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  1 / 1013 MEDLINE  
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PMID:28605647
Autor:McKenzie DJ; Blasco FR; Belão TC; Killen SS; Martins ND; Taylor EW; Rantin FT
Endereço:Department of Physiological Sciences, Federal University of São Carlos, São Carlos, SP, Brazil; UMR9190 Centre for Marine Biodiversity Exploitation and Conservation, Université Montpellier, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France. Electronic address: david.mckenzie@cnrs.fr.
Título:Physiological determinants of individual variation in sensitivity to an organophosphate pesticide in Nile tilapia Oreochromis niloticus.
Fonte:Aquat Toxicol; 189:108-114, 2017 Aug.
ISSN:1879-1514
País de publicação:Netherlands
Idioma:eng
Resumo:Individual variation in sub-lethal sensitivity to the organophosphate pesticide trichlorfon was investigated in Nile tilapia, using critical swimming speed (U ) as an indicator. Tilapia exposed for 96h to 500µgl trichlorfon at 26°C (Tcfn group, n=27) showed a significant decline in mean U , compared to their own control (pre-exposure) performance in clean water (-14.5±2.3%, mean±SEM), but also compared to a Sham group (n=10) maintained for 96h in clean water. Individuals varied in their relative sensitivity to the pesticide, with the decline in U after exposure varying from 1 to 41%. The U of the Tcfn group did not recover completely after 96h in clean water, remaining 9.4±3.2% below their own control performance. The decline in performance was associated with a significant increase in net cost of aerobic swimming, of +28.4±6.5% at a sustained speed of 2bodylengthss , which translated into a significant decline in swimming efficiency (E ) of -17.6±4.0% at that speed. Within the Tcfn group, individual E was a strong positive determinant of individual U across all trials, and a strong negative determinant of individual% decline in U after pesticide exposure (P<0.001, linear mixed effect models). Trichlorfon had no effects on standard metabolic rate or active metabolic rate (AMR) but, nonetheless, individual U in all trials, and% decline in U after exposure, were strongly associated with individual AMR (positively and negatively, respectively, P<0.001). Individual U under control conditions was also a strong positive determinant of U after trichlorfon exposure (P<0.001), but not of the% decline in U performance. In conclusion, the OP pesticide impaired U performance by reducing E but individual tilapia varied widely in their relative sensitivity. Intrinsic individual physiology determined effects of the pesticide on performance and, in particular, good swimmers remained better swimmers after exposure.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Insecticides); 0 (Organophosphates); 0 (Water Pollutants, Chemical); DBF2DG4G2K (Trichlorfon)


  2 / 1013 MEDLINE  
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PMID:28541126
Autor:Li B; Ma Y; Zhang YH
Endereço:a College of Life Science , Shaanxi Normal University , Xi'an , China.
Título:Oxidative stress and hepatotoxicity in the frog, Rana chensinensis, when exposed to low doses of trichlorfon.
Fonte:J Environ Sci Health B; 52(7):476-482, 2017 Jul 03.
ISSN:1532-4109
País de publicação:England
Idioma:eng
Resumo:Trichlorfon is an organophosphate insecticide that is widely used in aquaculture and agriculture against parasitic infestations and has caused aquatic toxicity to non-target organisms. To evaluate the effects of low doses of trichlorfon on the oxidative stress and hepatotoxicity in amphibians, Chinese brown frogs (Rana chensinensis) were exposed to trichlorfon at concentrations of 0, 0.01, 0.1, and 1.0 mg/L for 2 and 4 weeks. Then, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and the content of malondialdehyde (MDA) in hepatic tissue were examined to evaluate the effects of oxidative stress and lipid peroxidation. The histopathological alternations to the liver were observed through light and transmission electron microscopy (TEM). The results showed that SOD and CAT activities were increased in the livers of frogs exposed to various concentrations of trichlorfon. The GST activity showed no significant changes at any concentration after 2 weeks of exposure, whereas there was an initial increase after exposure to 0.1 mg/L of trichlorfon at 4 weeks. The content of MDA revealed a significant decrease after exposure. Histopathological and ultrastructural studies showed that trichlorfon induced hyalinization, vacuolation, nucleus necrosis, and cellular swelling in hepatocytes. These results suggest that low doses of trichlorfon could induce oxidative stress, lipid peroxidation, and hepatic lesions in frogs, which shows that even lower, non-lethal doses of trichlorfon are potentially toxic to amphibians.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antioxidants); 0 (Insecticides); 0 (Water Pollutants, Chemical); 4Y8F71G49Q (Malondialdehyde); DBF2DG4G2K (Trichlorfon); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.5.1.18 (Glutathione Transferase)


  3 / 1013 MEDLINE  
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PMID:28143582
Autor:Cheng D; Guo Z; Riegler M; Xi Z; Liang G; Xu Y
Endereço:Department of Entomology, South China Agricultural University, Guangzhou, 510640, China.
Título:Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel).
Fonte:Microbiome; 5(1):13, 2017 Feb 01.
ISSN:2049-2618
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Symbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. Here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly Bactrocera dorsalis enhances resistance to the organophosphate insecticide trichlorphon. RESULTS: We demonstrated that the gut symbiont Citrobacter sp. (CF-BD) plays a key role in the degradation of trichlorphon. Based on a comparative genomics analysis with other Citrobacter species, phosphatase hydrolase genes were identified in CF-BD. These CF-BD genes had higher expression when trichlorphon was present. Bactrocera dorsalis inoculated with isolated CF-BD obtained higher trichlorphon resistance, while antibiotic-treated flies were less resistant confirming the key role of CF-BD in insecticide resistance. CONCLUSIONS: Our findings suggest that symbiont-mediated insecticide resistance can readily develop in B. dorsalis and may represent a more widely relevant insecticide resistance mechanism than previously recognized.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (Insecticides); 0 (RNA, Ribosomal, 16S); DBF2DG4G2K (Trichlorfon); EC 3.- (Hydrolases)


  4 / 1013 MEDLINE  
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PMID:27979090
Autor:Li J; Lu J; Qiao X; Xu Z
Endereço:College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China.
Título:A study on biomimetic immunoassay-capillary electrophoresis method based on molecularly imprinted polymer for determination of trace trichlorfon residue in vegetables.
Fonte:Food Chem; 221:1285-1290, 2017 Apr 15.
ISSN:0308-8146
País de publicação:England
Idioma:eng
Resumo:Pesticide residue in vegetables is a serious problem that has adverse effects on human health. In our study, we designed and synthesized a molecularly imprinted polymer that can selectively recognize trichlorfon. Using the polymer material as biomimetic antibody, we developed a biomimetic immunoassay-capillary electrophoresis method with improved sensitivity for the detection of trichlorfon. We evaluated the competitive reactions between HRP labeled trichlorfon hapten and free trichlorfon with the biomimetic antibody. Factors that affected the sensitivity of our method were tested in detail. Under optimal conditions, the limit of detection (LOD, IC ) and the sensitivity (IC ) of this method were 0.16mgL and 0.13µgL for trichlorfon. We used this method to determine the trichlorfon spiked in the kidney bean and cucumber samples with recoveries ranging from 78.8% to 103%. We also detected residual trichlorfons in the leek samples, and these results were verified by gas chromatography method.
Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE
Nome de substância:0 (Pesticide Residues); 0 (Polymers); DBF2DG4G2K (Trichlorfon)


  5 / 1013 MEDLINE  
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PMID:27939611
Autor:Fernandes LS; Emerick GL; Ferreira RS; Santos NA; Santos AC
Endereço:Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, FCFRP, USP, Ribeirão Preto, SP, Brazil.
Título:High concentration of trichlorfon (1mM) disrupts axonal cytoskeleton and decreases the expression of plasticity-related proteins in SH-SY5Y cells.
Fonte:Toxicol In Vitro; 39:84-92, 2017 Mar.
ISSN:1879-3177
País de publicação:England
Idioma:eng
Resumo:Some organophosphorus compounds (OPs) induce a neurodegenerative disorder known as organophosphate-induced delayed neuropathy (OPIDN), which is related to irreversible inhibition of neuropathy target esterase (NTE) and impairment of neurite outgrowth. The present study addresses the effects of trichlorfon, mipafox (neuropathic model) and paraoxon (non-neuropathic model) on neurite outgrowth and neuroplasticity-related proteins in retinoic-acid-stimulated SH-SY5Y cells, a cellular model widely used to study the neurotoxicity of OPs. Mipafox (20µM) decreased cellular differentiation and the expression of neurofilament 200 (NF-200), growth associated- (GAP-43) and synaptic proteins (synapsin I and synaptophysin); whereas paraoxon (300µM) induced no effect on cellular differentiation, but significant decrease of NF-200, GAP-43, synapsin I and synaptophysin as compared to controls. However, the effects of paraoxon on these proteins were significantly lower than the effects of mipafox. In conclusion, axonal cytoskeletal proteins, as well as axonal plasticity-related proteins are more effectively affected by neuropathic (mipafox) than by non-neuropathic (paraoxon) OPs, suggesting that they might play a role in the mechanism of OPIDN. At high concentration (1mM), trichlorfon induced effects similar to those of the neuropathic OP, mipafox (20µM), but also caused high inhibition of AChE. Therefore, these effects are unlikely to occur in humans at non-lethal doses of trichlorfon.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Cholinesterase Inhibitors); 0 (GAP-43 Protein); 0 (Insecticides); 0 (Synapsins); 12UHW9R67N (Isoflurophate); 24MJP5H3YN (mipafox); DBF2DG4G2K (Trichlorfon); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (neurotoxic esterase); EC 3.1.1.7 (Acetylcholinesterase); Q9CX8P80JW (Paraoxon)


  6 / 1013 MEDLINE  
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PMID:27632165
Autor:Tian J; Yu C; Xue Y; Zhao R; Wang J; Chen L
Endereço:School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan, 430079, People's Republic of China.
Título:Performance of trichlorfon degradation by a novel Bacillus tequilensis strain PA F-3 and its proposed biodegradation pathway.
Fonte:Biodegradation; 27(4-6):265-276, 2016 Nov.
ISSN:1572-9729
País de publicação:Netherlands
Idioma:eng
Resumo:The novel trichlorfon (TCF)-degrading bacterium PA F-3, identified as Bacillus tequilensis, was isolated from pesticide-polluted soils by using an effective screening and domesticating procedure. The TCF biodegradation pathways of PA F-3 were also systematically elucidated. As revealed by high-performance liquid chromatography, the TCF residues in the mineral salt medium demonstrated that PA F-3 can utilize TCF as its sole carbon source and reach the highest degradation of 71.1 % at an initial TCF concentration of 200 mg/L within 5 days. The TCF degradation conditions were optimized using response surface methodology as follows: temperature, 28 °C; inoculum amount, 4 %; and initial TCF concentration, 125 mg/L. Biodegradation treatments supplemented with exogenous carbon sources and yeast extract markedly increased the microbial dry weights and TCF-degrading performance of PA F-3, respectively. Meanwhile, five metabolic products of TCF were identified through gas chromatography/mass spectrometry, and a biodegradation pathway was proposed. Results indicated that deoxidation and dehydration (including the cleavage of the P-C phosphonate bond and the C-O bond) were the preferred metabolic reactions of TCF in this TCF-degrading bacterium.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Insecticides); DBF2DG4G2K (Trichlorfon)


  7 / 1013 MEDLINE  
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PMID:27161040
Autor:Tian J; Dong Q; Yu C; Zhao R; Wang J; Chen L
Endereço:School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University , Wuhan, Hubei 430079, Peoples' Republic of China.
Título:Biodegradation of the Organophosphate Trichlorfon and Its Major Degradation Products by a Novel Aspergillus sydowii PA F-2.
Fonte:J Agric Food Chem; 64(21):4280-7, 2016 Jun 01.
ISSN:1520-5118
País de publicação:United States
Idioma:eng
Resumo:Trichlorfon (TCF) is an important organophosphate pesticide in agriculture. However, limited information is known about the biodegradation behaviors and kinetics of this pesticide. In this study, a newly isolated fungus (PA F-2) from pesticide-polluted soils was identified as Aspergillus sydowii on the basis of the sequencing of internal transcribed spacer rDNA. This fungus degraded TCF as sole carbon, sole phosphorus, and sole carbon-phosphorus sources in a mineral salt medium (MSM). Optimal TCF degradation conditions were determined through response surface methodology, and results also revealed that 75.31% of 100 mg/L TCF was metabolized within 7 days. The degradation of TCF was accelerated, and the mycelial dry weight of PA F-2 was remarkably increased in MSM supplemented with exogenous sucrose and yeast extract. Five TCF metabolic products were identified through gas chromatography-mass spectrometry. TCF could be initially hydrolyzed to dichlorvos and then be degraded through the cleavage of the P-C bond to produce dimethyl hydrogen phosphate and chloral hydrate. These two compounds were subsequently deoxidized to produce dimethyl phosphite and trichloroethanal. These results demonstrate the biodegradation pathways of TCF and promote the potential use of PA F-2 to bioremediate TCF-contaminated environments.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Insecticides); 0 (Organophosphates); 0 (Soil Pollutants); DBF2DG4G2K (Trichlorfon)


  8 / 1013 MEDLINE  
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PMID:27048491
Autor:Chen XJ; Ren YJ; Meng ZY; Lu CL; Gu HT; Zhuang YQ
Endereço:Joint International Research Laboratory of Agriculture & Agri-Product Safety Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic China. cxj@yzu.edu.cn.
Título:Dissipation kinetics, safety evaluation, and preharvest interval assessment of trichlorfon application on rice.
Fonte:Environ Monit Assess; 188(5):266, 2016 May.
ISSN:1573-2959
País de publicação:Netherlands
Idioma:eng
Resumo:Nowadays, there is an urgent need for the investigation of the field dissipation and assessment of the preharvest interval for trichlorfon residues on rice. To protect consumers from potential health risks, this study can provide references for the safe application of trichlorfon in the rice fields. Results of the field dissipation study showed that the dissipation dynamic equations of trichlorfon were based on the first-order reaction dynamic equations and that the dissipation rates vary among rice plant, brown rice, rice bran, soil, and water. The 2-year field trials conducted in Yangzhou and Xiaogan suggested the interval of each application for trichlorfon on rice to be at least 7 days when 80 % trichlorfon SP was sprayed with a dose ranges between 80 and 160 a.i g/667 m(2). Additionally, the preharvest interval of the last application should be at least 15 days to ensure the amounts of residues below the maximum residue limits of trichlorfon on brown rice (0.1 mg/kg).
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Insecticides); 0 (Pesticide Residues); 0 (Soil); DBF2DG4G2K (Trichlorfon)


  9 / 1013 MEDLINE  
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PMID:26286108
Autor:Benavides-González F; Gomez-Flores RA; Rábago-Castro JL; Sánchez-Martínez JG; Montelongo-Alfaro IO
Endereço:Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Pedro de Alba s/n, San Nicolás de Los Garza, Nuevo León, México. Z. C. 66450.
Título:Effects of Hydrogen Peroxide and Metrifonate on Monogenean ligictaluridus floridanus on Catfish ( Ictalurus punctatus , Rafinesque) Gills.
Fonte:J Parasitol; 101(6):707-10, 2015 Dec.
ISSN:1937-2345
País de publicação:United States
Idioma:eng
Resumo:Hydrogen peroxide (H2O2) and metrifonate (Mtf) are common products used in ectoparasite infestations on fish cultures. The therapeutic efficacy of H2O2 and Mtf on a common monogenean parasite, Ligictaluridus floridanus, was evaluated in channel catfish ( Ictalurus punctatus ). In vitro trials were conducted using excised fish gills naturally infected with L. floridanus, which were immersed in H2O2 (150, 300, and 570 mg L(-1)) and Mtf (0.25, 0.5, and 0.75 mg L(-1)) solutions. The efficacy of the treatments was based on the survival time of the parasites, observed microscopically. In addition, an in vivo trial using catfish juveniles, naturally infected with L. floridanus, was also performed. One group received immersion baths of 570 mg L(-1) H2O2 (3%) during 4 min; the Mtf (90%) group received 0.5 mg L(-1) Mtf for 10 min. Treatments were done on days 3, 7, and 11 of the experiment. Results indicate that baths with Mtf do not significantly reduce the mean intensity of the parasite per gill arch, nor do they reduce the in vitro survival time of parasites during treatment; H2O2 baths at 570 mg L(-1) during 4 min were effective (P < 0.05) against adult and juvenile stages of L. floridanus. This study supports the use of H2O2 as an effective antiparasitic agent against I. punctatus .
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Anthelmintics); 0 (Anti-Infective Agents, Local); BBX060AN9V (Hydrogen Peroxide); DBF2DG4G2K (Trichlorfon)


  10 / 1013 MEDLINE  
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PMID:26036828
Autor:Yonar ME; Yonar SM; Pala A; Silici S; Saglam N
Endereço:Firat University, Fisheries Faculty, Department of Aquaculture, Elazig, Turkey.
Título:Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: ameliorative effect of propolis.
Fonte:Dis Aquat Organ; 114(3):209-16, 2015 Jun 03.
ISSN:0177-5103
País de publicação:Germany
Idioma:eng
Resumo:Trichlorfon is among the most commonly used products to treat fish parasites in aquaculture. We investigated the effectiveness of propolis in alleviating the toxicity of trichlorfon on haematological and oxidant/antioxidant parameters in carp Cyprinus carpio. Fish were exposed to sublethal concentrations (11 and 22 mg l-1) of trichlorfon, and propolis (10 mg kg-1 of fish weight) was simultaneously administered. At the end of 14 d administration, blood and tissue (liver, kidney, gill) samples were collected. Haematological changes (red and white blood cell count, haemoglobin concentration, haematocrit level and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration) were determined in the blood samples, while antioxidant parameters (malondialdehyde and reduced glutathione levels and superoxide dismutase, catalase and glutathione peroxidase activities) were evaluated in the liver, kidney and gill samples. Trichlorfon led to negative alterations in the haematological and antioxidant parameters investigated. The administration of propolis alleviated this effect and suggests that fish treated with trichlorfon improve their physiological status when fed a propolis-supplemented diet.
Tipo de publicação: CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
Nome de substância:0 (Anti-Infective Agents); 0 (Cholinesterase Inhibitors); 9009-62-5 (Propolis); DBF2DG4G2K (Trichlorfon)



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