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Pesquisa : B01.050.500.500.736.715.800.610 [Categoria DeCS]
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  1 / 663 MEDLINE  
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PMID:28395568
Autor:Shin DH; Kim YS; Yoo DS; Kim MJ; Oh CS; Hong JH; Lee E; Chai JY; Seo M
Endereço:Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, 103 Daehak-ro (Yongon-dong), Jongno-gu, Seoul 03080, South Korea. Correspondence should be sent to M. Seo at: bbbenji@naver.com.
Título:A Case of Ectopic Paragonimiasis in a 17th Century Korean Mummy.
Fonte:J Parasitol; 103(4):399-403, 2017 Aug.
ISSN:1937-2345
País de publicação:United States
Idioma:eng
Resumo:Archaeoparasitological studies on fossilized feces obtained from Joseon Dynasty (1392-1910 CE) mummies have provided invaluable data on the patterns of parasitic infection in pre-modern Korean societies. In our recent radiological investigation of a 17th century Joseon mummy discovered in Cheongdo (South Korea), we located a liver mass just below the diaphragm. Anatomical dissection confirmed the presence of a mass of unknown etiology. A subsequent parasitological examination of a sample of the mass revealed a large number of ancient Paragonimus sp. eggs, making the current report the first archaeoparasitological case of liver abscess caused by ectopic paragonimiasis.
Tipo de publicação: CASE REPORTS; HISTORICAL ARTICLE; JOURNAL ARTICLE


  2 / 663 MEDLINE  
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PMID:28027969
Autor:Hernández-Chea R; Jiménez-Rocha AE; Castro R; Blair D; Dolz G
Endereço:Maestría en Enfermedades Tropicales, Posgrado Regional en Ciencias Veterinarias Tropicales, Universidad Nacional, Campus Benjamín Nuñez, Barreal de Heredia, P.O. Box 86, 3000 Heredia, Costa Rica. Electronic address: roderik123animal@gmail.com.
Título:Morphological and molecular characterization of the metacercaria of Paragonimus caliensis, as a separate species from P. mexicanus in Costa Rica.
Fonte:Parasitol Int; 66(2):126-133, 2017 Apr.
ISSN:1873-0329
País de publicação:Netherlands
Idioma:eng
Resumo:The trematode Paragonimus mexicanus is the etiological agent of paragonimiasis, a food-borne zoonotic disease in Latin America. This species, as well as Paragonimus caliensis, have been reported from Costa Rica, but it is not known if the two are synonymous. Two types of Paragonimus metacercariae from freshwater pseudothelphusid crabs from several localities in Costa Rica were recognized by light microscopy. Morphologically, these corresponded to descriptions of P. mexicanus and P. caliensis. Metacercariae of the former species lacked a membrane or cyst and their bodies were yellow in color. Those of P. caliensis were contained in a transparent thin cyst and were pink in color. Morphotypes of metacercariae were determined using scanning electron microscopy (SEM). Based on the number and distribution of papillae in the ventral sucker, three morphotypes were found for P. mexicanus and two for P. caliensis. Analysis of DNA sequences (nuclear ribosomal 28S and ITS2 genes, and partial mitochondrial cox1 gene) confirmed the presence of P. mexicanus and provided the first molecular data for P. caliensis. The two species are phylogenetically distinct from each other and distant from the Asian species. The confirmation of P. caliensis as a separate species from P. mexicanus raises several questions about the ecology, biological diversity, and epidemiology of the genus Paragonimus in Costa Rica.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer)


  3 / 663 MEDLINE  
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PMID:27879464
Autor:Calvopina M; Romero-Alvarez D; Macias R; Sugiyama H
Endereço:Unidad de Parasitología Molecular y Medicina Tropical, Carrera de Medicina, Universidad De Las Américas (UDLA), Quito, Ecuador. manuelcalvopina@gmail.com manuel.calvopina@udla.edu.ec.
Título:Severe Pleuropulmonary Paragonimiasis Caused by Paragonimus mexicanus Treated as Tuberculosis in Ecuador.
Fonte:Am J Trop Med Hyg; 96(1):97-99, 2017 Jan 11.
ISSN:1476-1645
País de publicação:United States
Idioma:eng
Resumo:A 30-year-old male, from a subtropical region of Ecuador, was hospitalized with a 5-year history of persistent cough with rusty brown sputum, chest pain, and progressive dyspnea. The patient underwent thoracic surgery 3 years ago for pleural effusion and subsequently received a 9-month regimen treatment of tuberculosis. However, there was no clinical resolution and symptoms became progressively worse. A chest radiograph and computerized tomography scan showed several small nodules in both lungs. Eggs of Paragonimus spp. were observed in sputum smears, but the smears were negative for acid-fast bacilli. Molecular characterization of eggs by the internal transcribed spacer-2 regions identified them as Paragonimus mexicanus The patient was treated with praziquantel and tested negative parasitologically for 12 months. There was clinical resolution of the cough and expectoration, but dyspnea and chest pain persisted.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:6490C9U457 (Praziquantel)


  4 / 663 MEDLINE  
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PMID:27796563
Autor:Yu S; Zhang X; Chen W; Zheng H; Ai G; Ye N; Wang Y
Endereço:Institute of Tropical Medicine, Third Military Medical University, Chongqing, 400038, China.
Título:Development of an immunodiagnosis method using recombinant PsCP for detection of Paragonimus skrjabini infection in human.
Fonte:Parasitol Res; 116(1):377-385, 2017 Jan.
ISSN:1432-1955
País de publicação:Germany
Idioma:eng
Resumo:Paragonimiasis skrjabini is a kind of zoonosis and prevalent in 16 provinces in China, such as Chongqing, Fujian, Sichuan, and Yunnan. However, sensitive and efficient diagnostic methods for the infection are limited. In order to provide a more convenient and simple method for serologic diagnosis, the recombinant P. skrjabini cysteine protease (PsCP) was expressed, purified, and then used to develop an indirect enzyme-linked immunosorbent assay (ELISA) for detecting anti-PsCP antibodies in human. Given the positive/negative cutoff value as 0.606, the maximum dilution of human sera in which anti-PsCP antibodies could be detected was 1:12,800. In addition, the coefficients of variation (CVs) of inter-assay and intra-assay experiments were both below 10 %. Furthermore, the sensitivity of the PsCP-based ELISA was 95.5 %, and the indirect ELISA displays no cross-reactivity with human antisera against Echinococcus granulosus, Taenia solium, Schistosoma japonicum, and Trichinella spiralis, either. In conclusion, recombinant PsCP was readily produced and used to establish a simple PsCP-based ELISA that provided a highly specific and sensitive method for analysis of clinical samples. Besides, the method can also probably be used to diagnose P. skrjabini infection in animals.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Immune Sera); 0 (Recombinant Proteins)


  5 / 663 MEDLINE  
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PMID:27928089
Autor:Amaro DE; Cowell A; Tuohy MJ; Procop GW; Morhaime J; Reed SL
Endereço:Department of Pathology, University of California San Diego School of Medicine, La Jolla, California.
Título:Cerebral Paragonimiasis Presenting with Sudden Death.
Fonte:Am J Trop Med Hyg; 95(6):1424-1427, 2016 Dec 07.
ISSN:1476-1645
País de publicação:United States
Idioma:eng
Resumo:A 58-year-old Korean-born woman with a history of seizures and psychiatric issues was found dead at home. Autopsy was notable for large, calcified nodules that had nearly replaced her right temporal lobe. Histologic examination revealed the presence of Paragonimus eggs. This case demonstrates a rare manifestation of an aberrantly migrated lung fluke that resulted in epilepsy and sudden death years after the initial infection.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE


  6 / 663 MEDLINE  
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PMID:27562899
Autor:Yoonuan T; Nuamtanong S; Dekumyoy P; Phuphisut O; Adisakwattana P
Endereço:Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Ratchathewi, Bangkok, 10400, Thailand.
Título:Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus.
Fonte:Parasitol Res; 115(12):4457-4470, 2016 Dec.
ISSN:1432-1955
País de publicação:Germany
Idioma:eng
Resumo:Cathepsin L is a cysteine protease belonging to the papain family. In parasitic trematodes, cathepsin L plays essential roles in parasite survival and host-parasite interactions. In this study, cathepsin L of the lung fluke Paragonimus pseudoheterotremus (PpsCatL) was identified and its molecular biological and immunological features characterized. A sequence analysis of PpsCatL showed that the gene encodes a 325-amino-acid protein that is most similar to P. westermani cathepsin L. The in silico three-dimensional structure suggests that PpsCatL is a pro-enzyme that becomes active when the propeptide is cleaved. A recombinant pro-PpsCatL lacking the signal peptide (rPpsCatL), with a molecular weight of 35 kDa, was expressed in E. coli and reacted with P. pseudoheterotremus-infected rat sera. The native protein was detected in crude worm antigens and excretory-secretory products and was localized in the cecum and in the lamellae along the intestinal tract of the adult parasite. Enzymatic activity of rPpsCatL showed that the protein could cleave the fluorogenic substrate Z-Phe-Arg-AMC after autocatalysis but was inhibited with E64. The immunodiagnostic potential of the recombinant protein was evaluated with an enzyme-linked immunosorbent assay (ELISA) and suggested that rPpsCatL can detect paragonimiasis with high sensitivity and specificity (100 and 95.6 %, respectively). This supports the further development of an rPpsCatL-ELISA as an immunodiagnostic tool.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antigens, Helminth); 0 (Helminth Proteins); EC 3.4.22.15 (Cathepsin L)


  7 / 663 MEDLINE  
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PMID:27172340
Autor:Broman MM; Miller MA
Título:Pathology in Practice.
Fonte:J Am Vet Med Assoc; 248(11):1253-5, 2016 Jun 01.
ISSN:1943-569X
País de publicação:United States
Idioma:eng
Tipo de publicação: JOURNAL ARTICLE


  8 / 663 MEDLINE  
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PMID:26740365
Autor:Tantrawatpan C; Saijuntha W; Manochantr S; Kheolamai P; Thanchomnang T; Sadaow L; Intapan PM; Maleewong W
Endereço:Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, Thailand Center of Excellence in Stem Cell Research, Thammasat University, Pathumthani, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University
Título:A singleplex real-time fluorescence resonance energy transfer PCR with melting curve analysis for the differential detection of Paragonimus heterotremus, Echinostoma malayanum and Fasciola gigantica eggs in faeces.
Fonte:Trans R Soc Trop Med Hyg; 110(1):74-83, 2016 Jan.
ISSN:1878-3503
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Because the eggs of Paragonimus, Echinostoma and Fasciola are very similar in size and shape, it is difficult to distinguish and accurately identify species by the morphology of their eggs, which is a standard diagnostic method. METHODS: In this study, a novel assay combining a real-time fluorescence resonance energy transfer PCR and melting curve analysis using one set of primers and fluorophore-labelled hybridization probes specific for the 28S rDNA region was developed for the molecular detection of Paragonimus heterotremus, Echinostoma malayanum and Fasciola gigantica eggs. RESULTS: This assay could detect and distinguish P. heterotremus, E. malayanum and F. gigantica DNA with the distinct melting temperature (Tm) values of 57.99±0.08, 62.12±0.15 and 74.10±0.18, respectively. The assay can also be used to detect and distinguish DNA from P. bangkokensis, P. harinasutai, P. machorchis, E. revolutum, Hypodereum conoideum and F. hepatica, which have different Tm values. The sensitivity of this assay enabled the detection of one egg of P. heterotremus, E. malayanum or F. gigantica per 100 mg of faeces. In addition, the specificity testing showed no fluorescence signal for other parasites. CONCLUSIONS: Due to the sensitivity and specificity of our assay in detecting P. heterotremus, E. malayanum and F. gigantica, our method could be used to accurately diagnose these three medically important parasitic groups and has potential implications for molecular epidemiological investigations of human and/or animal infections.
Tipo de publicação: JOURNAL ARTICLE


  9 / 663 MEDLINE  
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PMID:26740364
Autor:Saijuntha W; Tantrawatpan C; Jarilla BR; Agatsuma T; Andrews RH; Petney TN
Endereço:Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Maha Sarakham 44150, Thailand.
Título:Intron sequence of the taurocyamine kinase gene as a marker to investigate genetic variation of Paragonimus species in Japan and the origins of triploidy in P. westermani.
Fonte:Trans R Soc Trop Med Hyg; 110(1):67-73, 2016 Jan.
ISSN:1878-3503
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Paragonimiasis is a foodborne parasitic infection caused by lung flukes of the genus Paragonimus. Several species of Paragonimus are endemic in Japan: P. westermani (diploid and triploid) P. miyazakii, P. ohirai and P. iloktsuenensis. The taxonomic status and genetic variability of these lung flukes remains poorly understood. METHODS: The second intron of domain 1 of the taurocyamine kinase gene (TKD1int2) region was used to explore genetic variation and differentiation of diploid and triploid P. westermani, as well as P. miyazakii, P. ohirai and P. iloktsuenensis originating from Japan. RESULTS: We found high levels of intraspecific variation in P. westermani, but only low levels of variation within the other species studied. Haplotype network and phylogenetic tree analyses demonstrated the sister-group relationship of P. ohirai and P. iloktsuenensis and the phylogenetically distant relationship of P. westermani with the other species. All individuals except for triploid P. westermani were homozygous. Each triploid contained at least one allele similar to that seen in most diploids from Chiba and one allele resembling that seen in diploids from Oita. One triploid contained three different sequences. CONCLUSIONS: Our findings suggested that the TKD1int2 region is a suitable marker for use in studying the genetic variation and phylogenetics of Paragonimus species, as well as providing clues to the origins of triploidy in P. westermani.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Helminth); 0 (Genetic Markers); EC 2.7.3.- (Phosphotransferases (Nitrogenous Group Acceptor)); EC 2.7.3.4 (taurocyamine kinase)


  10 / 663 MEDLINE  
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PMID:26740357
Autor:Blair D; Nawa Y; Mitreva M; Doanh PN
Endereço:College of Marine and Environmental Sciences, James Cook University, Townsville, Qld 4811, Australia david.blair@jcu.edu.au.
Título:Gene diversity and genetic variation in lung flukes (genus Paragonimus).
Fonte:Trans R Soc Trop Med Hyg; 110(1):6-12, 2016 Jan.
ISSN:1878-3503
País de publicação:England
Idioma:eng
Resumo:Paragonimiasis caused by lung flukes (genus Paragonimus) is a neglected disease occurring in Asia, Africa and the Americas. The genus is species-rich, ancient and widespread. Genetic diversity is likely to be considerable, but investigation of this remains confined to a few populations of a few species. In recent years, studies of genetic diversity have moved from isoenzyme analysis to molecular phylogenetic analysis based on selected DNA sequences. The former offered better resolution of questions relating to allelic diversity and gene flow, whereas the latter is more suitable for questions relating to molecular taxonomy and phylogeny. A picture is emerging of a highly diverse taxon of parasites, with the greatest diversity found in eastern and southern Asia where ongoing speciation might be indicated by the presence of several species complexes. Diversity of lung flukes in Africa and the Americas is very poorly sampled. Functional molecules that might be of value for immunodiagnosis, or as targets for medical intervention, are of great interest. Characterisation of these from Paragonimus species has been ongoing for a number of years. However, the imminent release of genomic and transcriptomic data for several species of Paragonimus will dramatically increase the rate of discovery of such molecules, and illuminate their diversity within and between species.
Tipo de publicação: JOURNAL ARTICLE; REVIEW
Nome de substância:0 (DNA, Helminth)



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