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Pesquisa : B01.043.075.189.250.250.200 [Categoria DeCS]
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  1 / 227 MEDLINE  
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PMID:29274312
Autor:Guo Y; Li N; Ortega YR; Zhang L; Roellig DM; Feng Y; Xiao L
Endereço:College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, 510642, China; Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, 30329, USA.
Título:Population genetic characterization of Cyclospora cayetanensis from discrete geographical regions.
Fonte:Exp Parasitol; 184:121-127, 2018 Jan.
ISSN:1090-2449
País de publicação:United States
Idioma:eng
Resumo:Cyclospora cayetanensis is an emerging pathogen that is endemic in developing countries and responsible for many large foodborne cyclosporiasis outbreaks in North America since 1990s. Because of the lack of typing targets, the genetic diversity and population genetics of C. cayetanensis have not been investigated. In this study, we undertook a population genetic analysis of multilocus sequence typing data we recently collected from 64 C. cayetanensis specimens. Despite the extensive genetic heterogeneity in the overall C. cayetanensis population, there were significant intra- and inter-genic linkage disequilibria (LD). A disappearance of LD was observed when only multilocus genotypes were included in the population genetic analysis, indicative of an epidemic nature of C. cayetanensis. Geographical segregation-associated sub-structuring was observed between specimens from China and those from Peru and the United States. The two subpopulations had reduced LD, indicating the likely occurrence of genetic exchange among isolates in endemic areas. Further analyses of specimens from other geographical regions are necessary to fully understand the population genetics of C. cayetanensis.
Tipo de publicação: JOURNAL ARTICLE


  2 / 227 MEDLINE  
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PMID:28876133
Autor:Whitfield Y; Johnson K; Hanson H; Huneault D
Endereço:1 Public Health Ontario, 480 University Avenue, Suite 300, Toronto, Ontario, Canada M5G 1V2 (ORCID: http://orcid.org/000-0002-4361-1640 [Y.W.]).
Título:2015 Outbreak of Cyclosporiasis Linked to the Consumption of Imported Sugar Snap Peas in Ontario, Canada.
Fonte:J Food Prot; 80(10):1666-1669, 2017 Oct.
ISSN:1944-9097
País de publicação:United States
Idioma:eng
Resumo:An outbreak of cyclosporiasis in Ontario, Canada, was investigated in the fall of 2015. Thirty-five confirmed and 10 probable cases were linked to the investigation. Epidemiological and food safety evidence implicated fresh sugar snap peas imported from Guatemala as the source of the outbreak. We describe here the first documented cyclosporiasis outbreak in Canada involving the consumption of sugar snap peas.
Tipo de publicação: JOURNAL ARTICLE


  3 / 227 MEDLINE  
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PMID:28648295
Autor:Caradonna T; Marangi M; Del Chierico F; Ferrari N; Reddel S; Bracaglia G; Normanno G; Putignani L; Giangaspero A
Endereço:Department of Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71121 Foggia, Italy.
Título:Detection and prevalence of protozoan parasites in ready-to-eat packaged salads on sale in Italy.
Fonte:Food Microbiol; 67:67-75, 2017 Oct.
ISSN:1095-9998
País de publicação:England
Idioma:eng
Resumo:To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in 'ready to eat' (RTE) salads on sale in Italy, 648 packages were purchased from industrial and local brands. Nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. Microscopic examination of 864 slides detected Cryptosporidium spp. but also Blastocystis hominis and Dientamoeba fragilis. Molecular tools identified G. duodenalis assemblage A, Cryptosporidium parvum and Cryptosporidium ubiquitum, T. gondii Type I and C. cayetanensis. B. hominis and D. fragilis were also molecularly confirmed. The overall prevalence of each protozoan species was 0.6% for G. duodenalis, 0.8% for T. gondii, 0.9% for Cryptosporidium spp., and 1.3% for C. cayetanensis, while prevalence for B. hominis was 0.5% and for D. fragilis 0.2%. Microscopy and/or molecular tools revealed that 4.2% of the samples were contaminated by at least one protozoan species, and 0.6% of samples presented contamination by two protozoan species, with a number of oocysts ranging from 62 to 554 per g of vegetable matter for T. gondii, and 46 to 1.580 for C. cayetanensis. This is Europe's first large-scale study on the presence of protozoans in packaged salads, and shows that RTE sanitation processes do not guarantee a product free from protozoans of fecal origin.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Protozoan)


  4 / 227 MEDLINE  
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PMID:28590822
Autor:Murphy HR; Lee S; da Silva AJ
Endereço:U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Food and Environmental Microbiology, Laurel, Maryland 20708, USA.
Título:Evaluation of an Improved U.S. Food and Drug Administration Method for the Detection of Cyclospora cayetanensis in Produce Using Real-Time PCR.
Fonte:J Food Prot; 80(7):1133-1144, 2017 Jul.
ISSN:1944-9097
País de publicação:United States
Idioma:eng
Resumo:Cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with C. cayetanensis oocysts. In the United States, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro and raspberries. An improved method was developed for identification of C. cayetanensis in produce at the U.S. Food and Drug Administration. The method relies on a 0.1% Alconox produce wash solution for efficient recovery of oocysts, a commercial kit for DNA template preparation, and an optimized TaqMan real-time PCR assay with an internal amplification control for molecular detection of the parasite. A single laboratory validation study was performed to assess the method's performance and compare the optimized TaqMan real-time PCR assay and a reference nested PCR assay by examining 128 samples. The samples consisted of 25 g of cilantro or 50 g of raspberries seeded with 0, 5, 10, or 200 C. cayetanensis oocysts. Detection rates for cilantro seeded with 5 and 10 oocysts were 50.0 and 87.5%, respectively, with the real-time PCR assay and 43.7 and 94.8%, respectively, with the nested PCR assay. Detection rates for raspberries seeded with 5 and 10 oocysts were 25.0 and 75.0%, respectively, with the real-time PCR assay and 18.8 and 68.8%, respectively, with the nested PCR assay. All unseeded samples were negative, and all samples seeded with 200 oocysts were positive. Detection rates using the two PCR methods were statistically similar, but the real-time PCR assay is less laborious and less prone to amplicon contamination and allows monitoring of amplification and analysis of results, making it more attractive to diagnostic testing laboratories. The improved sample preparation steps and the TaqMan real-time PCR assay provide a robust, streamlined, and rapid analytical procedure for surveillance, outbreak response, and regulatory testing of foods for detection of C. cayetanensis.
Tipo de publicação: JOURNAL ARTICLE


  5 / 227 MEDLINE  
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PMID:28506035
Autor:Sim S; Won J; Kim JW; Kim K; Park WY; Yu JR
Endereço:Department of Environmental and Tropical Medicine & International Healthcare Research Institute, Konkuk University School of Medicine, Seoul 05029, Korea.
Título:Simultaneous Molecular Detection of and from Raw Vegetables in Korea.
Fonte:Korean J Parasitol; 55(2):137-142, 2017 Apr.
ISSN:1738-0006
País de publicação:Korea (South)
Idioma:eng
Resumo:and are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with , but no data are available regarding the sources of infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, and were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, was isolated from all 6 kinds of vegetables, whereas was detected in 4 kinds of vegetables (except perilla leaves and chives). (17.8%) and (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. was detected all year long; however, was detected only from October to January. In 2 samples (sprout and blueberry), both and were detected. Further investigations using I restriction enzyme fragmentation and nested PCR confirmed and , respectively. In conclusion, we detected in vegetables for the first time in Korea. This suggests that screening should be employed to prevent these protozoal infections in Korea.
Tipo de publicação: JOURNAL ARTICLE


  6 / 227 MEDLINE  
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PMID:28483729
Autor:Karaman Ü; Kolören Z; Seferoglu O; Ayaz E; Demirel E
Endereço:Ordu Üniversitesi Tip Fakültesi, Tibbi Parazitoloji Anabilim Dali, Ordu, Türkiye. ulkukaraman44@hotmail.com.
Título:Presence of Parasites in Environmental Waters in Samsun and Its Districts.
Fonte:Turkiye Parazitol Derg; 41(1):19-21, 2017 Mar.
ISSN:2146-3077
País de publicação:Turkey
Idioma:eng
Resumo:OBJECTIVE: The aim of this study was to detect the presence of parasites in environmental waters in Samsun and its districts. METHODS: At the center of Samsun, 13 stations were determined. The research was performed between March 2012 and February 2013, and every month, water samples were collected on the dates stated. The samples were stained with Kinyoun acid-fast, modified trichrome, and trichrome dyes after examining with the direct bond. The preparations were evaluated in terms of parasitologic under a light microscope. RESULTS: Totally, 180 of 228 water samples analyzed were from streams; of these, 48 were drinking water samples. The following were found: 142 Giardia spp., 132 Cryptosporidium spp., 56 Cyclospora spp., 38 microsporidia, 47 Blastocystis spp., 38 Entamoeba coli cysts, 18 Dientamoeba, 9 Chilomastix, 9 Strongyloides spp., and 6 hookworms. CONCLUSION: The widespread use of animal husbandry and agriculture in the region and the use of stream surroundings as a grazing area increase the presence of some determined protozoa during a certain period. Parasitological studies in humans and animals in the region should be conducted, and control programs should be applied.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Coloring Agents)


  7 / 227 MEDLINE  
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Texto completo SciELO Chile
PMID:28394980
Autor:Weitzel T; Vollrath V; Porte L
Endereço:Universidad del Desarrollo, Santiago, Chile.
Título:[Cyclospora cayetanensis].
Título:Cyclospora cayetanensis..
Fonte:Rev Chilena Infectol; 34(1):45-46, 2017 02.
ISSN:0717-6341
País de publicação:Chile
Idioma:spa
Tipo de publicação: JOURNAL ARTICLE


  8 / 227 MEDLINE  
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PMID:28168979
Autor:Ghozzi K; Marangi M; Papini R; Lahmar I; Challouf R; Houas N; Ben Dhiab R; Normanno G; Babba H; Giangaspero A
Endereço:Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire (code LR12ES08), Faculté de Pharmacie de Monastir, Université de Monastir, Monastir, Tunisia; Laboratoire de Biodiversité et de Biotechnologie Marine, Institut National des Sciences et Technologies de la Mer, Monastir, Tunisia.
Título:First report of Tunisian coastal water contamination by protozoan parasites using mollusk bivalves as biological indicators.
Fonte:Mar Pollut Bull; 117(1-2):197-202, 2017 Apr 15.
ISSN:1879-3363
País de publicação:England
Idioma:eng
Resumo:In order to establish seawater contamination by emerging protozoan parasites, we used qPCR to molecularly characterize and evaluate the parasitic burden of Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii, and Cyclospora cayetanensis in 1255 wild bivalve mollusks collected along the Tunisian coasts. T. gondii, G. duodenalis and C. cayetanensis were detected in 6.9% (99% CI=1.6-12.2%) pools of Ruditapes decussatus. None of the samples were found positive to Cryptosporidium spp.; 6.6% pools of R. decussatus were positive for T. gondii Type I, 1.6% for G. duodenalis assemblage A, and 1.6% for the association T. gondii Type I/C. cayetanensis/G. duodenalis assemblage A. R. decussatus harbored up to 77500 oocysts/sample of T. gondii, up to 395 cysts/sample of G. duodenalis, and 526 oocysts/sample of C. cayetanensis. These results provide the first evidence that the Tunisian coasts are contaminated by zoonotic protozoan parasites that can constitute a direct or indirect risk for human health.
Tipo de publicação: JOURNAL ARTICLE


  9 / 227 MEDLINE  
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PMID:27905288
Autor:Tas Cengiz Z; Beyhan YE; Yilmaz H
Endereço:Yüzüncü Yil Üniversitesi Tip Fakültesi, Parazitoloji Anabilim Dali, Van, Türkiye. ztas72@yahoo.com.
Título:Cyclospora cayetanensis, Opportunistic Protozoan Parasite, in Van Province, Turkey: A Report of Seven Cases.
Fonte:Turkiye Parazitol Derg; 40(3):166-168, 2016 Sep.
ISSN:2146-3077
País de publicação:Turkey
Idioma:eng
Resumo:The aim of this study is to report seven Cyclospora cayetanensis (C.cayetanensis) cases determined in Yuzuncu Yil University Medical Faculty Parasitology Laboratory, Van province, Turkey. In the study native-Lugol, formalin-ethyl acetate and modified acid-fast staining methods were performed to stool samples of the patients sent from outpatient clinics of the hospital. All of the patients infected with C. cayetanensis were older than 15 years. In our cases there were not a history of international travel. Only one of our seven cases was immunosuppressed. Trimethoprim-sulfamethoxazole (160/800 mg) was administered for the treatment of the patient. In conclusion, it was understood that the physicians should consider cyclosporiosis in the patients with diarrhea and abdominal pain.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)


  10 / 227 MEDLINE  
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PMID:27899155
Autor:Cinar HN; Qvarnstrom Y; Wei-Pridgeon Y; Li W; Nascimento FS; Arrowood MJ; Murphy HR; Jang A; Kim E; Kim R; da Silva A; Gopinath GR
Endereço:Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD, USA. hediye.cinar@fda.hhs.gov.
Título:Comparative sequence analysis of Cyclospora cayetanensis apicoplast genomes originating from diverse geographical regions.
Fonte:Parasit Vectors; 9(1):611, 2016 Nov 29.
ISSN:1756-3305
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Cyclospora cayetanensis is an emerging coccidian parasite that causes endemic and epidemic diarrheal disease called cyclosporiasis, and this infection is associated with consumption of contaminated produce or water in developed and developing regions. Food-borne outbreaks of cyclosporiasis have occurred almost every year in the USA since the 1990s. Investigations of these outbreaks are currently hampered due to lack of molecular epidemiological tools for trace back analysis. The apicoplast of C. cayetanensis, a relict non-photosynthetic plastid with an independent genome, provides an attractive target to discover sequence polymorphisms useful as genetic markers for detection and trace back analysis of the parasite. Distinct differences in the apicoplast genomes of C. cayetanensis could be useful in designing advanced molecular methods for rapid detection and, subtyping and geographical source attribution, which would aid outbreak investigations and surveillance studies. METHODS: To obtain the genome sequence of the C. cayetanensis apicoplast, we sequenced the C. cayetanensis genomic DNA extracted from clinical stool samples, assembled and annotated a 34,146 bp-long circular sequence, and used this sequence as a reference genome in this study. We compared the genome and the predicted proteome to the data available from other apicomplexan parasites. To initialize the search for genetic markers, we mapped the raw sequence reads from an additional 11 distinct clinical stool samples originating from Nepal, New York, Texas, and Indonesia to the apicoplast reference genome. RESULTS: We identified several high quality single nucleotide polymorphisms (SNPs) and small insertion/deletions spanning the apicoplast genome supported by extensive sequencing reads data, and a 30 bp sequence repeat at the terminal spacer region in a Nepalese sample. The predicted proteome consists of 29 core apicomplexan peptides found in most of the apicomplexans. Cluster analysis of these C. cayetanensis apicoplast genomes revealed a familiar pattern of tight grouping with Eimeria and Toxoplasma, separated from distant species such as Plasmodium and Babesia. CONCLUSIONS: SNPs and sequence repeats identified in this study may be useful as genetic markers for identification and differentiation of C. cayetanensis isolates found and could facilitate outbreak investigations.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE



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