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Pesquisa : D20.147 [Categoria DeCS]
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  1 / 7839 MEDLINE  
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PMID:28459063
Autor:Wang C; Zhao J; Qiu C; Wang S; Shen Y; Du B; Ding Y; Bao X
Endereço:College of Life Sciences and Shandong Key Laboratory of Agricultural Microbiology and National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an, China.
Título:Coutilization of D-Glucose, D-Xylose, and L-Arabinose in by Coexpressing the Metabolic Pathways and Evolutionary Engineering.
Fonte:Biomed Res Int; 2017:5318232, 2017.
ISSN:2314-6141
País de publicação:United States
Idioma:eng
Resumo:Efficient and cost-effective fuel ethanol production from lignocellulosic materials requires simultaneous cofermentation of all hydrolyzed sugars, mainly including D-glucose, D-xylose, and L-arabinose. is a traditional D-glucose fermenting strain and could utilize D-xylose and L-arabinose after introducing the initial metabolic pathways. The efficiency and simultaneous coutilization of the two pentoses and D-glucose for ethanol production in still need to be optimized. Previously, we constructed an L-arabinose-utilizing BSW3AP. In this study, we further introduced the XI and XR-XDH metabolic pathways of D-xylose into BSW3AP to obtain D-glucose, D-xylose, and L-arabinose cofermenting strain. Benefits of evolutionary engineering: the resulting strain BSW4XA3 displayed a simultaneous coutilization of D-xylose and L-arabinose with similar consumption rates, and the D-glucose metabolic capacity was not decreased. After 120 h of fermentation on mixed D-glucose, D-xylose, and L-arabinose, BSW4XA3 consumed 24% more amounts of pentoses and the ethanol yield of mixed sugars was increased by 30% than that of BSW3AP. The resulting strain BSW4XA3 was a useful chassis for further enhancing the coutilization efficiency of mixed sugars for bioethanol production.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biofuels); 0 (Monosaccharides); 3K9958V90M (Ethanol)


  2 / 7839 MEDLINE  
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PMID:29367147
Autor:Aslam A; Thomas-Hall SR; Manzoor M; Jabeen F; Iqbal M; Uz Zaman Q; Schenk PM; Asif Tahir M
Endereço:Department of Environmental Science, University of Lahore, Lahore, Pakistan; Algae Biotechnology Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland 4072, Australia. Electronic address: ambreenaslam86@hotmail.com.
Título:Mixed microalgae consortia growth under higher concentration of CO from unfiltered coal fired flue gas: Fatty acid profiling and biodiesel production.
Fonte:J Photochem Photobiol B; 179:126-133, 2018 Feb.
ISSN:1873-2682
País de publicação:Switzerland
Idioma:eng
Resumo:Biodiesel is produced by transesterification of fatty acid methyl esters (FAME) from oleaginous microalgae feedstock. Biodiesel fuel properties were studied and compared with biodiesel standards. Qualitative analysis of FAME was done while cultivating mixed microalgae consortia under three concentrations of coal fired flue gas (1%, 3.0% and 5.5% CO ). Under 1% CO concentration (flue gas), the FAME content was 280.3 µg/mL, whereas the lipid content was 14.03 µg/mL/D (day). Both FAMEs and lipid contents were low at other CO concentrations (3.0 and 5.5%). However, mixed consortia in the presence of phosphate buffer and flue gas (PB + FG) showed higher saturated fatty acids (SFA) (36.28%) and unsaturated fatty acids (UFA) (63.72%) versus 5.5% CO concentration, which might be responsible for oxidative stability of biodiesel. Subsequently, higher cetane number (52) and low iodine value (136.3 gI /100 g) biodiesel produced from mixed consortia (PB + FG) under 5.5% CO along with 50 mM phosphate buffer were found in accordance with European (EN 14214) standard. Results revealed that phosphate buffer significantly enhanced the biodiesel quality, but reduced the FAME yield. This study intended to develop an integrated approach for significant improvement in biodiesel quality under surplus phosphorus by utilizing waste flue gas (as CO source) using microalgae. The CO sequestration from industrial flue gas not only reduced greenhouse gases, but may also ensure the sustainable and eco-benign production of biodiesel.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biofuels); 0 (Coal); 0 (Fatty Acids); 0 (Gases); 142M471B3J (Carbon Dioxide)


  3 / 7839 MEDLINE  
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PMID:29261252
Autor:Churasia A; Singh J; Kumar A
Título:Production of biodiesel from soybean oil biomass as renewable energy source.
Fonte:J Environ Biol; 37(6):1303-07, 2016 11.
ISSN:0254-8704
País de publicação:India
Idioma:eng
Resumo:Transesterification of vegetable oils with short-chain alcohols is used to produce biodiesel. In the present study, crude soybean oil was investigated on the basis of three principal variables, such as optimum reaction temperature (60?C), the amount of catalyst concentration from 0.5, 1.0, 1.5, 2.0 and 2.5%? and molar ratio of methanol 1:1, 1:2, 1:3, 1:4, 1:5 and 1:6, respectively affecting yield of biodiesel. Under optimum condition, at molar ratio 1:6 in the presence of 0.5% NaOH and 60°C reaction temperature and 350 rpm approximately 99.1% biodiesel yield was obtained.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Biofuels); 8001-22-7 (Soybean Oil)


  4 / 7839 MEDLINE  
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PMID:28464535
Autor:Huttanus HM; Feng X
Endereço:Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Título:Compartmentalized metabolic engineering for biochemical and biofuel production.
Fonte:Biotechnol J; 12(6), 2017 Jun.
ISSN:1860-7314
País de publicação:Germany
Idioma:eng
Resumo:Sub-cellular compartments create specialized reaction chambers in eukaryotes. These compartments provide favorable micro-environments for many metabolic processes. Recently, metabolic engineers have explored the concept of pathway compartmentalization to enhance the performance of metabolic pathways. This strategy offers many unique advantages, including (i) increased local concentrations of enzymes and substrates, (ii) accessing alternate substrate pools, (iii) separation from competing reactions, and (iv) isolation of harmful intermediates or conditions needed for the pathway. In this review, the method of localizing metabolic pathways into specific organelles as well as the benefits of pathway compartmentalization in terms of enhancing the production of value-added chemicals is discussed.
Tipo de publicação: JOURNAL ARTICLE; REVIEW
Nome de substância:0 (Biofuels)


  5 / 7839 MEDLINE  
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PMID:29304058
Autor:Lima AGVO; Oliveira RL; Silva TM; Barbosa AM; Nascimento TVC; Oliveira VDS; Ribeiro RDX; Pereira ES; Bezerra LR
Endereço:Animal Science Department, Federal University of Bahia, Salvador, Bahia, Brazil.
Título:Feeding sunflower cake from biodiesel production to Santa Ines lambs: Physicochemical composition, fatty acid profile and sensory attributes of meat.
Fonte:PLoS One; 13(1):e0188648, 2018.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:The aim of this study was to determine the effect of the inclusion of sunflower cake in the diets of lambs on meat quality. Forty male, uncastrated Santa Ines lambs with an initial average body weight of 20.9 ± 0.41 kg were used in a completely randomized design with four treatments. The lambs were fed 500 g/kg hay and 500 g/kg concentrate (corn, soybean meal, urea, ammonium sulfate and sunflower cake) in their diet, and the experimental treatments were 0, 10, 20, and 30% sunflower cake inclusion (DM basis). The inclusion of sunflower cake in the diet did not affect (P > 0.05) the content of ash and protein, overall acceptance, or sensory aroma of the lamb meat. Total lipids and moisture content of the longissimus lumborum muscle increased linearly (P ≤ 0.05) with sunflower cake inclusion. The C16:0, ΣSFA, ΣMUFA:ΣSFA ratio, Δ-9 desaturase enzyme and atherogenicity index in the longissimus lumborum muscle decreased linearly (P ≤ 0.05) with sunflower cake inclusion in lamb diets, whereas a linear increase occurred (P ≤ 0.05) in C12:0, C18:0, ΣMUFA, ΣPUFA, ΣPUFA:ΣSFA and ΣPUFA:ΣMUFA ratios, Σn-6, Σn-3, desirable fatty acids, h:H index, elongase activity, and flavor, tenderness and juiciness sensory qualities in lamb meat. Among the panelists, the highest preference (35.9%) was for meat with 30% sunflower cake inclusion in the diet. Sunflower cake up to 30% of total DM can be included in the diet of Santa Ines lambs, because lipid nutritional quality and the sensory qualities of the lamb meat increase, in addition to improvement in nutraceutical compounds, such as the CLA content, and in the AI, PUFA:SFA and PUFA:MUFA ratios, desirable fatty acids content and h:H ratio, which are beneficial to the health of consumers.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biofuels); 0 (Fatty Acids)


  6 / 7839 MEDLINE  
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PMID:27777102
Autor:Mumaw CL; Surace M; Levesque S; Kodavanti UP; Kodavanti PRS; Royland JE; Block ML
Endereço:Department of Anatomy and Cell Biology, The Stark Neuroscience Research Institute, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Título:Atypical microglial response to biodiesel exhaust in healthy and hypertensive rats.
Fonte:Neurotoxicology; 59:155-163, 2017 03.
ISSN:1872-9711
País de publicação:Netherlands
Idioma:eng
Resumo:Accumulating evidence suggests a deleterious role for urban air pollution in central nervous system (CNS) diseases and neurodevelopmental disorders. Microglia, the resident innate immune cells and sentinels in the brain, are a common source of neuroinflammation and are implicated in air pollution-induced CNS effects. While renewable energy, such as soy-based biofuel, is of increasing public interest, there is little information on how soy biofuel may affect the brain, especially in people with preexisting disease conditions. To address this, male spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats were exposed to 100% Soy-based Biodiesel Exhaust (100SBDE; 0, 50, 150 and 500µg/m ) by inhalation, 4h/day for 4 weeks (5 days/week). Ionized calcium-binding adapter molecule-1 (IBA-1) staining of microglia in the substantia nigra revealed significant changes in morphology with 100SBDE exposure in rats from both genotypes, where SHR were less sensitive. Aconitase activity was inhibited in the frontal cortex and cerebellum of WKY rats exposed to 100SBDE. No consistent changes occurred in pro-inflammatory cytokine expression, nitrated protein, or arginase1 expression in brain regions from either rat strain exposed to 100SBDE. However, while IBA-1 mRNA expression was not modified, CX3CR1 mRNA expression was lower in the striatum of 100SBDE exposed rats regardless of genotype, suggesting a downregulation of the fractalkine receptor on microglia in this brain region. Together, these data indicate that while microglia are detecting and responding to 100SBDE exposure with changes in morphology, there is reduced expression of CX3CR1 regardless of genetic background and the activation response is atypical without traditional inflammatory markers of M1 or M2 activation in the brain.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
Nome de substância:0 (Aif1 protein, rat); 0 (Antioxidants); 0 (Biofuels); 0 (Calcium-Binding Proteins); 0 (Chemokine CX3CL1); 0 (Cytokines); 0 (Microfilament Proteins); 0 (RNA, Messenger); 0 (Vehicle Emissions); 3604-79-3 (3-nitrotyrosine); 42HK56048U (Tyrosine); EC 1.14.16.2 (Tyrosine 3-Monooxygenase); EC 4.2.1.3 (Aconitate Hydratase)


  7 / 7839 MEDLINE  
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PMID:27773250
Autor:Bhangu SK; Gupta S; Ashokkumar M
Endereço:School of Chemistry, University of Melbourne, VIC 3010, Australia.
Título:Ultrasonic enhancement of lipase-catalysed transesterification for biodiesel synthesis.
Fonte:Ultrason Sonochem; 34:305-309, 2017 01.
ISSN:1873-2828
País de publicação:Netherlands
Idioma:eng
Resumo:The production of biodiesel was carried out from canola oil and methanol catalysed by lipase from Candida rugosa under different ultrasonic experimental conditions using horn (20kHz) and plate (22, 44, 98 and 300kHz) transducers. The effects of experimental conditions such as horn tip diameter, ultrasonic power, ultrasonic frequency and enzyme concentrations on biodiesel yield were investigated. The results showed that the application of ultrasound decreased the reaction time from 22-24h to 1.5h with the use of 3.5cm ultrasonic horn, an applied power of 40W, methanol to oil molar ratio of 5:1 and enzyme concentration of 0.23wt/wt% of oil. Low intensity ultrasound is efficient and a promising tool for the enzyme catalysed biodiesel synthesis as higher intensities tend to inactivate the enzyme and reduce its efficiency.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biofuels); EC 3.1.1.3 (Lipase)


  8 / 7839 MEDLINE  
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PMID:29250553
Autor:Martín-Sampedro R; López-Linares JC; Fillat Ú; Gea-Izquierdo G; Ibarra D; Castro E; Eugenio ME
Endereço:INIA-CIFOR, Ctra de la Coruña, Km 7.5, 28040 Madrid, Spain.
Título:Endophytic Fungi as Pretreatment to Enhance Enzymatic Hydrolysis of Olive Tree Pruning.
Fonte:Biomed Res Int; 2017:9727581, 2017.
ISSN:2314-6141
País de publicação:United States
Idioma:eng
Resumo:Olive tree pruning, as one of the most abundant lignocellulosic residues in Mediterranean countries, has been evaluated as a source of sugars for fuel and chemicals production. A mild acid pretreatment has been combined with a fungal pretreatment using either two endophytes ( sp. and sp.) or a saprophyte ( sp. I-62). The use of endophytes is based on the important role that some of them play during the initial stages of wood decomposition. Without acid treatment, fungal pretreatment with sp. provided a nonsignificant enhancement of 4.6% in glucose digestibility, compared to control. When a mild acid hydrolysis was carried out after fungal pretreatments, significant increases in glucose digestibility from 4.9% to 12.0% (compared to control without fungi) were observed for all fungal pretreatments, with maximum values yielded by sp. However, despite the observed digestibility boost, the total sugar yields (taking into account solid yield) were not significantly increased by the pretreatments. Nevertheless, based on these preliminary improvements in digestibility, this work proves the potential of endophytic fungi to boost the production of sugar from olive tree pruning, which would add an extra value to the bioeconomy of olive crops.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biofuels)


  9 / 7839 MEDLINE  
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PMID:29250551
Autor:Naspolini BF; Machado ACO; Cravo Junior WB; Freire DMG; Cammarota MC
Endereço:National Institute of Technology (INT-MCTIC), Av. Venezuela, No. 82, Centro, 22453-900 Rio de Janeiro, RJ, Brazil.
Título:Bioconversion of Sugarcane Vinasse into High-Added Value Products and Energy.
Fonte:Biomed Res Int; 2017:8986165, 2017.
ISSN:2314-6141
País de publicação:United States
Idioma:eng
Resumo:Vinasse, a residue from bioethanol production containing high organic matter concentration, was used as substrate in submerged fermentation of PA1 for biosurfactant production. About 2.7 g/L of rhamnolipids was obtained, with surface tension of 29.2 mN/m and critical micelle concentration of 80.3 mg/L. After separation of rhamnolipid and biomass, residual fermentation media were submitted to anaerobic biodegradation in mesophilic conditions. The residual medium derived from fermentation with vinasse diluted to 1 : 1, without addition of nitrogen, C : N 21, and for 168 h, led to 63.2% chemical oxygen demand (COD) removal and 97.6 mL CH /g COD . Compared to results obtained with fresh vinasse (73.7% COD removal and 112.4 mL CH /g COD ), it could be concluded that both processes can be integrated in order to add value to the residue and obtain energy, reducing production costs and at the same time environmental impacts related to vinasse disposal.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biofuels); 0 (Glycolipids); 0 (Surface-Active Agents); 0 (rhamnolipid); N762921K75 (Nitrogen); PDC6A3C0OX (Glycerol)


  10 / 7839 MEDLINE  
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PMID:27778473
Autor:Gihaz S; Weiser D; Dror A; Sátorhelyi P; Jerabek-Willemsen M; Poppe L; Fishman A
Endereço:Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.
Título:Creating an Efficient Methanol-Stable Biocatalyst by Protein and Immobilization Engineering Steps towards Efficient Biosynthesis of Biodiesel.
Fonte:ChemSusChem; 9(22):3161-3170, 2016 11 23.
ISSN:1864-564X
País de publicação:Germany
Idioma:eng
Resumo:Two ternary sol-gel matrices, an octyltriethoxysilane-based aliphatic matrix and a phenyltriethoxysilane (PTEOS)-based aromatic matrix, were used to immobilize a methanol-stable variant of lipase from Geobacillus stearothermophilus T6 for the synthesis of biodiesel from waste oil. Superior thermal stability of the mutant versus the wildtype in methanol was confirmed by intrinsic protein fluorescence measurements. The influence of skim milk and soluble E. coli lysate proteins as bulking and stabilizing agents in conjunction with sol-gel entrapment were investigated. E. coli lysate proteins were better stabilizing agents of the purified lipase mutant than skim milk, as evidenced by reverse engineering of the aromatic-based system. This was also shown for commercial Candida antarctica lipase B (CaLB) and Thermomyces lanuginosus lipase (TLL). Uniform, dense, and nonaggregated particles imaged by scanning electron microscopy and a small particle size of 13 µm pertaining to the system comprising PTEOS and E. coli lysate proteins correlated well with high esterification activity. Combining protein and immobilization engineering resulted in a durable biocatalyst with efficient recycling ability and high biodiesel conversion rates.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Biofuels); 0 (Enzymes, Immobilized); 0 (Escherichia coli Proteins); EC 3.1.1.3 (Lipase); Y4S76JWI15 (Methanol)



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