Base de dados : MEDLINE
Pesquisa : D08.811.682.732.700 [Categoria DeCS]
Referências encontradas : 17480 [refinar]
Mostrando: 1 .. 10   no formato [Longo]

página 1 de 1748 ir para página                         

  1 / 17480 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29484750
Autor:Saraswati S; Alhaider AA; Abdelgadir AM
Endereço:Camel Biomedical Research Unit, College of Pharmacy and Medicine, King Saud University, Riyadh, Saudi Arabia.
Título:Costunolide suppresses an inflammatory angiogenic response in a subcutaneous murine sponge model.
Fonte:APMIS; 126(3):257-266, 2018 Mar.
ISSN:1600-0463
País de publicação:Denmark
Idioma:eng
Resumo:Costunolide is known to possess anti-inflammatory and antitumor activity, but its role in tumor angiogenesis, the key step involved in tumor growth and metastasis, and the involved molecular mechanism is still unknown. We aimed to investigate the effects of costunolide on key components of inflammatory angiogenesis in the murine cannulated sponge implant angiogenesis model. Polyester-polyurethane sponges, used as a framework for fibrovascular tissue growth, were implanted in Swiss albino mice and costunolide (5, 10 and 20 mg/kg/day) was administered for 14 days through installed cannula. The implants collected at day 14 post-implantation were processed for the assessment of hemoglobin (Hb), myeloperoxidase (MPO), N-acetylglucosaminidase (NAG) and collagen, which were used as indices for angiogenesis, neutrophil and macrophage accumulation, and extracellular matrix deposition, respectively. Relevant inflammatory, angiogenic and fibrogenic cytokines were also determined. Costunolide treatment attenuated the main components of the fibrovascular tissue, wet weight, vascularization (Hb content), macrophage recruitment (NAG activity), collagen deposition, and the levels of vascular endothelial growth factor (VEGF), interleukin (IL)-1ß, IL-6, IL-17, tumor necrosis factor (TNF)-α and transforming growth factor (TGF-ß). Regulatory function of costunolide on multiple parameters of the main components of inflammatory angiogenesis has been revealed giving insight into the potential therapeutic benefit underlying the anti-angiogenic actions of costunolide.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Angiogenesis Inhibitors); 0 (Cytokines); 0 (Hemoglobins); 0 (Polyesters); 0 (Polyurethanes); 0 (Sesquiterpenes); 4IK578SA7Z (costunolide); 9007-34-5 (Collagen); EC 1.11.1.7 (Peroxidase); EC 3.2.1.52 (Acetylglucosaminidase)


  2 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29346422
Autor:Sanfins E; Correia A; B Gunnarsson S; Vilanova M; Cedervall T
Endereço:Biochemistry and Structural Biology, Lund University, Lund, Sweden.
Título:Nanoparticle effect on neutrophil produced myeloperoxidase.
Fonte:PLoS One; 13(1):e0191445, 2018.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Nanoparticles affect the immune system as they may interact directly with immune cells and activate them. However, it is possible that nanoparticles also interact with released cytokines and immunologically active enzymes. To test this hypothesis, the activity of myeloperoxidase released from activated neutrophils was measured in the presence of nanoparticles with different chemistry and size. In high concentrations of nanoparticles, myeloperoxidase activity is decreased whereas in low concentrations of nanoparticles the activity is increased. The effect of the nanoparticles on myeloperoxidase is dependent on the total protein concentration as low concentrations of bovine serum albumin together with nanoparticles further increase the myeloperoxidase activity. The results herein show that nanoparticles affect the immune response not only at the cellular level but also on released immune effectors. In particular, they show that the nanoparticle effect on myeloperoxidase activity in the neutrophil degranulation environment is the result of an intricate interplay between the enzyme and protein concentrations in the environment and the available surface area on the nanoparticle.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:EC 1.11.1.7 (Peroxidase)


  3 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29175398
Autor:Qiu N; Wang R; Sun Y; Wang X; Jiang D; Meng Y; Zhou F
Endereço:School of Life Science, Qufu Normal University, Qufu, Shandong 273165, China.
Título:Toxic effects and mechanism of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) on Lemna minor.
Fonte:Chemosphere; 193:711-719, 2018 Feb.
ISSN:1879-1298
País de publicação:England
Idioma:eng
Resumo:To investigate the toxic effect and mechanism of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in aquatic plants, in vivo and in vitro exposure to BDE-47 were conducted. After 14-d exposure to 5-20 µg/L BDE-47, the growth of Lemna minor plants was significantly suppressed, and the chlorophyll and soluble protein contents in fronds markedly decreased. Accordingly, the photosynthetic efficiency (Fv/Fm, PI) decreased. When the thylakoid membranes isolated from healthy fronds was exposed to 5-20 mg/L BDE-47 directly in vitro for 1 h, the photosynthetic efficiency also decreased significantly. In both the in vitro (5-20 µg/L) and in vivo (5-20 mg/L) experiments, BDE-47 led to an increased plasma membrane permeability. Hence, we concluded that BDE-47 had a direct toxicity to photosynthetic membranes and plasma membranes. However, direct effects on the activities of peroxidase (POD), malate dehydrogenase (MDH) and nitroreductase (NR) were not observed by adding 5-20 mg/L BDE-47 into crude enzyme extracts. The malondialdehyde (MDA) and superoxide anion radical (O ) contents in the BDE-47 treated fronds were higher than those in the control fronds, suggesting that L. minor can not effectively relieve reactive oxygen species (ROS). The data above indicates that BDE-47 is toxic to L. minor through acting directly on biomembranes, which induces the production of ROS and thus causes remarkable oxidative damage to cells.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Halogenated Diphenyl Ethers); 0 (Reactive Oxygen Species); 0 (Water Pollutants, Chemical); 0F5N573A2Y (Ether); 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether); 1406-65-1 (Chlorophyll); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.7 (Peroxidase)


  4 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
PMID:29267505
Autor:Hai W; Ping X; Zhi-Wen Y; Chun Z
Endereço:Department of Shanghai East Hospital, Tongji University, Shanghai, China.
Título:Therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis.
Fonte:Braz J Med Biol Res; 51(2):e6812, 2017 Dec 11.
ISSN:1414-431X
País de publicação:Brazil
Idioma:eng
Resumo:Caspase recruitment domain-containing protein 9 (Card9) is located upstream of the nuclear factor kappa B (NF-κB) and p38 mitogen-activated protein kinase (MAPK) inflammatory pathways. This study investigated the therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis (SAP). SAP was induced by a retrograde infusion of 5.0% sodium taurocholate into the biliopancreatic duct of Sprague Dawley rats (n=54), which were then treated with pioglitazone. Blood and pancreatic tissues were harvested at 3, 6, and 12 h after SAP induction. Pancreatic pathological damage was evaluated by hematoxylin and eosin staining. Serum amylase, serum pro-inflammatory cytokines, and pancreatic myeloperoxidase (MPO) activities were determined by enzyme-linked immunosorbent assay. The expression of Card9 mRNA and protein in pancreatic tissues was detected by real-time polymerase chain reaction and western blotting. Pioglitazone had a therapeutic effect in treating rats with SAP by decreasing the level of amylase activity, ameliorating pancreatic histological damage, decreasing serum pro-inflammatory cytokine levels and tissue MPO activity, and downregulating the expression of NF-κB, p38MAPK, and Card9 mRNAs and proteins (P<0.05). The present study demonstrated that the inhibition of Card9 expression could reduce the severity of SAP. Card9 has a role in the pathogenic mechanism of SAP.
Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE
Nome de substância:0 (Anti-Inflammatory Agents); 0 (CARD Signaling Adaptor Proteins); 0 (Card9 protein, mouse); 0 (Cytokines); 0 (NF-kappa B); 0 (Thiazolidinediones); EC 1.11.1.7 (Peroxidase); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 3.2.1.- (Amylases); X4OV71U42S (pioglitazone)


  5 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29268246
Autor:Herraiz T; Flores A; Fernández L
Endereço:Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN), Spanish National Research Council (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain. Electronic address: tomas.herraiz@csic.es.
Título:Analysis of monoamine oxidase (MAO) enzymatic activity by high-performance liquid chromatography-diode array detection combined with an assay of oxidation with a peroxidase and its application to MAO inhibitors from foods and plants.
Fonte:J Chromatogr B Analyt Technol Biomed Life Sci; 1073:136-144, 2018 Jan 15.
ISSN:1873-376X
País de publicação:Netherlands
Idioma:eng
Resumo:Monoamine oxidase (MAO) enzymes catalyze the oxidative deamination of biogenic amines and neurotransmitters and produce ammonia, aldehydes, and hydrogen peroxide which is involved in oxidative processes. Inhibitors of MAO-A and -B isozymes are useful as antidepressants and neuroprotectants. The assays of MAO usually measure amine oxidation products or hydrogen peroxide by spectrophotometric techniques. Those assays are often compromised by interfering compounds resulting in poor results. This research describes a new method that combines in the same assay the oxidative deamination of kynuramine to 4-hydroxyquinoline analyzed by HPLC-DAD with the oxidation of tetramethylbenzidine (TMB) (or Amplex Rex) by horseradish peroxidase (HRP) in presence of hydrogen peroxide. The new method was applied to study the inhibition of human MAO-A and -B by bioactive compounds including ß-carboline alkaloids and flavonoids occurring in foods and plants. As determined by HPLC-DAD, ß-carbolines, methylene blue, kaempferol and clorgyline inhibited MAO-A and methylene blue, 5-nitroindazole, norharman and deprenyl inhibited MAO-B, and all of them inhibited the oxidation of TMB in the same extent. The flavonoids catechin and cyanidin were not inhibitors of MAO by HPLC-DAD but highly inhibited the oxidation of TMB (or Amplex Red) by peroxidase whereas quercetin and resveratrol were moderate inhibitors of MAO-A by HPLC-DAD, but inhibited the peroxidase assay in a higher level. For some phenolic compounds, using the peroxidase-coupled assay to measure MAO activity led to mistaken results. The new method permits to discern between true inhibitors of MAO from those that are antioxidants and which interfere with peroxidase assays but do not inhibit MAO. For true inhibitors of MAO, inhibition as determined by HPLC-DAD correlated well with inhibition of the oxidation of TMB and this approach can be used to assess the in vitro antioxidant activity (less hydrogen peroxide production) resulting from MAO inhibition.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antioxidants); 0 (Carbolines); 0 (Flavonoids); 0 (Monoamine Oxidase Inhibitors); 0 (Plant Extracts); 363-36-0 (Kynuramine); EC 1.11.1.7 (Peroxidase); EC 1.4.3.4 (Monoamine Oxidase)


  6 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29478632
Autor:Movafeghi A; Khataee A; Abedi M; Tarrahi R; Dadpour M; Vafaei F
Endereço:Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz 51666-16471, Iran. Electronic address: movafeghi@tabrizu.ac.ir.
Título:Effects of TiO nanoparticles on the aquatic plant Spirodela polyrrhiza: Evaluation of growth parameters, pigment contents and antioxidant enzyme activities.
Fonte:J Environ Sci (China); 64:130-138, 2018 Feb.
ISSN:1001-0742
País de publicação:Netherlands
Idioma:eng
Resumo:Plants are essential components of all ecosystems and play a critical role in environmental fate of nanoparticles. However, the toxicological impacts of nanoparticles on plants are not well documented. Titanium dioxide nanoparticles (TiO -NPs) are produced worldwide in large quantities for a wide range of purposes. In the present study, the uptake of TiO -NPs by the aquatic plant Spirodela polyrrhiza and the consequent effects on the plant were evaluated. Initially, structural and morphological characteristics of the used TiO -NPs were determined using XRD, SEM, TEM and BET techniques. As a result, an anatase structure with the average crystalline size of 8nm was confirmed for the synthesized TiO -NPs. Subsequently, entrance of TiO -NP to plant roots was verified by fluorescence microscopic images. Activity of a number of antioxidant enzymes, as well as, changes in growth parameters and photosynthetic pigment contents as physiological indices were assessed to investigate the effects of TiO -NPs on S. polyrrhiza. The increasing concentration of TiO -NPs led to the significant decrease in all of the growth parameters and changes in antioxidant enzyme activities. The activity of superoxide dismutase enhanced significantly by the increasing concentration of TiO -NPs. Enhancement of superoxide dismutase activity could be explained as promoting antioxidant system to scavenging the reactive oxygen species. In contrast, the activity of peroxidase was notably decreased in the treated plants. Reduced peroxidase activity could be attributed to either direct effect of these particles on the molecular structure of the enzyme or plant defense system damage due to reactive oxygen species.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Antioxidants); 0 (Water Pollutants, Chemical); 15FIX9V2JP (titanium dioxide); D1JT611TNE (Titanium); EC 1.11.1.7 (Peroxidase); EC 1.15.1.1 (Superoxide Dismutase)


  7 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29427649
Autor:Blagojevic V; Kovacevic-Jovanovic V; Curuvija I; Petrovic R; Vujnovic I; Vujic V; Stanojevic S
Endereço:Immunology Research Centre "Branislav Jankovic", Institute of Virology, Vaccines and Sera "Torlak", Belgrade, Serbia. Electronic address: sstanojevic@torlak.rs.
Título:Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?
Fonte:Life Sci; 197:147-157, 2018 Mar 15.
ISSN:1879-0631
País de publicação:Netherlands
Idioma:eng
Resumo:AIMS: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting autoimmunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. MAIN METHODS: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. KEY FINDINGS: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b HIS48 neutrophils, and decreased the proportion of resident (CD11b HIS48 , CD163 + CD86+) and anti-inflammatory CD68 + CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b HIS48 neutrophils in DA rats, which correlated with the sustained MPO activity. SIGNIFICANCE: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Cytokines); 31C4KY9ESH (Nitric Oxide); EC 1.11.1.7 (Peroxidase); EC 3.5.3.1 (Arginase)


  8 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29317632
Autor:Fang G; Li W; Shen X; Perez-Aguilar JM; Chong Y; Gao X; Chai Z; Chen C; Ge C; Zhou R
Endereço:School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
Título:Differential Pd-nanocrystal facets demonstrate distinct antibacterial activity against Gram-positive and Gram-negative bacteria.
Fonte:Nat Commun; 9(1):129, 2018 01 09.
ISSN:2041-1723
País de publicação:England
Idioma:eng
Resumo:Noble metal-based nanomaterials have shown promise as potential enzyme mimetics, but the facet effect and underlying molecular mechanisms are largely unknown. Herein, with a combined experimental and theoretical approach, we unveil that palladium (Pd) nanocrystals exhibit facet-dependent oxidase and peroxidase-like activities that endow them with excellent antibacterial properties via generation of reactive oxygen species. The antibacterial efficiency of Pd nanocrystals against Gram-positive bacteria is consistent with the extent of their enzyme-like activity, that is {100}-faceted Pd cubes with higher activities kill bacteria more effectively than {111}-faceted Pd octahedrons. Surprisingly, a reverse trend of antibacterial activity is observed against Gram-negative bacteria, with Pd octahedrons displaying stronger penetration into bacterial membranes than Pd nanocubes, thereby exerting higher antibacterial activity than the latter. Our findings provide a deeper understanding of facet-dependent enzyme-like activities and might advance the development of noble metal-based nanomaterials with both enhanced and targeted antibacterial activities.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Anti-Bacterial Agents); 0 (Reactive Oxygen Species); 5TWQ1V240M (Palladium); EC 1.- (Oxidoreductases); EC 1.11.1.7 (Peroxidase)


  9 / 17480 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29311464
Autor:Sato S; Tsushima M; Nakamura K; Nakamura H
Endereço:Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.
Título:[Development and Application of Catalytic Tyrosine Modification].
Fonte:Yakugaku Zasshi; 138(1):39-46, 2018.
ISSN:1347-5231
País de publicação:Japan
Idioma:jpn
Resumo:The chemical labeling of proteins with synthetic probes is a key technique used in chemical biology, protein-based therapy, and material science. Much of the chemical labeling of native proteins, however, depends on the labeling of lysine and cysteine residues. While those methods have significantly contributed to native protein labeling, alternative methods that can modify different amino acid residues are still required. Herein we report the development of a novel methodology of tyrosine labeling, inspired by the luminol chemiluminescence reaction. Tyrosine residues are often exposed on a protein's surface and are thus expected to be good targets for protein functionalization. In our studies so far, we have found that 1) hemin oxidatively activates luminol derivatives as a catalyst, 2) N-methyl luminol derivative specifically forms a covalent bond with a tyrosine residue among the 20 kinds of natural amino acid residues, and 3) the efficiency of tyrosine labeling with N-methyl luminol derivative is markedly improved by using horseradish peroxidase (HRP) as a catalyst. We were able to use molecular oxygen as an oxidant under HRP/NADH conditions. By using these methods, the functionalization of purified proteins was carried out. Because N-methyl luminol derivative is an excellent protein labeling reagent that responds to the activation of peroxidase, this new method is expected to open doors to such biological applications as the signal amplification of HRP-conjugated antibodies and the detection of protein association in combination with peroxidase-tag technology.
Tipo de publicação: JOURNAL ARTICLE; REVIEW
Nome de substância:0 (Hemeproteins); 42HK56048U (Tyrosine); 42VZT0U6YR (Heme); 5EXP385Q4F (Luminol); EC 1.11.1.7 (Peroxidase)


  10 / 17480 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
PMID:29353699
Autor:Abdel-Magied N; Ahmed AG; Shedid SM
Endereço:Radiation Biology Research, National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority, 3st Ahmed Elzomer, Nasr city, Cairo, Egypt. Electronic address: nanyabdelmagid@yahoo.com.
Título:Near-infrared heat lamp therapeutic effect on paraoxonase 1 and myeloperoxidase as potential biomarkers of redox state changes induced by γ-irradiation in albino rats.
Fonte:J Photochem Photobiol B; 179:105-112, 2018 Feb.
ISSN:1873-2682
País de publicação:Switzerland
Idioma:eng
Resumo:Infrared radiation has a potential therapeutic effect in some diseases. The aim of this study was to estimate the therapeutic role of near infrared heat lamp (NIRHL) on the variations of the activity of paraoxonase 1 (PON1) and myeloperoxidase (MPO), in relation to lipid disorders, associated with oxidative stress in rats gamma-irradiated. In addition, study the effect of the duration of NIRHL treatment. Animals were divided into six groups. The results revealed that irradiated rats treated with NIRHL 20 min/once/day showed positive modulation of PON1 and MPO linked to significant improvement of lipid disorders evidenced by lower triglycerides, low density lipoprotein cholesterol (LDL-C), oxidized low density lipoprotein cholesterol (oxLDL-C) and higher density lipoprotein cholesterol (HDL-C) as well as significant amelioration of redox state, manifested by markedly increase of glutathione (GSH) content, total antioxidant capacity (TAC) associated with a noticeable decrease of pro-inflammatory cytokines. (TNF-α, IL-1 beta and IL-6), nitric oxide (NO), nitric oxide synthase (NOs), malondialdehyde (MDA), compared to irradiated rats. The results showed also that the NIRHL treatment for 20 min/twice/day had negative effects on the previous parameters and on the behavior of rats such as itching, irritability, dyspnea and death in normal as well as, irradiated rats. In conclusion, the results in this study show that NIRHL therapy for a short time can effectively prevent the lipid disorders induced by radiation through the positive modulation mechanism of PON1 and MPO enzymes and improvement of oxidative stress.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biomarkers); 0 (Cholesterol, HDL); 0 (Cholesterol, LDL); 0 (Cytokines); 31C4KY9ESH (Nitric Oxide); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.7 (Peroxidase); EC 3.1.8.1 (Aryldialkylphosphatase); GAN16C9B8O (Glutathione); SY7Q814VUP (Calcium)



página 1 de 1748 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde