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Pesquisa : D01.625.100 [Categoria DeCS]
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Id: 12182
Autor: Bundy, Donald A. P; Foreman, J. D. M; Golden, Michael H. N.
Título: Sodium azide preservation of faecal specimens for Kato analysis
Fonte: Parasitology;90:463-9, 1985.
Idioma: En.
Resumo: The modified Kato technique has the advantages of reproducibility, simplicity and economy: the disadvantage is that it cannot be used in conjunction with traditional faecal preservatives. Sodium azide has been evaluated as a preservative for human faeces for subsequent Kato analysis. More than 400 faecal samples (from normal and malnourished children, and from mixed-age participants in a field survey of the Turks and Caicos Islands) were each mixed with 2-5mg of sodium azide powder and stored in 2 or 4ml autoanalyser cups at ambient tropical temperature. At intervals up to 30 weeks, aliquots were prepared for Kato analysis. Trichuria trichiura, Ascaris lumbricoides and Necator americanus eggs were well preserved without degenerative or developmental changes in morphology. Quantitative analyses of 18 samples indicated that the mean egg count/sample did not change significantly after storage for 1, 2, 4, 8, 12 and 16 weeks in preservative. The use of aside preservative extends the applications of the Kato technique to field and clinical studies in which delays may occur between specimen collection and examination. The direct costs of azide preservation are substantially lower than for traditional methods and the preserved specimens are more compact and resistant to leakage. (Summary)
Responsável: JM3.1 - Médical Library
JM9.1; QL757.A1P37

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Id: 6016
Autor: Foreman, J. D. M; Golden, Michael H. N; Bundy, Donald A. P.
Título: Faecal preservation with azide for diagnosis of helminthic infection by direct examination - abstract
Fonte: West Indian med. j;33(Suppl):49, 1984.
Idioma: En.
Conferência: Apresentado em: 29th Scientific Meeting of Commonwealth Caribbean Medical Research Council, Bridgetown, April 25-28, 1984.
Resumo: Direct examination of a cleared sample of faeces is a simpl, reliable and quantitative method for the diagnosis of helminthic ingection. As such, it is preferred to the cumbersome and less precise formalin preservation and concentration techniques. These latter method have had to be used in experiments or surveys where ther is a delay in analysis because established direct techniques required fresh, undiluted faeces. This study tested sodium azide as a preservative of faeces for later direct quantitative egg counts. Gresh faeces from chilren known to have Trichocephalus trichuris infection were each pressed through a 100-mesh sieve to remove fibre. Quadruplicate 50 mg aliquots of fresh stool were prepared and examined by the Kato thick-smear technique (Martin and Beaver, Amer J. Trop. Med. & Hyg., 17:382-391, 1968) and the eggs counted. 1.5 to 2g of faeces was stirred thoroughly using a match-stick with 6 mg of powdered sodium azide in a 4 ml autoanalyser cup. The container was capped and stored at ambient temperature (circa 25ºC). Egg counts were repeated at intervals. There was no evidence of fermentation of the stool. The mean egg counts of fresh stool and after preservation for 1, 2 and 4 week were 126 ± 45, 128 ± 44, 124 ± 46 and 137 ± 49. The standard deviation of the differences between the fresh samples and the preserved samples were 11, 10 and 16 respectively. None of these differences were significant. Stools from children containing T. trichuris, Necator americanus, and fertile and infertile Ascaris eggs were photographed whilst fresh and after azide preservation for up to 6 months. The morphology of the eggs appeared unchaged during this time. The azide preservation technique has made available direct quantitative analysis of stools in situations where stool must be preserved. It has been successfully used in a survey of the Turks and Caicos Islands where samples were transported to Jamaica for analysis (AU)
Responsável: JM3.1 - Médical Library
JM3.1; R18.W4

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