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  1 / 432 MEDLINE  
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PMID:28873548
Autor:Ma C; Zhang W; Su Z; Bai Y
Endereço:School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China. Electronic address: mcjane2013@163.com.
Título:Resonance Rayleigh scattering method for the determination of chitosan using erythrosine B as a probe and PVA as sensitization.
Fonte:Food Chem; 239:126-131, 2018 Jan 15.
ISSN:0308-8146
País de publicação:England
Idioma:eng
Resumo:A highly sensitive resonance Rayleigh scattering (RRS) method for the determination of chitosan (CTS) was developed. In the pH 3.8 Britton-Robinson buffer solution, CTS interacted with erythrosine B (Ery B) to form an ion-association complex of [Ery B-CTS] which increased the RRS intensity of the system. With the addition of polyvinyl alcohol (PVA), the RRS intensity was significantly enhanced, and the RRS intensity of the reagent blank(I ) dramatically decreased. The enhanced RRS intensity (ΔI) was linearly proportional to the concentration of chitosan. Taking the medium molecular weight chitosan as the representative, the linear range of the method was 5.0-100.0ng/mL and the detection limit (DL) was 0.47ng/mL. The effect of the molecular weight of CTS on its accurate quantification was studied. Furthermore the dispersion and stability mechanism of PVA was discussed. Accordingly this assay was applied to detect the concentration of CTS in health products with satisfactory results.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:9002-89-5 (Polyvinyl Alcohol); 9012-76-4 (Chitosan); PN2ZH5LOQY (Erythrosine)


  2 / 432 MEDLINE  
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PMID:28137461
Autor:Kuo CT; Chen YL; Hsu WT; How SC; Cheng YH; Hsueh SS; Liu HS; Lin TH; Wu JW; Wang SS
Endereço:Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Título:Investigating the effects of erythrosine B on amyloid fibril formation derived from lysozyme.
Fonte:Int J Biol Macromol; 98:159-168, 2017 May.
ISSN:1879-0003
País de publicação:Netherlands
Idioma:eng
Resumo:Formation of amyloid fibrils has been associated with at least 30 different protein aggregation diseases. The 129-residue polypeptide hen lysozyme, which is structurally homologous to human lysozyme, has been demonstrated to exhibit amyloid fibril-forming propensity in vitro. This study is aimed at exploring the influence of erythrosine B on the in vitro amyloid fibril formation of hen lysozyme at pH 2.0 and 55°C using ThT binding assay, transmission electron microscopy, far-UV circular dichroism absorption spectroscopy, 1-anilinonaphthalene-8-sulfonic acid fluorescence spectroscopy, and synchronous fluorescence study. We found that lysozyme fibrillogenesis was dose-dependently suppressed by erythrosine B. In addition, our far-UV CD and ANS fluorescence data showed that, as compared with the untreated lysozyme control, the α-to-ß transition and exposure of hydrophobic clusters in lysozyme were reduced upon treatment with erythrosine B. Moreover, it could be inferred that the binding of erythrosine B occurred in the vicinity of the tryptophan residues. Finally, molecular docking and molecular dynamics simulations were further employed to gain some insights into the possible binding site(s) and interactions between lysozyme and erythrosine B. We believe the results obtained here may contribute to the development of potential strategies/approaches for the suppression of amyloid fibrillogenesis, which is implicated in amyloid pathology.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Amyloid); EC 3.2.1.- (hen egg lysozyme); EC 3.2.1.17 (Muramidase); PN2ZH5LOQY (Erythrosine)


  3 / 432 MEDLINE  
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PMID:28091850
Autor:de Figueiredo Freitas LS; Rossoni RD; Jorge AO; Junqueira JC
Endereço:Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, UNESP - Univ. Estadual Paulista, Avenida Francisco José Longo 777, São José dos Campos, 12245-000, São Paulo, Brazil.
Título:Repeated applications of photodynamic therapy on Candida glabrata biofilms formed in acrylic resin polymerized.
Fonte:Lasers Med Sci; 32(3):549-555, 2017 Apr.
ISSN:1435-604X
País de publicação:England
Idioma:eng
Resumo:Previous studies have been suggested that photodynamic therapy (PDT) can be used as an adjuvant treatment for denture stomatitis. In this study, we evaluated the effects of multiple sessions of PDT on Candida glabrata biofilms in specimens of polymerized acrylic resin formed after 5 days. Subsequently, four applications of PDT were performed on biofilms in 24-h intervals (days 6-9). Also, we evaluated two types of PDT, including application of laser and methylene blue or light-emitting diode (LED) and erythrosine. The control groups were treated with physiological solution. The effects of PDT on biofilm were evaluated after the first and fourth application of PDT. The biofilm analysis was performed by counting the colony-forming units. The results showed that between the days 6 and 9, the biofilms not treated by PDT had an increase of 5.53 to 6.05 log (p = 0.0271). Regarding the treatments, after one application of PDT, the biofilms decreased from 5.53 to 0.89 log. When it was done four applications, the microbial reduction ranged from 6.05 log to 0.11 log. We observed that one application of PDT with laser or LED caused a reduction of 3.36 and 4.64 compared to the control groups, respectively (p = 0.1708). When it was done four applications of PDT, the reductions achieved were 1.57 for laser and 5.94 for LED (p = 0.0001). It was concluded that repeated applications of PDT on C. glabrata biofilms showed higher antimicrobial activity compared to single application. PDT mediated by LED and erythrosine was more efficient than the PDT mediated by laser and methylene blue.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Acrylic Resins); PN2ZH5LOQY (Erythrosine); T42P99266K (Methylene Blue)


  4 / 432 MEDLINE  
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PMID:27958226
Autor:Shan L; Zheng M; Zhang Y; Qu Y; Niu T; Gu Q; Liu K; Xia X
Endereço:Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080; Shanghai Key Laboratory of Fundus Disease, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Título:Correlation of Vascular Endothelial Growth Factor Production with Photochemical Reaction-induced Retinal Edema.
Fonte:Chin Med J (Engl); 129(24):2944-2950, 2016 12 20.
ISSN:0366-6999
País de publicação:China
Idioma:eng
Resumo:BACKGROUND: Retinal edema is the major complication of retinal vein occlusion and diabetic retinopathy; it can damage visual function by influencing macular region. This study was to establish a rat retinal edema model and explore the related VEGF expression and observe the responses to anti-VEGF drugs in this model. METHODS: A rat retinal edema model was established by inducing photochemical reaction using a 532 nm laser after the intravenous injection of Erythrosin B. Immediately after the laser treatment, models were given intravitreal injections of Ranibizumab or Conbercept to inhibit VEGF expression, and the changes of retinal thickness were measured. Retinal edema was observed using fundus photography (FP), optical coherence tomography (OCT), and fluoresce in fundus angiography (FFA) at 0, 1, 2, 4, 7 and 14 days after intervention. The retinal VEGF expression was measured using enzyme-linked immunosorbent assay (ELISA) and western blotting at each time point. The rat retinal edema model was also used to verify the function of anti-VEGF polypeptide ZY1. RESULTS: Both retinal edema and vascular leakage were clearly observed at 1, 2 and 4 days after photochemical induction and the retinal thickness increased notably over the same period. The retinal VEGF expression peaked at day 1 and retina became thickening simultaneously. After the interventions, the VEGF expression of the Ranibizumab and Conbercept groups decreased at each time point compared to the edema group (26.90 ± 3.57 vs. 40.29 ± 6.68, F = 31.269 on day 1 and 22.36 ± 1.12 vs. 29.92 ± 0.93 F = 163.789 on day 2, both P < 0.01); the mean RT (278 ± 4 vs. 288 ± 3, F = 134.190 on day 1 and 274 ± 7 vs. 284 ± 6, F = 64.367 on day 2, both P < 0.05) and vascular leakage in these groups also decreased. The same results were observed in the ZY1 group, particularly at day 2 (P < 0.05). CONCLUSIONS: This retinal edema model induced by a photochemical reaction is reliable and repeatable. Induced edema increases expression of VEGF. This model can be used to test new drugs.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Angiogenesis Inhibitors); 0 (Recombinant Fusion Proteins); 0 (Vascular Endothelial Growth Factor A); 1P05PW62F3 (KH902 fusion protein); PN2ZH5LOQY (Erythrosine); ZL1R02VT79 (Ranibizumab)


  5 / 432 MEDLINE  
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PMID:27667525
Autor:Ishiyama K; Nakamura K; Kanno T; Niwano Y
Endereço:Tohoku University Graduate School of Dentistry.
Título:Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm.
Fonte:Biocontrol Sci; 21(3):187-91, 2016.
ISSN:1884-0205
País de publicação:Japan
Idioma:eng
Resumo:Our previous study revealed that the photo-irradiation of rose bengal, erythrosine, and phloxine, xanthene photosensitizers, used as dental plaque disclosing agents, could exert bactericidal action on planktonic Streptococcus mutans via the singlet oxygen. In the present study, the photo-irradiation induced bactericidal activity of the three xanthene compounds against the experimental biofilm of S. mutans was investigated in combination with acid electrolyzed water (AcEW) and alkaline electrolyzed water (AlEW). As a result, only the photo-irradiated rose bengal in AlEW showed prominent bactericidal activity with a >3-log reduction of the viable bacterial count. Since our previous study showed that the affinity of rose bengal to bacterial cells was superior to that of erythrosine and phloxine, it was speculated that AlEW damaged the extracellular matrix of the experimental biofilm, which would let the rose bengal easily be bound to the bacterial cells. From these results, it is strongly suggested that rose bengal is a suitable photosensitizer for use as a plaque disclosing agent in photodynamic antimicrobial chemotherapy to treat dental plaque.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Bacterial Agents); 0 (Fluoresceins); 0 (Photosensitizing Agents); 1ZPG1ELY14 (Rose Bengal); PN2ZH5LOQY (Erythrosine); QDW0T759T6 (phloxine)


  6 / 432 MEDLINE  
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PMID:27592366
Autor:Letuta SN; Pashkevich SN; Ishemgulov AT; Lantukh YD; Alidzhanov EK; Sokabaeva SS; Bryukhanov VV
Endereço:Orenburg State University, Orenburg, Russian Federation.
Título:Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry.
Fonte:J Photochem Photobiol B; 163:232-6, 2016 Oct.
ISSN:1873-2682
País de publicação:Switzerland
Idioma:eng
Resumo:The features of delayed fluorescence (DF) and phosphorescence in erythrosine stained healthy and cancer-diseased mammary gland tissues of BYRB-line mice were investigated in vitro. Thermoactivated delayed fluorescence (TDF), triplet-triplet annihilation (TTA) and originated from singlet-triplet annihilation (STA) delayed fluorescence are investigated as competing channels of radiative relaxation of the triplet states of erythrosine. The dominant role of diffusive-mobile molecular oxygen in deactivation of triplet-excited long-live states of the dye molecules in cells is determined. The previously not described phenomenon of light quenching of DF under pulsed laser irradiation (light quenching of DF, LQDF) in stained tissue was revealed. LQDF is increased if the energy of excited pulses is rise and their sequence period is decreased. The DF depletion disappears if time interval between pulses in the series is >5s. This phenomenon is due two process competition: fast consumption of singlet oxygen by oxidation of cell organelles right after laser pulse excitation and slow diffusive recovery of oxygen concentration in the pause between the pulses. Statistically valid distinction between DF characterization as well as LQDF erythrosine extent in healthy and pathological tissues was established. The use of this phenomenon will greatly simplify the determination of radiation "dose" in photodynamic therapy (PDT) directly during the treatment session.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:PN2ZH5LOQY (Erythrosine)


  7 / 432 MEDLINE  
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Rosalen, Pedro Luiz
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PMID:27375182
Autor:Fracalossi C; Nagata JY; Pellosi DS; Terada RS; Hioka N; Baesso ML; Sato F; Rosalen PL; Caetano W; Fujimaki M
Endereço:Dentistry Department, State University of Maringá, Avenida Colombo, 5790 - Jardim Universitário, Maringá, PR, Cep 87020-900, Brazil.
Título:Singlet oxygen production by combining erythrosine and halogen light for photodynamic inactivation of Streptococcus mutans.
Fonte:Photodiagnosis Photodyn Ther; 15:127-32, 2016 Sep.
ISSN:1873-1597
País de publicação:Netherlands
Idioma:eng
Resumo:BACKGROUND: Photodynamic inactivation of microorganisms is based on a photosensitizing substance which, in the presence of light and molecular oxygen, produces singlet oxygen, a toxic agent to microorganisms and tumor cells. This study aimed to evaluate singlet oxygen quantum yield of erythrosine solutions illuminated with a halogen light source in comparison to a LED array (control), and the photodynamic effect of erythrosine dye in association with the halogen light source on Streptococcus mutans. METHODS: Singlet oxygen quantum yield of erythrosine solutions was quantified using uric acid as a chemical-probe in an aqueous solution. The in vitro effect of the photodynamic antimicrobial activity of erythrosine in association with the halogen photopolimerizing light on Streptococcus mutans (UA 159) was assessed during one minute. Bacterial cultures treated with erythrosine alone served as negative control. RESULTS: Singlet oxygen with 24% and 2.8% degradation of uric acid in one minute and a quantum yield of 0.59 and 0.63 was obtained for the erythrosine samples illuminated with the halogen light and the LED array, respectively. The bacterial cultures with erythrosine illuminated with the halogen light presented a decreased number of CFU mL(-1) in comparison with the negative control, with minimal inhibitory concentrations between 0.312 and 0.156mgmL(-1). CONCLUSIONS: The photodynamic response of erythrosine induced by the halogen light was capable of killing S. mutans. Clinical trials should be conducted to better ascertain the use of erythrosine in association with halogen light source for the treatment of dental caries.
Tipo de publicação: JOURNAL ARTICLE; REVIEW
Nome de substância:0 (Photosensitizing Agents); 17778-80-2 (Singlet Oxygen); PN2ZH5LOQY (Erythrosine)


  8 / 432 MEDLINE  
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PMID:27351664
Autor:de Oliveira PG; Silveira E Souza AM; Novaes AB; Taba M; Messora MR; Palioto DB; Grisi MF; Tedesco AC; de Souza SL
Endereço:Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, 14040-904, Ribeirão Preto, SP, Brazil.
Título:Adjunctive effect of antimicrobial photodynamic therapy in induced periodontal disease. Animal study with histomorphometrical, immunohistochemical, and cytokine evaluation.
Fonte:Lasers Med Sci; 31(7):1275-83, 2016 Sep.
ISSN:1435-604X
País de publicação:England
Idioma:eng
Resumo:Scaling and root planing (SRP) may not always be effective in preventing periodontal disease (PD) progression. The aim of this study was to evaluate the adjunctive effect of antimicrobial photodynamic therapy (aPDT) to SRP on induced PD in rats, analyzing histomorphometrical, immunohistochemical, and immunoenzymatic parameters. Ligatures were placed around the first mandibular molars and second maxillary molars of 60 rats to induce PD. After 14 days, they were removed and the animals were divided into six groups, with nine animals each: G1 = no treatment, G2 = SRP, G3 = light-emitting diode (LED), G4 = SRP + aPDT, G5 = aPDT, and G6 = erythrosine. The animals were euthanized after 3, 7, and 15 days. There were also two control groups (n = 3): without PD (WPD) induction and with maximum PD (PD+). In the histomorphometrical analysis of linear bone loss, G4 showed a statistically significant difference from the other experimental groups after 3 and 15 days. The tartrate-resistant acid phosphatase (TRAP)-positive cell counting was significantly lower in G4 when compared to G2 and PD+ after 3 days. Immunoenzymatic assay shows the values of the ratio (RANKL/OPG × 100). The lowest value is from the WPD group, and the group that received the SRP + aPDT treatment tended to approach this value over time. After 3 days, statistically significant differences were observed between G4 and all other experimental groups, as well as versus PD+ (one-way ANOVA + Tukey's post hoc test were performed, p < 0.05). It was concluded that the adjunctive use of aPDT in combination with SRP showed the best therapeutic results in the treatment of periodontal disease in rats.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Anti-Infective Agents); 0 (Cytokines); 0 (RANK Ligand); 0 (TNFSF11 protein, human); EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase); PN2ZH5LOQY (Erythrosine)


  9 / 432 MEDLINE  
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PMID:27021211
Autor:Lee J; Kwon I; Jang SS; Cho AE
Endereço:Department of Bioinformatics, Korea University, Sejong, Korea.
Título:Investigation of the effect of erythrosine B on amyloid beta peptide using molecular modeling.
Fonte:J Mol Model; 22(4):92, 2016 Apr.
ISSN:0948-5023
País de publicação:Germany
Idioma:eng
Resumo:Neurotoxic plaques composed of 39 to 42 residue-long amyloid beta peptides (Aßs) are copiously present in the brains of patients with Alzheimer's disease (AD). Erythrosine B (ER), a xanthene food dye, inhibits the formation of Aß fibrils and Aß-associated cytotoxicity in vitro. Here, in an attempt to elucidate the inhibition mechanism, we performed molecular dynamics (MD) simulations to demonstrate the conformational change of Aß40 induced by ER molecules in atomistic detail. During the simulation, the ER bound to the surfaces of both N-terminus and C-terminus regions of Aß40. Our result shows that ER interacts with the aromatic side chains at the N-terminus region resulting in destabilization of the inter-chain stacking of Aß40. Moreover, the stablility of the helical structures at the residues from 13 to 16 suggests that ER disturbs conformational transition of Aß40. At the C-terminus region, the bound ER blocks water molecules and stabilizes the α-helical structure. Regardless of the number of ER molecules used, the interruption of the formation of the salt-bridge between aspartic acid 23 and lysine 28 occurred. To further validate our analysis, binding free energies of ER at each binding site were evaluated. The finding of stronger binding energy at the N-terminus region supports an inhibition mechanism induced by stacking interaction between ER and phenylalanine. These findings could aid present and future treatment studies for AD by clarifying the inhibition mechanism of ER on the conformational transition of Aß40 at the molecular level.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Amyloid beta-Peptides); 0 (Peptide Fragments); 0 (amyloid beta-protein (1-40)); 059QF0KO0R (Water); 30KYC7MIAI (Aspartic Acid); K3Z4F929H6 (Lysine); PN2ZH5LOQY (Erythrosine)


  10 / 432 MEDLINE  
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PMID:27017835
Autor:Aoki PH; Morato LF; Pavinatto FJ; Nobre TM; Constantino CJ; Oliveira ON
Endereço:IFSC, São Carlos Institute of Physics, University of São Paulo (USP) , São Carlos, SP, Brazil 13566-590.
Título:Molecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosin.
Fonte:Langmuir; 32(15):3766-73, 2016 Apr 19.
ISSN:1520-5827
País de publicação:United States
Idioma:eng
Resumo:Incorporation into cell membranes is key for the action of photosensitizers in photomedicine treatments, with hydroperoxidation as the prominent pathway of lipid oxidation. In this paper, we use Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as cell membrane models to investigate adsorption of the photosensitizer erythrosin and its effect on photoinduced lipid oxidation. From surface pressure isotherms and polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) data, erythrosin was found to adsorb mainly via electrostatic interaction with the choline in the head groups of both DOPC and DPPC. It caused larger monolayer expansion in DOPC, with possible penetration into the hydrophobic unsaturated chains, while penetration into the DPPC saturated chains was insignificant. Easier penetration is due to the less packed DOPC monolayer, in comparison to the more compact DPPC according to the monolayer compressibility data. Most importantly, light irradiation at 530 nm made the erythrosin-containing DOPC monolayer become less unstable, with a relative surface area increase of ca. 19%, in agreement with previous findings for bioadhesive giant vesicles. The relative area increase is consistent with hydroperoxidation, supporting the erythrosin penetration into the lipid chains, which favors singlet oxygen generation close to double bonds, an important requirement for photodynamic efficiency.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Phosphatidylcholines); 0 (Photosensitizing Agents); 2644-64-6 (1,2-Dipalmitoylphosphatidylcholine); EDS2L3ODLV (1,2-oleoylphosphatidylcholine); PN2ZH5LOQY (Erythrosine)



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