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Pesquisa : B03.510.460.400.400.049.049 [Categoria DeCS]
Referências encontradas : 626 [refinar]
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  1 / 626 MEDLINE  
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PMID:29216246
Autor:Yassin AF; Langenberg S; Huntemann M; Clum A; Pillay M; Palaniappan K; Varghese N; Mikhailova N; Mukherjee S; Reddy TBK; Daum C; Shapiro N; Ivanova N; Woyke T; Kyrpides NC
Endereço:Institut für medizinische Mikrobiologie und Immunologie der Universität Bonn, Bonn, Germany.
Título:Draft genome sequence of Actinotignum schaalii DSM 15541T: Genetic insights into the lifestyle, cell fitness and virulence.
Fonte:PLoS One; 12(12):e0188914, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:The permanent draft genome sequence of Actinotignum schaalii DSM 15541T is presented. The annotated genome includes 2,130,987 bp, with 1777 protein-coding and 58 rRNA-coding genes. Genome sequence analysis revealed absence of genes encoding for: components of the PTS systems, enzymes of the TCA cycle, glyoxylate shunt and gluconeogensis. Genomic data revealed that A. schaalii is able to oxidize carbohydrates via glycolysis, the nonoxidative pentose phosphate and the Entner-Doudoroff pathways. Besides, the genome harbors genes encoding for enzymes involved in the conversion of pyruvate to lactate, acetate and ethanol, which are found to be the end products of carbohydrate fermentation. The genome contained the gene encoding Type I fatty acid synthase required for de novo FAS biosynthesis. The plsY and plsX genes encoding the acyltransferases necessary for phosphatidic acid biosynthesis were absent from the genome. The genome harbors genes encoding enzymes responsible for isoprene biosynthesis via the mevalonate (MVA) pathway. Genes encoding enzymes that confer resistance to reactive oxygen species (ROS) were identified. In addition, A. schaalii harbors genes that protect the genome against viral infections. These include restriction-modification (RM) systems, type II toxin-antitoxin (TA), CRISPR-Cas and abortive infection system. A. schaalii genome also encodes several virulence factors that contribute to adhesion and internalization of this pathogen such as the tad genes encoding proteins required for pili assembly, the nanI gene encoding exo-alpha-sialidase, genes encoding heat shock proteins and genes encoding type VII secretion system. These features are consistent with anaerobic and pathogenic lifestyles. Finally, resistance to ciprofloxacin occurs by mutation in chromosomal genes that encode the subunits of DNA-gyrase (GyrA) and topisomerase IV (ParC) enzymes, while resistant to metronidazole was due to the frxA gene, which encodes NADPH-flavin oxidoreductase.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 8558G7RUTR (Pyruvic Acid)


  2 / 626 MEDLINE  
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PMID:28257873
Autor:Karstrup CC; Pedersen HG; Jensen TK; Agerholm JS
Endereço:Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 68, DK-1870 Frederiksberg, Denmark.
Título:Bacterial invasion of the uterus and oviducts in bovine pyometra.
Fonte:Theriogenology; 93:93-98, 2017 Apr 15.
ISSN:1879-3231
País de publicação:United States
Idioma:eng
Resumo:Pyometra is a common disease of cattle that causes infertility and thereby financial losses to the cattle industry. Bacteria involved in the development and progression of pyometra have been investigated by microbial culture but their tissue invading abilities, which is an important aspect of bacterial pathogenicity and development of lesions, have not been investigated. Bacterial invasion of the uterus and oviducts was studied in 21 cows diagnosed with pyometra at the time of slaughter by applying fluorescence in situ hybridization using probes targeting 16S ribosomal RNA of Fusobacterium necrophorum, Porphyromonas levii, Trueperella pyogenes and the overall bacterial domain Bacteria. Fusobacterium necrophorum and P. levii were found to invade the endometrium, especially if the endometrium was ulcerated, and penetrated deep into the lamina propria. These species co-localized within the tissue thus indicating a synergism. Trueperella pyogenes did not invade the uterine tissue. In addition to endometrial lesions, most cows with pyometra also had salpingitis but without significant bacterial invasion of the oviductal wall.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (RNA, Ribosomal, 16S)


  3 / 626 MEDLINE  
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PMID:28246411
Autor:Paetsch C; Fenton K; Perrett T; Janzen E; Clark T; Shearer J; Jelinski M
Endereço:Paetsch Veterinary Services, Muenster, Saskatchewan (Paetsch); Feedlot Health Management Services, Oktotoks, Alberta (Fenton, Perrett); Production Animal Health, University of Calgary Faculty of Veterinary Medicine, Calgary, Alberta (Janzen, Clark); Veterinary Diagnostic and Production Animal Medici
Título:Prospective case-control study of toe tip necrosis syndrome (TTNS) in western Canadian feedlot cattle.
Fonte:Can Vet J; 58(3):247-254, 2017 Mar.
ISSN:0008-5286
País de publicação:Canada
Idioma:eng
Resumo:A case-control study was conducted to investigate potential risk factors for toe tip necrosis syndrome (TTNS) in western Canadian feedlot cattle. Feedlot veterinarians provided hooves from 222 animals that died of either TTNS ("cases") or from all other causes ("controls"). The claws were sectioned by researchers to confirm the diagnoses; there was very good agreement between the practitioners' field diagnosis and that of the researchers (Cohen's kappa = 0.81; < 0.001). The sole thickness of the apical white line region was thinner ( < 0.001) in the cases (3.74 mm) than the controls (4.72 mm). Claws from cases were 5.0 [95% confidence interval (CI): 1.5 to 8.6; < 0.001] and 7.3 times (95% CI: 1.5 to 69.3; < 0.01) more likely than those of controls to yield a heavy growth of and respectively. Cases were 4.4 times (95% CI: 4.4 to 22.9; < 0.001) more likely to be acutely/transiently infected with bovine viral diarrhea virus than were controls. The findings support the hypothesis that TTNS is initiated by excessive wear along the white line, leading to separation and bacterial colonization of the 3rd phalangeal bone (P3) and associated soft tissues.
Tipo de publicação: JOURNAL ARTICLE


  4 / 626 MEDLINE  
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PMID:28188901
Autor:Zhang D; Zhao J; Wang Q; Liu Y; Tian C; Zhao Y; Yu L; Liu M
Endereço:Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
Título:Trueperella pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China: Tetracycline susceptibility and tetracycline-resistance gene distribution.
Fonte:Microb Pathog; 105:51-56, 2017 Apr.
ISSN:1096-1208
País de publicação:England
Idioma:eng
Resumo:Trueperella pyogenes plays a crucial role in endometritis pathogenesis and is also associated with many infections, including metritis, mastitis, arthritis and liver abscessation, in many domestic animals. In this study, we investigated the prevalence of tetracycline resistance in T. pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China, and we assessed tetracycline-resistance gene distribution among the isolates. Our results indicated that 68.7% and 62.5% of the isolates were resistant to tetracycline and doxycycline, respectively, and the rate of resistance to metacycline was 18.8%. The tetracycline resistance gene tetK was present in all isolates (n = 32), whereas the tetM gene was identified in 12.5% and 9.4% of the isolates, in the chromosome and plasmid, respectively. Strains carrying tetW were also common in the chromosome and plasmid, with abundances of 53.1% and 46.9%, respectively. However, tetO and otrA were absent in all isolates. The resistance phenotype analysis indicated that 6.3% of strains were susceptible to all tetracyclines, while 3.1% showed resistance to all tetracyclines.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (Tetracyclines)


  5 / 626 MEDLINE  
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PMID:28144921
Autor:Zhao K; Li W; Huang T; Song X; Zhang X; Yue B
Endereço:Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.
Título:Comparative transcriptome analysis of Trueperella pyogenes reveals a novel antimicrobial strategy.
Fonte:Arch Microbiol; 199(5):649-655, 2017 Jul.
ISSN:1432-072X
País de publicação:Germany
Idioma:eng
Resumo:Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T. pyogenes. Protein crystal structure prediction and primary functional assessment suggested that, the quorum-sensing signal molecules of Gram-negative bacteria such as Pseudomonas aeruginosa and Escherichia coli could significantly inhibit the growth, biofilm production and hemolysis of T. pyogenes by binding to the upstream sensor histidine kinase, PloS. Therefore, the PloS/PlosR two-component regulatory system might dominate the virulence of T. pyogenes. Our findings provide a major advance in understanding the pathogenesis of T. pyogenes, and may shed new light on the development of novel therapeutic strategies to control T. pyogenes infection.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Infective Agents); 0 (DNA-Binding Proteins); 0 (Virulence Factors); EC 2.7.13.1 (Histidine Kinase)


  6 / 626 MEDLINE  
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PMID:27957598
Autor:Pedersen H; Senneby E; Rasmussen M
Endereço:Department of Clinical Sciences, Division of Infection Medicine, Lund University, BMC, B14, Tornavägen 10, 22184, Lund, Sweden.
Título:Clinical and microbiological features of Actinotignum bacteremia: a retrospective observational study of 57 cases.
Fonte:Eur J Clin Microbiol Infect Dis; 36(5):791-796, 2017 May.
ISSN:1435-4373
País de publicação:Germany
Idioma:eng
Resumo:The purpose of this study was to investigate the incidence, clinical presentation, and prognosis of Actinotignum bacteremia in southern Sweden. Actinotignum isolates in blood cultures were identified retrospectively between 1st January 2012 and 31st March 2016 through searches in the clinical microbiology laboratory database. The population covered by this laboratory is approximately 1.3 million. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for species determination. Etests were used for minimum inhibitory concentration (MIC) determination. The patients' medical charts were reviewed. Fifty-eight episodes in fifty-seven patients with Actinotignum bacteremia were identified (A. schaalii = 53, A. sanguinis = 1, A. urinale = 2, and Actinotignum species = 3), which corresponds to an incidence of 11 cases per million inhabitants. Fifty-one percent of the isolates were in pure culture. The MICs were low for ß-lactam antibiotics, whereas high MICs were recorded for ciprofloxacin and trimethoprim. Patients had a median age of 82 years, 72% were male, and a majority had underlying urological conditions. Thirty-six of the patients were diagnosed with a focus from the urinary tract. Thirty-one patients developed severe sepsis and nine patients died during the hospital stay. Our study is the largest of Actinotignum bacteremia and demonstrates that it is a condition with a significant fatality that affects elderly persons with underlying conditions. ß-Lactams represent a rational treatment option.
Tipo de publicação: JOURNAL ARTICLE; OBSERVATIONAL STUDY
Nome de substância:0 (Anti-Bacterial Agents)


  7 / 626 MEDLINE  
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PMID:27940245
Autor:Vallet A; Noël N; Bahi R; Teicher E; Quertainmont Y; Delfraissy JF; Ferlicot S; Potron A; Goujard C; Lambotte O
Endereço:Assistance Publique Hôpitaux de Paris, Service de Médecine Interne et Immunologie Clinique, Hôpitaux Universitaires Paris Sud, Le Kremlin Bicêtre, France.
Título:Recurrent obstructive acute pyelonephritis: A rare form of Actinotignum (Actinobaculum) schaalii infection in a HIV-1 infected patient.
Fonte:Anaerobe; 43:75-77, 2017 Feb.
ISSN:1095-8274
País de publicação:England
Idioma:eng
Resumo:Actinobaculum schaalii is a rarely reported, anaerobic, Gram-positive bacterium which role as uropathogen is emerging. We report here the case of a 47 year old HIV-1 infected woman presented with five recurrent episodes of obstructive pyelonephritis in the context of multiple renal stones. No bacteria was found until the fifth episode, during which prolonged urinary cultures as well as 16S rDNA sequencing allowed the diagnosis of A. schaalii infection. She had developed a life-threatening condition with severe renal failure. A right nephrectomy was performed and found that the intrarenal stones were attributed to the antiretroviral therapy. The renal parenchyma corresponded to an end-stage renal disease with chronic pyelonephritis without abcesses or granules. The situation improved after six months of amoxicillin therapy.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 804826J2HU (Amoxicillin)


  8 / 626 MEDLINE  
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PMID:27913316
Autor:Maraki S; Evangelou G; Stafylaki D; Scoulica E
Endereço:Department of Clinical Microbiology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Crete, Greece. Electronic address: sofiamaraki@in.gr.
Título:Actinotignum schaalii subcutaneous abscesses in a patient with hidradenitis suppurativa: Case report and literature review.
Fonte:Anaerobe; 43:43-46, 2017 Feb.
ISSN:1095-8274
País de publicação:England
Idioma:eng
Resumo:Actinotignum schaalii (formerly Actinobaculum schaalii) is a Gram-positive, facultative anaerobic rod that is typically involved in urinary tract infections in elderly patients or those with underlying urological pathologies. In contrast, abscess formation caused by A. schaalii is very rare. We present a case of multiple abscesses in the perineal area in a young patient with hidradenitis suppurativa associated with A. schaalii and Prevotella melaninogenica and review the relevant literature on the topic.
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE; REVIEW
Nome de substância:0 (DNA, Ribosomal)


  9 / 626 MEDLINE  
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PMID:27894322
Autor:Jaureguiberry M; Madoz LV; Giuliodori MJ; Wagener K; Prunner I; Grunert T; Ehling-Schulz M; Drillich M; de la Sota RL
Endereço:Cátedra y Servicio de Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de La Plata (FCV-UNLP), B1900AVW, La Plata, Argentina.
Título:Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy.
Fonte:Acta Vet Scand; 58(1):81, 2016 Nov 28.
ISSN:1751-0147
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Uterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. The objective of the present study was to evaluate whether a database built with uterine bacteria from European dairy cows could be used to identify bacteria from Argentinean cows by Fourier transformed infrared (FTIR) spectroscopy. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and then re-evaluated by FTIR spectroscopy (n = 27). RESULTS: FTIR spectroscopy identified Escherichia coli in 12 out of 14 samples and Trueperella pyogenes in 8 out of 10 samples. The agreement between the two methods was good with a Kappa coefficient of 0.73. In addition, the likelihood for bacterial growth of common uterine pathogens such as E. coli and T. pyogenes tended to increase with VD score. The odds for a positive result to E. coli or T. pyogenes was 1.88 times higher in cows with fetid VD than in herdmates with clear normal VD. CONCLUSIONS: We conclude that the presence of E. coli and T. pyogenes in uterine samples from Argentinean dairy cows can be detected with FTIR with the use of a database built with uterine bacteria from European dairy cows. Future studies are needed to determine if FTIR can be used as an alternative to routine bacteriological testing methods.
Tipo de publicação: JOURNAL ARTICLE


  10 / 626 MEDLINE  
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PMID:27759406
Autor:ElSalhy M; Söderling E; Honkala E; Fontana M; Flannagan S; Kokaras A; Paster BJ; Varghese A; Honkala S
Endereço:Faculty of Dentistry, Kuwait University, Kuwait - Institute of Dentistry, University of Turku, Finland.
Título:Salivary microbiota and caries occurrence in Mutans Streptococci-positive school children.
Fonte:Eur J Paediatr Dent; 17(3):188-192, 2016 Sep.
ISSN:1591-996X
País de publicação:Italy
Idioma:eng
Resumo:AIM: To compare the composition of the salivary microbiota in caries-affected vs. caries-free mutans streptococci (MS)- positive children with mixed dentition. MATERIALS AND METHODS: Twenty eight healthy, 11-12-year-old schoolchildren with high MS counts (>10⊃5 CFU/mL) were included in this study. The children were screened with the Dentocult SM Strip Mutans test (Orion Diagnostica, Espoo, Finland) and examined using the International Caries Detection and Assessment System (ICDAS). The microbial composition of the saliva was assessed using the Human Oral Microbe Identification Microarray (HOMIM). Microbial differences between caries-affected (n=18) and caries-free children (n=10) were compared by Mann-Whitney analysis. RESULTS: The microbiota of the caries-affected vs. caries-free children was rather similar. Abiotrophia defectiva and Actinomyces meyeri/A. odontolyticus were significantly higher in caries-affected than in caries-free children (p=0.006, 0.046, respectively). Shuttleworthia satelles was significantly higher in caries-free compared to caries-affected children (p=0.031). A. defectiva and A. meyeri/A. odontolyticus correlated positively with caries severity measured by ICDAS Caries Index (p = 0.494, 0.454, 0.400 respectively) while S. satelles was negatively correlated with caries severity (p= -0.489). CONCLUSIONS: Salivary A. defectiva and A. meyeri/A. odontolyticus and are associated with caries occurrence in MS-positive children with mixed dentition.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE



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