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  1 / 2071 MEDLINE  
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PMID:29324346
Autor:Chen HJ; Zhou XB; Wang AL; Zheng BY; Yeh CK; Huang JD
Endereço:College of Chemistry, State Key Laboratory of Photocatalysis on Energy and Environment, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou 350116, China.
Título:Synthesis and biological characterization of novel rose bengal derivatives with improved amphiphilicity for sono-photodynamic therapy.
Fonte:Eur J Med Chem; 145:86-95, 2018 Feb 10.
ISSN:1768-3254
País de publicação:France
Idioma:eng
Resumo:Sono-Photodynamic therapy (SPDT) utilizing ultrasound and light has been demonstrated that this novel approach can lower dosage resulting in reduction of the potential side effects caused by sensitizers. Recently, a new formulation of rose bengal (RB) as an intralesional injection has completed clinical trials phase II for PDT treatment of melanoma cancer. However, the inherent unfavorable pharmacological properties of RB hindered its extensive clinical development. With the aim to identify new RB derivatives (RBDs) with enhanced photodynamic and sonodynamic anticancer efficiency, a series of amphiphilic RBDs have been designed, synthesized and biological characterized. Among them, RBD4 significantly improved cellular uptake and enhanced intracellular ROS generation efficiency upon light and ultrasound irradiation, resulting in dramatically improved anticancer potency. Notably, RBD4 has a relative potency similar to sinoporphyrin sodium (DVDMS), indicating its further potential application for SPDT.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antineoplastic Agents); 0 (Reactive Oxygen Species); 1ZPG1ELY14 (Rose Bengal)


  2 / 2071 MEDLINE  
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PMID:29353702
Autor:Manoil D; Lange N; Bouillaguet S
Endereço:Endodontics Unit, Section of Dental Medicine, University of Geneva, 1, rue Michel-Servet, CH-1206 Geneva, Switzerland. Electronic address: Daniel.Manoil@unige.ch.
Título:Enzyme-mediated photoinactivation of Enterococcus faecalis using Rose Bengal-acetate.
Fonte:J Photochem Photobiol B; 179:84-90, 2018 Feb.
ISSN:1873-2682
País de publicação:Switzerland
Idioma:eng
Resumo:Rose Bengal-acetate (RB-Ac) is a pro-photosensitizer claimed to diffuse into target cells, where the acetate groups are hydrolyzed and the photosensitizing properties of Rose Bengal (RB) are restored. Despite promising results on tumor cells, the interaction of RB-Ac with bacteria has never been investigated. This study aimed to assess the interaction of RB-Ac with Enterococcus faecalis and to evaluate its potential use in antimicrobial photodynamic therapy (aPDT). Spectrofluorometry was used to assess the ability of E. faecalis to hydrolyze the RB-Ac compound. Fluorescence microscopy was employed to observe the distribution and to evaluate the cellular uptake of the RB produced. The antibacterial efficiency of RB-Ac-mediated aPDT was assessed by flow cytometry in combination with the LIVE/DEAD® staining. Results showed that RB-Ac was successfully hydrolyzed in the presence of E. faecalis cells. The RB produced appeared to incorporate the membrane of bacteria. Higher concentrations of RB-Ac resulted in higher incorporation of RB. The blue-light irradiation of RB-Ac-treated samples significantly reduced bacterial viability. Less than 0.01% of E. faecalis survived after incubation with 200 µM RB-Ac during 900 min and blue-light activation. The current report indicates that E. faecalis cells can hydrolyze the RB-Ac compound to produce active RB. The use of RB-Ac did not appear to allow cytoplasmic internalization of the RB produced, which rather incorporated the membrane bilayers of E. faecalis. The use of RB-Ac did not provide additional advantages over RB in terms of PS localization. Nonetheless, sufficient RB was produced and incorporated into the membranes of bacteria to elicit effective aPDT.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Photosensitizing Agents); 0 (Rose Bengal acetate); 1ZPG1ELY14 (Rose Bengal)


  3 / 2071 MEDLINE  
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PMID:29242903
Autor:Seiler TG; Engler M; Beck E; Birngruber R; Kochevar IE
Endereço:Wellman Center for Photomedicine-Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States.
Título:Interface Bonding With Corneal Crosslinking (CXL) After LASIK Ex Vivo.
Fonte:Invest Ophthalmol Vis Sci; 58(14):6292-6298, 2017 Dec 01.
ISSN:1552-5783
País de publicação:United States
Idioma:eng
Resumo:Purpose: Interface bonding with corneal crosslinking (CXL) after LASIK using two different photosensitizers was studied ex vivo. Methods: A LASIK flap was created in enucleated rabbit eyes using a femtosecond laser. After the dissection, CXL was performed to seal the interface. In one group interface CXL was performed using rose bengal and green light, whereas in a second group riboflavin and UV-A light was used. In both groups irradiance, radiant exposure, dye concentration, and imbibition time was varied. In a control group, LASIK only was performed. After the procedures, the maximal shear-force required to separate the flap from the stroma was measured. Additionally, corneal transmission spectra were recorded. Results: Optimized parameters for rose bengal/green-light bonding lead to a 2.1-fold increase in shear-force compared with untreated control eyes (P < 0.01). The optimal parameter combination was: irradiance of 180 mW/cm2 for 14 minutes (total radiant exposure 150 J/cm2), rose bengal concentration 0.1%, and an imbibition time of 2 minutes. Optimized riboflavin/UV-A light parameters were 0.5% for 2 minutes with a radiant exposure of 8.1 J/cm2 obtained by an irradiance of 30 mW/cm2 for 4.5 minutes. These optimized parameters lead to a 2-fold increase compared with untreated control eyes (P < 0.01). Optical transmission experiments suggest safety for more posterior structures. Conclusions: Based on ex-vivo results, interface bonding after LASIK using crosslinking with either rose bengal or riboflavin increases the adhesion between flap and stromal bed. In vivo trials are needed to evaluate the temporal evolution of the effect.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Cross-Linking Reagents); 0 (Photosensitizing Agents); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen); TLM2976OFR (Riboflavin)


  4 / 2071 MEDLINE  
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PMID:28700779
Autor:Gallego-Muñoz P; Ibares-Frías L; Lorenzo E; Marcos S; Peréz-Merino P; Bekesi N; Kochevar IE; Martínez-García MC
Endereço:Departamento de Biología Celular, Histología y Farmacología, GIR de Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain.
Título:Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and Histologic Study.
Fonte:Invest Ophthalmol Vis Sci; 58(9):3471-3480, 2017 Jul 01.
ISSN:1552-5783
País de publicação:United States
Idioma:eng
Resumo:Purpose: To evaluate corneal wound healing after treatment with a new collagen crosslinking protocol using rose bengal dye and green light (RGX). Methods: One cornea of 20 New Zealand rabbits was de-epithelialized (DE) in an 8-mm diameter circle and, in another group (n = 25), the DE corneas were then stained with 0.1% rose bengal for 2 minutes and exposed to green light (532 nm) for 7 minutes (RGX). The contralateral eyes without treatment acted as controls. The animals were clinically followed including fluorescein staining and pachymetry. Healing events were analyzed after euthanasia at 2, 30, and 60 days. Cell death (TUNEL assay), cell proliferation (5-bromo-2'-deoxyuridine incorporation), and cell differentiation to myofibroblasts (α-SMA labeling) were carried out. In addition, loss of keratocytes and subsequent repopulation of the corneal stroma were quantified on hematoxylin-eosin-stained sections. Results: Wound closure was slower after RGX (4.4 days) then after DE (3.3 days). Cell death was restricted to the anterior central stroma, and the cellular decrease did not differ significantly between RGX and DE corneas. Cell proliferation in the epithelium and stroma appeared at 2 days. In both DE and RGX corneas, recovery of the epithelium was complete at day 30, although cell repopulation of the stroma was not complete at 60 days. Conclusions: The healing response in corneas after RGX is very similar to that observed after DE alone, suggesting that, along with its short treatment time and limited effect on keratocytes, RGX displays good potential for clinical cornea stiffening.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Cross-Linking Reagents); 0 (Fluorescent Dyes); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen)


  5 / 2071 MEDLINE  
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PMID:28614411
Autor:Minol JP; Reinsch I; Luik M; Leferink A; Barth M; Assmann A; Lichtenberg A; Akhyari P
Endereço:Department of Cardiovascular Surgery, University Hospital, Dusseldorf, Germany.
Título:Focal induction of ROS-release to trigger local vascular degeneration.
Fonte:PLoS One; 12(6):e0179342, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Reactive oxygen species (ROS) play an important role in the process of cardiovascular degeneration. We evaluated the potential of a controlled, local induction of ROS-release by application of rose bengal (RB) and photo energy to induce atherosclerosis-like focal vascular degeneration in vivo. After injection of RB, rats fed with a pro-degenerative diet underwent focal irradiation of the abdominal aorta by a green laser (ROS group), while the controls received irradiation without RB. Aortic tissue was analyzed by histology and immunohistochemistry at 0, 2, 4, 8, 28 and 56 days (n = 5). The intimal surface topography was analyzed by scanning electron microscopy. In the ROS group, an initial thrombus formation had disappeared by day 8. Similarly, ROS-derived products displayed the highest concentrations at day 0. Relative matrix metalloproteinase (MMP) activity achieved a maximum after 8 days (ROS group vs. CONTROL GROUP: 1.60 ± 0.11 vs. 0.98 ± 0.01; p < 0.001). After 28 days, no significant differences in any aspect were found between the ROS group and the controls. However, after 56 days, the aortic tissue of ROS animals exhibited relative media-pronounced thickening (ROS vs. CONTROL: 2.15 ± 0.19 vs. 0.87 ± 0.10; p < 0.001) with focal calcification and reduced expression of alpha smooth muscle actin (aSMA). The ROS-releasing application of RB and photo energy allowed for the induction of vascular degeneration in a rodent model. This protocol may be used for the focal induction of vascular disease without systemic side effects and can thereby elucidate the role of ROS in the multifactorial processes of vessel degeneration and atherogenesis.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Actins); 0 (Phosphates); 0 (Reactive Oxygen Species); 0 (Triglycerides); 1ZPG1ELY14 (Rose Bengal); 97C5T2UQ7J (Cholesterol); EC 3.4.22.- (Caspase 3); EC 3.4.24.- (Matrix Metalloproteinases); SY7Q814VUP (Calcium)


  6 / 2071 MEDLINE  
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PMID:28442903
Autor:Zhan J; Ma Z; Wang D; Li X; Li X; Le L; Kang A; Hu P; She L; Yang F
Endereço:Department of Pharmacy, Hebei North University, Zhangjiakou, Hebei.
Título:Magnetic and pH dual-responsive mesoporous silica nanocomposites for effective and low-toxic photodynamic therapy.
Fonte:Int J Nanomedicine; 12:2733-2748, 2017.
ISSN:1178-2013
País de publicação:New Zealand
Idioma:eng
Resumo:Nonspecific targeting, large doses and phototoxicity severely hamper the clinical effect of photodynamic therapy (PDT). In this work, superparamagnetic Fe O mesoporous silica nanoparticles grafted by pH-responsive block polymer polyethylene glycol- -poly(aspartic acid) (PEG- -PAsp) were fabricated to load the model photosensitizer rose bengal (RB) in the aim of enhancing the efficiency of PDT. Compared to free RB, the nanocomposites (polyethylene glycol- -polyaspartate-modified rose bengal-loaded magnetic mesoporous silica [RB-MMSNs]) could greatly enhance the cellular uptake due to their effective endocytosis by mouse melanoma B16 cell and exhibited higher induced apoptosis although with little dark toxicity. RB-MMSNs had little dark toxicity and even much could be facilitated by magnetic field in vitro. RB-MMSNs demonstrated 10 times induced apoptosis efficiency than that of free RB at the same RB concentration, both by cell counting kit-8 (CCK-8) result and apoptosis detection. Furthermore, RB-MMSNs-mediated PDT in vivo on tumor-bearing mice showed steady physical targeting of RB-MMSNs to the tumor site; tumor volumes were significantly reduced in the magnetic field with green light irradiation. More importantly, the survival time of tumor-bearing mice treated with RB-MMSNs was much prolonged. Henceforth, polyethylene glycol- -polyaspartate-modified magnetic mesoporous silica (MMSNs) probably have great potential in clinical cancer photodynamic treatment because of their effective and low-toxic performance as photosensitizers' vesicles.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Photosensitizing Agents); 0 (polyethylene glycol-block-polyaspartic acid); 1ZPG1ELY14 (Rose Bengal); 30IQX730WE (Polyethylene Glycols); 4159-77-7 (Rose Bengal B); 7631-86-9 (Silicon Dioxide); XM0M87F357 (Ferrosoferric Oxide)


  7 / 2071 MEDLINE  
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PMID:28297026
Autor:Bekesi N; Gallego-Muñoz P; Ibarés-Frías L; Perez-Merino P; Martinez-Garcia MC; Kochevar IE; Marcos S
Endereço:Instituto de Optica, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
Título:Biomechanical Changes After In Vivo Collagen Cross-Linking With Rose Bengal-Green Light and Riboflavin-UVA.
Fonte:Invest Ophthalmol Vis Sci; 58(3):1612-1620, 2017 Mar 01.
ISSN:1552-5783
País de publicação:United States
Idioma:eng
Resumo:Purpose: To compare corneal biomechanical properties after in vivo and ex vivo cross-linking (CXL) using rose bengal-green light (RGX) or riboflavin-UVA (UVX). Methods: Corneas of 30 rabbits were treated in vivo by the two CXL modalities monolaterally (Group 1) or bilaterally (Group 2). Rabbits in Group 1 were euthanized 1 month after treatments and in Group 2 two months after treatment. Ex vivo CXL was also performed. Eyes were measured by Scheimpflug air puff corneal deformation imaging (Corvis ST) under constant IOP. Corneal deformation parameters were assessed. Inherent corneal biomechanical properties were estimated using inverse finite element modeling. Results: Peak to peak distance decreased 16% 2 months after RGX, and 4% and 20% 1 and 2 months after UVX, respectively. The equivalent Young's modulus (Eeq) increased relative to the control during the post treatment period for both RGX and UVX. The Eeq increased by factors of 3.4 (RGX) and 1.7 (UVX) 1 month and by factors of 10.7 (RGX) and 7.3 (UVX) 2 months after treatment. However, the Eeq values for ex vivo CXL were much greater than produced in vivo. The ex vivo Eeq was greater than the 1-month in vivo values by factors of 8.1 (RGX) and 9.1 (UVX) and compared with 2 month by factors of 2.5 (RGX) and 2.1 (UVX). Conclusions: These results indicate that corneal stiffness increases after CXL, and further increases as a function of time after both RGX and UVX. Also, while biomechanical properties determined after ex vivo CXL are indicative of corneal stiffening, they may not provide entirely accurate information about the responses to CXL in vivo.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Cross-Linking Reagents); 0 (Fluorescent Dyes); 0 (Photosensitizing Agents); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen); TLM2976OFR (Riboflavin)


  8 / 2071 MEDLINE  
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PMID:28281771
Autor:Chisi SL; Marageni Y; Naidoo P; Zulu G; Akol GW; Van Heerden H
Endereço:Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory. songechisi@gmail.com.
Título:An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa.
Fonte:J S Afr Vet Assoc; 88(0):e1-e7, 2017 Feb 28.
ISSN:2224-9435
País de publicação:South Africa
Idioma:eng
Resumo:The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE
Nome de substância:1ZPG1ELY14 (Rose Bengal)


  9 / 2071 MEDLINE  
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PMID:28254338
Autor:Hou B; Yang W; Dong C; Zheng B; Zhang Y; Wu J; Wang H; Chang J
Endereço:School of Life Sciences, Tianjin University, PR China.
Título:Controlled co-release of doxorubicin and reactive oxygen species for synergistic therapy by NIR remote-triggered nanoimpellers.
Fonte:Mater Sci Eng C Mater Biol Appl; 74:94-102, 2017 May 01.
ISSN:1873-0191
País de publicação:Netherlands
Idioma:eng
Resumo:How to encapsulate and transport the payload of multiple therapeutic compounds avoiding premature leakage, and simultaneously co-release them rapidly at specific lesions still remains the major concern in clinic. Herein, we designed the UCN@mSiO -(Azo+RB) (azobenzene groups and Rose Bengal) nanoimpellers, which used the multicolor-emission capability of the core-shell upconverting nanoparticles (UCNs) at a single excitation wavelength to co-release anticarcinogen doxorubicin (Dox) and reactive oxygen species (ROS) for combined chemotherapy and photodynamic therapy (PDT). The nanoimpeller was formed from UCN inner core, mesoporous silica shell, and light triggers Azo and RB molecules. The UCNs emitting UV/blue and green/red multiband light were used to activate the photoresponsive Azo and photosensitizer RB molecules; The mesoporous silica shell offered the possibilities to load anticancer drug and conjugate the light triggers; As there are strong charge interaction and hydrogen bonds between Dox and surface silanols of mesoporous silica, the azobenzene molecules worked as "gatekeeper" and "molecular stirrer" to precisely trap and propel the release of Dox under the external stimuli. The time-dependent drug release analysis, ROS production test and PDT test suggested that the nanoparticles may serve as a useful multifunctional nanoplatform for synergistic therapy and cancer diagnostic.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antibiotics, Antineoplastic); 0 (Azo Compounds); 0 (Drug Carriers); 0 (Photosensitizing Agents); 0 (Reactive Oxygen Species); 1ZPG1ELY14 (Rose Bengal); 80168379AG (Doxorubicin); F0U1H6UG5C (azobenzene)


  10 / 2071 MEDLINE  
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PMID:28230241
Autor:Foote M; Read T; Thomas J; Wagels M; Burmeister B; Smithers BM
Endereço:Department of Radiation Oncology, Princess Alexandra Hospital, Queensland Health, Brisbane, Australia.
Título:Results of a phase II, open-label, non-comparative study of intralesional PV-10 followed by radiotherapy for the treatment of in-transit or metastatic melanoma.
Fonte:J Surg Oncol; 115(7):891-897, 2017 Jun.
ISSN:1096-9098
País de publicação:United States
Idioma:eng
Resumo:BACKGROUND: In-transit and recurrent dermal or subcutaneous melanoma metastases represent a significant burden of advanced disease. Intralesional Rose Bengal can elicit tumor selective ablation and a T-cell mediated abscopal effect in untreated lesions. A subset of patients in a phase II trial setting received external beam radiotherapy to their recurrent lesions with complete or partial response and no significant acute radiation reaction. METHODS: An open-label, single-arm phase II study was performed to assess the efficacy and safety of PV-10 followed by hypofractionated radiotherapy. Patients had in-transit melanoma metastases suitable for IL therapy and radiotherapy. RESULTS: Fifteen patients were enrolled and thirteen completed both treatment components. The overall response rate was 86.6% and the clinical benefit was 93.3% on an intention to treat analysis (CR 33.3%, PR 53.3%, SD 6.7%). The median follow up duration was 19.25 months. Size of metastases (<10 mm) predicted lesion complete response (74.6%). Treatment was well tolerated with no associated grade 4 or 5 adverse events. CONCLUSIONS: The combination of PV-10 and radiotherapy resulted in lesion-specific, normal tissue-sparing, ablation of disease with minimal local or systemic adverse effects.
Tipo de publicação: CLINICAL TRIAL, PHASE II; JOURNAL ARTICLE
Nome de substância:0 (Fluorescent Dyes); 1ZPG1ELY14 (Rose Bengal)



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