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Pesquisa : D20.215.113 [Categoria DeCS]
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  1 / 816 MEDLINE  
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PMID:29107194
Autor:Guimarães A; Santiago A; Teixeira JA; Venâncio A; Abrunhosa L
Endereço:CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Título:Anti-aflatoxigenic effect of organic acids produced by Lactobacillus plantarum.
Fonte:Int J Food Microbiol; 264:31-38, 2018 Jan 02.
ISSN:1879-3460
País de publicação:Netherlands
Idioma:eng
Resumo:Lactic acid bacteria (LAB), which are commonly used in the production of fermented foods, have been gaining attention for their antifungal and antimycotoxin properties. In this work, the strain Lactobacillus plantarum UM55 was selected among other LAB for inhibiting the growth of Aspergillus flavus. Further, it is shown that cell-free supernatant (CFS) of this strain inhibits the production of aflatoxins (AFLs) by 91%. This inhibition was dependent on CFS pH, increased with increasing concentrations of CFS, and was independent of fungal growth, which was inhibited only by 32%. CFS was also effective in inhibiting the growth and AFLs production in A. parasiticus, A. arachidicola, A. nomius and A. minisclerotigenes. Further, L. plantarum UM55 CFS was analysed for the presence of organic acids and the main differences compared to controls were found in the levels of lactic acid, phenyllactic acid (PLA), hydroxyphenyllactic acid (OH-PLA), and indole lactic acid (ILA). These compounds were individually tested against A. flavus, with all of the compounds showing an inhibiting effect on fungal growth and AFLs production. PLA showed the stronger effects, and the obtained IC for the inhibition of growth and AFLs was of 11.9 and 0.87mg/mL, respectively. AFLs IC for ILA, OH-PLA and lactic acid were of 1.47, 1.80, and 3.92mg/mL, respectively. The antiaflatoxigenic properties of LAB depend on strain's capability to produce lactic acid, PLA, OH-PLA and ILA.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Aflatoxins); 0 (Antifungal Agents); 0 (Biological Control Agents); 33X04XA5AT (Lactic Acid)


  2 / 816 MEDLINE  
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PMID:28745426
Autor:Tomada S; Sonego P; Moretto M; Engelen K; Pertot I; Perazzolli M; Puopolo G
Endereço:Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all'Adige, Italy.
Título:Dual RNA-Seq of Lysobacter capsici AZ78 - Phytophthora infestans interaction shows the implementation of attack strategies by the bacterium and unsuccessful oomycete defense responses.
Fonte:Environ Microbiol; 19(10):4113-4125, 2017 Oct.
ISSN:1462-2920
País de publicação:England
Idioma:eng
Resumo:Biological interactions in the microbial communities of the rhizosphere continuously shape the gene expression patterns of each individual microorganism. A dual RNA-Seq approach was applied to obtain a comprehensive overview of the molecular mechanisms activated during the interaction between the biocontrol rhizobacterium Lysobacter capsici AZ78 and the soilborne phytopathogenic oomycete Phytophthora infestans. The RNA-Seq transcriptional profile of L. capsici AZ78 was characterized by up-regulation of genes concerned in the biogenesis of type 4 pilus and lytic enzymes, involved, respectively, in host colonization and subsequent attack of the P. infestans cell wall. The activation of detoxification processes allowed L. capsici AZ78 to overcome the attempted defense processes of P. infestans. Moreover, the genes involved in antibiotic biosynthesis were up-regulated in L. capsici AZ78 and caused cell death in P. infestans, with the activation of putative apoptotic processes. The consequences of P. infestans cell death resulted in the down-regulation of primary metabolic pathways, such as carbohydrates, nucleic acids and protein metabolisms. Overall, the mechanism of action of L. capsici AZ78 was related to parasitism and predatory activities that cause the death of P. infestans.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biological Control Agents); 0 (RNA, Bacterial); 0 (RNA, Protozoan)


  3 / 816 MEDLINE  
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PMID:29338057
Autor:Ye BH; Zhang YB; Shu JP; Wu H; Wang HJ
Endereço:Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China.
Título:RNA-sequencing analysis of fungi-induced transcripts from the bamboo wireworm Melanotus cribricollis (Coleoptera: Elateridae) larvae.
Fonte:PLoS One; 13(1):e0191187, 2018.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Larvae of Melanotus cribricollis, feed on bamboo shoots and roots, causing serious damage to bamboo in Southern China. However, there is currently no effective control measure to limit the population of this underground pest. Previously, a new entomopathogenic fungal strain isolated from M. cribricollis larvae cadavers named Metarhizium pingshaense WP08 showed high pathogenic efficacy indoors, indicated that the fungus could be used as a bio-control measure. So far, the genetic backgrounds of both M. cribricollis and M. pingshaense WP08 were blank. Here, we analyzed the whole transcriptome of M. cribricollis larvae, infected with M. pingshaense WP08 or not, using high-throughput next generation sequencing technology. In addition, the transcriptome sequencing of M. pingshaense WP08 was also performed for data separation of those two non-model species. The reliability of the RNA-Seq data was also validated through qRT-PCR experiment. The de novo assembly, functional annotation, sequence comparison of four insect species, and analysis of DEGs, enriched pathways, GO terms and immune related candidate genes were operated. The results indicated that, multiple defense mechanisms of M. cribricollis larvae are initiated to protect against the more serious negative effects caused by fungal infection. To our knowledge, this was the first report of transcriptome analysis of Melanotus spp. infected with a fungus, and it could provide insights to further explore insect-fungi interaction mechanisms.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Biological Control Agents)


  4 / 816 MEDLINE  
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PMID:28461206
Autor:Chen L
Endereço:College of Bioengineering, Henan University of Technology, Zhengzhou 450001, PR China; Collaborative Innovation Center of Grain Storage Security in Henan Province, Zhengzhou 450001, PR China. Electronic address: chen_liang.cl@163.com.
Título:Complete genome sequence of Bacillus velezensis LM2303, a biocontrol strain isolated from the dung of wild yak inhabited Qinghai-Tibet plateau.
Fonte:J Biotechnol; 251:124-127, 2017 Jun 10.
ISSN:1873-4863
País de publicação:Netherlands
Idioma:eng
Resumo:Bacillus velezensis LM2303 is a biocontrol strain with a broad inhibitory spectrum against plant pathogens, isolated from the dung of wild yak inhabited Qinghai-Tibet plateau, China. Here we present its complete genome sequence, which consists of a single, circular chromosome of 3,989,393bp with a 46.68% G+C content. Genome analysis revealed genes encoding specialized functions for the biosynthesis of antifungal metabolites and antibacterial metabolites, the promotion of plant growth, the alleviation of oxidative stress and nutrient utilization. And the biosynthesis of antimicrobial metabolites in strain LM2303 was confirmed by biochemical analysis, while its plant growth promoting traits were confirmed by inoculation tests. Our results will establish a better foundation for further studies and biocontrol application of B. velezensis LM2303.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biological Control Agents)


  5 / 816 MEDLINE  
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PMID:29281634
Autor:Bassène MD; Seck MT; Pagabeleguem S; Fall AG; Sall B; Vreysen MJB; Gimonneau G; Bouyer J
Endereço:Institut Sénégalais de Recherches Agricoles, Laboratoire National d'Elevage et de Recherches Vétérinaires, Dakar-Hann, Sénégal.
Título:Competitiveness and survival of two strains of Glossina palpalis gambiensis in an urban area of Senegal.
Fonte:PLoS Negl Trop Dis; 11(12):e0006172, 2017 12.
ISSN:1935-2735
País de publicação:United States
Idioma:eng
Resumo:BACKGROUND: In the Niayes area, located in the west of Senegal, only one tsetse species, Glossina palpalis gambiensis Vanderplank (Diptera: Glossinidae) was present. The Government of Senegal initiated and implemented an elimination programme in this area that included a sterile insect technique (SIT) component. The G. p. gambiensis strain (BKF) mass-reared at the Centre International de Recherche-Développement sur l'Elevage en zone Subhumide (CIRDES) in Burkina Faso was used for the SIT component. METHODOLOGY/PRINCIPAL FINDINGS: Studies conducted in 2011 in four localities in the Niayes area (Pout, Sébikotane, Diacksao Peul and the Parc de Hann) showed that the BKF strain demonstrated inferior survival in the ecosystem of the Parc de Hann, a forested area in the city centre of the capital Dakar. Therefore, G. p. gambiensis flies from the Niayes area (SEN strain) were colonized. Here we compared the competitiveness and survival of the two strains (BKF and SEN) in the Parc de Hann. Released sterile males of the SEN colony showed a daily mortality rate of 0.08 (SD 0.08) as compared with 0.14 (SD 0.08) for the BKF flies but the difference was not significant (p-value = 0.14). However, the competitiveness of the SEN males was lower (0.14 (SD 0.10)) as compared with that of the BKF males (0.76 (SD 0.11)) (p-value < 10-3). CONCLUSIONS/SIGNIFICANCE: Based on the results of this study, it can be concluded that the BKF strain will remain the main strain to be used in the elimination programme. Despite the slightly longer survival of the SEN males in the Parc de Hann, the superior competitiveness of the BKF males is deemed more important for the SIT component, as their shorter survival rates can be easily compensated for by more frequent fly releases.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Biological Control Agents)


  6 / 816 MEDLINE  
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PMID:28813440
Autor:Strydom M; Veldtman R; Ngwenya MZ; Esler KJ
Endereço:Department of Conservation Ecology and Entomology, Stellenbosch University, Matieland, South Africa.
Título:Invasive Australian Acacia seed banks: Size and relationship with stem diameter in the presence of gall-forming biological control agents.
Fonte:PLoS One; 12(8):e0181763, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Australian Acacia are invasive in many parts of the world. Despite significant mechanical and biological efforts to control their invasion and spread, soil-stored seed banks prevent their effective and sustained removal. In response South Africa has had a strong focus on employing seed reducing biological control agents to deal with Australian Acacia invasion, a programme that is considered as being successful. To provide a predictive understanding for their management, seed banks of four invasive Australian acacia species (Acacia longifolia, A. mearnsii, A. pycnantha and A. saligna) were studied in the Western Cape of South Africa. Across six to seven sites for each species, seed bank sizes were estimated from dense, monospecific stands by collecting 30 litter and soil samples. Average estimated seed bank size was large (1017 to 17261 seed m-2) as was annual input into the seed bank, suggesting that these seed banks are not residual but are replenished in size annually. A clear relationship between seed bank size and stem diameter was established indicating that mechanical clearing should be conducted shortly after fire-stimulated recruitment events or within old populations when seed banks are small. In dense, monospecific stands seed-feeding biological control agents are not effective in reducing seed bank size.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biological Control Agents); 0 (Soil)


  7 / 816 MEDLINE  
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PMID:28800614
Autor:Vatanparast M; Kim Y
Endereço:Department of Plant Protection, College of Agriculture, University of Bu-Ali Sina, Hamedan, Iran.
Título:Optimization of recombinant bacteria expressing dsRNA to enhance insecticidal activity against a lepidopteran insect, Spodoptera exigua.
Fonte:PLoS One; 12(8):e0183054, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Double-stranded RNA (dsRNA) has been applied to control insect pests due to its induction of RNA interference (RNAi) of a specific target gene expression. However, developing dsRNA-based insecticidal agent has been a great challenge especially against lepidopteran insect pests due to variations in RNAi efficiency. The objective of this study was to screen genes of chymotrypsins (SeCHYs) essential for the survival of the beet armyworm, Spodoptera exigua, to construct insecticidal dsRNA. In addition, an optimal oral delivery method was developed using recombinant bacteria. At least 7 SeCHY genes were predicted from S. exigua transcriptomes. Subsequent analyses indicated that SeCHY2 was widely expressed in different developmental stages and larval tissues by RT-PCR and its expression knockdown by RNAi caused high mortality along with immunosuppression. However, a large amount of dsRNA was required to efficiently kill late instars of S. exigua because of high RNase activity in their midgut lumen. To minimize dsRNA degradation, bacterial expression and formulation of dsRNA were performed in HT115 Escherichia coli using L4440 expression vector. dsRNA (300 bp) specific to SeCHY2 overexpressed in E. coli was toxic to S. exigua larvae after oral administration. To enhance dsRNA release from E. coli, bacterial cells were sonicated before oral administration. RNAi efficiency of sonicated bacteria was significantly increased, causing higher larval mortality at oral administration. Moreover, targeting young larvae possessing weak RNase activity in the midgut lumen significantly enhanced RNAi efficiency and subsequent insecticidal activity against S. exigua.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Biological Control Agents); 0 (Insect Proteins); 0 (Protein Isoforms); 0 (RNA, Double-Stranded); EC 3.4.21.1 (Chymotrypsin)


  8 / 816 MEDLINE  
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PMID:28792417
Autor:Gulla S; Rønneseth A; Sørum H; Vågnes Ø; Balboa S; Romalde JL; Colquhoun DJ
Endereço:Norwegian Veterinary Institute, Pb 750 Sentrum, 0106 Oslo, Norway.
Título:Vibrio tapetis from wrasse used for ectoparasite bio-control in salmon farming: phylogenetic analysis and serotyping.
Fonte:Dis Aquat Organ; 125(3):189-197, 2017 08 09.
ISSN:0177-5103
País de publicação:Germany
Idioma:eng
Resumo:So-called 'cleaner fish', including various wrasse (Labridae) species, have become increasingly popular in Norwegian salmon farming in recent years for biocontrol of the salmon louse Lepeophtheirus salmonis. Cleaner fish mortalities in salmon farms are, however, often high. Various bacterial agents are frequently associated with episodes of increased cleaner fish mortality, and Vibrio tapetis is regularly cultured from diseased wrasse. In the present study, we investigated the genetic relationships among 54 V. tapetis isolates (34 from wrasse species) by multilocus sequence analysis (MLSA; rpoD, ftsZ, pyrH, rpoA and atpA). In the resulting phylogenetic tree, all wrasse isolates belonged to sub-clusters within V. tapetis subsp. tapetis. Slide agglutination testing further confirmed the complete dominance amongst these isolates of 4 O-antigen serotypes, designated here as V. tapetis subsp. tapetis serotypes O1, O3, O4 and O5, respectively. A pilot challenge trial using serotypes O3, O4 and O5 did not indicate high pathogenicity towards ballan wrasse Labrus bergylta, thus questioning the role of V. tapetis as a primary pathogen of this fish species.
Tipo de publicação: CLINICAL TRIAL; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (Biological Control Agents)


  9 / 816 MEDLINE  
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PMID:28683101
Autor:Han B; Zhang S; Zeng F; Mao J
Endereço:Key Laboratory for Biology of Plant Diseases and Insect Pests, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Título:Nutritional and reproductive signaling revealed by comparative gene expression analysis in Chrysopa pallens (Rambur) at different nutritional statuses.
Fonte:PLoS One; 12(7):e0180373, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:BACKGROUND: The green lacewing, Chrysopa pallens Rambur, is one of the most important natural predators because of its extensive spectrum of prey and wide distribution. However, what we know about the nutritional and reproductive physiology of this species is very scarce. RESULTS: By cDNA amplification and Illumina short-read sequencing, we analyzed transcriptomes of C. pallens female adult under starved and fed conditions. In total, 71236 unigenes were obtained with an average length of 833 bp. Four vitellogenins, three insulin-like peptides and two insulin receptors were annotated. Comparison of gene expression profiles suggested that totally 1501 genes were differentially expressed between the two nutritional statuses. KEGG orthology classification showed that these differentially expression genes (DEGs) were mapped to 241 pathways. In turn, the top 4 are ribosome, protein processing in endoplasmic reticulum, biosynthesis of amino acids and carbon metabolism, indicating a distinct difference in nutritional and reproductive signaling between the two feeding conditions. CONCLUSIONS: Our study yielded large-scale molecular information relevant to C. pallens nutritional and reproductive signaling, which will contribute to mass rearing and commercial use of this predaceous insect species.
Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE
Nome de substância:0 (Biological Control Agents); 0 (DNA, Complementary); 0 (Insect Proteins); 0 (Intercellular Signaling Peptides and Proteins); 0 (Vitellogenins); EC 2.7.10.1 (Receptor, Insulin)


  10 / 816 MEDLINE  
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PMID:28644879
Autor:Wu Q; Sun R; Ni M; Yu J; Li Y; Yu C; Dou K; Ren J; Chen J
Endereço:Department of Environment and Resource, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Título:Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy.
Fonte:PLoS One; 12(6):e0179957, 2017.
ISSN:1932-6203
País de publicação:United States
Idioma:eng
Resumo:Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of Trichoderma asperellum, named GDFS1009. The mycelium of T. asperellum GDFS1009 exhibits a high growth rate, high sporulation capacity, and strong inhibitory effects against pathogens that cause cucumber fusarium wilt and corn stalk rot. T. asperellum GDFS1009 secretes chitinase, glucanase, and protease, which can degrade the cell walls of fungi and contribute to mycoparasitism. The secreted xylanases are good candidates for inducing plant resistance and enhancing plant immunity against pathogens. RNA sequencing (RNA-seq) and gas chromatography-mass spectrometry (GC-MS) showed that T. asperellum GDFS1009 produces primary metabolites that are precursors of antimicrobial compounds; it also produces a variety of antimicrobial secondary metabolites, including polyketides and alkanes. In addition, this study speculated the presence of six antimicrobial peptides via ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS). Future studies should focus on these antimicrobial metabolites for facilitating widespread application in the field of agricultural bio-control.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:0 (Alkanes); 0 (Anti-Infective Agents); 0 (Biological Control Agents); 0 (Fungal Proteins); 0 (Polyketides)



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