Base de dados : MEDLINE
Pesquisa : B04.280.650.250 [Categoria DeCS]
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[PMID]:24606687
[Au] Autor:Mitsuhashi W; Miyamoto K; Wada S
[Ad] Endereço:National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: mitsuhas@affrc.go.jp.
[Ti] Título:The complete genome sequence of the Alphaentomopoxvirus Anomala cuprea entomopoxvirus, including its terminal hairpin loop sequences, suggests a potentially unique mode of apoptosis inhibition and mode of DNA replication.
[So] Source:Virology;452-453:95-116, 2014 Mar.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Complete genome sequence of Anomala cuprea entomopoxvirus, which belongs to the genus Alphaentomopoxvirus, including its terminal hairpin loop sequences, is reported. This is the first genome sequence of Alphaentomopoxvirus reported, and hairpin loops in entomopoxviruses have not previously been sequenced. The genome is 245,717 bp, which is smaller than had previously been estimated for Alphaentomopoxvirus. The inverted terminal repeats are quite long, and experimental results suggest that one genome molecule has one type of hairpin at one end and another type at the other end. The genome contains unexpected ORFs, e.g., that for the ubiquitin-conjugating enzyme E2 of eukaryotes. The BIR and RING domains found in a single ORF for an inhibitor of apoptosis in baculoviruses and entomopoxviruses occurred in two different, widely separated ORFs. Furthermore, an ORF in the genome contains a serpin domain that was previously found in vertebrate poxviruses for apoptosis inhibition but not in insect viruses.
[Mh] Termos MeSH primário: Apoptose
Replicação do DNA
Entomopoxvirinae/genética
Genoma Viral
Insetos/citologia
Sequências Repetidas Invertidas
Infecções por Poxviridae/veterinária
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA Viral/química
DNA Viral/genética
DNA Viral/metabolismo
Entomopoxvirinae/química
Entomopoxvirinae/fisiologia
Insetos/virologia
Dados de Sequência Molecular
Infecções por Poxviridae/fisiopatologia
Infecções por Poxviridae/virologia
Proteínas Virais/química
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1405
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140310
[St] Status:MEDLINE


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[PMID]:23620379
[Au] Autor:Perera S; Krell P; Demirbag Z; Nalçacioglu R; Arif B
[Ad] Endereço:Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste. Marie, Ontario, Canada.
[Ti] Título:Induction of apoptosis by the Amsacta moorei entomopoxvirus.
[So] Source:J Gen Virol;94(Pt 8):1876-87, 2013 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CF-70-B2 cells derived from the spruce budworm (Choristoneura fumiferana) undergo apoptosis when infected with Amsacta moorei entomopoxvirus (AMEV), as characterized by membrane blebbing, formation of apoptotic bodies, TdT-mediated dUTP nick-end labelling (TUNEL) staining, condensed chromatin and induction of caspase-3/7 activity. The apoptotic response was reduced when cells were infected with UV-inactivated AMEV, but not when infected in the presence of the DNA synthesis inhibitor, cytosine ß-d-arabinofuranoside. Hence, only pre-DNA replication events were involved in inducing the antiviral response in CF-70-B2 cells. The virus eventually overcame the host's antiviral response and replicated to high progeny virus titres accompanied by high levels of caspase-3/7 activity. The CF-70-B2 cells were less productive of progeny virus in comparison to LD-652, a Lymantria dispar cell line routinely used for propagation of AMEV. At late stages of infection, LD-652 cells also showed characteristics of apoptosis such as oligosomal DNA fragmentation, TUNEL staining, condensed chromatin and increased caspase-3/7 activity. Induction of apoptosis in LD-652 cells was dependent on viral DNA replication and/or late gene expression. A significantly reduced rate of infection was observed in the presence of general caspase inhibitors Q-VD-OPH and Z-VAD-FMK, indicating caspases may be involved in productive virus infection.
[Mh] Termos MeSH primário: Apoptose
Entomopoxvirinae/patogenicidade
Lepidópteros/virologia
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Caspase 7/metabolismo
Linhagem Celular
Membrana Celular/patologia
Fragmentação do DNA
Marcação In Situ das Extremidades Cortadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 7)
[Em] Mês de entrada:1309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130716
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.051888-0


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[PMID]:23678178
[Au] Autor:Thézé J; Takatsuka J; Li Z; Gallais J; Doucet D; Arif B; Nakai M; Herniou EA
[Ad] Endereço:Institut de Recherche sur la Biologie de l'Insecte, Unité Mixte de Recherche (UMR) 7261, Centre National de la Recherche Scientifique (CNRS), Université François-Rabelais, UFR Sciences et Techniques, Tours, France. theze.julien@gmail.com
[Ti] Título:New insights into the evolution of Entomopoxvirinae from the complete genome sequences of four entomopoxviruses infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata.
[So] Source:J Virol;87(14):7992-8003, 2013 Jul.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Poxviruses are nucleocytoplasmic large DNA viruses encompassing two subfamilies, the Chordopoxvirinae and the Entomopoxvirinae, infecting vertebrates and insects, respectively. While chordopoxvirus genomics have been widely studied, only two entomopoxvirus (EPV) genomes have been entirely sequenced. We report the genome sequences of four EPVs of the Betaentomopoxvirus genus infecting the Lepidoptera: Adoxophyes honmai EPV (AHEV), Choristoneura biennis EPV (CBEV), Choristoneura rosaceana EPV (CREV), and Mythimna separata EPV (MySEV). The genomes are 80% AT rich, are 228 to 307 kbp long, and contain 247 to 334 open reading frames (ORFs). Most genes are homologous to those of Amsacta moorei entomopoxvirus and encode several protein families repeated in tandem in terminal regions. Some genomes also encode proteins of unknown functions with similarity to those of other insect viruses. Comparative genomic analyses highlight a high colinearity among the lepidopteran EPV genomes and little gene order conservation with other poxvirus genomes. As with previously sequenced EPVs, the genomes include a relatively conserved central region flanked by inverted terminal repeats. Protein clustering identified 104 core EPV genes. Among betaentomopoxviruses, 148 core genes were found in relatively high synteny, pointing to low genomic diversity. Whole-genome and spheroidin gene phylogenetic analyses showed that the lepidopteran EPVs group closely in a monophyletic lineage, corroborating their affiliation with the Betaentomopoxvirus genus as well as a clear division of the EPVs according to the orders of insect hosts (Lepidoptera, Coleoptera, and Orthoptera). This suggests an ancient coevolution of EPVs with their insect hosts and the need to revise the current EPV taxonomy to separate orthopteran EPVs from the lepidopteran-specific betaentomopoxviruses so as to form a new genus.
[Mh] Termos MeSH primário: Entomopoxvirinae/genética
Evolução Molecular
Genoma Viral/genética
Mariposas/virologia
Filogenia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Composição de Bases/genética
Sequência de Bases
Canadá
China
Entomopoxvirinae/classificação
Genômica
Japão
Funções Verossimilhança
Modelos Genéticos
Dados de Sequência Molecular
Fases de Leitura Aberta/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130701
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.00453-13


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[PMID]:22841637
[Au] Autor:Arif B; Pavlik L
[Ad] Endereço:Great Lakes Forestry Centre, Sault Ste. Marie, ON, Canada. barif@nrcan.gc.ca
[Ti] Título:Insect cell culture: virus replication and applications in biotechnology.
[So] Source:J Invertebr Pathol;112 Suppl:S138-41, 2013 Mar.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insect cell lines have been initiated since the 1930s and were used to replicate insect baculoviruses as well as arboviruses. Since the latter group of viruses cause serious diseased in man and equines, efforts were expended to characterize the viruses in the new cell lines in attempts to understand the replication cycle at the cellular and molecular levels. Soon it was realized that insect baculoviruses have a potential as viable alternatives to chemicals in the control of agricultural and forest insect pests. The cell lines provided excellent tools to understand the molecular biology of baculoviruses before wide-scale use in the field. During these investigastions, it came to light that baculoviruses can be exploited as vectors for the expression of exogenous proteins and vaccines. The amenability of the virus to genetic modifications and the increasing numbers of permissive cell lines opened new avenues in protein expression. However, not all baculoviruses were able to replicate in cell lines. Indeed, there are no cell lines permissive to viruses belonging to the genera Gammabaculvirus and Deltabaculovirus. Some entomopoxviruses have been replicated in a few cell lines and this paper reports the replication of an entomopoxvirus from the spruce budworm in a homologous cell line.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/métodos
Entomopoxvirinae/fisiologia
Mariposas/virologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Células Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130401
[St] Status:MEDLINE


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[PMID]:22899338
[Au] Autor:Salvador R; Ferrelli ML; Berretta MF; Mitsuhashi W; Biedma ME; Romanowski V; Sciocco-Cap A
[Ad] Endereço:Instituto de Microbiología y Zoología Agrícola, Castelar, Buenos Aires, Argentina. rsalvador@cnia.inta.gov.ar
[Ti] Título:Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus.
[So] Source:Virus Genes;45(3):610-3, 2012 Dec.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long (the smallest gp37 sequenced at present) and encodes a predicted 222-amino acid protein. This protein is glycosylated and specifically recognized by an entomopoxvirus fusolin antiserum. The pairwise comparison of EpapGV gp37 gene product with all the baculovirus sequences in GenBank yields high similarity values ranging from 45 to 63 % with Cydia pomonella Granulovirus gp37 being the most closely related. The phylogenetic analysis interestingly grouped the granuloviruses in a cluster more closely related to entomopoxviruses than to nucleopolyhedroviruses, suggesting a possible horizontal transfer event between the granulovirus group and the entomopoxvirus group.
[Mh] Termos MeSH primário: Entomopoxvirinae/genética
Genes Virais
Granulovirus/genética
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Entomopoxvirinae/classificação
Entomopoxvirinae/imunologia
Entomopoxvirinae/patogenicidade
Transferência Genética Horizontal
Glicosilação
Granulovirus/classificação
Granulovirus/imunologia
Granulovirus/patogenicidade
Soros Imunes/imunologia
Lepidópteros/virologia
Fases de Leitura Aberta
Filogenia
Homologia de Sequência de Aminoácidos
Proteínas do Envelope Viral/imunologia
Proteínas Virais/genética
Proteínas Virais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immune Sera); 0 (Viral Envelope Proteins); 0 (Viral Proteins); 0 (fusolin protein, entomopoxvirus)
[Em] Mês de entrada:1305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121212
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-012-0800-3


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[PMID]:20600091
[Au] Autor:Nalcacioglu R; Dizman YA; Vlak JM; Demirbag Z; van Oers MM
[Ad] Endereço:Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, Wageningen, The Netherlands.
[Ti] Título:Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025).
[So] Source:J Invertebr Pathol;105(3):363-5, 2010 Nov.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8h post inoculation (p.i.) and continued through 38hp.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase.
[Mh] Termos MeSH primário: DNA Viral/genética
Desoxirribodipirimidina Fotoliase/genética
Entomopoxvirinae/enzimologia
Mariposas/virologia
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Dano ao DNA/genética
Dano ao DNA/efeitos de radiação
Reparo do DNA/genética
DNA Viral/análise
Desoxirribodipirimidina Fotoliase/metabolismo
Entomopoxvirinae/genética
Dados de Sequência Molecular
Filogenia
Homologia de Sequência de Aminoácidos
Raios Ultravioleta
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins); EC 4.1.99.3 (Deoxyribodipyrimidine Photo-Lyase)
[Em] Mês de entrada:1102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101025
[St] Status:MEDLINE
[do] DOI:10.1016/j.jip.2010.06.013


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[PMID]:20447402
[Au] Autor:Takatsuka J; Okuno S; Ishii T; Nakai M; Kunimi Y
[Ad] Endereço:Department of Bioregulation and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan. junsan@ffpri.affrc.go.jp
[Ti] Título:Fitness-related traits of entomopoxviruses isolated from Adoxophyes honmai (Lepidoptera: Tortricidae) at three localities in Japan.
[So] Source:J Invertebr Pathol;105(2):121-31, 2010 Oct.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three entomopoxviruses (EPVs) isolated from diseased Adoxophyes honmai larvae at different localities (Tsukuba, Itsukaichi, and Miyazaki) in Japan were compared for biochemical identity and key parameters of virus fitness, fatal infection, speed of kill, and virus yield. When the structural peptides of occlusion bodies (OBs) and occlusion-derived viral particles were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, no difference in banding patterns was observed. However, DNA restriction endonuclease analysis showed that the three isolates were genotypically different, but many commonly sized DNA fragments were observed. Five tortricid species, A. honmai, Adoxophyes orana, Adoxophyesdubia, Homona magnanima, and Archips insulanus were susceptible to all isolates. No significant differences in the key viral fitness parameters were detected among the isolates in A. orana. However, the Miyazaki isolate had a different effect on H. magnanima; it allowed infected insects to survive longer and develop to a larger size, but had a lower yield of OBs per larva at any given time to death. OB yields per unit cadaver weight for the Miyazaki isolate, which indicate the conversion rate of the insect to virus, were lower over time compared to the other two isolates. The implications for selecting a candidate isolate to control tortricid pests are discussed.
[Mh] Termos MeSH primário: DNA Viral/análise
Entomopoxvirinae/genética
Aptidão Genética/fisiologia
Mariposas/virologia
Controle Biológico de Vetores
[Mh] Termos MeSH secundário: Animais
Entomopoxvirinae/patogenicidade
Entomopoxvirinae/fisiologia
Interações Hospedeiro-Patógeno
Japão
Controle Biológico de Vetores/métodos
Filogenia
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100831
[St] Status:MEDLINE
[do] DOI:10.1016/j.jip.2010.04.010


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[PMID]:20175214
[Au] Autor:Yu JF; Sun X
[Ad] Endereço:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
[Ti] Título:Reannotation of protein-coding genes based on an improved graphical representation of DNA sequence.
[So] Source:J Comput Chem;31(11):2126-35, 2010 Aug.
[Is] ISSN:1096-987X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Over annotation of protein coding genes is common phenomenon in microbial genomes, the genome of Amsacta moorei entomopoxvirus (AmEPV) is a typical case, because more than 63% of its annotated ORFs are hypothetical. In this article, we propose an improved graphical representation titled I-TN (improved curve based on trinucleotides) curve, which allows direct inspection of composition and distribution of codons and asymmetric gene structure. This improved graphical representation can also provide convenient tools for genome analysis. From this presentation, 18 variables are exploited as numerical descriptors to represent the specific features of protein coding genes quantitatively, with which we reannotate the protein coding genes in several viral genomes. Using the parameters trained on the experimentally validated genes, all of the 30 experimentally validated genes and 63 putative genes in AmEPV genome are recognized correctly as protein coding, the accuracies of the present method for self-test and cross-validation are 100%, respectively. Twenty-eight annotated hypothetical genes are predicted as noncoding, and then the number of reannotated protein coding genes in AmEPV should be 266 instead of 294 reported in the original annotations. Extending the present method trained in AmEPV to other entomopoxvirus genomes directly, such as Melanoplus sanguinipes entomopoxvirus (MsEPV), all of the 123 annotated function-known and putative genes are recognized correctly as protein coding, and 17 hypothetical genes are recognized as noncoding. The present method could also be extended to other genomes with or without adaptation of training sets with high accuracy.
[Mh] Termos MeSH primário: Biologia Computacional/métodos
Simulação por Computador
DNA Viral/química
DNA Viral/genética
Fases de Leitura Aberta/genética
Proteínas/genética
[Mh] Termos MeSH secundário: Algoritmos
Animais
Sequência de Bases
Gráficos por Computador
Entomopoxvirinae/genética
Genes Virais/genética
Genoma Viral/genética
Humanos
Homologia de Sequência de Aminoácidos
Especificidade da Espécie
Vírus Vaccinia/genética
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Proteins); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100614
[St] Status:MEDLINE
[do] DOI:10.1002/jcc.21500


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[PMID]:19477199
[Au] Autor:Perera SC; Wong P; Krell PJ; Arif BM
[Ad] Endereço:Laboratory for Molecular Virology, Great Lakes Forestry Centre, 1219 Queen St E, Sault Ste Marie, ON P6A 2E5, Canada. Anjali.Perera@NRCan-RNCan.gc.ca
[Ti] Título:Expression of heterologous genes in the Amsacta moorei entomopoxvirus.
[So] Source:J Virol Methods;165(1):1-8, 2010 Apr.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spheroidin (SPH) is the most abundant late protein in cells infected with the Amsacta moorei entomopoxvirus (AMEV). This locus can be used for expression of exogenous genes because it is not essential for virus replication. The sph promoter contains a conserved TAAATG motif, which serves as the site of initiation for both transcription and translation. Additional sequences downstream of the conserved motif have been shown to be involved in high-level expression of the sph gene. As a first step towards developing a protein expression vector based on the sph locus, four recombinant AMEV viruses expressing either gfp or lacZ were constructed. Both reporter genes were expressed under the control of the sph promoter containing the TAAATG motif. An additional 6 bp or 21 bp of sph coding region was included in three of the recombinants, to be expressed as an N-terminal fusion protein of GFP or LacZ. GFP and beta-galactosidase expression was observed at 2 days post-infection and continued throughout the observation period. The highest level of reporter gene expression was observed in the recombinant containing 21 bp from the sph coding region. These results indicate that sph locus of AMEV can be used successfully to express exogenous genes.
[Mh] Termos MeSH primário: Entomopoxvirinae/genética
Expressão Gênica
Engenharia Genética/métodos
Vetores Genéticos
Proteínas Recombinantes/biossíntese
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Genes Reporter
Proteínas de Fluorescência Verde/biossíntese
Proteínas de Fluorescência Verde/genética
Lepidópteros/virologia
Regiões Promotoras Genéticas
Proteínas Recombinantes de Fusão/biossíntese
Proteínas Recombinantes de Fusão/genética
Proteínas Estruturais Virais/genética
beta-Galactosidase/biossíntese
beta-Galactosidase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 0 (Viral Structural Proteins); 0 (spheroidin protein, Entomopoxvirus); 147336-22-9 (Green Fluorescent Proteins); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:1005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100315
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2009.05.015


  10 / 64 MEDLINE  
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[PMID]:20345294
[Au] Autor:Lawrence PO; Dillard BE
[Ad] Endereço:Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USA. pol@ifas.ufl.edu
[Ti] Título:A homolog of the vaccinia virus D13L rifampicin resistance gene is in the entomopoxvirus of the parasitic wasp, Diachasmimorpha longicaudata.
[So] Source:J Insect Sci;8:8, 2008.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The parasitic wasp, Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae), introduces an entomopoxvirus (DlEPV) into its Caribbean fruit fly host, Anastrepha suspensa. (Loew) (Diptera: Tephritidae), during oviposition. DlEPV has a 250-300 kb unipartite dsDNA genome, that replicates in the cytoplasm of the host's hemocytes, and inhibits the host's encapsulation response. The putative proteins encoded by several DlEPV genes are highly homologous with those of poxviruses, while others appear to be DlEPV specific. Here, a 2.34 kb sequence containing a 1.64 kb DlEPV open reading frame within a cloned 4.5 kb EcoR1 fragment (designated R1-1) is described from a DlEPV EcoRI genomic library. This open reading frame is a homolog of the vaccinia virus rifampicin resistance (rif) gene, D13L, and encodes a putative 546 amino acid protein. The DlEPV rif contains two EcoRV, two HindIII, one XbaI, and one DraII restriction sites, and upstream of the open reading frame the fragment also contains EcoRV, HindII, SpEI, and BsP106 sites. Early poxvirus transcription termination signals (TTTTTnT) occur 236 and 315 nucleotides upstream of the consensus poxvirus late translational start codon (TAAATG) and at 169 nucleotides downstream of the translational stop codon of the rif open reading frame. Southern blot hybridization of HindIII-, EcoRI-, and BamH1-restricted DlEPV genomic DNA probed with the labeled 4.5 kb insert confirmed the fidelity of the DNA and the expected number of fragments appropriate to the restriction endonucleases used. Pairwise comparisons between DlEPV amino acids and those of the Amsacta moorei, Heliothis armigera, and Melanoplus sanguinipes entomopoxviruses, revealed 46, 46, and 45 % similarity (identity + substitutions), respectively. Similar values (41-45%) were observed in comparisons with the chordopoxviruses. The mid portion of the DlEPV sequence contained two regions of highest conserved residues similar to those reported for H. armigera entomopoxvirus rifampicin resistance protein. Phylogenetic analysis of the amino acid sequences suggested that DlEPV arose from the same ancestral node as other entomopoxviruses but belongs to a separate clade from those of the grasshopper-infecting M. sanguinipes entomopoxvirus and from the Lepidoptera-infecting (Genus B or Betaentomopoxvirus) A. moorei entomopoxvirus and H. armigera entomopoxvirus. Interestingly, the DlEPV putative protein had only 3-26.4% similarity with RIF-like homologs/orthologs found in other large DNA non-poxviruses, demonstrating its closer relationship to the Poxviridae. DlEPV remains an unassigned member of the Entomopoxvirinae (http://www.ncbi.nlm.nih.gov/ICTVdb/Ictv/index.htm) until its relationship to other diptera-infecting (Gammaentomopoxvirus or Genus C) entomopoxviruses can be verified. The GenBank accession number for the nucleotide sequence data reported in this paper is EF541029.
[Mh] Termos MeSH primário: Farmacorresistência Viral/genética
Entomopoxvirinae/genética
Genes Virais/genética
Rifampina
Vírus Vaccinia/genética
Vespas/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antibióticos Antituberculose
Sequência de Bases
Dados de Sequência Molecular
Mapeamento por Restrição
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Homologia de Sequência do Ácido Nucleico
Proteínas Virais/química
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antibiotics, Antitubercular); 0 (Viral Proteins); VJT6J7R4TR (Rifampin)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100329
[St] Status:MEDLINE
[do] DOI:10.1673/031.008.0801



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