Base de dados : MEDLINE
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  1 / 71 MEDLINE  
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[PMID]:26204184
[Au] Autor:Lukásová K; Holusa J
[Ti] Título:Comparison of pathogens infection level in Ips typographus (Coleoptera: Curculionidae) beetles sampled in pheromone traps and at place of overwintering.
[So] Source:Acta Parasitol;60(3):462-5, 2015 Sep.
[Is] ISSN:1896-1851
[Cp] País de publicação:Poland
[La] Idioma:ENG
[Ab] Resumo:The importance of pathogens in the population dynamics of Ips typographus remains a subject of ongoing debate. The main objective of our experiment was to compare the pathogen infection levels of individuals overwintering in bark with the levels of individuals from the same population captured with pheromone traps and thereby to determine primary answers as to whether it can be confirmed that pathogenic organisms affect the flight ability of bark beetles or their ability to leave their places of overwintering. A total of 402 I. typographus individuals were analyzed at a study location under limited management. Three pathogens were confirmed to be present: the gregarine Gregarina typographi, the virus ItEPV, and the microsporidium Nosema typographi. Infection levels of Gregarina typographi and ItEPV were the same in beetles collected at places of overwintering and in those beetles collected in pheromone traps within the immediate vicinity. As these pathogens infect the host's intestine, the tendency to leave the places of overwintering is apparently not diminished. A similar analysis and comparison of pathogens located in the fat body might bring different results, as our study only detected N. typographi in a single dissected adult spruce bark beetle.
[Mh] Termos MeSH primário: Apicomplexa/isolamento & purificação
Entomopoxvirinae/isolamento & purificação
Nosema/isolamento & purificação
Gorgulhos/microbiologia
Gorgulhos/parasitologia
[Mh] Termos MeSH secundário: Animais
Gorgulhos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:015724
[St] Status:MEDLINE


  2 / 71 MEDLINE  
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[PMID]:26499185
[Au] Autor:Nakai M; Kinjo H; Takatsuka J; Shiotsuki T; Kamita SG; Kunimi Y
[Ad] Endereço:1​ Tokyo University of Agriculture and Technology, Saiwai, , Fuchu, Tokyo 183-8509, Japan....
[Ti] Título:Entomopoxvirus infection induces changes in both juvenile hormone and ecdysteroid levels in larval Mythimna separata.
[So] Source:J Gen Virol;97(1):225-32, 2016 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Insect viruses are among the most important pathogens of lepidopteran insects. Virus-infected larvae often show developmental defects including a prolonged larval period and a failure to pupate, but the mechanisms by which insect viruses regulate host development need further investigation. In this study, the regulation of host endocrinology by a lepidopteran entomopoxvirus (EPV), Mythimna separata EPV (MySEV), was examined. When fourth instar M. separata were inoculated with MySEV occlusion bodies, pupation was prevented and the insects died during the final (sixth) larval instar. Liquid chromatography-MS analysis revealed that juvenile hormone (JH) titres in the haemolymph of MySEV-infected sixth instars were higher than those in mock-infected larvae. JH esterase (JHE) activity was also examined by kinetic assay using a colorimetric substrate. The level of JHE activity in the haemolymph of MySEV-infected larvae was generally lower than that found in mock-infected larvae. In contrast, ecdysteroid titre in the haemolymph of final-instar MySEV-infected larvae was lower than that found in mock-infected larvae when measured by radioimmunoassay. A statistically significant difference in the release of ecdysteroids from prothoracic glands (PGs) that were dissected from MySEV- or mock-infected sixth instar Day 3 larvae was not found following prothoracicotropic hormone (PTTH) exposure. Our results indicate that the release of ecdysteroids was reduced not by infection of the PGs by MySEV, but by reduced PTTH production from the brain. Taken together our study suggests that EPVs retard host development by both reducing ecdysone titre and maintaining status quo levels of JH by preventing its metabolism.
[Mh] Termos MeSH primário: Ecdisteroides/análise
Entomopoxvirinae/fisiologia
Interações Hospedeiro-Patógeno
Hormônios Juvenis/análise
Lepidópteros/virologia
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida
Esterases/análise
Hemolinfa/química
Larva/crescimento & desenvolvimento
Larva/virologia
Lepidópteros/crescimento & desenvolvimento
Espectrometria de Massas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ecdysteroids); 0 (Juvenile Hormones); EC 3.1.- (Esterases)
[Em] Mês de entrada:1605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000325


  3 / 71 MEDLINE  
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[PMID]:25871928
[Au] Autor:Thézé J; Takatsuka J; Nakai M; Arif B; Herniou EA
[Ad] Endereço:Institut de Recherche sur la Biologie de l'Insecte, CNRS UMR 7261, Université François-Rabelais, UFR Sciences et Techniques, 37200 Tours, France. theze.julien@gmail.com....
[Ti] Título:Gene acquisition convergence between entomopoxviruses and baculoviruses.
[So] Source:Viruses;7(4):1960-74, 2015 Apr.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Organisms from diverse phylogenetic origins can thrive within the same ecological niches. They might be induced to evolve convergent adaptations in response to a similar landscape of selective pressures. Their genomes should bear the signature of this process. The study of unrelated virus lineages infecting the same host panels guarantees a clear identification of phyletically independent convergent adaptation. Here, we investigate the evolutionary history of genes in the accessory genome shared by unrelated insect large dsDNA viruses: the entomopoxviruses (EPVs, Poxviridae) and the baculoviruses (BVs). EPVs and BVs have overlapping ecological niches and have independently evolved similar infection processes. They are, in theory, subjected to the same selective pressures from their host's immune responses. Their accessory genomes might, therefore, bear analogous genomic signatures of convergent adaption and could point out key genomic mechanisms of adaptation hitherto undetected in viruses. We uncovered 32 homologous, yet independent acquisitions of genes originating from insect hosts, different eukaryotes, bacteria and viruses. We showed different evolutionary levels of gene acquisition convergence in these viruses, underlining a continuous evolutionary process. We found both recent and ancient gene acquisitions possibly involved to the adaptation to both specific and distantly related hosts. Multidirectional and multipartite gene exchange networks appear to constantly drive exogenous gene assimilations, bringing key adaptive innovations and shaping the life histories of large DNA viruses. This evolutionary process might lead to genome level adaptive convergence.
[Mh] Termos MeSH primário: Baculoviridae/genética
Entomopoxvirinae/genética
Evolução Molecular
Transferência Genética Horizontal
Insetos/virologia
[Mh] Termos MeSH secundário: Adaptação Biológica
Animais
Baculoviridae/fisiologia
DNA Viral/química
DNA Viral/genética
Entomopoxvirinae/fisiologia
Dados de Sequência Molecular
Seleção Genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150507
[Lr] Data última revisão:
150507
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150415
[St] Status:MEDLINE
[do] DOI:10.3390/v7041960


  4 / 71 MEDLINE  
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[PMID]:25591507
[Au] Autor:Özsahin E; Sezen K; Demirbag Z
[Ad] Endereço:Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
[Ti] Título:Amsacta moorei entomopoxvirus encodes a functional esterase (amv133) with protease activity.
[So] Source:Intervirology;58(1):41-8, 2015.
[Is] ISSN:1423-0100
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Lipolytic genes have been investigated in several viral genomes, and some of them show enzyme activity which can be used for various functions including the production of DNA replication metabolites, rescue from endosomes, and membrane fusion. Amsacta moorei entomopoxvirus (AMEV) replicates in nearly the entire insect body, especially in the adipose tissue. One of the open reading frames (ORFs) in the AMEV genome, amv133, encodes a putative lipase enzyme. In this study, we therefore investigate the enzyme activity of amv133. METHODS: amv133 was aligned with known lipase genes and their homologs in entomopoxviruses. Expressed proteins were partially purified and assayed for lipase, esterase and protease. RESULTS: We found that amv133 contains all the domains required for a functional lipase enzyme and that it shows a significant similarity with homologs in other entomopoxviruses. Since there is a similarity of the catalytic triad between lipases and serine proteases, we also investigated the protease activity of amv133. Lipase, esterase and protease assays showed that amv133 encodes a functional esterase enzyme with protease activity. CONCLUSION: The current data show that amv133 is a conserved gene in all entomopoxvirus genomes sequenced so far and might contribute greatly to degrading the lipids or proteins and hence improve the virus infection.
[Mh] Termos MeSH primário: Entomopoxvirinae/enzimologia
Esterases/genética
Esterases/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Entomopoxvirinae/genética
Entomopoxvirinae/metabolismo
Esterases/química
Genes Virais
Genoma Viral
Insetos/virologia
Lipase/genética
Dados de Sequência Molecular
Fases de Leitura Aberta
Peptídeo Hidrolases/metabolismo
Alinhamento de Sequência
Proteínas Virais/química
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Proteins); EC 3.1.- (Esterases); EC 3.1.1.3 (Lipase); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150303
[St] Status:MEDLINE
[do] DOI:10.1159/000369018


  5 / 71 MEDLINE  
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[PMID]:24796553
[Au] Autor:Ozsahin E; Sezen K; Demirbag Z
[Ad] Endereço:Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
[Ti] Título:Transcriptional analysis of ORF amv133 of Amsacta moorei entomopoxvirus.
[So] Source:Arch Virol;159(10):2541-7, 2014 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The open reading frame (ORF) amv133 of Amsacta moorei entomopoxvirus, encodes a putative lipase gene. Its temporal expression pattern was characterized by RT-PCR and found to start at 6 h postinfection (h p.i.) and reach a maximum level at 48 h p.i. While the ORF has a late promoter motif, the inhibition of viral DNA synthesis by Ara-C failed to inhibit transcription, but a general inhibitor of protein synthesis prevented its transcription, indicating that amv133 is an intermediate gene. 5'-RACE analysis showed that transcription was initiated at position -77 relative to the translational start site. To determine the size of the promoter, several truncations were generated and cloned upstream of the firefly luciferase reporter gene. The resulting constructs were tested in a dual assay. A fragment that contained 115 bp relative to the transcription start site exhibited optimum promoter length.
[Mh] Termos MeSH primário: Entomopoxvirinae/genética
Lipase/genética
Fases de Leitura Aberta/genética
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Animais
Antivirais/farmacologia
Sequência de Bases
Linhagem Celular
Citarabina/farmacologia
Replicação do DNA/efeitos de drogas
DNA Viral/genética
Regulação Viral da Expressão Gênica
Genes Virais
Dados de Sequência Molecular
Mariposas/virologia
Infecções por Poxviridae
Regiões Promotoras Genéticas
Biossíntese de Proteínas/efeitos de drogas
Sítio de Iniciação de Transcrição
Transcrição Genética/efeitos de drogas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Antiviral Agents); 0 (DNA, Viral); 0 (Viral Proteins); 04079A1RDZ (Cytarabine); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140924
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-014-2096-1


  6 / 71 MEDLINE  
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[PMID]:24603041
[Au] Autor:Takatsuka J; Nakai M
[Ad] Endereço:Forestry and Forest Products Research Institute, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan. Electronic address: junsan@ffpri.affrc.go.jp.
[Ti] Título:Replication of Mythimna separata entomopoxvirus in High Five™ cells and the construction of a recombinant.
[So] Source:J Invertebr Pathol;118:12-7, 2014 May.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mythimna separata entomopoxvirus (MySEV), of the genus Betaentomopoxvirus, was found to replicate in High Five™ cells. The infected cells produced many occlusion bodies and were hypertrophied but did not lyse. Following infection at a multiplicity of infection of 0.1, titers of extracellular virus reached a plateau 3-4days post infection at 25°C and were estimated at ca. 3×10(5) plaque-forming units per ml in TC-100 or TMN-FH media, both of which contained fetal bovine serum (FBS). Serum free medium, Express Five® SFM, also supported virus replication in High Five™ cells, but the titers were approximately one-tenth of those grown in TC-100 or TMN-FH media containing FBS. Using High Five™ cells, a recombinant MySEV was successfully constructed using homologous recombination. This study opens an avenue to the evaluation of entomopoxvirus gene functions using reverse genetic approaches with in vitro and in vivo hosts.
[Mh] Termos MeSH primário: Entomopoxvirinae/genética
Técnicas de Introdução de Genes/métodos
Genes Virais/genética
Lepidópteros/virologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Microscopia Eletrônica de Transmissão
Reação em Cadeia da Polimerase
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140422
[St] Status:MEDLINE


  7 / 71 MEDLINE  
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[PMID]:24606687
[Au] Autor:Mitsuhashi W; Miyamoto K; Wada S
[Ad] Endereço:National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: mitsuhas@affrc.go.jp.
[Ti] Título:The complete genome sequence of the Alphaentomopoxvirus Anomala cuprea entomopoxvirus, including its terminal hairpin loop sequences, suggests a potentially unique mode of apoptosis inhibition and mode of DNA replication.
[So] Source:Virology;452-453:95-116, 2014 Mar.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Complete genome sequence of Anomala cuprea entomopoxvirus, which belongs to the genus Alphaentomopoxvirus, including its terminal hairpin loop sequences, is reported. This is the first genome sequence of Alphaentomopoxvirus reported, and hairpin loops in entomopoxviruses have not previously been sequenced. The genome is 245,717 bp, which is smaller than had previously been estimated for Alphaentomopoxvirus. The inverted terminal repeats are quite long, and experimental results suggest that one genome molecule has one type of hairpin at one end and another type at the other end. The genome contains unexpected ORFs, e.g., that for the ubiquitin-conjugating enzyme E2 of eukaryotes. The BIR and RING domains found in a single ORF for an inhibitor of apoptosis in baculoviruses and entomopoxviruses occurred in two different, widely separated ORFs. Furthermore, an ORF in the genome contains a serpin domain that was previously found in vertebrate poxviruses for apoptosis inhibition but not in insect viruses.
[Mh] Termos MeSH primário: Apoptose
Replicação do DNA
Entomopoxvirinae/genética
Genoma Viral
Insetos/citologia
Sequências Repetidas Invertidas
Infecções por Poxviridae/veterinária
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA Viral/química
DNA Viral/genética
DNA Viral/metabolismo
Entomopoxvirinae/química
Entomopoxvirinae/fisiologia
Insetos/virologia
Dados de Sequência Molecular
Infecções por Poxviridae/fisiopatologia
Infecções por Poxviridae/virologia
Proteínas Virais/química
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1405
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140310
[St] Status:MEDLINE


  8 / 71 MEDLINE  
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[PMID]:23424042
[Au] Autor:Mitsuhashi W; Asano S; Miyamoto K; Wada S
[Ad] Endereço:National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
[Ti] Título:Further research on the biological function of inclusion bodies of Anomala cuprea entomopoxvirus, with special reference to the effect on the insecticidal activity of a Bacillus thuringiensis formulation.
[So] Source:Pest Manag Sci;70(1):46-54, 2014 Jan.
[Is] ISSN:1526-4998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Entomopoxviruses (EVs) form two types of inclusion body: spheroids, which contain virions, and spindles, which do not. The authors tested whether the spindles from a coleopteran EV, Anomala cuprea EV (ACEV), enhanced the insecticidal activity of a commercial Bacillus thuringiensis (Bt) formulation and the susceptibility of scarabaeid pest species in Japan to the virus's spheroids, to assess whether ACEV inclusion bodies are potential biological control agents for pest insects. RESULTS: Peroral inoculation with both ACEV spindles and the Bt toxin only or the complete Bt formulation shortened the survival and increased the mortality of treated insects compared with those of insects inoculated with Bt without the spindles (8-38 h of decrease in LT50 values among assays). ACEV showed high infectivity to a major scarabaeid pest species in Japanese sugar cane fields. CONCLUSION: The results suggest that spindles or the constituent protein fusolin can be used as a coagent with Bt formulations, and that fusolin coexpression with a Bt toxin in crops might improve the insecticidal efficacy. In addition, the spheroids are potential biocontrol agents for some scarabaeid pests that are not easy to control because of their underground habitation.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/farmacologia
Besouros/efeitos de drogas
Química Farmacêutica/métodos
Endotoxinas/química
Endotoxinas/farmacologia
Entomopoxvirinae/química
Proteínas Hemolisinas/química
Proteínas Hemolisinas/farmacologia
Corpos de Inclusão/química
Controle Biológico de Vetores/métodos
[Mh] Termos MeSH secundário: Animais
Entomopoxvirinae/metabolismo
Corpos de Inclusão/metabolismo
Controle Biológico de Vetores/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (insecticidal crystal protein, Bacillus Thuringiensis)
[Em] Mês de entrada:1410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140304
[St] Status:MEDLINE
[do] DOI:10.1002/ps.3521


  9 / 71 MEDLINE  
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[PMID]:23620379
[Au] Autor:Perera S; Krell P; Demirbag Z; Nalçacioglu R; Arif B
[Ad] Endereço:Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste. Marie, Ontario, Canada.
[Ti] Título:Induction of apoptosis by the Amsacta moorei entomopoxvirus.
[So] Source:J Gen Virol;94(Pt 8):1876-87, 2013 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CF-70-B2 cells derived from the spruce budworm (Choristoneura fumiferana) undergo apoptosis when infected with Amsacta moorei entomopoxvirus (AMEV), as characterized by membrane blebbing, formation of apoptotic bodies, TdT-mediated dUTP nick-end labelling (TUNEL) staining, condensed chromatin and induction of caspase-3/7 activity. The apoptotic response was reduced when cells were infected with UV-inactivated AMEV, but not when infected in the presence of the DNA synthesis inhibitor, cytosine ß-d-arabinofuranoside. Hence, only pre-DNA replication events were involved in inducing the antiviral response in CF-70-B2 cells. The virus eventually overcame the host's antiviral response and replicated to high progeny virus titres accompanied by high levels of caspase-3/7 activity. The CF-70-B2 cells were less productive of progeny virus in comparison to LD-652, a Lymantria dispar cell line routinely used for propagation of AMEV. At late stages of infection, LD-652 cells also showed characteristics of apoptosis such as oligosomal DNA fragmentation, TUNEL staining, condensed chromatin and increased caspase-3/7 activity. Induction of apoptosis in LD-652 cells was dependent on viral DNA replication and/or late gene expression. A significantly reduced rate of infection was observed in the presence of general caspase inhibitors Q-VD-OPH and Z-VAD-FMK, indicating caspases may be involved in productive virus infection.
[Mh] Termos MeSH primário: Apoptose
Entomopoxvirinae/patogenicidade
Lepidópteros/virologia
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Caspase 7/metabolismo
Linhagem Celular
Membrana Celular/patologia
Fragmentação do DNA
Marcação In Situ das Extremidades Cortadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 7)
[Em] Mês de entrada:1309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130716
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.051888-0


  10 / 71 MEDLINE  
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[PMID]:23678178
[Au] Autor:Thézé J; Takatsuka J; Li Z; Gallais J; Doucet D; Arif B; Nakai M; Herniou EA
[Ad] Endereço:Institut de Recherche sur la Biologie de l'Insecte, Unité Mixte de Recherche (UMR) 7261, Centre National de la Recherche Scientifique (CNRS), Université François-Rabelais, UFR Sciences et Techniques, Tours, France. theze.julien@gmail.com
[Ti] Título:New insights into the evolution of Entomopoxvirinae from the complete genome sequences of four entomopoxviruses infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata.
[So] Source:J Virol;87(14):7992-8003, 2013 Jul.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Poxviruses are nucleocytoplasmic large DNA viruses encompassing two subfamilies, the Chordopoxvirinae and the Entomopoxvirinae, infecting vertebrates and insects, respectively. While chordopoxvirus genomics have been widely studied, only two entomopoxvirus (EPV) genomes have been entirely sequenced. We report the genome sequences of four EPVs of the Betaentomopoxvirus genus infecting the Lepidoptera: Adoxophyes honmai EPV (AHEV), Choristoneura biennis EPV (CBEV), Choristoneura rosaceana EPV (CREV), and Mythimna separata EPV (MySEV). The genomes are 80% AT rich, are 228 to 307 kbp long, and contain 247 to 334 open reading frames (ORFs). Most genes are homologous to those of Amsacta moorei entomopoxvirus and encode several protein families repeated in tandem in terminal regions. Some genomes also encode proteins of unknown functions with similarity to those of other insect viruses. Comparative genomic analyses highlight a high colinearity among the lepidopteran EPV genomes and little gene order conservation with other poxvirus genomes. As with previously sequenced EPVs, the genomes include a relatively conserved central region flanked by inverted terminal repeats. Protein clustering identified 104 core EPV genes. Among betaentomopoxviruses, 148 core genes were found in relatively high synteny, pointing to low genomic diversity. Whole-genome and spheroidin gene phylogenetic analyses showed that the lepidopteran EPVs group closely in a monophyletic lineage, corroborating their affiliation with the Betaentomopoxvirus genus as well as a clear division of the EPVs according to the orders of insect hosts (Lepidoptera, Coleoptera, and Orthoptera). This suggests an ancient coevolution of EPVs with their insect hosts and the need to revise the current EPV taxonomy to separate orthopteran EPVs from the lepidopteran-specific betaentomopoxviruses so as to form a new genus.
[Mh] Termos MeSH primário: Entomopoxvirinae/genética
Evolução Molecular
Genoma Viral/genética
Mariposas/virologia
Filogenia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Composição de Bases/genética
Sequência de Bases
Canadá
China
Entomopoxvirinae/classificação
Genômica
Japão
Funções Verossimilhança
Modelos Genéticos
Dados de Sequência Molecular
Fases de Leitura Aberta/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1309
[Cu] Atualização por classe:150426
[Lr] Data última revisão:
150426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130701
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.00453-13



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