oiapoque, a municipality of Amapá, on the Amazon border. Malaria was diagnosed using thick blood smears, haemoglobin dosage by an automated method and coproparasitology by the Hoffman and Faust methods. The anti-PvMSP-119 IgG antibodies in the plasma were evaluated using ELISA and Th1 (IFN-γ, TNF-α and IL-2), and Th2 (IL-4, IL-5 and IL-10) cytokine counts were performed by flow cytometry. The participants were grouped into those that were monoinfected with vivax malaria (M), vivax malaria-enteroparasite co-infected (CI), monoinfected with enteroparasite (E) and endemic controls (EC), who were negative for both diseases. 441 individuals were included and grouped according to their infection status: [M 6.9% (30/441)], [Cl 26.5% (117/441)], [E 32.4% (143/441)] and [EC 34.2% (151/441)]. Males prevailed among the (M) 77% (23/30) and (CI) 60% (70/117) groups. There was a difference in haemoglobin levels among the different groups under study for [EC-E], [EC-Cl], [E-M] and [Cl-M], with (p < 0.01). Anaemia was expressed as a percentage between individuals [CI-EC (p < 0.05)]. In terms of parasitaemia, there were differences for the groups [CI-M (p < 0.05)]. Anti-PvMSP-119 antibodies were detected in 51.2% (226/441) of the population. The level of cytokines evaluation revealed a large variation in TNF-α and IL-10 concentrations in the co-infected group. In this study we did not observe any influence of coinfection on the acquisition of IgG antibodies against PvMSP119, as well as on the profile of the cytokines that characterize the Th1 and Th2 patterns. However, co-infection increased TNF-α and IL-10 levels.
[Mh] Termos MeSH primário: Malária Vivax/epidemiologia
Doenças Parasitárias/epidemiologia
[Mh] Termos MeSH secundário: Animais
Brasil/epidemiologia
Estudos Transversais
Citocinas/sangue
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Guiana Francesa/epidemiologia
Seres Humanos
Imunoglobulina G/imunologia
Malária Vivax/sangue
Malária Vivax/complicações
Doenças Parasitárias/sangue
Doenças Parasitárias/complicações
Proteínas de Protozoários/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Immunoglobulin G); 0 (Protozoan Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189958


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[PMID]:28464856
[Au] Autor:Ayhan G; Arnal R; Basurko C; About V; Pastre A; Pinganaud E; Sins D; Jehel L; Falissard B; Nacher M
[Ad] Endereço:Inserm CIC 1424, Centre d'Investigation Clinique Antilles Guyane, Centre Hospitalier de Cayenne, Avenue des Flamboyants, BP 6006, 97 306, Cayenne CEDEX, France. guelen.ayhan@gmail.com.
[Ti] Título:Suicide risk among prisoners in French Guiana: prevalence and predictive factors.
[So] Source:BMC Psychiatry;17(1):156, 2017 05 02.
[Is] ISSN:1471-244X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Suicide rates in prison are high and their risk factors are incompletely understood. The objective of the present study is to measure the risk of suicide and its predictors in the only prison of multicultural French Guiana. METHODS: All new prisoners arriving between September 2013 and December 2014 were included. The Mini International Neuropsychiatric Interview (MINI) was used and socio-demographic data was collected. In order to identify the predictors of suicide risk multivariate logistic regression was used. RESULTS: Of the 707 prisoners included 13.2% had a suicidal risk, 14.0% of whom had a high risk, 15.1% a moderate risk and 41.9% a low risk. Predictive factors were depression (OR 7.44, 95% CI: 3.50-15.87), dysthymia (OR 4.22, 95% CI: 1.34-13.36), panic disorder (OR 3.47, 95% CI: 1.33-8.99), general anxiety disorder (GAD) (OR 2.19, 95% CI: 1.13-4.22), men having been abused during childhood (OR 21.01, 95%, CI: 3.26-135.48), having been sentenced for sexual assault (OR 7.12, 95% CI: 1.98-25.99) and smoking (OR 2.93, 95%, CI 1.30-6.63). CONCLUSION: The suicide risk was lower than in mainland France, possibly reflecting the differences in the social stigma attached to incarceration because of migrant populations and the importance and trivialization of drug trafficking among detainees. However, there were no differences between nationalities. The results reemphasize the importance of promptly identifying and treating psychiatric disorders, which were the main suicide risk factors.
[Mh] Termos MeSH primário: Transtornos Mentais/epidemiologia
Prisioneiros/psicologia
Suicídio/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Estudos Transversais
Grupos Étnicos
Feminino
Guiana Francesa/epidemiologia
Seres Humanos
Entrevistas como Assunto
Modelos Logísticos
Masculino
Transtornos Mentais/etnologia
Transtornos Mentais/psicologia
Meia-Idade
Prevalência
Prisões
Psicometria
Fatores de Risco
Suicídio/etnologia
Suicídio/psicologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12888-017-1320-4


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[PMID]:29100086
[Au] Autor:Fortes-Lima C; Gessain A; Ruiz-Linares A; Bortolini MC; Migot-Nabias F; Bellis G; Moreno-Mayar JV; Restrepo BN; Rojas W; Avendaño-Tamayo E; Bedoya G; Orlando L; Salas A; Helgason A; Gilbert MTP; Sikora M; Schroeder H; Dugoujon JM
[Ad] Endereço:Laboratoire d'Anthropologie Moléculaire et Imagerie de Synthèse, AMIS UMR5288, Centre National de la Recherche Scientifique (CNRS) -Université Paul Sabatier Toulouse III, Toulouse 31000, France; Laboratory Eco-Anthropology and Ethno-Biology, UMR7206, CNRS-MNHN-University Paris Diderot, Musée de l'Ho
[Ti] Título:Genome-wide Ancestry and Demographic History of African-Descendant Maroon Communities from French Guiana and Suriname.
[So] Source:Am J Hum Genet;101(5):725-736, 2017 Nov 02.
[Is] ISSN:1537-6605
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The transatlantic slave trade was the largest forced migration in world history. However, the origins of the enslaved Africans and their admixture dynamics remain unclear. To investigate the demographic history of African-descendant Marron populations, we generated genome-wide data (4.3 million markers) from 107 individuals from three African-descendant populations in South America, as well as 124 individuals from six west African populations. Throughout the Americas, thousands of enslaved Africans managed to escape captivity and establish lasting communities, such as the Noir Marron. We find that this population has the highest proportion of African ancestry (∼98%) of any African-descendant population analyzed to date, presumably because of centuries of genetic isolation. By contrast, African-descendant populations in Brazil and Colombia harbor substantially more European and Native American ancestry as a result of their complex admixture histories. Using ancestry tract-length analysis, we detect different dates for the European admixture events in the African-Colombian (1749 CE; confidence interval [CI]: 1737-1764) and African-Brazilian (1796 CE; CI: 1789-1804) populations in our dataset, consistent with the historically attested earlier influx of Africans into Colombia. Furthermore, we find evidence for sex-specific admixture patterns, resulting from predominantly European paternal gene flow. Finally, we detect strong genetic links between the African-descendant populations and specific source populations in Africa on the basis of haplotype sharing patterns. Although the Noir Marron and African-Colombians show stronger affinities with African populations from the Bight of Benin and the Gold Coast, the African-Brazilian population from Rio de Janeiro has greater genetic affinity with Bantu-speaking populations from the Bight of Biafra and west central Africa.
[Mh] Termos MeSH primário: Grupo com Ancestrais do Continente Africano/genética
[Mh] Termos MeSH secundário: África
Brasil
Grupo com Ancestrais do Continente Europeu/genética
Feminino
Guiana Francesa
Fluxo Gênico/genética
Genética Populacional
Estudo de Associação Genômica Ampla/métodos
Haplótipos
Hispano-Americanos/genética
Seres Humanos
Masculino
Polimorfismo de Nucleotídeo Único/genética
Suriname
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171104
[St] Status:MEDLINE


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[PMID]:28727841
[Au] Autor:Adler PH; Hamada N; Cavalcante do Nascimento JM; Grillet ME
[Ad] Endereço:Department of Plant and Environmental Sciences, Clemson University, Clemson, South Carolina, United States of America.
[Ti] Título:River-specific macrogenomic diversity in Simulium guianense s. l. (Diptera: Simuliidae), a complex of tropical American vectors associated with human onchocerciasis.
[So] Source:PLoS One;12(7):e0181679, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Simulium guianense Wise is a Latin American vector complex of black flies associated with transmission of the causal agent of human onchocerciasis (river blindness). An analysis of the chromosomal banding patterns of 607 larvae of S. guianense s. l. revealed a high level of variation involving 83 macrogenomic rearrangements across 25 populations in Brazil, French Guiana, and Venezuela. The 25 populations were assigned to 13 cytoforms (A1, A2, B1-B4, C, D, E1-E4, and F), some of which are probably valid species. Based on geographical proximity, a member of the B group of cytoforms probably represents the name-bearing type specimen of S. guianense and the primary vector in the last-remaining onchocerciasis foci in the Western Hemisphere. Cytoform B3 in Amapá State is implicated as an anthropophilic simuliid in an area currently and historically free of onchocerciasis. Distributions of cytoforms are associated with geography, elevation, and drainage basin, and are largely congruent with ecoregions. Despite extraordinarily large larval populations of S. guianense s. l. in big rivers and consequent production of female flies for dispersal, the cytoforms maintain their chromosomal distinction within individual rivers, suggesting a high degree of fidelity to the specialized breeding habitats-rocky shoals-of the natal rivers.
[Mh] Termos MeSH primário: Ecótipo
Insetos Vetores/genética
Rios
Simuliidae/genética
[Mh] Termos MeSH secundário: Distribuição Animal
Animais
Evolução Biológica
Brasil
Cromossomos de Insetos
Classificação
Guiana Francesa
Geografia
Seres Humanos
Larva/genética
Oncocercose/transmissão
Comportamento Sexual Animal
Especificidade da Espécie
Venezuela
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181679


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[PMID]:28722641
[Au] Autor:Ölschläger S; Enfissi A; Zaruba M; Kazanji M; Rousset D
[Ad] Endereço:altona Diagnostics GmbH, Hamburg, Germany.
[Ti] Título:Diagnostic Validation of the RealStar Zika Virus Reverse Transcription Polymerase Chain Reaction Kit for Detection of Zika Virus RNA in Urine and Serum Specimens.
[So] Source:Am J Trop Med Hyg;97(4):1070-1071, 2017 Oct.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:With the Zika virus outbreak in South America starting in 2015 and its potential to cause malformation of the fetus in infected women, the need for diagnostic methods became obvious. Until now, only limited data are available on the diagnostic performance of commercial kits. Here, we present data comparing the RealStar Zika Virus RT-PCR Kit 1.0 for detection of Zika virus from 208 serum and urine samples collected in French Guiana with a reference method. Of these, 114 samples tested positive with the RealStar Kit and 111 with the reference method.
[Mh] Termos MeSH primário: Kit de Reagentes para Diagnóstico
Soro/virologia
Infecção pelo Zika virus/sangue
Infecção pelo Zika virus/urina
Zika virus/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Guiana Francesa/epidemiologia
Seres Humanos
Masculino
Meia-Idade
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Infecção pelo Zika virus/epidemiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.17-0268


  8 / 836 MEDLINE  
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[PMID]:28722640
[Au] Autor:Flamand C; Camille Fritzell; Pauline Obale; Quenel P; Raude J
[Ad] Endereço:Unité d'Épidémiologie, Institut Pasteur de la Guyane, Cayenne, France.
[Ti] Título:The Role of Risk Proximity in the Beliefs and Behaviors Related to Mosquito-Borne Diseases: The Case of Chikungunya in French Guiana.
[So] Source:Am J Trop Med Hyg;97(2):344-355, 2017 Aug.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human behaviors are increasingly recognized to play a key role in the spread of infectious diseases. Although a set of social and cognitive determinants has been consistently found to affect the adoption of health protective behaviors aiming to control and prevent a variety of infections, little is currently known about the ecological drivers of these behaviors in epidemic settings. In this article, we took advantage of the outbreak of chikungunya, a reemerging mosquito-borne disease, that occurred in French Guiana in 2014-15 to test empirically the assumption proposed by Zielinski-Gutierrez and Hayden that the proximity of the disease and perceptions of the natural environment may considerably shape public response to an emerging health threat. To achieve this, a cross-sectional survey was conducted among high school students of the region ( = 1462) at an early stage of the epidemic. Surprisingly, spatial analysis of the collected data leads to counterintuitive results as the participants who lived in the most affected area expressed less concern about the disease and practiced preventive behaviors less frequently than did other participants. These paradoxical results may be attributed to the possible activation of risk denial processes which have previously been observed in the risk perception literature, and described by several social and psychological defensiveness theories.
[Mh] Termos MeSH primário: Comportamento do Adolescente/psicologia
Atitude Frente à Saúde
Febre de Chikungunya/prevenção & controle
Febre de Chikungunya/psicologia
Doenças Transmissíveis/psicologia
Conhecimentos, Atitudes e Prática em Saúde
Estudantes/psicologia
[Mh] Termos MeSH secundário: Adolescente
Febre de Chikungunya/epidemiologia
Doenças Transmissíveis/epidemiologia
Estudos Transversais
Surtos de Doenças
Feminino
Guiana Francesa/epidemiologia
Seres Humanos
Masculino
Prevalência
Medição de Risco
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-1028


  9 / 836 MEDLINE  
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[PMID]:28715422
[Au] Autor:Tirera S; Ginouves M; Donato D; Caballero IS; Bouchier C; Lavergne A; Bourreau E; Mosnier E; Vantilcke V; Couppié P; Prevot G; Lacoste V
[Ad] Endereço:Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana.
[Ti] Título:Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana.
[So] Source:PLoS Negl Trop Dis;11(7):e0005764, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Leishmania RNA virus type 1 (LRV1) is an endosymbiont of some Leishmania (Vianna) species in South America. Presence of LRV1 in parasites exacerbates disease severity in animal models and humans, related to a disproportioned innate immune response, and is correlated with drug treatment failures in humans. Although the virus was identified decades ago, its genomic diversity has been overlooked until now. METHODOLOGY/PRINCIPLES FINDINGS: We subjected LRV1 strains from 19 L. (V.) guyanensis and one L. (V.) braziliensis isolates obtained from cutaneous leishmaniasis samples identified throughout French Guiana with next-generation sequencing and de novo sequence assembly. We generated and analyzed 24 unique LRV1 sequences over their full-length coding regions. Multiple alignment of these new sequences revealed variability (0.5%-23.5%) across the entire sequence except for highly conserved motifs within the 5' untranslated region. Phylogenetic analyses showed that viral genomes of L. (V.) guyanensis grouped into five distinct clusters. They further showed a species-dependent clustering between viral genomes of L. (V.) guyanensis and L. (V.) braziliensis, confirming a long-term co-evolutionary history. Noteworthy, we identified cases of multiple LRV1 infections in three of the 20 Leishmania isolates. CONCLUSIONS/SIGNIFICANCE: Here, we present the first-ever estimate of LRV1 genomic diversity that exists in Leishmania (V.) guyanensis parasites. Genetic characterization and phylogenetic analyses of these viruses has shed light on their evolutionary relationships. To our knowledge, this study is also the first to report cases of multiple LRV1 infections in some parasites. Finally, this work has made it possible to develop molecular tools for adequate identification and genotyping of LRV1 strains for diagnostic purposes. Given the suspected worsening role of LRV1 infection in the pathogenesis of human leishmaniasis, these data have a major impact from a clinical viewpoint and for the management of Leishmania-infected patients.
[Mh] Termos MeSH primário: Variação Genética
Leishmania/virologia
Leishmaniavirus/classificação
Leishmaniavirus/isolamento & purificação
Filogenia
[Mh] Termos MeSH secundário: Adulto
Idoso
Análise por Conglomerados
Feminino
Guiana Francesa
Genoma Viral
Seres Humanos
Leishmania/isolamento & purificação
Leishmaniose/parasitologia
Leishmaniavirus/genética
Masculino
Meia-Idade
RNA Viral/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005764


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[PMID]:28644767
[Au] Autor:Rimbaud D; Restrepo M; Louison A; Boukhari R; Ardillon V; Carles G; Lambert V; Jolivet A
[Ad] Endereço:a Department of Public Health , Centre Hospitalier de l'Ouest Guyanais , Saint-Laurent du Maroni , French Guiana.
[Ti] Título:Blood lead levels and risk factors for lead exposure among pregnant women in western French Guiana: the role of manioc consumption.
[So] Source:J Toxicol Environ Health A;80(6):382-393, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Concerns regarding lead (Pb) poisoning in French Guiana first arose in 2011 following the discovery of excessively high levels of the metal amongst children in a small neighborhood without any apparent source of Pb. Since 2012, blood lead level (BLL) measurement has been proposed for all pregnant women in western French Guiana. The aim of this study was to determine BLL in pregnant women in this region and identify factors associated with elevated BLL. An observational study of a consecutive sample of women who delivered in the maternity ward of the hospital was conducted. Risk factors were investigated using a questionnaire administered postdelivery by midwives (N = 531). Approximately 25 and 5% of women displayed BLL of ≥50 µg/L and ≥100 µg/L, respectively. The geometric mean was 32.6 µg/L. Factors that were significantly associated with an elevated BLL after modeling (multivariate linear regression) included place of residence along the Maroni river, low level of education, daily consumption of manioc derivatives, weekly and daily consumption or personal preparation of manioc flour during pregnancy, and weekly consumption of wild game. This study provides insight into the regional and social disparities in BLL in French Guiana and potential sources of exposure. Evidence indicates that foods that are primarily produced and consumed in the Guiana Shield significantly affect BLL levels. Taken together with existing data, our results demonstrate that specific actions in terms of prevention, screening, and care are required to be adapted and put into place in order to reduce exposure.
[Mh] Termos MeSH primário: Dieta
Exposição Ambiental
Intoxicação por Chumbo/sangue
Manihot/química
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estudos Transversais
Monitoramento Ambiental
Feminino
Guiana Francesa
Seres Humanos
Gravidez
Fatores de Risco
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1331490



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