Base de dados : MEDLINE
Pesquisa : B03.510.460.400.400 [Categoria DeCS]
Referências encontradas : 67 [refinar]
Mostrando: 1 .. 10   no formato [Longo]

página 1 de 7 ir para página                  

  1 / 67 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
PMID:23545654
Autor:Yokoyama H; Yamashita T; Horikoshi N; Kurumizaka H; Kagawa W
Endereço:Animal Waste Management and Environment Division, National Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan. hiroshiy@affrc.go.jp
Título:Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii.
Fonte:Acta Crystallogr Sect F Struct Biol Cryst Commun; 69(Pt 4):438-40, 2013 Apr 01.
ISSN:1744-3091
País de publicação:England
Idioma:eng
Resumo:The Athe_0614 protein is a component of the extracellular proteins secreted by the anaerobic, extremely thermophilic and cellulolytic bacterium Caldicellulosiruptor bescii. The recombinant protein was expressed in Escherichia coli, purified to near-homogeneity and crystallized using polyethylene glycol 2000 monomethyl ether as a precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 48.4, b = 42.2, c = 97.8 Å, ß = 96.1°, and diffracted to 2.7 Å resolution using synchrotron radiation.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Bacterial Proteins)


  2 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:21216591
Autor:Lim YR; Yeom SJ; Kim YS; Oh DK
Endereço:Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, South Korea.
Título:Synergistic production of L-arabinose from arabinan by the combined use of thermostable endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus.
Fonte:Bioresour Technol; 102(5):4277-80, 2011 Mar.
ISSN:1873-2976
País de publicação:England
Idioma:eng
Resumo:The optimum conditions for the production of L-arabinose from debranched arabinan were determined to be pH 6.5, 75°C, 20 g l(-1) debranched arabinan, 42 Um l(-1) endo-1,5-α-L-arabinanase, and 14 U ml(-1) α-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus and the conditions for sugar beet arabinan were pH 6.0, 75°C, 20 g l(-1) sugar beet arabinan, 3 U ml(-1) endo-1,5-α-L-arabinanase, and 24 U ml(-1) α-L-arabinofuranosidase. Under the optimum conditions, 16 g l(-1)l-arabinose was obtained from 20 g l(-1) debranched arabinan or sugar beet arabinan after 120 min, with a hydrolysis yield of 80% and a productivity of 8 g l(-1)h(-1). This is the first reported trial for the production of L-arabinose from the hemicellulose arabinan by the combined use of endo- and exo-arabinanases.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Polysaccharides); 11078-27-6 (araban); B40ROO395Z (Arabinose); EC 3.2.1.- (Glycoside Hydrolases)


  3 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:19196756
Autor:Slepova TV; Sokolova TG; Kolganova TV; Tourova TP; Bonch-Osmolovskaya EA
Endereço:Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60 Let Oktyabrya 7/2, 117312 Moscow, Russia. slepysh@gmail.com
Título:Carboxydothermus siderophilus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring.
Fonte:Int J Syst Evol Microbiol; 59(Pt 2):213-7, 2009 Feb.
ISSN:1466-5026
País de publicação:England
Idioma:eng
Resumo:A novel anaerobic, thermophilic, Fe(III)-reducing, CO-utilizing bacterium, strain 1315(T), was isolated from a hot spring of Geyser Valley on the Kamchatka Peninsula. Cells of the new isolate were Gram-positive, short rods. Growth was observed at 52-70 degrees C, with an optimum at 65 degrees C, and at pH 5.5-8.5, with an optimum at pH 6.5-7.2. In the presence of Fe(III) or 9,10-anthraquinone 2,6-disulfonate (AQDS), the bacterium was capable of growth with CO and yeast extract (0.2 g l(-1)); during growth under these conditions, strain 1315(T) produced H(2) and CO(2) and Fe(II) or AQDSH(2), respectively. Strain 1315(T) also grew by oxidation of yeast extract, glucose, xylose or lactate under a N(2) atmosphere, reducing Fe(III) or AQDS. Yeast extract (0.2 g l(-1)) was required for growth. Isolate 1315(T) grew exclusively with Fe(III) or AQDS as an electron acceptor. The generation time under optimal conditions with CO as growth substrate was 9.3 h. The G+C content of the DNA was 41.5+/-0.5 mol%. 16S rRNA gene sequence analysis placed the organism in the genus Carboxydothermus (97.8 % similarity with the closest relative). On the basis of physiological features and phylogenetic analysis, it is proposed that strain 1315(T) should be assigned to a novel species, Carboxydothermus siderophilus sp. nov., with the type strain 1315(T) (=VKPM 9905B(T) =VKM B-2474(T) =DSM 21278(T)).
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Ferric Compounds); 0 (RNA, Ribosomal, 16S); 142M471B3J (Carbon Dioxide); 1K09F3G675 (ferric oxide); 7YNJ3PO35Z (Hydrogen)


  4 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:19060086
Autor:Miroshnichenko ML; Tourova TP; Kolganova TV; Kostrikina NA; Chernych N; Bonch-Osmolovskaya EA
Endereço:Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
Título:Ammonifex thiophilus sp. nov., a hyperthermophilic anaerobic bacterium from a Kamchatka hot spring.
Fonte:Int J Syst Evol Microbiol; 58(Pt 12):2935-8, 2008 Dec.
ISSN:1466-5026
País de publicação:England
Idioma:eng
Resumo:A novel anaerobic, extremely thermophilic, facultatively chemolithoautotrophic bacterium designated strain SR(T) was isolated from a terrestrial hot spring in Kamchatka (Russia). The cells of the novel strain were spore-forming rods with a Gram-positive type of cell wall. The novel isolate grew at 60-82 degrees C (optimum 75 degrees C) and pH 6.0-7.5 (optimum 6.8). Molecular hydrogen and formate were used as electron donors. Thiosulfate, sulfate or elemental sulfur served as electron acceptors yielding hydrogen sulfide. No growth was observed on either substrate in the presence of nitrate as the electron acceptor. The G+C content of the DNA was 56.2 mol%. Phylogenetic analyses of the 16S rRNA gene showed that strain SR(T) was most closely related to Ammonifex degensii (96.4 % gene sequence similarity). However, the novel isolate possessed phenotypic characteristics that differed significantly from those of A. degensii, the only other recognized species of the genus Ammonifex. It is concluded that strain SR(T) (=DSM 19636(T)=VKM B-2461(T)) represents the type strain of a novel species of the genus Ammonifex, for which the name Ammonifex thiophilus sp. nov. is proposed. An emendation of the genus Ammonifex is proposed based on the phenotypic characteristics of the novel species.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (RNA, Ribosomal, 16S)


  5 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
PMID:18293927
Autor:Doukov TI; Blasiak LC; Seravalli J; Ragsdale SW; Drennan CL
Endereço:Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Título:Xenon in and at the end of the tunnel of bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase.
Fonte:Biochemistry; 47(11):3474-83, 2008 Mar 18.
ISSN:0006-2960
País de publicação:United States
Idioma:eng
Resumo:A fascinating feature of some bifunctional enzymes is the presence of an internal channel or tunnel to connect the multiple active sites. A channel can allow for a reaction intermediate generated at one active site to be used as a substrate at a second active site, without the need for the intermediate to leave the safety of the protein matrix. One such bifunctional enzyme is carbon monoxide dehydrogenase/acetyl-CoA synthase from Moorella thermoacetica (mtCODH/ACS). A key player in the global carbon cycle, CODH/ACS uses a Ni-Fe-S center called the C-cluster to reduce carbon dioxide to carbon monoxide and uses a second Ni-Fe-S center, called the A-cluster, to assemble acetyl-CoA from a methyl group, coenzyme A, and C-cluster-generated CO. mtCODH/ACS has been proposed to contain one of the longest enzyme channels (138 A long) to allow for intermolecular CO transport. Here, we report a 2.5 A resolution structure of xenon-pressurized mtCODH/ACS and examine the nature of gaseous cavities within this enzyme. We find that the cavity calculation program CAVENV accurately predicts the channels connecting the C- and A-clusters, with 17 of 19 xenon binding sites within the predicted regions. Using this X-ray data, we analyze the amino acid composition surrounding the 19 Xe sites and consider how the protein fold is utilized to carve out such an impressive interior passageway. Finally, structural comparisons of Xe-pressurized mtCODH/ACS with related enzyme structures allow us to study channel design principles, as well as consider the conformational flexibility of an enzyme that contains a cavity through its center.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
Nome de substância:0 (Multienzyme Complexes); 3H3U766W84 (Xenon); EC 1.2.- (Aldehyde Oxidoreductases); EC 1.2.99.2 (carbon monoxide dehydrogenase); EC 6.2.1.1 (Acetate-CoA Ligase)


  6 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PMID:16449442
Autor:Slobodkin AI; Tourova TP; Kostrikina NA; Lysenko AM; German KE; Bonch-Osmolovskaya EA; Birkeland NK
Endereço:Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117 312 Moscow, Russia. aslobodkin@hotmail.com
Título:Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales.
Fonte:Int J Syst Evol Microbiol; 56(Pt 2):369-72, 2006 Feb.
ISSN:1466-5026
País de publicação:England
Idioma:eng
Resumo:A moderately thermophilic, anaerobic bacterium (strain SB91T) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91T were straight to slightly curved rods, 0.5-0.6 microm in diameter and 3.0-7.0 mum in length. Formation of endospores was not observed. The temperature range for growth was 26-62 degrees C, with an optimum at 50 degrees C. The pH range for growth was 5.5-9.5, with an optimum at pH 7.5-8.0. The substrates utilized by strain SB91T in the presence of 9,10-anthraquinone 2,6-disulfonate included peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, alanine plus glycine, alanine plus proline, L-valine and n-propanol. Carbohydrates were not utilized. Strain SB91T reduced amorphous Fe(III) oxide, Fe(III) citrate, Fe(III) EDTA or Fe(III) nitrilotriacetate with peptone, L-valine or n-propanol as an electron donor. Strain SB91T reduced 9,10-anthraquinone 2,6-disulfonate, thiosulfate, elemental sulfur, fumarate and selenite. Strain SB91T survived after exposure to gamma-radiation at a dose of 5.4 kGy. The G+C content of the DNA of strain SB91T was 33 mol%. Analysis of the 16S rRNA gene sequence revealed that the isolated organism belonged to cluster XII of the clostridia. On the basis of its physiological properties and the results of phylogenetic analyses, it is proposed that strain SB91T represents the sole species of a novel genus, Tepidimicrobium; the name Tepidimicrobium ferriphilum gen. nov., sp. nov. is proposed, with strain SB91T (=DSM 16624T=VKM B-2348T) as the type strain.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Ferric Compounds); 0 (RNA, Ribosomal, 16S); E1UOL152H7 (Iron)


  7 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PMID:16316733
Autor:Morais PV; Paulo C; Francisco R; Branco R; Paula Chung A; da Costa MS
Endereço:Departamento Bioquímica, Faculdade de Ciências e Tecnologia da Universidade de Coimbra, Apartado 3126, 3001-401 Coimbra, Portugal. pvmorais@ci.uc.pt
Título:Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress.
Fonte:Syst Appl Microbiol; 29(5):414-21, 2006 Jul.
ISSN:0723-2020
País de publicação:Germany
Idioma:eng
Resumo:Two strains designated RF6(T) and RB10(T) were isolated, from activated sludge and from river sediments, respectively, both systems receiving chromium contaminated water. Phylogenetic analysis showed that strain RF6(T)and strain RB10(T) represented two new species of the genus Leucobacter. Strain RB10(T) can be distinguished from RF6(T) by its ability to grow at 37 degrees C, by showing a different optimum pH, by cell wall amino acids different relative amount and by having the fatty acid strait C16:0 as the third most abundant fatty acid. On the basis of the distinct peptidoglycan composition, 16S ribosomal DNA sequence analysis, DNA-DNA reassociation values, and phenotypic characteristics we are of the opinion that strain RF6(T) represents a new species of the genus Leucobacter for which we propose the name Leucobacter luti (CIP 108818(T)=LMG 23118) and that strain RB10(T) represents an additional new species of the same genus for which we propose the name Leucobacter alluvii (CIP 108819(T)=LMG 23117).
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Sewage); 0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium)


  8 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PMID:15850383
Autor:Silaghi-Dumitrescu R; Kurtz DM; Ljungdahl LG; Lanzilotta WN
Endereço:Department of Chemistry, and Center for Metalloenzyme Studies, University of Georgia, Athens, Georgia 30602, USA.
Título:X-ray crystal structures of Moorella thermoacetica FprA. Novel diiron site structure and mechanistic insights into a scavenging nitric oxide reductase.
Fonte:Biochemistry; 44(17):6492-501, 2005 May 03.
ISSN:0006-2960
País de publicação:United States
Idioma:eng
Resumo:Several members of a widespread class of bacterial and archaeal metalloflavoproteins, called FprA, likely function as scavenging nitric oxide reductases (S-NORs). However, the only published X-ray crystal structure of an FprA is for a protein characterized as a rubredoxin:dioxygen oxidoreductase (ROO) from Desulfovibrio gigas. Therefore, the crystal structure of Moorella thermoacetica FprA, which has been established to function as an S-NOR, was solved in three different states: as isolated, reduced, and reduced, NO-reacted. As is the case for D. gigas ROO, the M. thermoacetica FprA contains a solvent-bridged non-heme, non-sulfur diiron site with five-coordinate iron centers bridged by an aspartate, and terminal glutamate, aspartate, and histidine ligands. However, the M. thermoacetica FprA diiron site showed four His ligands, two to each iron, in all three states, whereas the D. gigas ROO diiron site was reported to contain only three His ligands, even though the fourth His residue is conserved. The Fe1-Fe2 distance within the diiron site of M. thermoacetica FprA remained at 3.2-3.4 A with little or no movement of the protein ligands in the three different states and with conservation of the two proximal open coordination sites. Molecular modeling indicated that each open coordination site can accommodate an end-on NO. This relatively rigid and symmetrical diiron site structure is consistent with formation of a diferrous dinitrosyl as the committed catalytic intermediate leading to formation of N(2)O. These results provide new insight into the structural features that fine-tune biological non-heme diiron sites for dioxygen activation vs nitric oxide reduction.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
Nome de substância:0 (Flavoproteins); 0 (Free Radical Scavengers); 42HK56048U (Tyrosine); 4QD397987E (Histidine); 7N464URE7E (Flavin Mononucleotide); E1UOL152H7 (Iron); EC 1.- (Oxidoreductases); EC 1.18.1.1 (rubredoxin-NAD+ reductase); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.7.2.5 (nitric-oxide reductase)


  9 / 67 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PMID:12086178
Autor:Ringø E; Seppola M; Berg A; Olsen RE; Schillinger U; Holzapfel W
Endereço:Department of Arctic Veterinary Medicine, The Norwegian School of Veterinary Science, Tromsø. Einar.Ringo@veths.no
Título:Characterization of Carnobacterium divergens strain 6251 isolated from intestine of Arctic charr (Salvelinus alpinus L.).
Fonte:Syst Appl Microbiol; 25(1):120-9, 2002 Apr.
ISSN:0723-2020
País de publicação:Germany
Idioma:eng
Resumo:An atypical strain of Carnobacterium divergens, strain 6251, was isolated from the small intestine of Arctic charr (Salvelinus alpinus L.), fed high dietary carbohydrate. This strain showed marked growth inhibitory effects in vitro against the fish pathogens Aeromonas salmonicida subsp. salmonicida (furunculosis), Vibrio anguillarum (vibriosis) and Vibrio viscocus (winter ulcer). The strain is a non-motile Gram-positive psychrotrophic rod that lacks both catalase and oxidase, grows at pH 9.1 (CTAS agar), but not on acetate containing media (pH < or = 5.4), on TCBS or at < or =6% sodium chloride content. Strain 6251 is facultatively anaerobic and utilises tryptone as a sole source of nutrient. Further characterisation showed the most abundant cellular fatty acid of strain 6251 to be oleic acid (18:1) (n-9) (36.0%). Sequencing of a 16S rDNA region of 578 nucleotides and AFLP microbial fingerprinting suggested that strain 6251 is not closely related to any carnobacteria known, however, DNA-DNA similarity determinations showed high similarity (96.2%) with the type strain of Carnobacterium divergens. The unique phenotypic attributes of this strain represent new information on the biodiversity and ecology of carnobacteria and especially of the species C. divergens.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (RNA, Ribosomal, 16S)


  10 / 67 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PMID:12029528
Autor:Jones CR; Ray M; Strobel HJ
Endereço:Department of Animal Sciences, 212 W.P. Garrigus Building, University of Kentucky, Lexington, KY 40546-0215, USA.
Título:Transcriptional analysis of the xylose ABC transport operons in the thermophilic anaerobe Thermoanaerobacter ethanolicus.
Fonte:Curr Microbiol; 45(1):54-62, 2002 Jul.
ISSN:0343-8651
País de publicação:United States
Idioma:eng
Resumo:Thermoanaerobacter ethanolicus is a Gram-positive thermophile that converts xylose to ethanol. A portion of the T. ethanolicus xylose transport permease gene ( xylH) was cloned, and the deduced protein exhibited greater than 60% similarity to homologs in enterobacteria. Xylose-binding protein ( xylF) and ( xylH) transcripts were quantitated and compared from cells grown in batch or continuous cultures grown on xylose, glucose, or a mixture of both sugars. In contrast to the strong repression of xyl operons by glucose in other bacteria, both xylF and xylH expression were detected in the presence of this hexose sugar. Expression of xylF and xylH generally increased with dilution rate (3- and 1.5-fold, respectively) and seemed to be growth rate rather than substrate dependent. Overall, these unusual sugar utilization patterns in batch and continuous culture seem to result from a basal expression level of xyl genes in the absence of xylose. T. ethanolicus is unique in possessing a triumvirate of xylose transport and catabolism operons and, given its extensive hemicellulolytic capabilities, may have evolved to constitutively express xyl genes.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE
Nome de substância:0 (Carbohydrates); 0 (Carrier Proteins); 0 (Escherichia coli Proteins); 0 (Symporters); 0 (xylE protein, E coli); A1TA934AKO (Xylose)



página 1 de 7 ir para página                  
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde