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  1 / 1027 MEDLINE  
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[PMID]:28745599
[Au] Autor:Samoutis G; Paschalides C
[Ad] Endereço:University of Nicosia Medical School, Engomi, Nicosia, 1700, Cyprus. Electronic address: samoutis.g@unic.ac.cy.
[Ti] Título:The sun is shining on Cyprus's National Health Service.
[So] Source:Lancet;390(10092):360, 2017 07 22.
[Is] ISSN:1474-547X
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Reforma dos Serviços de Saúde/organização & administração
Programas Nacionais de Saúde/organização & administração
[Mh] Termos MeSH secundário: Chipre
Assistência à Saúde/organização & administração
Assistência à Saúde/tendências
Seres Humanos
Programas Nacionais de Saúde/tendências
Medicina Estatal/organização & administração
Medicina Estatal/tendências
[Pt] Tipo de publicação:LETTER
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


  2 / 1027 MEDLINE  
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[PMID]:28448779
[Au] Autor:Korcegez EI; Sancar M; Demirkan K
[Ad] Endereço:1 Department of Clinical Pharmacy, Near East University Faculty of Pharmacy, Nicosia, Northern Cyprus.
[Ti] Título:Effect of a Pharmacist-Led Program on Improving Outcomes in Patients with Type 2 Diabetes Mellitus from Northern Cyprus: A Randomized Controlled Trial.
[So] Source:J Manag Care Spec Pharm;23(5):573-582, 2017 May.
[Is] ISSN:2376-1032
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The management of type 2 diabetes mellitus is complex, requiring continuous medical care by health care professionals and considerable self-care efforts by patients. Pharmacist-led care programs have been shown to help patients with diabetes succeed in achieving treatment goals and improving outcomes. Pharmacist-led care is a new health care concept in Northern Cyprus. OBJECTIVE: To evaluate the effect of a pharmacist-led care program on glycemic control, determined by hemoglobin A1c (A1c), and secondarily on blood pressure, lipid profile, body mass index (BMI), waist circumference, medication adherence, and self-care activities, for patients with type 2 diabetes over a 12-month period. METHODS: This was a prospective, randomized controlled study conducted in a public hospital's outpatient diabetes clinic, with 152 patients who had been diagnosed with type 2 diabetes. Of these, 75 patients were in the intervention group, and 77 patients were in the usual care group. The intervention group participated in a pharmacist-led care program with a clinical pharmacist who provided 5 face-to-face educational sessions over a period of 12 months. The main outcome measure was change in A1c, and secondary outcome measures were changes in fasting blood glucose, systolic and diastolic blood pressure, lipid values (total cholesterol, low-density lipoprotein cholesterol [LDL-C], high-density lipoprotein cholesterol [HDL-C], and serum levels of triglycerides [TGs]), BMI, waist circumference, self-reported medication adherence (Morisky-Green test), and self-care activities. Changes in outcome measures from baseline to the end of the study were assessed using the Mann-Whitney U-test and Wilcoxon test. RESULTS: At the end of the 12-month study period, the intervention patients showed a greater reduction in A1c values than the usual care patients (-0.74% vs. -0.04%; P < 0.001). Both groups showed significant reductions in fasting blood glucose levels between baseline and the end of 12 months; the difference between the groups was statistically nonsignificant (P = 0.410). When comparing the intervention and usual care groups, there was a significant decrease in systolic (P = 0.01) and diastolic blood pressure (P = 0.04) at the end of the trial. No significant differences were found between the groups in LDL-C, HDL-C, or TG values; however, total cholesterol levels did decrease significantly (P = 0.063, 0.331, 0.896, and 0.04, respectively). Significant reductions occurred in BMI (P < 0.001) and waist circumference (P < 0.001), and improvements were observed in self-reported medication adherence and self-care activities in the intervention group. CONCLUSIONS: A clinical pharmacist-led care program in a public hospital's outpatient diabetes clinic was associated with significant improvements in reducing A1c and other secondary outcomes in a 12-month randomized controlled study. DISCLOSURES: This study was conducted as a PhD thesis by Korcegez under the supervision of Sancar for the clinical pharmacy program at Near East University, Health Sciences Institute, Northern Cyprus, and received no external funding. The authors have no potential conflicts of interest to report. Study concept and design were contributed by Korcegez, with assistance from Sancar and Demirkan. Korcegez took the lead in data collection, and data interpretation was performed by Korcegez, along with Sancar and Korcegez. The manuscript was written and revised by Korcegez, along with Sancar, and with assistance from Demirkan.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/terapia
Hemoglobina A Glicada/metabolismo
Assistência Farmacêutica/organização & administração
Farmacêuticos/organização & administração
[Mh] Termos MeSH secundário: Idoso
Glicemia
Pressão Sanguínea
Chipre
Feminino
Seres Humanos
Masculino
Adesão à Medicação
Meia-Idade
Avaliação de Resultados (Cuidados de Saúde)
Pacientes Ambulatoriais
Papel Profissional
Estudos Prospectivos
Autocuidado
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Glycated Hemoglobin A)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.18553/jmcp.2017.23.5.573


  3 / 1027 MEDLINE  
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[PMID]:28895674
[Au] Autor:Wadas W; Królak E; Karwowska J
[Ad] Endereço:Siedlce University of Natural Sciences and Humanities, Faculty of Natural Science, Department of Vegetable Crops, Siedlce, Poland
[Ti] Título:Activity of cesium 137Cs and potassium content in new potatoes imported to Poland
[So] Source:Rocz Panstw Zakl Hig;68(3):297-302, 2017.
[Is] ISSN:0035-7715
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Background: Potatoes are an important component of the human diet. In addition to components which determine the nutrition and dietary values, potato tubers also contain anti-nutritional substances, inter alia radioactive elements. Natural and artifical radionuclides are released to the environment as a result of antropogenic activity, in a controlled or uncontrolled manner, and they are transferred to the human body through the food chain. Objective: The aim of the study was to determine the activity of radioactive cesium 137Cs isotope and potassium content, including the activity of 40K isotope, in new potatoes imported to Poland during the winter period from Mediterranean countries. Material and methods: The study material included new potatoes imported from Cyprus, Egypt and Israel, purchased in the city of Siedlce from the beginning of February to the end of March 2015. The activity of 137Cs and 40K isotopes in potato tubers was determined. Analyses were performed by γ-spectrometric method. Laboratory tests were performed on a total of 18 samples. Based on the activity of 40K isotope, the total potassium content of potato tubers was calculated, with the assumption that 31.00 Bq 40K is equivalent to 1 g potassium. Results: The activity of 137Cs in most tested potato samples was below 0.2 Bq kg-1 (limit of quantification), and in other samles it was from 0.3 Bq kg-1 to 5.4 Bq kg-1. Potatoes of the same variety, originating from the same country, differed in terms of the activity of 137Cs. The highest activity of 137C, determined in potatoes imported from Cyprus, was seven times higher than the lowest value. The activity of 40K changed from 93.3 Bq kg-1 to 259.1 Bq kg-1. The average activity of 40K in potatoes imported from Cyprus, Egypt and Israel was at a similar level. The ratio of the activity of 137Cs determined in the tested potatoes to the activity of 40K changed from 0.00242 to 0.04163. The calculated potassium content in imported new potatoes was on average 4.376 g K kg-1 of the fresh weight of tubers and ranged from 3.010 g K kg-1 to 8.358 g K kg-1. Conclusions: The activity of the 137Cs cesium isotope in imported new potatoes in most tested samples was at a very low level (below the limit of quantification) and in other samples it did not exceed 5.5 Bq kg-1 and posed no threat to human lives. Potatoes originating from the same country differed in terms of the activity of 137Cs. The average activity of 40K in potatoes imported from Cyprus, Egypt and Israel was at a similar level and did not differ from the activity of 40K in domestically produced potatoes. The potassium content in imported new potatoes was determined by the variety.
[Mh] Termos MeSH primário: Radioisótopos de Césio/análise
Contaminação Radioativa de Alimentos/análise
Radioisótopos de Potássio/análise
Poluentes Radioativos/análise
Solanum tuberosum/química
[Mh] Termos MeSH secundário: Chipre
Egito
Análise de Alimentos/métodos
Israel
Polônia
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cesium Radioisotopes); 0 (Potassium Radioisotopes); 0 (Radioactive Pollutants)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE


  4 / 1027 MEDLINE  
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[PMID]:28850572
[Au] Autor:Jimenez C; Andreou V; Evriviadou M; Munkes B; Hadjioannou L; Petrou A; Abu Alhaija R
[Ad] Endereço:Enalia Physis Environmental Research Centre (ENALIA), Acropoleos 2, Aglanzia, Nicosia, Cyprus.
[Ti] Título:Epibenthic communities associated with unintentional artificial reefs (modern shipwrecks) under contrasting regimes of nutrients in the Levantine Sea (Cyprus and Lebanon).
[So] Source:PLoS One;12(8):e0182486, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Artificial reefs, in the Eastern Mediterranean (Cyprus,) became a popular and frequently used tool, in fisheries and biodiversity conservation management. Even though evaluation studies about the efficacy of artificial reefs are plentiful in the rest of the Mediterranean (Central and Western), in the Eastern Basin they are largely absent. As the Eastern part of the Mediterranean Sea is characterised by unique physical parameters, the necessity to study artificial reefs under these contrasting regimes increases. The epibenthic communities of two unintentional artificial reefs (modern shipwrecks) in Cyprus (Zenobia) and Lebanon (Alice-B) were evaluated in 2010. Both shipwrecks are at similar depth, type of sea bottom, made of the same material (steel) and were sunk approximately the same period of time. However, Alice-B shipwreck off the coast of Lebanon is constantly exposed to higher levels of nutrients than Zenobia in Cyprus. Significant dissimilarities were observed in the composition, percentage of benthic cover of predominant taxonomic groups and development of the epibenthic communities. Differences in physical and chemical parameters between sides lay mainly in the nutrient and thermal regimes affecting the shipwrecks and most likely bring about the differences in the observed community structure. The results of this study suggest that epibenthic communities could be highly impacted by eutrophication caused by anthropogenic activities, leading to less biodiverse communities dominated by specific species that are favoured by the eutrophic conditions.
[Mh] Termos MeSH primário: Biodiversidade
Recifes de Corais
Ecossistema
Eutrofização
[Mh] Termos MeSH secundário: Animais
Antozoários/crescimento & desenvolvimento
Chipre
Líbano
Mar Mediterrâneo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182486


  5 / 1027 MEDLINE  
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[PMID]:28648287
[Au] Autor:Pantelides IS; Aristeidou E; Lazari M; Tsolakidou MD; Tsaltas D; Christofidou M; Kafouris D; Christou E; Ioannou N
[Ad] Endereço:Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Limassol, Cyprus. Electronic address: iakovos.pantelides@cut.ac.cy.
[Ti] Título:Biodiversity and ochratoxin A profile of Aspergillus section Nigri populations isolated from wine grapes in Cyprus vineyards.
[So] Source:Food Microbiol;67:106-115, 2017 Oct.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to evaluate the biodiversity of Aspergillus section Nigri populations from Cyprus vineyards by morphological, toxigenic and phylogenetic analysis. Aspergillus section Nigri populations were isolated from grapes of the varieties 'Maratheftiko' and 'Cabernet Sauvignon' originating from six growing regions of Cyprus during 2010 and 2011 years. The isolation frequency of Aspergillus section Nigri from grape samples was 43.3% and a total of 284 isolates were selected for further analyses based on the macroscopic characteristics of black aspergilli. The isolates were characterized by sequencing analysis of the calmodulin gene in order to identify species responsible for ochratoxin A (OTA) production. The phylogenetic analysis showed that the isolates were grouped in three major clusters. The A. tubingensis cluster included 262 isolates (92.25%), the A. niger cluster included 15 isolates identified as A. niger (5.3%) and 6 isolates identified as A. welwitschiae (2.1%). One isolate was classified as A. carbonarius (0.35%) and was grouped in a cluster together with the reference isolates of A. carbonarius, A. sclerotioniger, A. sclerotiocarbonarius and A. ibericus. All the isolates were evaluated for their ochratoxigenic ability by HPLC coupled with a fluorescence detector (HPLC-FLD) and the positive isolates were re-examined using ultra high-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The Aspergillus carbonarius isolate produced an average quantity of 1436.1 ng OTA/g Czapek Yeast Agar (CYA); From the A. niger strains three isolates (20%) produced OTA and only one isolate from A. welwitschiae (16.7%) was proved ochratoxigenic with toxin production average at 23.9 ng/g and 9.1 ng/g CYA respectively. Grape must samples derived from the collected berries were also analyzed for OTA and none of the samples were found contaminated with the mycotoxin. The results showed that the geographic area and the meteorological conditions had no significant effect on the incidence and the distribution of black aspergilli in this 2-year project. However, absence of rainfall and low humidity during the harvesting period were critical for the low incidence of the ochratoxigenic A. carbonarius on grapes.
[Mh] Termos MeSH primário: Aspergillus/isolamento & purificação
Biodiversidade
Ocratoxinas/biossíntese
Vitis/microbiologia
[Mh] Termos MeSH secundário: Aspergillus/classificação
Aspergillus/genética
Aspergillus/metabolismo
Chipre
Contaminação de Alimentos/análise
Frutas/microbiologia
Filogenia
Vinho/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ochratoxins); 1779SX6LUY (ochratoxin A)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


  6 / 1027 MEDLINE  
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[PMID]:28622394
[Au] Autor:Heraclides A; Bashiardes E; Fernández-Domínguez E; Bertoncini S; Chimonas M; Christofi V; King J; Budowle B; Manoli P; Cariolou MA
[Ad] Endereço:Department of Cardiovascular Genetics and The Laboratory of Forensic Genetics, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus.
[Ti] Título:Y-chromosomal analysis of Greek Cypriots reveals a primarily common pre-Ottoman paternal ancestry with Turkish Cypriots.
[So] Source:PLoS One;12(6):e0179474, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genetics can provide invaluable information on the ancestry of the current inhabitants of Cyprus. A Y-chromosome analysis was performed to (i) determine paternal ancestry among the Greek Cypriot (GCy) community in the context of the Central and Eastern Mediterranean and the Near East; and (ii) identify genetic similarities and differences between Greek Cypriots (GCy) and Turkish Cypriots (TCy). Our haplotype-based analysis has revealed that GCy and TCy patrilineages derive primarily from a single gene pool and show very close genetic affinity (low genetic differentiation) to Calabrian Italian and Lebanese patrilineages. In terms of more recent (past millennium) ancestry, as indicated by Y-haplotype sharing, GCy and TCy share much more haplotypes between them than with any surrounding population (7-8% of total haplotypes shared), while TCy also share around 3% of haplotypes with mainland Turks, and to a lesser extent with North Africans. In terms of Y-haplogroup frequencies, again GCy and TCy show very similar distributions, with the predominant haplogroups in both being J2a-M410, E-M78, and G2-P287. Overall, GCy also have a similar Y-haplogroup distribution to non-Turkic Anatolian and Southwest Caucasian populations, as well as Cretan Greeks. TCy show a slight shift towards Turkish populations, due to the presence of Eastern Eurasian (some of which of possible Ottoman origin) Y-haplogroups. Overall, the Y-chromosome analysis performed, using both Y-STR haplotype and binary Y-haplogroup data puts Cypriot in the middle of a genetic continuum stretching from the Levant to Southeast Europe and reveals that despite some differences in haplotype sharing and haplogroup structure, Greek Cypriots and Turkish Cypriots share primarily a common pre-Ottoman paternal ancestry.
[Mh] Termos MeSH primário: Cromossomos Humanos Y/genética
Grupo com Ancestrais do Continente Europeu/etnologia
Grupo com Ancestrais do Continente Europeu/genética
Haplótipos
[Mh] Termos MeSH secundário: Chipre/etnologia
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179474


  7 / 1027 MEDLINE  
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[PMID]:28511636
[Au] Autor:Krashias G; Koptides D; Christodoulou C
[Ad] Endereço:Department of Molecular Virology, The Cyprus Institute of Neurology and Genetics, 6 International Airport Avenue, 2370, Nicosia, Cyprus. georgek@cing.ac.cy.
[Ti] Título:HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities.
[So] Source:BMC Infect Dis;17(1):346, 2017 May 16.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Human papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19-65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated. METHODS: HPV detection and typing was carried out using PCR and restriction fragment length polymorphism analysis, respectively. RESULTS: Overall, the HPV prevalence was 72.8%, and it was shown to be age dependent, with a decreasing prevalence until the age of 45 years (p = 0.0018, χ ). Two hundred and fifty-eight women (59.4%) were infected with high-risk HPV, 151 (34.8%) with low-risk HPV, and 25 (5.8%) with HPV types of unknown risk. The most common high-risk HPV type was HPV16 (17.7%), followed by HPV31 (12.9%), HPV58 (7.1%), HPV68 (4.6%), HPV18 (4.1%), and HPV56 (3.7%). Among the women for whom cytology results were available, 268 (77%) were HPV positive, with a sample distribution as follows: 188 (74%) had atypical squamous cells of undetermined significance (ASCUS), 61 (85.9%) had low-grade squamous intraepithelial lesion (L-SIL), and 19 (82.6%) had high-grade squamous intraepithelial lesion (H-SIL). HPV16 was the most common type among women affected by L-SIL (19.7%) and H-SIL (15.8%), with HPV31 being the most common type in women affected by ASCUS (16.5%). CONCLUSIONS: The present study provides the first epidemiological data related to HPV prevalence and type distribution in Cypriot women with cytological abnormalities.
[Mh] Termos MeSH primário: Papillomaviridae/genética
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/epidemiologia
[Mh] Termos MeSH secundário: Adulto
Distribuição por Idade
Idoso
Neoplasia Intraepitelial Cervical/epidemiologia
Neoplasia Intraepitelial Cervical/virologia
Chipre
Feminino
Papillomavirus Humano 16/genética
Papillomavirus Humano 16/isolamento & purificação
Papillomavirus Humano 16/patogenicidade
Seres Humanos
Meia-Idade
Teste de Papanicolaou
Papillomaviridae/patogenicidade
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Neoplasias do Colo do Útero/epidemiologia
Neoplasias do Colo do Útero/virologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2439-0


  8 / 1027 MEDLINE  
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[PMID]:28302094
[Au] Autor:Zannetos S; Zachariadou T; Zachariades A; Georgiou A; Talias MA
[Ad] Endereço:DG European Programmes, Coordination and Development, 29 Byron Avenue, 1096, Nicosia, Cyprus. zannetos@gmail.com.
[Ti] Título:The economic burden of adult asthma in Cyprus; a prevalence-based cost of illness study.
[So] Source:BMC Public Health;17(1):262, 2017 Mar 16.
[Is] ISSN:1471-2458
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Asthma is one of the main non-infectious diseases of the respiratory system with substantial economic burden worldwide. The objective of this study was to estimate the economic burden of adult asthma in Cyprus during 2015. METHODS: A retrospective probabilistic prevalence-based cost of illness model was developed to calculate the economic burden of asthma including direct and indirect costs. The bottom-up approach (person-based data) was used for the calculation of direct costs while for the calculation of indirect costs the approach of human capital was employed. In addition, bootstrapped sensitivity analysis with 1000 bootstrap simulations was performed in order to calculate a 95% Confidence Interval (CI). RESULTS: Mean patient cost of asthma in Cyprus in 2015 was estimated at €579.64 (95% CI: €376.90-€813.68). Direct costs accounted for 82.08% of the overall expenses, €475.75 per patient (95% CI: €296.94-€697.69). Indirect costs of €103.89 (95% CI: €49.59-€181.46) accounted for 17.92% of the overall expenses. CONCLUSION: This was the first study in Cyprus, which used bootstrapped prevalence-based cost of illness model to estimate the cost of asthma. This study confirms that asthma is an expensive disease for the society. In addition, it provides important information and analysis of the economic consequences of asthma to policy makers in order to strengthen surveillance of the disease as well as draft the national health policy accordingly.
[Mh] Termos MeSH primário: Asma/economia
Efeitos Psicossociais da Doença
Custos de Cuidados de Saúde/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adulto
Asma/epidemiologia
Chipre/epidemiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Prevalência
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1186/s12889-017-4184-0


  9 / 1027 MEDLINE  
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[PMID]:28285597
[Au] Autor:Attipa C; Papasouliotis K; Solano-Gallego L; Baneth G; Nachum-Biala Y; Sarvani E; Knowles TG; Mengi S; Morris D; Helps C; Tasker S
[Ad] Endereço:Molecular Diagnostic Unit, Diagnostic Laboratories, Langford Vets and School of Veterinary Sciences, University of Bristol, Langford, UK. attipacy@gmail.com.
[Ti] Título:Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus.
[So] Source:Parasit Vectors;10(1):130, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. METHODS: A cross-sectional, descriptive, multicentre study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus. Real-time quantitative polymerase chain reaction (qPCR) assays were used to detect Mycoplasma haemofelis (Mhf), "Candidatus Mycoplasma haemominutum" (CMhm) and "Candidatus Mycoplasma turicensis" (CMt). The population was tested for four FVBP including Bartonella henselae and Leishmania spp. using qPCR, while conventional PCR assays were used to detect Ehrlichia/Anaplasma spp. and Hepatozoon spp. Serological assays were performed to detect Leishmania infantum antibodies, feline leukaemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies. Statistical analysis was performed to test associations and possible risk factors between variables and infectious agents. RESULTS: Ninety-six (55.2%) of the 174 cats were PCR-positive for at least one infectious agent. Forty-six cats (26.4%) were haemoplasma positive, including 13 (7.5%) for Mhf, 36 (20.7%) for CMhm and 12 (6.9%) for CMt. Sixty-six cats (37.9%) were positive for Hepatozoon spp., while 19 (10.9%) were positive for B. henselae, four (2.3%) for Leishmania spp. and one (0.6%) for Ehrlichia/Anaplasma spp. Sequencing revealed the presence of Hepatozoon felis, L. infantum and Anaplasma platys. Of the 164 cats that underwent retroviral serology, 10 (6.1%) were FeLV-positive and 31 (18.9%) were FIV-positive, while L. infantum serology was positive in 7 (4.4%) of the 160 cats tested. Multivariable logistic regression revealed significant associations for various infectious agents including L. infantum with each of Hepatozoon spp. and CMt infection. CONCLUSIONS: A high prevalence of infectious agents was found in cats from Cyprus with Mhf, CMhm, CMt, L. infantum, B. henselae, H. felis, A. platys, FeLV and FIV infections reported for the first time. The significant associations between different pathogens provide a better understanding of similarities in the epidemiology of these pathogens and interactions between them.
[Mh] Termos MeSH primário: Infecções Bacterianas/veterinária
Doenças do Gato/epidemiologia
Infecções por Mycoplasma/veterinária
Infecções Protozoárias em Animais/epidemiologia
Infecções por Retroviridae/veterinária
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma/isolamento & purificação
Anaplasmose/epidemiologia
Anaplasmose/microbiologia
Animais
Infecções Bacterianas/epidemiologia
Infecções Bacterianas/microbiologia
Doenças do Gato/microbiologia
Doenças do Gato/parasitologia
Doenças do Gato/virologia
Gatos
Coccidiose/epidemiologia
Coccidiose/veterinária
Estudos Transversais
Chipre/epidemiologia
Ehrlichia/genética
Ehrlichia/isolamento & purificação
Ehrlichiose/epidemiologia
Ehrlichiose/microbiologia
Ehrlichiose/veterinária
Análise Fatorial
Feminino
Leishmania infantum/genética
Leishmania infantum/isolamento & purificação
Leishmaniose Visceral/epidemiologia
Leishmaniose Visceral/parasitologia
Leishmaniose Visceral/veterinária
Vírus da Leucemia Felina/genética
Vírus da Leucemia Felina/isolamento & purificação
Masculino
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/microbiologia
Prevalência
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Análise de Regressão
Infecções por Retroviridae/epidemiologia
Infecções por Retroviridae/virologia
Medição de Risco
Fatores de Risco
Doenças Transmitidas por Carrapatos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2063-2


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[PMID]:28167596
[Au] Autor:Seyer A; Karasartova D; Ruh E; Güreser AS; Turgal E; Imir T; Taylan-Ozkan A
[Ad] Endereço:Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, Near East University, Nicosia, Cyprus.
[Ti] Título:Epidemiology and Prevalence of spp. in North Cyprus.
[So] Source:Am J Trop Med Hyg;96(5):1164-1170, 2017 May.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AbstractThis study was conducted to investigate the prevalence of spp. and its subtypes (STs) in North Cyprus; and to evaluate the presence of this parasite and its STs with respect to demographic, socioeconomic, and epidemiological factors, as well as gastrointestinal symptoms. Stool samples were collected from 230 volunteers. Each participant also filled out a questionnaire. The samples were examined microscopically by native-Lugol and trichrome methods and further tested by polymerase chain reaction (PCR) and sequencing. Prevalence of spp. infection was found to be 10.5%, 10.5%, and 27.8%, by direct microscopy, trichrome method, and PCR, respectively. No other parasites were detected in the specimens except spp. ( = 2; 0.8%) and ( = 1; 0.4%). The most common STs were ST3 (20; 31.2%), ST2 (18; 28.2%), ST1 (8; 12.5%), and ST4 (7; 11%); whereas other STs were identified as ST6 (3; 4.7%), ST7 (2; 3.2%), and non-ST (6; 9.4%). Presence of spp. and its STs was not significantly related to any of the demographic, socioeconomic, and epidemiological factors. Furthermore, no significant association of spp. and its STs with gastrointestinal symptoms was found. This study is the first investigation of the epidemiology of spp. in North Cyprus. Distribution of spp. and its STs among demographic, socioeconomic, and epidemiological factors showed complete homogeneity. Presence of the parasite and its STs was not significantly related with the gastrointestinal symptoms among symptomatic and asymptomatic individuals. These findings suggest that spp. may be part of the intestinal flora in humans.
[Mh] Termos MeSH primário: Infecções por Blastocystis/epidemiologia
Blastocystis/genética
DNA de Protozoário/genética
Filogenia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Blastocystis/classificação
Blastocystis/isolamento & purificação
Infecções por Blastocystis/diagnóstico
Infecções por Blastocystis/parasitologia
Criança
Chipre/epidemiologia
Fezes/parasitologia
Feminino
Variação Genética
Seres Humanos
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Prevalência
Análise de Sequência de DNA
Sorotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0706



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