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PMID:27343694
Autor:Abdulbaqi HR; Himratul-Aznita WH; Baharuddin NA
Endereço:Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Título:Anti-plaque effect of a synergistic combination of green tea and Salvadora persica L. against primary colonizers of dental plaque.
Fonte:Arch Oral Biol; 70:117-124, 2016 Oct.
ISSN:1879-1506
País de publicação:England
Idioma:eng
Resumo:OBJECTIVE: Green tea (Gt), leafs of Camellia sinensis var. assamica, is widely consumed as healthy beverage since thousands of years in Asian countries. Chewing sticks (miswak) of Salvadora persica L. (Sp) are traditionally used as natural brush to ensure oral health in developing countries. Both Gt and Sp extracts were reported to have anti-bacterial activity against many dental plaque bacteria. However, their combination has never been tested to have anti-bacterial and anti-adherence effect against primary dental plaque colonizers, playing an initial role in the dental plaque development, which was investigated in this study. METHODS: Two-fold serial micro-dilution method was used to measure minimal inhibitory concentration (MIC) of aqueous extracts of Gt, Sp and their combinations. Adsorption to hexadecane was used to determine the cell surface hydrophobicity (CSH) of bacterial cells. Glass beads were used to mimic the hard tissue surfaces, and were coated with saliva to develop experimental pellicles for the adhesion of the primary colonizing bacteria. RESULTS: Gt aqueous extracts exhibited better anti-plaque effect than Sp aqueous extracts. Their combination, equivalent to 1/4 and 1/2 of MIC values of Gt and Sp extracts respectively, showed synergistic anti-plaque properties with fractional inhibitory concentration (FIC) equal to 0.75. This combination was found to significantly reduce CSH (p<0.05) and lower the adherence ability (p<0.003) towards experimental pellicles. CONCLUSION: Combination between Gt and Sp aqueous extracts exhibited synergistic anti-plaque activity, and could be used as a useful active agent to produce oral health care products.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (Plant Extracts); 0 (Tea)


  2 / 242 MEDLINE  
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PMID:27316318
Autor:Gao Y; Jiang X; Lin D; Chen Y; Tong Z
Endereço:Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
Título:The Starvation Resistance and Biofilm Formation of Enterococcus faecalis in Coexistence with Candida albicans, Streptococcus gordonii, Actinomyces viscosus, or Lactobacillus acidophilus.
Fonte:J Endod; 42(8):1233-8, 2016 Aug.
ISSN:1878-3554
País de publicação:United States
Idioma:eng
Resumo:INTRODUCTION: Enterococcus faecalis is the most frequently detected species in root canal-treated teeth, and it is able to survive under starvation conditions. However, persistent periapical disease is often caused by multispecies. The aim of this study was to explore the survival of E. faecalis in starvation conditions and biofilm formation with the 4 common pathogenic species. METHODS: A dual-species model of Candida albicans, Streptococcus gordonii, Actinomyces viscosus, or Lactobacillus acidophilus in combination with E. faecalis was established and allowed to grow in phosphate-buffered saline for the examination of starvation survival. Cefuroxime sodium and vancomycin at a concentration of 100 mg/L were added into brain-heart infusion plate agar to count the 2 bacteria separately in the dual species. Scanning electron microscopy was used to observe the dual species and multiple species on the root canal dentin of bovine teeth for 48 hours. A confocal laser scanning microscope was used to show the 4 groups of dual-species biofilms on substrates with glass bottoms for 48 hours. RESULTS: E. faecalis was more resistant to starvation in coexistence with C. albicans, S. gordonii, A. viscosus, or L. acidophilus, and S. gordonii was completely inhibited in coexistence with E. faecalis. The dual-species biofilm showed that E. faecalis formed thicker and denser biofilms on the root canal dentin and glass slides in coexistence with S. gordonii and A. viscosus than C. albicans and L. acidophilus. CONCLUSIONS: The multispecies community is conducive to the resistance to starvation of E. faecalis and biofilm formation in root canals.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents)


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PMID:26838144
Autor:Bariker RH; Mandroli PS
Título:An in-vitro evaluation of antibacterial effect of Amalgomer CR and Fuji VII against bacteria causing severe early childhood caries.
Fonte:J Indian Soc Pedod Prev Dent; 34(1):23-9, 2016 Jan-Mar.
ISSN:1998-3905
País de publicação:India
Idioma:eng
Resumo:OBJECTIVE: To evaluate the antibacterial action of Amalgomer CR and Fuji VII against bacteria causing S-early childhood caries. MATERIALS AND METHODS: Antibacterial activity of Amalgomer CR and Fuji VII was assessed using the agar diffusion test in triplicate. The powder and liquid of each test material was mixed and inserted in the punched wells (6 mm × 2 mm). A composition of 0.2% chlorhexidine digluconate acted as control. The agar plates were incubated at 37°C for 24 h for Streptococcus mutans, S. salivarius, S. parasanguinis and Actinomyces viscosus, whereas Lactobacillus casei was incubated for 48 h. Sizes of the inhibition zones were calculated by subtracting the diameter of the specimen (6 mm) from the average of the three measurements of the halo. For each test material against each bacteria, 9 measurements were made (3 measurements × 3 times). Kruskal-Wallis test was done to compare the zones of inhibition of test materials against individual bacteria. Pair-wise comparison was done by Mann-Whitney U-test. RESULTS: Amalgomer CR had the most antibacterial against S. mutans (31.0 mm), followed by A. viscosus (21.87 mm), S. salivarius (13.87 mm), S. parasanguinis (10.80 mm), and L. casei (9.69 mm). Fuji VII had the most antibacterial action against S. salivarius (10.65 mm), followed by A. viscosus (9.10 mm). However, it did not inhibit the growth of S. mutans (0 mm), S. parasanguinis (0 mm), and L. casei (0 mm). CONCLUSION: Amalgomer CR and Fuji VII showed wide variation in antibacterial action against all test organisms.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Amalgomer CR); 0 (Anti-Bacterial Agents); 0 (Fuji VII); 0 (Glass Ionomer Cements); MOR84MUD8E (chlorhexidine gluconate); R4KO0DY52L (Chlorhexidine)


  4 / 242 MEDLINE  
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PMID:26598183
Autor:Wu YY; Song J; Deng J; Pan KQ; Liu XY; Xu HP
Endereço:Department of Endodontics, The Affiliated Hospital of Qingdao University Medical College. Qingdao 266003, Shandong Province, China.E-mail:waiwyy1314@126.com.
Título:[Study of antimicrobial activity of carboxymethyl chitosan zinc complex material against cariogenic bacteria].
Fonte:Shanghai Kou Qiang Yi Xue; 24(5):530-4, 2015 Oct.
ISSN:1006-7248
País de publicação:China
Idioma:chi
Resumo:PUPPOSE: To evaluate the antimicrobial activity of carboxymethyl chitosan zinc (CMCSZ) and carboxymethyl chitosan zinc-active peptide complex material (CMCSZP) on oral cariogenic bacteria. METHODS: Agar dilution method and K-B disk diffusion susceptibility agents were used to measure the antimicrobial activity of two agents against S.mutans, Lactobacillus, S.sanguinis and Actinomyces viscosus. The former method was used to measure the minimal inhibitory concentration (MIC) and latter was used to measure the inhibitory zone. The effects of pH value, temperature, light, ultraviolet and storage temperature on the active substances were investigated to determine the stability of CMCSZ and CMCSZP. SPSS17.0 software package was used for statistical analysis. RESULTS: All the bacteria were susceptible to active peptide, CMCSZ and CMCSZP with the MIC of CMCSZ/CMCSZP being 625, 1250, 1250 and 2500 mg/L, respectively. At the same concentration, the inhibitory zone of CMCSZP was significantly bigger than that of CMCSZ. Acidic conditions were conducive to increase the antimicrobial activity of CMCSZ, while the effect on CMCSZP was not significant. CMCSZ and CMCSZP exhibited good stability against light, but their antimicrobial activity gradually weakened as the bath temperature rising. In the temperature of 85 degrees centigrade, their antibacterial activity disappeared. CONCLUSIONS: CMCSZ have CMCSZP had strong antimicrobial activity against 4 kinds of cariogenic bacteria. They have good stability against light, but poor thermal stability. This study provides theoretical foundation for the application of CMCSZ/CMCSZP in prevention of cariogenic diseases.
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Anti-Bacterial Agents); 0 (carboxymethyl-chitosan); 9012-76-4 (Chitosan); J41CSQ7QDS (Zinc)


  5 / 242 MEDLINE  
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PMID:26595940
Autor:Yu Q; Lin J; Zulkarjan-Ahmat; Zhao J
Título:[Effects of Yili dark bee propolis on oral cariogenic biofilm in vitro].
Fonte:Hua Xi Kou Qiang Yi Xue Za Zhi; 33(4):343-6, 2015 Aug.
ISSN:1000-1182
País de publicação:China
Idioma:chi
Resumo:OBJECTIVE: To evaluate the effects of Yili dark bee propolis on the main cariogenic biofilm and mechanisms. METHODS: Susceptibilities to the ethanolic extract of propolis against Streptococcus mutans (S. mutans), Streptococcus sobrinus (S. sobrinus), Streptococcus sanguis (S. sanguis), Actinomyces viscosus (A. viscosus), and Actinomyces naeslundii (A. naeslundii) were analyzed by crystal violet stain method to determine the minimum biofilm eradication concentration (MBEC). The biofilm was initially cultivated for 24 h. Subsequently, the propolis groups with different concentration MBEC and initial pH 7.0 were cultured for 24 h. Moreover, the pH value was measured to evaluate the acid-producing ability of the tested plaque biofilm. The effects of propolis on the insoluble extracellular polysaccharide synthesis of S. mutans biofilm were evaluated by anthrone method. RESULTS: The MBEC of Yili propolis on S. mutans, S. sobrinus, S. sanguis, A. viscosus, and A. naeslundii were 6.25, 1.56, 3.13, 0.78, and 0.78 mg.mL-1, respectively. Propolis could decrease the ΔpH of the tested plaque biofilm, and the differences between the control and propolis groups were statistically significant (P<0.05). At MBEC, propolis could reduce the ability of S. mutans in synthesizing insoluble extracellular polysaccharides. CONCLUSION: Yili propolis demonstrate remarkable eradicative effects on the cariogenic plaque biofilm, showing inhibition of the synthesis of biofilm-produced acids and insoluble extracellular polysaccharides.
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE
Nome de substância:9009-62-5 (Propolis)


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PMID:26166513
Autor:Yuan KY; Ma R; Zhu CL; Li MY; He ZY; Zhou W
Endereço:Department of Endodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;China.E-mail:ykyong6@163.com.
Título:[The efficacy of cold-light tooth bleaching on the formation of bacteria biofilm on the enamel surfaces].
Fonte:Shanghai Kou Qiang Yi Xue; 24(3):283-7, 2015 Jun.
ISSN:1006-7248
País de publicação:China
Idioma:chi
Resumo:PURPOSE: To evaluate the efficacy of Beyond cold-light tooth bleaching on the formation of main cariogenic bacteria biofilm on enamel surfaces. METHODS: Twenty enamel discs with the size of 4 mm×4 mm×1 mm in size, were made. The enamel discs were divided into 4 groups randomly: cold-light bleaching group, bleaching gel group, cold-light group and control group. Five discs were in each group. Cold-light bleaching group was whitened 3 times with bleaching gel and cold-light, and 12 min per session. Bleaching gel was smeared on the surface of enamel in bleaching gel group for 3 times and 12 min per session. Enamel discs of cold-light group were treated with cold-light for 12 min and 3 sessions. Control group was treated without any processing. The 4 groups were incubated in mixed bacteria liquid, including Streptococcus mutans(SM), Actinomyces viscosus (Av) and Fusobacterium nucleatum (Fn), within the artificial oral cavity model. After 36 h, the samples were observed under confocal laser scanning microscopy(CLSM). The data was analyzed with SAS8.2 software package. RESULTS: The biofilms in 3 experimental groups were sparser than the control group under CLSM, and the thickness significantly decreased after treatment (P<0.05), while no significant difference was found among 3 experimental groups (P>0.05).Compared with the control group, the percentage of vital bacteria in biofilm of the experimental groups decreased significantly after treatment (P<0.001). CONCLUSIONS: Cold-light tooth bleaching can inhibit the formation of mixed bacteria biofilm, damage the structure of biofilm and reduce the number of vital bacteria. Supported by Research Fund of Ninth People's Hospital of Shanghai Jiao Tong University School of Medicine (2013-06).
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T


  7 / 242 MEDLINE  
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PMID:26107177
Autor:Zhang Y; Zheng Y; Li Y; Wang L; Bai Y; Zhao Q; Xiong X; Cheng Y; Tang Z; Deng Y; Wei S
Endereço:Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University Beijing 100081, China; 2nd Dental Center, School and Hospital of Stomatology, Peking University Beijing 100081, China.
Título:Tantalum Nitride-Decorated Titanium with Enhanced Resistance to Microbiologically Induced Corrosion and Mechanical Property for Dental Application.
Fonte:PLoS One; 10(6):e0130774, 2015.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Microbiologically induced corrosion (MIC) of metallic devices/implants in the oral region is one major cause of implant failure and metal allergy in patients. Therefore, it is crucial to develop practical approaches which can effectively prevent MIC for broad clinical applications of these materials. In the present work, tantalum nitride (TaN)-decorated titanium with promoted bio-corrosion and mechanical property was firstly developed via depositing TaN layer onto pure Ti using magnetron sputtering. The microstructure and chemical constituent of TaN coatings were characterized, and were found to consist of a hard fcc-TaN outer layer. Besides, the addition of TaN coatings greatly increased the hardness and modulus of pristine Ti from 2.54 ± 0.20 to 29.88 ± 2.59 GPa, and from 107.19 ± 6.98 to 295.46 ± 19.36 GPa, respectively. Potentiodynamic polarization and electrochemical impedance spectroscopy studies indicated that TaN coating exhibited higher MIC resistance in comparison to bare Ti and TiN-coated coating in two bacteria-containing artificial saliva solutions. Moreover, the biofilm experiment showed that the TaN-decorated Ti sample possessed good antibacterial performance. The SEM and XPS results after biofilm removal demonstrated that TaN film remained its integrity and stability, while TiN layer detached from Ti surface in the bio-corrosion tests, demonstrating the anti-MIC behavior and the strong binding property of TaN coating to Ti substrate. Considering all these results, TaN-decorated Ti material exhibits the optimal comprehensive performance and holds great potential as implant material for dental applications.
Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Coated Materials, Biocompatible); 0 (Dental Implants); 0 (Dental Materials); 0 (Nitrogen Compounds); 6424HBN274 (Tantalum); D1JT611TNE (Titanium)


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PMID:25776514
Autor:Sosroseno W; Herminajeng E; Bird P
Endereço:AIMST University, Faculty of Dentistry, Semeling 08100 Bedong, Kedah, Malaysia. Electronic address: wihas@aimst.edu.my.
Título:The effect of immune status, age and genetic background on induction of oral tolerance to Actinomyces viscosus in mice.
Fonte:Biomed Pharmacother; 70:294-8, 2015 Mar.
ISSN:1950-6007
País de publicação:France
Idioma:eng
Resumo:The aim of the present study was to determine the effect of immune status, age and genetic background on the induction of oral tolerance to Actinomyces viscosus. Suppression of delayed type hypersensitivity (DTH) response and antigen-specific serum antibody levels could be induced in DBA/2 mice intragastrically and systemically immunized with A. viscocus, suggesting the induction of oral tolerance. In contrast, this immune suppression could be abrogated if the animals had been systemically immunized prior to the induction of oral tolerance with the same bacterium. Long-term systemic immunization prior to intragastric immunization with A. viscocus suppressed DTH response only. Cell transfer of this group of animals also suppressed DTH response in the donors, indicating the action of suppressor cells for inhibition of DTH response. Furthermore, oral tolerance to A. viscocus failed to occur in mice aged at 3 days and 1, 2, 4, 6 and 36 weeks old. Mice bearing H-2(d) haplotype were the most susceptible to oral tolerization, followed by H-2(b) and H-2(k). Therefore, the results of the presence study suggest that the induction of oral tolerance to A. viscosus in mice may be dependence on the immune status and genetic background but not age.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T


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PMID:25649200
Autor:Baha H; Khadir K; Hali F; Benchikhi H; Zeghwagh A; Zerouali K; Belabbes H; El Mdaghri N; Soussi MA; Marnissi F; Kadioui F
Endereço:Dermatology, Ibn Rochd CHU, route 1029, immeuble 28, numéro 6 Sidi-maarouf, Casablanca, Ain Chock 20270, Maroc. Electronic address: hasna_doc2008@hotmail.fr.
Título:[Actinomycosic mycetoma of the foot in Morocco due to Actinomycetes viscosus].
Título:Mycétome actinomycosique du pied à Actinomycetes viscosus au Maroc..
Fonte:J Mycol Med; 25(1):76-80, 2015 Mar.
ISSN:1773-0449
País de publicação:France
Idioma:fre
Resumo:We present the case of an actinomycotic mycetoma of the foot due to Actinomycetes viscosus. It evolved for nine years on the foot of a 26-year-old patient from a rural environment: Douar Inezgane (city in southern Morocco). Bacteriological study of the skin and grains confirmed the diagnosis. It showed positive bacilli on direct examination and on Gram staining and in positive culture. Histological study showed a polymorphous granulomatous inflammation without signs of malignancy with actinomycotic grains. Then we retained the diagnosis of primary cutaneous actinomycosis without visceral locations. The treatment was based on antibiotics: penicillin G by intravenous infusion for five weeks, relayed orally by amoxicillin associated with trimethoprim-sulfamethoxazole for long periods. After six months of treatment, we observed a favorable outcome with reduction of the swelling, nodules, lymphadenopathy, fistula's number and extension of time of issue of grains. The current follow up is 15 months. The primary cutaneous actinomycosis is still relevant in Morocco.
Tipo de publicação: CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 8064-90-2 (Trimethoprim, Sulfamethoxazole Drug Combination)


  10 / 242 MEDLINE  
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PMID:25033632
Autor:Houzuo G; Yao X; Xiaotian L; Ling Z
Título:[In vitro study of xylitol on the growth and acid production of Actinomyces viscosus].
Fonte:Hua Xi Kou Qiang Yi Xue Za Zhi; 32(3):217-20, 2014 Jun.
ISSN:1000-1182
País de publicação:China
Idioma:chi
Resumo:OBJECTIVE: This research aimed to study the inhibitory effect of xylitol on the growth and acid production of Actinomyces viscosus (A. viscosus). METHODS: We cultivated A. viscosus in anaerobic conditions with different concentrations (128, 64, 32, 16, 8, and 4 g x L(-1)) of xylitol brain heart infusion liquid medium and determined the minimum inhibitory concentration (MIC). Subsequently, we measured the pH value of the control group, as well as those of 1/2, 1/4, 1/8 MIC, and MIC concentration groups at 1.5, 3, 6, 12, 24, and 48 h. The Delta pH and OD550 at 2, 4, 6, 8, 10, and 12 h were calculated. We discovered that the minimum xylitol concentrations suppressed 50% and 90% A. viscosus biofilm formation (i.e., MBIC50 and MBIC90). SPSS 19.0 was used to analyze the collected data, and conclusions were drawn afterward. RESULTS: Xylitol inhibited the growth ofA. viscosus at MIC of 64 g x L(-1). After 12 h, the differences of pH value among groups were all statistically significant (P < 0.05), and Delta pH increased when the MIC concentration decreased. Except for the 1/2 MIC and MIC groups, the differences of OD550 among groups had no statistical significance (P>0.05), and OD550 also increased when the MIC concentration decreased. These results imply that the ability ofA. viscosus to grow and produce acid in 1/2 MIC and MIC conditions will be reduced with the increase in xylitol concentration. The value of MIBC50 was 64 g x L(-1), whereas the value of MIBC90 was 128 g x L(-1). This finding indicates that the xylitol medium can restrict A. viscosus biofilm formation. CONCLUSION: Xylitolcan effectively inhibit the growth, adhesion, and acid production ofA. viscosus, protecting teeth from cariogenic bacteria and preventing caries to a certain extent.
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE
Nome de substância:VCQ006KQ1E (Xylitol)



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