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  1 / 134 MEDLINE  
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PMID:27128442
Autor:Gutsche N; Zachgo S
Endereço:Botany, University of Osnabrück, Osnabrück, Germany.
Título:The N-Terminus of the Floral Arabidopsis TGA Transcription Factor PERIANTHIA Mediates Redox-Sensitive DNA-Binding.
Fonte:PLoS One; 11(4):e0153810, 2016.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:The Arabidopsis TGA transcription factor (TF) PERIANTHIA (PAN) regulates the formation of the floral organ primordia as revealed by the pan mutant forming an abnormal pentamerous arrangement of the outer three floral whorls. The Arabidopsis TGA bZIP TF family comprises 10 members, of which PAN and TGA9/10 control flower developmental processes and TGA1/2/5/6 participate in stress-responses. For the TGA1 protein it was shown that several cysteines can be redox-dependently modified. TGA proteins interact in the nucleus with land plant-specific glutaredoxins, which may alter their activities posttranslationally. Here, we investigated the DNA-binding of PAN to the AAGAAT motif under different redox-conditions. The AAGAAT motif is localized in the second intron of the floral homeotic regulator AGAMOUS (AG), which controls stamen and carpel development as well as floral determinacy. Whereas PAN protein binds to this regulatory cis-element under reducing conditions, the interaction is strongly reduced under oxidizing conditions in EMSA studies. The redox-sensitive DNA-binding is mediated via a special PAN N-terminus, which is not present in other Arabidopsis TGA TFs and comprises five cysteines. Two N-terminal PAN cysteines, Cys68 and Cys87, were shown to form a disulfide bridge and Cys340, localized in a C-terminal putative transactivation domain, can be S-glutathionylated. Comparative land plant analyses revealed that the AAGAAT motif exists in asterid and rosid plant species. TGA TFs with N-terminal extensions of variable length were identified in all analyzed seed plants. However, a PAN-like N-terminus exists only in the rosids and exclusively Brassicaceae homologs comprise four to five of the PAN N-terminal cysteines. Redox-dependent modifications of TGA cysteines are known to regulate the activity of stress-related TGA TFs. Here, we show that the N-terminal PAN cysteines participate in a redox-dependent control of the PAN interaction with a highly conserved regulatory AG cis-element, emphasizing the importance of redox-modifications in the regulation of flower developmental processes.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (DNA, Plant); 0 (DNA-Binding Proteins); 0 (PAN protein, Arabidopsis); 0 (Recombinant Proteins); 0 (Transcription Factors); K848JZ4886 (Cysteine)


  2 / 134 MEDLINE  
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PMID:25658099
Autor:Rodríguez-Cazorla E; Ripoll JJ; Andújar A; Bailey LJ; Martínez-Laborda A; Yanofsky MF; Vera A
Endereço:Área de Genética, Universidad Miguel Hernández, Campus de Sant Joan d'Alacant, Sant Joan d'Alacant, Alicante, Spain.
Título:K-homology nuclear ribonucleoproteins regulate floral organ identity and determinacy in arabidopsis.
Fonte:PLoS Genet; 11(2):e1004983, 2015 Feb.
ISSN:1553-7404
País de publicação:United States
Idioma:eng
Resumo:Post-transcriptional control is nowadays considered a main checking point for correct gene regulation during development, and RNA binding proteins actively participate in this process. Arabidopsis thaliana FLOWERING LOCUS WITH KH DOMAINS (FLK) and PEPPER (PEP) genes encode RNA-binding proteins that contain three K-homology (KH)-domain, the typical configuration of Poly(C)-binding ribonucleoproteins (PCBPs). We previously demonstrated that FLK and PEP interact to regulate FLOWERING LOCUS C (FLC), a central repressor of flowering time. Now we show that FLK and PEP also play an important role in the maintenance of the C-function during floral organ identity by post-transcriptionally regulating the MADS-box floral homeotic gene AGAMOUS (AG). Previous studies have indicated that the KH-domain containing protein HEN4, in concert with the CCCH-type RNA binding protein HUA1 and the RPR-type protein HUA2, facilitates maturation of the AG pre-mRNA. In this report we show that FLK and PEP genetically interact with HEN4, HUA1, and HUA2, and that the FLK and PEP proteins physically associate with HUA1 and HEN4. Taken together, these data suggest that HUA1, HEN4, PEP and FLK are components of the same post-transcriptional regulatory module that ensures normal processing of the AG pre-mRNA. Our data better delineates the roles of PEP in plant development and, for the first time, links FLK to a morphogenetic process.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (FLF protein, Arabidopsis); 0 (MADS Domain Proteins); 0 (PEPPER protein, Arabidopsis); 0 (RNA-Binding Proteins)


  3 / 134 MEDLINE  
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PMID:25288633
Autor:Engelhorn J; Moreau F; Fletcher JC; Carles CC
Endereço:Université Grenoble Alpes, UMR5168, F-38041 Grenoble, France CNRS, UMR5168, F-38054 Grenoble, France CEA, iRTSV, Laboratoire Physiologie Cellulaire et Végétale, F-38054 Grenoble, France INRA, F-38054 Grenoble, France.
Título:ULTRAPETALA1 and LEAFY pathways function independently in specifying identity and determinacy at the Arabidopsis floral meristem.
Fonte:Ann Bot; 114(7):1497-505, 2014 Nov.
ISSN:1095-8290
País de publicação:England
Idioma:eng
Resumo:BACKGROUND AND AIMS: The morphological variability of the flower in angiosperms, combined with its relatively simple structure, makes it an excellent model to study cell specification and the establishment of morphogenetic patterns. Flowers are the products of floral meristems, which are determinate structures that generate four different types of floral organs before terminating. The precise organization of the flower in whorls, each defined by the identity and number of organs it contains, is controlled by a multi-layered network involving numerous transcriptional regulators. In particular, the AGAMOUS (AG) MADS domain-containing transcription factor plays a major role in controlling floral determinacy in Arabidopsis thaliana in addition to specifying reproductive organ identity. This study aims to characterize the genetic interactions between the ULTRAPETALA1 (ULT1) and LEAFY (LFY) transcriptional regulators during flower morphogenesis, with a focus on AG regulation. METHODS: Genetic and molecular approaches were used to address the question of redundancy and reciprocal interdependency for the establishment of flower meristem initiation, identity and termination. In particular, the effects of loss of both ULT1 and LFY function were determined by analysing flower developmental phenotypes of double-mutant plants. The dependency of each factor on the other for activating developmental genes was also investigated in gain-of-function experiments. KEY RESULTS: The ULT1 and LFY pathways, while both activating AG expression in the centre of the flower meristem, functioned independently in floral meristem determinacy. Ectopic transcriptional activation by ULT1 of AG and AP3, another gene encoding a MADS domain-containing flower architect, did not depend on LFY function. Similarly, LFY did not require ULT1 function to ectopically determine floral fate. CONCLUSIONS: The results indicate that the ULT1 and LFY pathways act separately in regulating identity and determinacy at the floral meristem. In particular, they independently induce AG expression in the centre of the flower to terminate meristem activity. A model is proposed whereby these independent contributions bring about a switch at the AG locus from an inactive to an active transcriptional state at the correct time and place during flower development.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (LFY protein, Arabidopsis); 0 (Transcription Factors); 0 (ULT1 protein, Arabidopsis)


  4 / 134 MEDLINE  
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PMID:25187180
Autor:Liu X; Dinh TT; Li D; Shi B; Li Y; Cao X; Guo L; Pan Y; Jiao Y; Chen X
Endereço:Key Laboratory of Agricultural Water Resources, Hebei Laboratory of Agricultural Water-Saving, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Rd, Shijiazhuang, 050021, China; Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
Título:AUXIN RESPONSE FACTOR 3 integrates the functions of AGAMOUS and APETALA2 in floral meristem determinacy.
Fonte:Plant J; 80(4):629-41, 2014 Nov.
ISSN:1365-313X
País de publicação:England
Idioma:eng
Resumo:In Arabidopsis, AUXIN RESPONSE FACTOR 3 (ARF3) belongs to the auxin response factor (ARF) family that regulates the expression of auxin-responsive genes. ARF3 is known to function in leaf polarity specification and gynoecium patterning. In this study, we discovered a previously unknown role for ARF3 in floral meristem (FM) determinacy through the isolation and characterization of a mutant of ARF3 that enhanced the FM determinacy defects of agamous (ag)-10, a weak ag allele. Central players in FM determinacy include WUSCHEL (WUS), a gene critical for FM maintenance, and AG and APETALA2 (AP2), which regulate FM determinacy by repression and promotion of WUS expression, respectively. We showed that ARF3 confers FM determinacy through repression of WUS expression, and associates with the WUS locus in part in an AG-dependent manner. We demonstrated that ARF3 is a direct target of AP2 and partially mediates AP2's function in FM determinacy. ARF3 exhibits dynamic and complex expression patterns in floral organ primordia; altering the patterns spatially compromised FM determinacy. This study uncovered a role for ARF3 in FM determinacy and revealed relationships among genes in the genetic network governing FM determinacy.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (APETALA2 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (DNA-Binding Proteins); 0 (ETT protein, Arabidopsis); 0 (Homeodomain Proteins); 0 (Nuclear Proteins); 0 (WUSCHEL protein, Arabidopsis)


  5 / 134 MEDLINE  
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PMID:25070639
Autor:Liu X; Gao L; Dinh TT; Shi T; Li D; Wang R; Guo L; Xiao L; Chen X
Endereço:Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, California 92521 Key Laboratory of Agricultural Water Resource, Hebei Laboratory of Agricultural Water-Saving, Center for Agricultural Resources Research, Institute of Genetics and
Título:DNA topoisomerase I affects polycomb group protein-mediated epigenetic regulation and plant development by altering nucleosome distribution in Arabidopsis.
Fonte:Plant Cell; 26(7):2803-17, 2014 Jul.
ISSN:1532-298X
País de publicação:United States
Idioma:eng
Resumo:It has been perplexing that DNA topoisomerases, enzymes that release DNA supercoils, play specific roles in development. In this study, using a floral stem cell model in Arabidopsis thaliana, we uncovered a role for TOPOISOMERASE1α (TOP1α) in Polycomb Group (PcG) protein-mediated histone 3 lysine 27 trimethylation (H3K27me3) at, and transcriptional repression of, the stem cell maintenance gene WUSCHEL (WUS). We demonstrated that H3K27me3 deposition at other PcG targets also requires TOP1α. Intriguingly, the repression of some, as well as the expression of many, PcG target genes requires TOP1α. The mechanism that unifies the opposing effects of TOP1α appears to lie in its role in decreasing nucleosome density, which probably allows the binding of factors that either recruit PcG, as we demonstrated for AGAMOUS at the WUS locus, or counteract PcG-mediated regulation. Although TOP1α reduces nucleosome density at all genes, the lack of a 5' nucleosome-free region is a feature that distinguishes PcG targets from nontargets and may condition the requirement for TOP1α for their expression. This study uncovers a connection between TOP1α and PcG, which explains the specific developmental functions of TOP1α.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Histones); 0 (Homeodomain Proteins); 0 (Nucleosomes); 0 (Polycomb-Group Proteins); 0 (WUSCHEL protein, Arabidopsis); EC 5.99.1.2 (DNA Topoisomerases, Type I); EC 5.99.1.2 (TOP1alpha protein, Arabidopsis)


  6 / 134 MEDLINE  
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PMID:24496620
Autor:Mandel T; Moreau F; Kutsher Y; Fletcher JC; Carles CC; Eshed Williams L
Endereço:The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12 Rehovot 76100, Israel.
Título:The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity.
Fonte:Development; 141(4):830-41, 2014 Feb.
ISSN:1477-9129
País de publicação:England
Idioma:eng
Resumo:In plants, the shoot apical meristem (SAM) serves as a reservoir of pluripotent stem cells from which all above ground organs originate. To sustain proper growth, the SAM must maintain homeostasis between the self-renewal of pluripotent stem cells and cell recruitment for lateral organ formation. At the core of the network that regulates this homeostasis in Arabidopsis are the WUSCHEL (WUS) transcription factor specifying stem cell fate and the CLAVATA (CLV) ligand-receptor system limiting WUS expression. In this study, we identified the ERECTA (ER) pathway as a second receptor kinase signaling pathway that regulates WUS expression, and therefore shoot apical and floral meristem size, independently of the CLV pathway. We demonstrate that reduction in class III HD-ZIP and ER function together leads to a significant increase in WUS expression, resulting in extremely enlarged shoot meristems and a switch from spiral to whorled vegetative phyllotaxy. We further show that strong upregulation of WUS in the inflorescence meristem leads to ectopic expression of the AGAMOUS homeotic gene to a level that switches cell fate from floral meristem founder cell to carpel founder cell, suggesting an indirect role for ER in regulating floral meristem identity. This work illustrates the delicate balance between stem cell specification and differentiation in the meristem and shows that a shift in this balance leads to abnormal phyllotaxy and to altered reproductive cell fate.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (DNA Primers); 0 (Homeodomain Proteins); 0 (Receptors, Cell Surface); 0 (WUSCHEL protein, Arabidopsis); EC 2.7.1.- (ER protein, Arabidopsis); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)


  7 / 134 MEDLINE  
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PMID:24482483
Autor:Sun B; Looi LS; Guo S; He Z; Gan ES; Huang J; Xu Y; Wee WY; Ito T
Endereço:Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Republic of Singapore.
Título:Timing mechanism dependent on cell division is invoked by Polycomb eviction in plant stem cells.
Fonte:Science; 343(6170):1248559, 2014 Jan 31.
ISSN:1095-9203
País de publicação:United States
Idioma:eng
Resumo:Plant floral stem cells divide a limited number of times before they stop and terminally differentiate, but the mechanisms that control this timing remain unclear. The precise temporal induction of the Arabidopsis zinc finger repressor KNUCKLES (KNU) is essential for the coordinated growth and differentiation of floral stem cells. We identify an epigenetic mechanism in which the floral homeotic protein AGAMOUS (AG) induces KNU at ~2 days of delay. AG binding sites colocalize with a Polycomb response element in the KNU upstream region. AG binding to the KNU promoter causes the eviction of the Polycomb group proteins from the locus, leading to cell division-dependent induction. These analyses demonstrate that floral stem cells measure developmental timing by a division-dependent epigenetic timer triggered by Polycomb eviction.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Carrier Proteins); 0 (Polycomb-Group Proteins); 0 (Trans-Activators); 0 (knuckles protein, Arabidopsis)


  8 / 134 MEDLINE  
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PMID:24482475
Autor:Zhang X
Endereço:Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
Título:Plant science. Delayed gratification--waiting to terminate stem cell identity.
Fonte:Science; 343(6170):498-9, 2014 Jan 31.
ISSN:1095-9203
País de publicação:United States
Idioma:eng
Tipo de publicação: COMMENT; JOURNAL ARTICLE
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Carrier Proteins); 0 (Polycomb-Group Proteins); 0 (knuckles protein, Arabidopsis)


  9 / 134 MEDLINE  
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PMID:24388512
Autor:Jung JH; Lee S; Yun J; Lee M; Park CM
Endereço:Department of Chemistry, Seoul National University, Seoul 151-742, Republic of Korea.
Título:The miR172 target TOE3 represses AGAMOUS expression during Arabidopsis floral patterning.
Fonte:Plant Sci; 215-216:29-38, 2014 Feb.
ISSN:1873-2259
País de publicação:Ireland
Idioma:eng
Resumo:microRNA172 (miR172) regulates phase transition and floral patterning in Arabidopsis by repressing targets that encode the APETALA2 (AP2) and AP2-like transcription factors. The miR172-mediated repression of the AP2 gene restricts AGAMOUS (AG) expression. In addition, most miR172 targets, including AP2, redundantly act as floral repressors, and the overexpression of the target genes causes delayed flowering. However, how miR172 targets other than AP2 regulate both of the developmental processes remains unclear. Here, we demonstrate that miR172-mediated repression of the TARGET OF EAT 3 (TOE3) gene is critical for floral patterning in Arabidopsis. Transgenic plants that overexpress a miR172-resistant TOE3 gene (rTOE3-ox) exhibit indeterminate flowers with numerous stamens and carpelloid organs, which is consistent with previous observations in transgenic plants that overexpress a miR172-resistant AP2 gene. TOE3 binds to the second intron of the AG gene. Accordingly, AG expression is significantly reduced in rTOE3-ox plants. TOE3 also interacts with AP2 in the nucleus. Given the major role of AP2 in floral patterning, miR172 likely regulates TOE3 in floral patterning, at least in part via AP2. In addition, a miR156 target SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 directly activates TOE3 expression, revealing a novel signaling interaction between miR156 and miR172 in floral patterning.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (APETALA2 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Homeodomain Proteins); 0 (MicroRNAs); 0 (Nuclear Proteins); 0 (Proteins); 0 (TOE3 protein, Arabidopsis); 0 (Transcription Factors); EC 4.1.99.- (spore photoproduct lyase)


  10 / 134 MEDLINE  
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PMID:23821642
Autor:ÓMaoiléidigh DS; Wuest SE; Rae L; Raganelli A; Ryan PT; Kwasniewska K; Das P; Lohan AJ; Loftus B; Graciet E; Wellmer F
Endereço:Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland.
Título:Control of reproductive floral organ identity specification in Arabidopsis by the C function regulator AGAMOUS.
Fonte:Plant Cell; 25(7):2482-503, 2013 Jul.
ISSN:1532-298X
País de publicação:United States
Idioma:eng
Resumo:The floral organ identity factor AGAMOUS (AG) is a key regulator of Arabidopsis thaliana flower development, where it is involved in the formation of the reproductive floral organs as well as in the control of meristem determinacy. To obtain insights into how AG specifies organ fate, we determined the genes and processes acting downstream of this C function regulator during early flower development and distinguished between direct and indirect effects. To this end, we combined genome-wide localization studies, gene perturbation experiments, and computational analyses. Our results demonstrate that AG controls flower development to a large extent by controlling the expression of other genes with regulatory functions, which are involved in mediating a plethora of different developmental processes. One aspect of this function is the suppression of the leaf development program in emerging floral primordia. Using trichome initiation as an example, we demonstrate that AG inhibits an important aspect of leaf development through the direct control of key regulatory genes. A comparison of the gene expression programs controlled by AG and the B function regulators APETALA3 and PISTILLATA, respectively, showed that while they control many developmental processes in conjunction, they also have marked antagonistic, as well as independent activities.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (AGAMOUS Protein, Arabidopsis); 0 (APETALA 3 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (MADS Domain Proteins); 0 (PISTILLATA protein, Arabidopsis); 147336-22-9 (Green Fluorescent Proteins)



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