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Pesquisa : D03.633.300.953.275.325 [Categoria DeCS]
Referências encontradas : 1995 [refinar]
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  1 / 1995 MEDLINE  
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PMID:29309430
Autor:Lilly JL; Gottipati A; Cahall CF; Agoub M; Berron BJ
Endereço:Department of Chemical and Materials Engineering, University of Kentucky, Lexington, Kentucky, United States of America.
Título:Comparison of eosin and fluorescein conjugates for the photoinitiation of cell-compatible polymer coatings.
Fonte:PLoS One; 13(1):e0190880, 2018.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Targeted photopolymerization is the basis for multiple diagnostic and cell encapsulation technologies. While eosin is used in conjunction with tertiary amines as a water-soluble photoinitiation system, eosin is not widely sold as a conjugate with antibodies and other targeting biomolecules. Here we evaluate the utility of fluorescein-labeled bioconjugates to photopolymerize targeted coatings on live cells. We show that although fluorescein conjugates absorb approximately 50% less light energy than eosin in matched photopolymerization experiments using a 530 nm LED lamp, appreciable polymer thicknesses can still be formed in cell compatible environments with fluorescein photosensitization. At low photoinitiator density, eosin allows more sensitive initiation of gelation. However at higher functionalization densities, the thickness of fluorescein polymer films begins to rival that of eosin. Commercial fluorescein-conjugated antibodies are also capable of generating conformal, protective coatings on mammalian cells with similar viability and encapsulation efficiency as eosin systems.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
Nome de substância:0 (Coated Materials, Biocompatible); 0 (Polymers); TDQ283MPCW (Eosine Yellowish-(YS)); TPY09G7XIR (Fluorescein)


  2 / 1995 MEDLINE  
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PMID:28466540
Autor:Cheli E; Roze J; Garrot T; Tagarist S; Briandet C; Girodon F
Endereço:Service d'Hématologie Biologique, Pôle Biologie, CHU de Dijon, France.
Título:Usefulness of the EMA flow cytometric test in the diagnosis of hereditary spherocytosis post-transfusion.
Fonte:Br J Haematol; 178(2):180, 2017 07.
ISSN:1365-2141
País de publicação:England
Idioma:eng
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:76296-42-9 (eosin maleimide); TDQ283MPCW (Eosine Yellowish-(YS))


  3 / 1995 MEDLINE  
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PMID:28599858
Autor:Shalan H; Kato M; Cheruzel L
Endereço:San José State University, Department of Chemistry, One Washington Square, San José, CA, United States.
Título:Keeping the spotlight on cytochrome P450.
Fonte:Biochim Biophys Acta; 1866(1):80-87, 2018 01.
ISSN:0006-3002
País de publicação:Netherlands
Idioma:eng
Resumo:This review describes the recent advances utilizing photosensitizers and visible light to harness the synthetic potential of P450 enzymes. The structures of the photosensitizers investigated to date are first presented along with their photophysical and redox properties. Functional photosensitizers range from organic and inorganic complexes to nanomaterials as well as the biological photosystem I complex. The focus is then on the three distinct approaches that have emerged for the activation of P450 enzymes. The first approach utilizes the in situ generation of reactive oxygen species entering the P450 mechanism via the peroxide shunt pathway. The other two approaches are sustained by electron injections into catalytically competent heme domains either facilitated by redox partners or through direct heme domain reduction. Achievements as well as pitfalls of each approach are briefly summarized. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
Nome de substância:0 (Cadmium Compounds); 0 (Light-Harvesting Protein Complexes); 0 (Peroxides); 0 (Photosensitizing Agents); 0 (Sulfides); 0 (Thioglycolates); 057EZR4Z7Q (cadmium sulfide); 11062-77-4 (Superoxides); 42VZT0U6YR (Heme); 7857H94KHM (2-mercaptoacetate); 9035-51-2 (Cytochrome P-450 Enzyme System); TDQ283MPCW (Eosine Yellowish-(YS))


  4 / 1995 MEDLINE  
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PMID:28470871
Autor:Misan A; Chan WY; Trott D; Hill PB
Endereço:School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia, 5371, Australia.
Título:Survival of Staphylococcus pseudintermedius in modified Romanowsky staining solutions.
Fonte:Vet Dermatol; 28(4):333-e71, 2017 Aug.
ISSN:1365-3164
País de publicação:England
Idioma:eng
Resumo:BACKGROUND: Stains that are used regularly for patient-side diagnosis to rapidly identify bacterial and fungal infections could become contaminated by common pathogens, such as Staphylococcus pseudintermedius, during slide immersion. HYPOTHESIS/OBJECTIVES: To determine whether the inoculation of S. pseudintermedius into modified Romanowsky type stains (Quick Dip ) results in viable bacterial contamination and whether this is influenced by the addition of organic debris (canine hair and skin). METHODS: A clinical isolate of S. pseudintermedius was inoculated into clean and organically contaminated Quick Dip solutions (methanol fixative, eosin, methylene blue), and positive (broth) and negative (bleach) controls. Each solution was tested for the presence of viable bacteria by counting the number of colony forming units (CFU/mL) at various time points. Solutions also were examined under high power microscopy to count the number of visible bacteria at each time point. RESULTS: Staphylococcus pseudintermedius was able to survive in the clean and contaminated Quick Dip stains for at least one hour, but by 24 h no viable bacteria remained. Survival of the bacteria was not supported in the fixative at any time point. Staphylococcus pseudintermedius remained visible under high power microscopy for up to 2 weeks in all organically contaminated solutions of the Quick Dip set. CONCLUSIONS AND CLINICAL IMPORTANCE: Staphylococcus pseudintermedius only remains viable in eosin and methylene blue for short periods of time, but the prolonged visibility of dead organisms could theoretically lead to the misdiagnosis of cytology samples.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Azure Stains); 0 (Romanowsky-Giemsa stain); TDQ283MPCW (Eosine Yellowish-(YS))


  5 / 1995 MEDLINE  
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PMID:28975283
Autor:Liu W; Liu JW; Ma DL
Endereço:Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, PR China.
Título:A Pink Nodule on a Boy's Left Cheek.
Fonte:JAMA; 318(11):1059-1060, 2017 Sep 19.
ISSN:1538-3598
País de publicação:United States
Idioma:eng
Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE
Nome de substância:0 (Fluorescent Dyes); TDQ283MPCW (Eosine Yellowish-(YS)); YKM8PY2Z55 (Hematoxylin)


  6 / 1995 MEDLINE  
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PMID:28433872
Autor:Karthik R; Govindasamy M; Chen SM; Cheng YH; Muthukrishnan P; Padmavathy S; Elangovan A
Endereço:Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao, East Road, Taipei 106, Taiwan, ROC.
Título:Biosynthesis of silver nanoparticles by using Camellia japonica leaf extract for the electrocatalytic reduction of nitrobenzene and photocatalytic degradation of Eosin-Y.
Fonte:J Photochem Photobiol B; 170:164-172, 2017 May.
ISSN:1873-2682
País de publicação:Switzerland
Idioma:eng
Resumo:In the present study, sphere-like silver nanoparticles (Ag-NPs) were synthesized by using Camellia japonica leaf extract and its remediation industrial pollutants such as nitrobenzene and Eosin-Y (EY). As-prepared sphere-like Ag-NPs were characterized by various analytical and spectroscopic methods such as UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), High-resolution transmission electron microscopy (HR-TEM), Energy dispersive X-ray spectra (EDX), and the chemical constituents of the leaf extract were also analyzed by using Gas chromatography and Mass Spectroscopy (GC-MS). Fascinatingly, the as-prepared sphere-like Ag-NPs exhibits excellent electrocatalytic and photocatalytic activity for the reduction of nitrobenzene and photo-degradation of EY dye respectively. The Cyclic voltammetry (CV) and amperometric (i-t) studies realized that the electrochemical behavior of sphere-like Ag-NPs modified electrode on nitrobenzene reduction. The proposed nitrobenzene sensor exhibited appreciable wide linear response range and low detection limit of 0.05-21µM, 23-2593µM and 12nM, respectively. The Ag-NPs modified electrode showed excellent selectivity towards the nitrobenzene detection even in the presence of common metal ions and nitroaromatic containing substances. On the other hand, Ag-NPs have excellent photocatalytic activity with >97% degradation of EY dye after irradiated 60min. These results indicated that the growth of sphere-like Ag-NPs should be a proficient.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Nitrobenzenes); 0 (Plant Extracts); 3M4G523W1G (Silver); TDQ283MPCW (Eosine Yellowish-(YS))


  7 / 1995 MEDLINE  
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PMID:28429192
Autor:Caires CSA; Leal CRB; Ramos CAN; Bogo D; Lima AR; Arruda EJ; Oliveira SL; Caires ARL; Nascimento VA
Endereço:Grupo de Espectroscopia e Bioinformática Aplicados a Biodiversidade e a Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, CP 549, Campo Grande, MS, 79070-900, Brazil.
Título:Photoinactivation effect of eosin methylene blue and chlorophyllin sodium-copper against Staphylococcus aureus and Escherichia coli.
Fonte:Lasers Med Sci; 32(5):1081-1088, 2017 Jul.
ISSN:1435-604X
País de publicação:England
Idioma:eng
Resumo:The use of eosin methylene blue according to Giemsa as photosensitizer is presented for the first time in this paper. The present study evaluated the potential application of chlorophyllin sodium copper salt (CuChlNa) and eosin methylene blue according to Giemsa (EMB) as antimicrobial photosensitizers (aPS) for photodynamic inactivation (PDI) of Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. The experiments were performed using S. aureus stain ATCC 25923 and E. coli ATCC 25922 in which five aPS concentrations (0.0, 1.0, 2.5, 5.0, 10.0, and 20.0 µM for S. aureus and 0.0, 5.0, 10.0, 20.0, 40.0, and 50.0 µM for E. coli) were prepared and added in 2 mL of a saline solution containing the bacterial inoculum. After aPS incubation, the samples were divided into two groups, one kept in the dark and another submitted to the illumination. Then, the bacterial inactivation was determined 18 h after the incubation at 37 °C by counting the colony-forming units (CFU). The results revealed that both EMB and CuChlNa can be used as aPS for the photoinactivation of S. aureus, while only EMB was able to photoinactivate E. coli. Nevertheless, a more complex experimental setup was needed for photoinactivation of E. coli. The data showed that EMB and CuChlNa presented similar photoinactivation effects on S. aureus, in which bacterial growth was completely inhibited at photosensitizer (PS) concentrations over 5 µM, when samples were previously incubated for 30 min and irradiated by a light dose of 30 J cm as a result of an illumination of 1 h at 8.3 mW cm by using a red light at 625 nm with a 1 cm beam diameter and output power of 6.5 mW. In the case of E. coli, bacterial growth was completely inhibited only when combining a PS incubation period of 120 min with concentrations over 20 µM.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Chlorophyllides); 0 (Photosensitizing Agents); 1D276TYV9O (chlorophyllin); T42P99266K (Methylene Blue); TDQ283MPCW (Eosine Yellowish-(YS))


  8 / 1995 MEDLINE  
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PMID:28355298
Autor:Zarella MD; Yeoh C; Breen DE; Garcia FU
Endereço:Department of Pathology & Laboratory Medicine, Drexel University, Philadelphia, PA, United States of America.
Título:An alternative reference space for H&E color normalization.
Fonte:PLoS One; 12(3):e0174489, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Digital imaging of H&E stained slides has enabled the application of image processing to support pathology workflows. Potential applications include computer-aided diagnostics, advanced quantification tools, and innovative visualization platforms. However, the intrinsic variability of biological tissue and the vast differences in tissue preparation protocols often lead to significant image variability that can hamper the effectiveness of these computational tools. We developed an alternative representation for H&E images that operates within a space that is more amenable to many of these image processing tools. The algorithm to derive this representation operates by exploiting the correlation between color and the spatial properties of the biological structures present in most H&E images. In this way, images are transformed into a structure-centric space in which images are segregated into tissue structure channels. We demonstrate that this framework can be extended to achieve color normalization, effectively reducing inter-slide variability.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Coloring Agents); TDQ283MPCW (Eosine Yellowish-(YS)); YKM8PY2Z55 (Hematoxylin)


  9 / 1995 MEDLINE  
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PMID:28347240
Autor:Chen JM; Li Y; Xu J; Gong L; Wang LW; Liu WL; Liu J
Endereço:1 Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan, China.
Título:Computer-aided prognosis on breast cancer with hematoxylin and eosin histopathology images: A review.
Fonte:Tumour Biol; 39(3):1010428317694550, 2017 Mar.
ISSN:1423-0380
País de publicação:United States
Idioma:eng
Resumo:With the advance of digital pathology, image analysis has begun to show its advantages in information analysis of hematoxylin and eosin histopathology images. Generally, histological features in hematoxylin and eosin images are measured to evaluate tumor grade and prognosis for breast cancer. This review summarized recent works in image analysis of hematoxylin and eosin histopathology images for breast cancer prognosis. First, prognostic factors for breast cancer based on hematoxylin and eosin histopathology images were summarized. Then, usual procedures of image analysis for breast cancer prognosis were systematically reviewed, including image acquisition, image preprocessing, image detection and segmentation, and feature extraction. Finally, the prognostic value of image features and image feature-based prognostic models was evaluated. Moreover, we discussed the issues of current analysis, and some directions for future research.
Tipo de publicação: JOURNAL ARTICLE; REVIEW
Nome de substância:TDQ283MPCW (Eosine Yellowish-(YS)); YKM8PY2Z55 (Hematoxylin)


  10 / 1995 MEDLINE  
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PMID:28246608
Autor:Liu H; Zhu R; Liu C; Ma R; Wang L; Chen B; Li L; Niu J; Zhao D; Mo F; Fu M; Brömme D; Zhang D; Gao S
Endereço:Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing 100029, China.
Título:Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining.
Fonte:Biomed Res Int; 2017:9050754, 2017.
ISSN:2314-6141
País de publicação:United States
Idioma:eng
Resumo:. In routine histopathology, decalcification is an essential step for mineralized tissues. The purpose of this study is to evaluate the effects of different decalcification solutions on the morphological and antigenicity preservation in Sprague Dawley (SD) rat femurs. . Four different decalcification solutions were employed to remove the mineral substances from rat femurs, including 10% neutral buffered EDTA, 3% nitric acid, 5% nitric acid, and 8% hydrochloric acid/formic acid. Shaking and low temperature were used to process the samples. The stainings of hematoxylin-eosin (HE) and immunohistochemical (IHC) were employed to evaluate the bone morphology and antigenicity. . Different decalcification solutions may affect the quality of morphology and the staining of paraffin-embedded sections in pathological examinations. Among four decalcifying solutions, 3% nitric acid is the best decalcifying agent for HE staining. 10% neutral buffered EDTA and 5% nitric acid are the preferred decalcifying agents for IHC staining. . The current study investigated the effects of different decalcifying agents on the preservation of the bone structure and antigenicity, which will help to develop suitable protocols for the analyses of the bony tissue.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antigens); 0 (Solutions); TDQ283MPCW (Eosine Yellowish-(YS)); YKM8PY2Z55 (Hematoxylin)



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