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  1 / 87 MEDLINE  
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PMID:28945741
Autor:Laurella LC; Cerny N; Bivona AE; Sánchez Alberti A; Giberti G; Malchiodi EL; Martino VS; Catalan CA; Alonso MR; Cazorla SI; Sülsen VP
Endereço:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Farmacognosia, Buenos Aires, Argentina.
Título:Assessment of sesquiterpene lactones isolated from Mikania plants species for their potential efficacy against Trypanosoma cruzi and Leishmania sp.
Fonte:PLoS Negl Trop Dis; 11(9):e0005929, 2017 Sep.
ISSN:1935-2735
País de publicação:United States
Idioma:eng
Resumo:Four sesquiterpene lactones, mikanolide, deoxymikanolide, dihydromikanolide and scandenolide, were isolated by a bioassay-guided fractionation of Mikania variifolia and Mikania micrantha dichloromethane extracts. Mikanolide and deoxymikanolide were the major compounds in both extracts (2.2% and 0.4% for Mikania variifolia and 21.0% and 6.4% for Mikania micrantha respectively, calculated on extract dry weight). Mikanolide, deoxymikanolide and dihydromikanolide were active against Trypanosoma cruzi epimastigotes (50% inhibitory concentrations of 0.7, 0.08 and 2.5 µg/mL, for each compound respectively). These sesquiterpene lactones were also active against the bloodstream trypomastigotes (50% inhibitory concentrations for each compound were 2.1, 1.5 and 0.3 µg/mL, respectively) and against amastigotes (50% inhibitory concentrations for each compound were 4.5, 6.3 and 8.5 µg/mL, respectively). By contrast, scandenolide was not active on Trypanosoma cruzi. Besides, mikanolide and deoxymikanolide were also active on Leishmania braziliensis promastigotes (50% inhibitory concentrations of 5.1 and 11.5 µg/mL, respectively). The four sesquiterpene lactones were tested for their cytotoxicity on THP 1 cells. Deoxymikanolide presented the highest selectivity index for trypomastigotes (SI = 54) and amastigotes (SI = 12.5). In an in vivo model of Trypanosoma cruzi infection, deoxymikanolide was able to decrease the parasitemia and the weight loss associated to the acute phase of the parasite infection. More importantly, while 100% of control mice died by day 22 after receiving a lethal T. cruzi infection, 70% of deoxymikanolide-treated mice survived. We also observed that this compound increased TNF-α and IL-12 production by macrophages, which could contribute to control T. cruzi infection.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (1,10:2,3-diepoxy-6,8-dihydroxy-11-vinylgermacr-4-ene 12,14-di-gamma-lactone); 0 (Lactones); 0 (Plant Extracts); 0 (Sesquiterpenes); 0 (Sesquiterpenes, Germacrane); 0 (Tumor Necrosis Factor-alpha); 0 (deoxymikanolide); 187348-17-0 (Interleukin-12); 23758-16-9 (scandenolide)


  2 / 87 MEDLINE  
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PMID:28602506
Autor:Wang J; Geng S; Wang B; Shao Q; Fang Y; Wei Y
Endereço:State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 3(rd) Ring North East Road, Chaoyang District, Beijing 100029, China.
Título:Magnetic nanoparticles and high-speed countercurrent chromatography coupled in-line and using the same solvent system for separation of quercetin-3-O-rutinoside, luteoloside and astragalin from a Mikania micrantha extract.
Fonte:J Chromatogr A; 1508:42-52, 2017 Jul 28.
ISSN:1873-3778
País de publicação:Netherlands
Idioma:eng
Resumo:A new in-line method of magnetic nanoparticles (MNPs) coupled with high-speed countercurrent chromatography (HSCCC) using a same solvent system during the whole separation process was established to achieve the rapid separation of flavonoids from Mikania micrantha. The adsorption and desorption capacities of five different MNPs for flavonoid standards and Mikania micrantha crude extract were compared and the most suitable magnetic nanoparticle Fe O @SiO @DIH@EMIMLpro was selected as the in-line MNP column. An in-line separation system was established by combining this MNP column with HSCCC through a six-way valve. The comparison between two solvent systems n-hexane-ethyl acetate-methanol-water (3:5:3:5, v/v) and ethyl acetate-methanol-water (25:1:25, v/v) showed that the latter solvent system was more suitable for simultaneously in-line separating three flavonoids quercetin-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha. The purities of these three compounds with the ethyl acetate-methanol-water solvent system were 95.13%, 98.54% and 98.19% respectively. Results showed the established in-line separation system of MNP-HSCCC was efficient, recyclable and served to isolate potential flavonoids with similar polarities from natural complex mixtures. The in-line combination of magnetic nanoparticles with high-speed countercurrent chromatography eluting with the same solvent system during the whole separation process was established for the first time.
Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE
Nome de substância:0 (Glucosides); 0 (Kaempferols); 0 (Magnetite Nanoparticles); 0 (Plant Extracts); 0 (quercetin-3-O-rutinoside); 98J6XDS46I (luteolin-7-glucoside); 9IKM0I5T1E (Quercetin); APM8UQ3Z9O (astragalin); KUX1ZNC9J2 (Luteolin)


  3 / 87 MEDLINE  
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PMID:28028017
Autor:Geng SL; Chen Q; Cai WL; Cao AC; Ou-Yang CB
Endereço:College of Life Sciences, South China Agricultural University, Guangzhou 510642, China gengshilei@scau.edu.cn.
Título:Genetic variation in the invasive weed Mikania micrantha (Asteraceae) suggests highways as corridors for its dispersal in southern China.
Fonte:Ann Bot; 119(3):457-464, 2017 Feb.
ISSN:1095-8290
País de publicação:England
Idioma:eng
Resumo:BACKGROUND AND AIMS: Roads as corridors of seed or fruit spatial dispersal have major impacts on the establishment and spread of invasive species, but their precise role in population genetic variation remains poorly understood. The South American weed Mikania micrantha has spread rapidly across southern China since its introduction to the Shenzhen area in 1984. This study investigated how its genetic diversity is distributed along highways, and whether highways have acted as corridors for the rapid expansion of M. micrantha METHODS: Twenty-seven roadside populations were sampled along four highways in southern China, and 787 samples were examined using 12 microsatellite markers. Variation in genetic diversity among populations was quantified and patterns of genetic differentiation were analysed. KEY RESULTS: A high level of genetic diversity was found at both the species and the population levels in this self-incompatible plant (expected heterozygosity = 0·497 and 0·477, respectively; allelic richness = 2·580 and 2·521, respectively). The Wright F-statistic value among populations (0·044, P < 0·01) and the analysis of molecular variance (91 % of genetic variation residing within populations, 9 % among populations within highways and 0 % among the four highways) showed a relatively low level of genetic differentiation among populations, while the principal coordinate and cluster analyses also indicated a lack of clear geographical genetic structure among populations. The calculated N value of 5·5 signifies strong gene flow. CONCLUSIONS: The pattern of genetic variation is consistent with facilitated dispersal along highways. The genetic admixtures among the roadside populations imply the occurrence of multiple population introductions during colonization. The long-distance dispersal of seeds associated with vehicular transportation on highways may have played important roles in shaping the genetic variation. This finding highlights the importance of highways as corridors for the spread of M. micrantha in southern China.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Plant)


  4 / 87 MEDLINE  
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ALONSO, ARACI MOLNAR
PMID:27336315
Autor:Agostini-Costa TD; Gomes IS; Fonseca MC; Alonso AM; Pereira RC; Montanari Junior I; da Silva JP; Pereira AM; da Silva DB; Vieira RF; Vaz AP
Endereço:Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
Título:Effect of Accessions and Environment Conditions on Coumarin, O-Coumaric and Kaurenoic Acids Levels of Mikania laevigata.
Fonte:Planta Med; 82(16):1431-1437, 2016 Nov.
ISSN:1439-0221
País de publicação:Germany
Idioma:eng
Resumo:Coumarin, -coumaric, and kaurenoic acid are bioactive compounds usually found in the leaves of . Genetic and environmental variations in the secondary metabolites of plants may have implications for their biological effects. Three different accessions of cultivated in four sites between the Equator and the Tropic of Capricorn in Brazil were evaluated aiming to present potential raw materials and discuss relationships among these three bioactive compounds. The results revealed effects of plant accessions and environmental factors and suggested two contrasting chemical phenotypes of . The first phenotype presented the highest levels of kaurenoic acid (2283 ± 316 mg/100 g) besides lower levels of coumarin (716 ± 61 mg/100 g), which was also stimulated by the environment and mild climate at the site nearest to the Tropic of Capricorn. The other phenotype presented the lowest levels of kaurenoic acid (137 ± 17 mg/100 g) besides higher levels of coumarin (1362 ± 108 mg/100 g), which was also stimulated by the environment and tropical climate at the site nearest to the Equatorial beach.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE
Nome de substância:0 (Coumaric Acids); 0 (Coumarins); 0 (Diterpenes); 6730-83-2 (kaurenoic acid); A4VZ22K1WT (coumarin)


  5 / 87 MEDLINE  
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PMID:27323798
Autor:Shen S; Xu G; Clements DR; Jin G; Liu S; Yang Y; Chen A; Zhang F; Kato-Noguchi H
Endereço:Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650205, Yunnan, China.
Título:Suppression of reproductive characteristics of the invasive plant Mikania micrantha by sweet potato competition.
Fonte:BMC Ecol; 16:30, 2016 Jun 20.
ISSN:1472-6785
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: As a means of biologically controlling Mikania micrantha H.B.K. in Yunnan, China, the influence of sweet potato [Ipomoea batatas (L.) Lam.] on its reproductive characteristics was studied. The trial utilized a de Wit replacement series incorporating six ratios of sweet potato and M. micrantha plants in 25 m(2) plots over 2 years. RESULTS: Budding of M. micrantha occurred at the end of September; flowering and fruiting occurred from October to February. Flowering phenology of M. micrantha was delayed (P < 0.05), duration of flowering and fruiting was reduced (P < 0.05) and duration of bud formation was increased (P < 0.05) with increasing proportions of sweet potato. Reproductive allocation, reproductive investment and reproductive index of M. micrantha were significantly reduced (P < 0.05) with increasing sweet potato densities. Apidae bees, and Calliphoridae or Syrphidae flies were the most abundant visitors to M. micrantha flowers. Overall flower visits decreased (P < 0.05) as sweet potato increased. Thus the mechanism by which sweet potato suppressed sexual reproduction in M. micrantha was essentially two-fold: causing a delay in flowering phenology and reducing pollinator visits. The number, biomass, length, set rate, germination rate, and 1000-grain dry weight of M. micrantha seeds were suppressed (P < 0.05) by sweet potato competition. With proportional increases in sweet potato, sexual and asexual seedling populations of M. micrantha were significantly reduced (P < 0.05). The mortality of both seedling types increased (P < 0.05) with proportional increases in sweet potato. CONCLUSIONS: These results suggest that sweet potato significantly suppresses the reproductive ability of the invasive species M. micrantha, and is a promising alternative to traditional biological control and other methods of control. Planting sweet potato in conjunction with other control methods could provide a comprehensive strategy for managing M. micrantha. The scenario of controlling M. micrantha by utilizing a crop with a similar growth form may provide a useful model for similar management strategies in other systems.
Tipo de publicação: JOURNAL ARTICLE


  6 / 87 MEDLINE  
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PMID:27316976
Autor:Moreira MR; Souza AB; Soares S; Bianchi TC; de Souza Eugênio D; Lemes DC; Martins CH; da Silva Moraes T; Tavares DC; Ferreira NH; Ambrósio SR; Veneziani RC
Endereço:University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Título:ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries.
Fonte:Fitoterapia; 112:211-6, 2016 Jul.
ISSN:1873-6971
País de publicação:Netherlands
Idioma:eng
Resumo:Many studies have reported that medicinal plant extracts can inhibit oral pathogen growth or adhesion to surfaces and therefore reduce dental caries formation. The addition of these extracts to oral products like mouthwashes and dentifrices is considered an important strategy in caries control. In this sense, we have developed a Mikania glomerata extract with high ent-kaurenoic acid content (KAMg). So, this work describes the preparation of such extract and the development of a validated HPLC-DAD method to determine its ent-kaurenoic acid (KA) content. Herein it is also described the KAMg in vitro antibacterial evaluation against several cariogenic bacteria in comparison with KA and the investigation of further aspects of the KAMg activity. Toxicological aspects of the developed extract were evaluated by assessing its cytotoxicity and genotoxicity. KA and a KA-rich extract like KAMg showed to inhibit the growth of microorganisms responsible for dental caries at relatively low MIC (Minimum inhibitory concentration) values, albeit not as low as the MIC value obtained for chlorhexidine digluconate (CHD), the golden anticariogenic standard approved by the American Dental Association Council on Dental Therapeutics. However, KAMg was more effective to inhibit the formation of a Streptococcus mutans biofilm with four times lower MICB50 (minimum inhibitory concentration that reduces 50% of the biofilm) value as compared with CHD. Taking into account all these data and considering the absence of genotoxic and cytotoxic activity under the tested conditions, it is suggested that KAMg is a natural product to be considered as active ingredient in oral care products.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (Diterpenes); 0 (Plant Extracts); 6730-83-2 (kaurenoic acid)


  7 / 87 MEDLINE  
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PMID:26815365
Autor:Huang F; Peng S
Endereço:State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Título:Intraspecific competitive ability declines towards the edge of the expanding range of the invasive vine Mikania micrantha.
Fonte:Oecologia; 181(1):115-23, 2016 May.
ISSN:1432-1939
País de publicação:Germany
Idioma:eng
Resumo:The evolution of competitive ability plays an important role in plant invasions. While many studies of the evolution of invasive species have compared populations from native and invaded ranges in terms of their performance, little attention has been paid to the evolution of intraspecific competitive ability within the invaded range during range expansion. In addition, whether the proportional change in the amount of invasive litter influences the intraspecific competitive ability among invasive populations of different ages has not yet been investigated. Here we selected Mikania micrantha H.B.K., a highly invasive vine in south China with a well-documented invasion history, as the study species. We manipulated competition among populations of different ages from the core of the range to its edges under four litter treatments in a common garden experiment. We found that during its 30-year invasion, intraspecific competitive ability was rapidly selected against towards range edges, which may be driven partly by the decline in population density. However, litter source did not influence the outcome of the competition among populations of different ages; it instead functioned more like a supply of nutrients. We suggest that stage-specific conditions such as population density should be incorporated into the experimental design when examining the evolution of invasive plants, especially when invasive populations are subject to selection on a small geographic scale. This approach can reduce sampling bias and thus improve the ability to infer the mechanisms responsible for the evolution of invasive populations.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T


  8 / 87 MEDLINE  
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PMID:26214408
Autor:Polonio JC; Almeida TT; Garcia A; Mariucci GE; Azevedo JL; Rhoden SA; Pamphile JA
Endereço:Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Título:Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens.
Fonte:Genet Mol Res; 14(3):7297-309, 2015 Jul 03.
ISSN:1676-5680
País de publicação:Brazil
Idioma:eng
Resumo:Mikania glomerata (Spreng.), popularly known as "guaco", is a plant from the Asteraceae family that has many therapeutic properties. The use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. Microorganism-host complex interactions are related to the production of compounds that may confer resistance to pathogens or to production of bioactive compounds or growth regulators. In this study, we evaluated foliar endophytic fungi of M. glomerata to examine the control of plant pathogens, molecular identification, and production of compounds with antimicrobial activity. In the antagonism test, 6-mm diameter disks were placed equidistant from the endophyte and plant pathogen, and pathogen growth area was measured. The endophytic strains G-01, G-02, and G-03 were effective against Fusarium solani and Didymella bryoniae. The endophyte rDNA regions corresponding to internal transcribed spacer 1-5.8S-internal transcribed spacer 2 were sequenced, and the results were compared with sequences deposited in the NCBI database. The G-01, G-02, and G-03 strains were identified as Diaporthe citri. This identification was confirmed by phylogenetic analysis. The crude extract of the secondary metabolites of the G-01 strain was tested against Escherichia coli and Staphylococcus aureus; the metabolites showed antimicrobial activity against S. aureus. The endophytes tested in this study have potential for use in biotechnological applications.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (Antifungal Agents)


  9 / 87 MEDLINE  
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PMID:26177031
Autor:Wu AP; Li ZL; He FF; Wang YH; Dong M
Endereço:Ecology Department, College of Bioscience & Biotechnology, Institute of Ecology, Hunan Agricultural University, Changsha, 410128, China.
Título:Screening Allelochemical-Resistant Species of the Alien Invasive Mikania micrantha for Restoration in South China.
Fonte:PLoS One; 10(7):e0132967, 2015.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:To screen allelochemical-resistant species of the alien invasive weed Mikania micrantha, we studied the allelopathic inhibition effects of the leaf aqueous extract (LAE) of Mikania on seed germination and seedling growth of the 26 species native or naturalized in the invaded region in South China. Seed germination was more strongly negatively affected by LAE than seedling growth. Responses of seed germination and seed growth to LAE differed differently among the target species. LAE more strongly negatively affected seed germination, but less strongly negatively affected seedling growth, in non-legume species than in legume species. LAE more strongly negatively affected seed germination and seedling growth in native species than naturalized exotic species. Therefore, naturalized exotic non-legume seedlings are more suitable than seeds of native legume species for restoration of Mikania-invaded habitats.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Plant Extracts)


  10 / 87 MEDLINE  
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PMID:25757073
Autor:Gasparetto JC; Peccinini RG; de Francisco TM; Cerqueira LB; Campos FR; Pontarolo R
Endereço:Department of Pharmacy, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Título:A kinetic study of the main guaco metabolites using syrup formulation and the identification of an alternative route of coumarin metabolism in humans.
Fonte:PLoS One; 10(3):e0118922, 2015.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:For decades guaco species have been empirically used for the treatment of respiratory diseases. However, studies have shown that the toxic and therapeutic effects of the main guaco metabolites are dose-dependent, and none clinical study was done to evaluate the behavior of these substances in humans. In this work, a pilot study measuring the kinetic profile of the main guaco metabolites was performed leading to the knowledge of an alternative route of coumarin metabolism in humans. Initial screenings demonstrated that the administration of 60 mL of guaco syrup (single dose) did not provide sufficient levels of coumarin (COU), 7-hydroxycoumarin (7-HCOU), o-coumaric acid (OCA) and kaurenoic acid (KAU). The pharmacokinetic parameters were calculated by orally administering 60 mL of guaco syrup spiked with 1500 mg of COU. The kinetic study demonstrated that the plasmatic levels of 7-HCOU (considered the main metabolite of COU) were 10 times lower than the levels of COU, and the kinetic profile of 7-HCOU suggests sequential metabolism in the liver with low access of 7-HCOU to the systemic circulation. The study also demonstrated that OCA is one of the main bioavailable metabolites of COU. Therefore, the hydrolysis of the lactone ring forming a carboxylated compound is one of the possible routes of COU metabolism in humans. The half-lives of COU, 7-HCOU and OCA were approximately 4.0, 1.0 and 3.0 h, respectively and there was evidence that the recommended dosage of guaco syrup did not provide sufficient levels of COU, 7-HCOU or OCA to obtain a bronchodilation effect. Clinical studies are necessary to prove the efficacy and safety of products based on guaco.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Coumarins); 0 (Drug Combinations); 0 (Plant Extracts); 0 (Respiratory System Agents); 0 (Umbelliferones); 60Z60NTL4G (7-hydroxycoumarin); A4VZ22K1WT (coumarin)



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