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  1 / 100 MEDLINE  
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PMID:28348084
Autor:Martínez-Sernández V; Mezo M; González-Warleta M; Perteguer MJ; Gárate T; Romarís F; Ubeira FM
Endereço:From the Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Título:Delineating distinct heme-scavenging and -binding functions of domains in MF6p/helminth defense molecule (HDM) proteins from parasitic flatworms.
Fonte:J Biol Chem; 292(21):8667-8682, 2017 May 26.
ISSN:1083-351X
País de publicação:United States
Idioma:eng
Resumo:MF6p/FhHDM-1 is a small protein secreted by the parasitic flatworm (trematode) that belongs to a broad family of heme-binding proteins (MF6p/helminth defense molecules (HDMs)). MF6p/HDMs are of interest for understanding heme homeostasis in trematodes and as potential targets for the development of new flukicides. Moreover, interest in these molecules has also increased because of their immunomodulatory properties. Here we have extended our previous findings on the mechanism of MF6p/HDM-heme interactions and mapped the protein regions required for heme binding and for other biological functions. Our data revealed that MF6p/FhHDM-1 forms high-molecular-weight complexes when associated with heme and that these complexes are reorganized by a stacking procedure to form fibril-like and granular nanostructures. Furthermore, we showed that MF6p/FhHDM-1 is a transitory heme-binding protein as protein·heme complexes can be disrupted by contact with an apoprotein ( apomyoglobin) with higher affinity for heme. We also demonstrated that (i) the heme-binding region is located in the MF6p/FhHDM-1 C-terminal moiety, which also inhibits the peroxidase-like activity of heme, and (ii) MF6p/HDMs from other trematodes, such as and , also bind heme. Finally, we observed that the N-terminal, but not the C-terminal, moiety of MF6p/HDMs has a predicted structural analogy with cell-penetrating peptides and that both the entire protein and the peptide corresponding to the N-terminal moiety of MF6p/FhHDM-1 interact with cell membranes in hemin-preconditioned erythrocytes. Our findings suggest that MF6p/HDMs can transport heme in trematodes and thereby shield the parasite from the harmful effects of heme.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Carrier Proteins); 0 (Helminth Proteins); 42VZT0U6YR (Heme)


  2 / 100 MEDLINE  
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PMID:28127717
Autor:Kim TI; Oh SR; Dai F; Yang HJ; Ha SD; Hong SJ
Endereço:Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, Seoul, 06974, South Korea.
Título:Inactivation of Paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs.
Fonte:Parasitol Res; 116(3):1003-1006, 2017 Mar.
ISSN:1432-1955
País de publicação:Germany
Idioma:eng
Resumo:Soy sauce-marinated freshwater crabs (Eriocheir japonicus) are a source of human paragonimiasis. The viability of Paragonimus westermani metacercariae (PwMc) in marinated crabs was investigated in an experimental setting. The PwMc collected from freshwater crayfish were inoculated into freshwater crabs, which were then frozen or marinated in soy sauce. All PwMc in the freshwater crabs were inactivated after freezing for 48 h at -20 °C and after freezing for 12 h at -40 °C. After marinating for 32 days, the survival rate of PwMc in 5% NaCl soy sauce was 50%, in 7.5% NaCl soy sauce it was 33.3%, and in 10.0% NaCl soy sauce it was 31.3%. When marinated for 64 days, all PwMc were inactivated in all experimental groups. These results revealed that freezing and soy sauce marination were detrimental to the survival of PwMc in freshwater crabs. Specifically, freezing crabs for more than 48 h or soaking them in soy sauce containing at least 5.0% NaCl for 64 days can inactivate PwMc. These results can inform the production of the traditional Korean soy sauce-marinated freshwater crabs known as gejang.
Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE
Nome de substância:0 (Food Preservatives); 451W47IQ8X (Sodium Chloride)


  3 / 100 MEDLINE  
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PMID:27725555
Autor:Nureki SI; Ishii K; Fujisaki H; Torigoe M; Maeshima K; Shibata H; Miyazaki E; Kadota JI
Endereço:Department of Respiratory Medicine and Infectious Diseases, Oita University Faculty of Medicine, Japan.
Título:Familial Mediterranean Fever with Rheumatoid Arthritis Complicated by Pulmonary Paragonimiasis.
Fonte:Intern Med; 55(19):2889-2892, 2016.
ISSN:1349-7235
País de publicação:Japan
Idioma:eng
Resumo:A 42-year-old woman presented with an intermittent fever and chest and back pain, and an abnormal chest shadow was detected. She was diagnosed with paragonimiasis caused by Paragonimus westermani. Praziquantel therapy improved the abnormal chest shadow, but did not relieve her symptoms. She was also diagnosed with familial Mediterranean fever (FMF), and colchicine therapy resolved her symptoms. She subsequently developed arthralgia and morning stiffness in her hands. We also diagnosed the patient with rheumatoid arthritis (RA), and corticosteroid and salazosulfapyridine therapy improved her symptoms. The existence of paragonimiasis complicated the diagnosis of FMF. The coexistence of FMF and RA is very rare, but does exist.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:0 (Adrenal Cortex Hormones); 3XC8GUZ6CB (Sulfasalazine); 6490C9U457 (Praziquantel); SML2Y3J35T (Colchicine)


  4 / 100 MEDLINE  
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PMID:27644890
Autor:Yoshida A; Matsuo K; Moribe J; Tanaka R; Kikuchi T; Nagayasu E; Misawa N; Maruyama H
Endereço:Division of Parasitology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Japan.
Título:Venison, another source of Paragonimus westermani infection.
Fonte:Parasitol Int; 65(6 Pt A):607-612, 2016 Dec.
ISSN:1873-0329
País de publicação:Netherlands
Idioma:eng
Resumo:Paragonimiasis is a typical food-borne parasitic disease, endemic in most parts of Asia, with sporadic case reports from American and African countries. The major source of infection is undercooked freshwater crab or crayfish, though consumption of wild boar meat is also responsible for the infection in Japan, because wild boar is a paratenic host for Paragonimus westermani. Recently, living juveniles of P. westermani were isolated from muscle of a sika deer, Cervus nippon, in Japan, raising the possibility that venison has been another source of infection. In order to clarify the potential contribution of venison consumption to the occurrence of paragonimiasis, we analysed dietary histories of those paragonimiasis patients in whose diagnoses we were involved between 2001 and 2015. Among 380 patients, freshwater crab had been consumed by 208 patients, wild boar meat by 190, and wild deer meat by 76 patients before the onset of the disease. Overall contribution of wild deer meat was estimated to be 6.8% to 20.0%, although in Oita and Gifu Prefectures, where a substantial proportion of patients had consumed raw venison, the contribution of venison consumption was much higher (27.5 to 62.1% and 42.1 to 78.9% in Oita and Gifu Prefectures, respectively). We demonstrated P. westermani-specific antibodies in the sera of 4 out of 160 sika deer from Gifu Prefecture, strongly suggesting that these deer were infected with P. westermani.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antibodies, Protozoan); 0 (Antigens, Protozoan)


  5 / 100 MEDLINE  
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PMID:27498617
Autor:Itoh N; Tsukahara M; Yamasaki H; Morishima Y; Sugiyama H; Kurai H
Endereço:Division of Infectious Diseases, Shizuoka Cancer Center Hospital, Sunto-gun, Shizuoka, Japan. Electronic address: itohnaoya0925@ybb.ne.jp.
Título:Paragonimus westermani infection mimicking recurrent lung cancer: A case report.
Fonte:J Infect Chemother; 22(12):815-818, 2016 Dec.
ISSN:1437-7780
País de publicação:Netherlands
Idioma:eng
Resumo:Herein, we report a case of Paragonimus westermani infection, which required differentiation from recurrent lung cancer. A 66-year old Japanese man with a history of lung cancer who had undergone a lobectomy was referred to our clinic for treatment of cough, sputum, dyspnea, and a right pulmonary nodule. He had previously eaten seafood he visited China. P. westermani infection was confirmed by the presence of antibody against P. westermani antigen in the patient's serum and eggs in his sputum. Eventually, molecular identification by PCR-restriction fragment length polymorphism analysis and sequencing confirmed that the patient was infected with triploid forms of P. westermani.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE


  6 / 100 MEDLINE  
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PMID:27440217
Autor:Doanh NP; Tu AL; Bui TD; Loan TH; Nonaka N; Horii Y; Blair D; Nawa Y
Endereço:Department of Parasitology,Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology,Hanoi,Vietnam.
Título:Molecular and morphological variation of Paragonimus westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province.
Fonte:Parasitology; 143(12):1639-46, 2016 Oct.
ISSN:1469-8161
País de publicação:England
Idioma:eng
Resumo:Paragonimus westermani is one of the most medically important lung flukes and is widely distributed in Asia. It exhibits considerable variation in morphological, genetic and biological features. In central provinces of Vietnam, a high prevalence of metacercariae of this species has been reported from the crab intermediate host, Vietopotamon aluoiense. In this study, we detected P. westermani metacercariae in two additional crab hosts, Donopotamon haii in Quang Tri Province, central Vietnam and Indochinamon tannanti in Yen Bai Province in the north. The latter is a new locality for P. westermani in a northern region of Vietnam where P. heterotremus is the only species currently known to cause human paragonimiasis. Paragonimus westermani metacercariae found in Vietnam showed considerable morphological variation but slight genetic variation based on DNA sequences from the nuclear ribosomal ITS2 region and the mitochondrial 16S gene. Co-infection of the same individual crabs with P. westermani and P. heterotremus and/or some other Paragonimus species was found frequently, suggesting potential for co-infection in humans. The findings of the present study emphasize the need for highly specific molecular and immunodiagnostic methods to differentially diagnose between P. westermani and P. heterotremus infections.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Helminth); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S)


  7 / 100 MEDLINE  
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PMID:27082624
Autor:Luo J; Wang MY; Liu D; Zhu H; Yang S; Liang BM; Liang ZA
Endereço:From the Department of Respiratory Diseases (JL, M-YW, HZ, SY, B-ML, Z-AL); and Critical Care Medicine (DL), West China School of Medicine and West China Hospital, Sichuan University, Chengdu, China.
Título:Pulmonary Paragonimiasis Mimicking Tuberculous Pleuritis: A Case Report.
Fonte:Medicine (Baltimore); 95(15):e3436, 2016 Apr.
ISSN:1536-5964
País de publicação:United States
Idioma:eng
Resumo:Pulmonary paragonimiasis is a food-borne zoonosis with a wide variety of radiologic findings, which sometimes can be confused with tuberculosis and carcinoma. Therefore, differential diagnosis is always warranted. A 43-year-old male farmer, with productive cough, blood-tinged sputum and chest pain, as well as patchy consolidation and pleural effusions in chest computer tomography, was misdiagnosed of community-acquired pneumonia and tuberculosis. Complete blood cell count, sputum smear and culture, chest computer tomography, thoracoscopy, and biopsy. The diagnosis of pulmonary paragonimiasis was established due to the finding of Charcot-Leyden crystals in the pleural necrosis, and antibodies against Paragonimus westermani in enzyme-linked immunosorbent assay. Paragonimiasis should be considered as a possibility in the differential diagnosis of tuberculosis. Thoracoscopy is an effective and valuable technology that can help make an accurate diagnosis.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE


  8 / 100 MEDLINE  
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PMID:26935371
Autor:Akaba T; Takeyama K; Toriyama M; Kubo A; Mizobuchi R; Yamada T; Tagaya E; Kondo M; Sakai S; Tamaoki J
Endereço:The First Department of Medicine, Tokyo Women's Medical University, Japan.
Título:Pulmonary Paragonimiasis: The Detection of a Worm Migration Track as a Diagnostic Clue for Uncertain Eosinophilic Pleural Effusion.
Fonte:Intern Med; 55(5):503-6, 2016.
ISSN:1349-7235
País de publicação:Japan
Idioma:eng
Resumo:A 38-year-old woman with sustained right chest pain was referred to our hospital. She showed pleural effusion and peripheral blood eosinophilia. Thoracentesis revealed eosinophilic pleural effusion in which the smear, culture and cytological examinations were all negative. Although she had no notable dietary history, chest CT revealed linear opacities, which suggested the migration tracks of paragonimiasis. The diagnosis was confirmed using enzyme-linked immunosorbent assays, which showed elevated Paragonimus westermani and Paragonimus miyazakii antibody levels. After the initiation of praziquantel therapy, all clinical findings were promptly improved. The detection of a migration track may therefore be useful in the diagnosis of paragonimiasis.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:0 (Anthelmintics); 6490C9U457 (Praziquantel)


  9 / 100 MEDLINE  
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PMID:26740364
Autor:Saijuntha W; Tantrawatpan C; Jarilla BR; Agatsuma T; Andrews RH; Petney TN
Endereço:Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Maha Sarakham 44150, Thailand.
Título:Intron sequence of the taurocyamine kinase gene as a marker to investigate genetic variation of Paragonimus species in Japan and the origins of triploidy in P. westermani.
Fonte:Trans R Soc Trop Med Hyg; 110(1):67-73, 2016 Jan.
ISSN:1878-3503
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Paragonimiasis is a foodborne parasitic infection caused by lung flukes of the genus Paragonimus. Several species of Paragonimus are endemic in Japan: P. westermani (diploid and triploid) P. miyazakii, P. ohirai and P. iloktsuenensis. The taxonomic status and genetic variability of these lung flukes remains poorly understood. METHODS: The second intron of domain 1 of the taurocyamine kinase gene (TKD1int2) region was used to explore genetic variation and differentiation of diploid and triploid P. westermani, as well as P. miyazakii, P. ohirai and P. iloktsuenensis originating from Japan. RESULTS: We found high levels of intraspecific variation in P. westermani, but only low levels of variation within the other species studied. Haplotype network and phylogenetic tree analyses demonstrated the sister-group relationship of P. ohirai and P. iloktsuenensis and the phylogenetically distant relationship of P. westermani with the other species. All individuals except for triploid P. westermani were homozygous. Each triploid contained at least one allele similar to that seen in most diploids from Chiba and one allele resembling that seen in diploids from Oita. One triploid contained three different sequences. CONCLUSIONS: Our findings suggested that the TKD1int2 region is a suitable marker for use in studying the genetic variation and phylogenetics of Paragonimus species, as well as providing clues to the origins of triploidy in P. westermani.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Helminth); 0 (Genetic Markers); EC 2.7.3.- (Phosphotransferases (Nitrogenous Group Acceptor)); EC 2.7.3.4 (taurocyamine kinase)


  10 / 100 MEDLINE  
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PMID:26420425
Autor:Gao H; Xiao D; Song L; Zhang W; Shen S; Yin X; Wang J; Ke X; Yu C; Zhang J
Endereço:Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, People's Republic of China.
Título:Assessment of the diagnostic efficacy of enolase as an indication of active infection of Schistosoma japonicum.
Fonte:Parasitol Res; 115(1):151-64, 2016 Jan.
ISSN:1432-1955
País de publicação:Germany
Idioma:eng
Resumo:Schistosomiasis is a common zoonoses affecting humans. The atypical clinical symptoms, low morbidity, and low degree of infection impede diagnosis and assessment of epidemics. Detecting circulating antigens from adult worms in patients' body fluids should be diagnostically superior to examining eggs in feces. Herein, the excretory-secretory proteins of adult worms were analyzed by using 2-D protein electrophoresis and mass spectrometry. The Schistosoma japonicum enolase (Sj enolase) was identified as the most abundant excretory-secretory antigen. Purified recombinant Sj enolase was prepared, and specific monoclonal and polyclonal antibodies were raised against it. A sandwich enzyme-linked immunoassay (sandwich ELISA) was established that used the monoclonal antibody as a capture antibody and the polyclonal antibody as a detection antibody. The linear detection range was 0.7-1000 ng/ml (minimum 700 pg/ml). Sj enolase could be detected in the sera of infected rabbits and disappeared rapidly postpraziquantel treatment. The sensitivity and specificity of this sandwich ELISA to detect field serum samples of schistosomiasis were 84.61 and 95.83 %, respectively. The cross-reaction rates for clonorchiasis and paragonimiasis were 3.33 and 5 %, respectively. This ELISA assay was used to test 45 matching sera of schistosomiasis patients before treatment and at 3, 6, 9, and 12 months posttreatment. Among the sera, 88.89 % were positive before treatment. At 3, 6, 9, and 12 months postpraziquantel treatment, 93.33, 97.78, 100, and 100 % tested negative, respectively. Therefore, Sj enolase can be used to indicate active Schistosoma infection, and detecting serum Sj enolase is important for diagnosis and evaluating treatment effect.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Antibodies, Helminth); 0 (Antibodies, Monoclonal); 0 (Antigens, Helminth); EC 4.2.1.11 (Phosphopyruvate Hydratase)



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