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Pesquisa : B01.050.150.900.649.801.400.112.199.120.510.550 [Categoria DeCS]
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  1 / 36008 MEDLINE  
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PMID:28091922
Autor:Chuguev YP; Chalyan VG; Meishvili NV; Chugueva II
Endereço:Institute of Medical Primatology, Sochi, Russia.
Título:Relationship between Serum Biochemistry in Macaca Mulatta Males and the Duration of Stay in Individual Cage.
Fonte:Bull Exp Biol Med; 162(3):391-394, 2017 Jan.
ISSN:1573-8221
País de publicação:United States
Idioma:eng
Resumo:A relationship between transfer of monkeys from yard to individual cage and duration of stay in individual cage and the serum biochemical parameters is detected in Macaca mulatta males.
Tipo de publicação: JOURNAL ARTICLE
Nome de substância:27YLU75U4W (Phosphorus); 9NEZ333N27 (Sodium); RWP5GA015D (Potassium); SY7Q814VUP (Calcium)


  2 / 36008 MEDLINE  
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PMID:27610576
Autor:Stauffer WR; Lak A; Yang A; Borel M; Paulsen O; Boyden ES; Schultz W
Endereço:Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK. Electronic address: wrs@pitt.edu.
Título:Dopamine Neuron-Specific Optogenetic Stimulation in Rhesus Macaques.
Fonte:Cell; 166(6):1564-1571.e6, 2016 Sep 08.
ISSN:1097-4172
País de publicação:United States
Idioma:eng
Resumo:Optogenetic studies in mice have revealed new relationships between well-defined neurons and brain functions. However, there are currently no means to achieve the same cell-type specificity in monkeys, which possess an expanded behavioral repertoire and closer anatomical homology to humans. Here, we present a resource for cell-type-specific channelrhodopsin expression in Rhesus monkeys and apply this technique to modulate dopamine activity and monkey choice behavior. These data show that two viral vectors label dopamine neurons with greater than 95% specificity. Infected neurons were activated by light pulses, indicating functional expression. The addition of optical stimulation to reward outcomes promoted the learning of reward-predicting stimuli at the neuronal and behavioral level. Together, these results demonstrate the feasibility of effective and selective stimulation of dopamine neurons in non-human primates and a resource that could be applied to other cell types in the monkey brain.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:9009-81-8 (Rhodopsin); VTD58H1Z2X (Dopamine)


  3 / 36008 MEDLINE  
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PMID:27529975
Autor:Karataev GI; Sinyashina LN; Medkova AY; Semin EG; Shevtsova ZV; Matua AZ; Kondzariya IG; Amichba AA; Kubrava DT; Mikvabia ZY
Título:[Insertional Inactivation of Virulence Operon in Population of Persistent Bordetella pertussis Bacteria].
Fonte:Genetika; 52(4):422-30, 2016 Apr.
ISSN:0016-6758
País de publicação:Russia (Federation)
Idioma:rus
Resumo:Avirulent B. pertussis bacteria containing IS elements in the bvgAS operon were detected during the study of whooping cough patients and bacilli carriers. The present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the B. pertussis bacteria in lower monkeys as the most adequate model for extrapolation ofthe experiment results to humans. By means of the real-time PCR method, it was established that the B. pertussis bacteria lived more than three months in the upper respiratory tract after a single intranasal monkey infection; the period was reduced to 14-28 days during repeated infection. An increase in the portion of B. pertussis Bvg mutants in the population to tens of percent from the total number of registered bacteria was registered. The experimental confirmation ofthe development and accumulation of avirulent B. pertussis Bvg mutants during the development of the infectious process was obtained. Further study of the composition of the B. pertussis persistent bacteria population at different stages of the disease will make it possible to formulate new approaches to the whooping cough diagnostics and prevention and creation of fundamentally new drugs.
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE
Nome de substância:0 (Bacterial Proteins); 0 (DNA Transposable Elements); 0 (Transcription Factors); 0 (bvgS protein, Bordetella pertussis)


  4 / 36008 MEDLINE  
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PMID:27409810
Autor:Bakken TE; Miller JA; Ding SL; Sunkin SM; Smith KA; Ng L; Szafer A; Dalley RA; Royall JJ; Lemon T; Shapouri S; Aiona K; Arnold J; Bennett JL; Bertagnolli D; Bickley K; Boe A; Brouner K; Butler S; Byrnes E; Caldejon S; Carey A; Cate S; Chapin M; Chen J; Dee N; Desta T; Dolbeare TA; Dotson N; Ebbert A; Fulfs E; Gee G; Gilbert TL; Goldy J; Gourley L; Gregor B; Gu G; Hall J; Haradon Z; Haynor DR; Hejazinia N; Hoerder-Suabedissen A; Howard R; Jochim J; Kinnunen M; Kriedberg A; Kuan CL; Lau C; Lee CK; Lee F
Endereço:Allen Institute for Brain Science, Seattle, Washington 98109, USA.
Título:A comprehensive transcriptional map of primate brain development.
Fonte:Nature; 535(7612):367-75, 2016 Jul 13.
ISSN:1476-4687
País de publicação:England
Idioma:eng
Resumo:The transcriptional underpinnings of brain development remain poorly understood, particularly in humans and closely related non-human primates. We describe a high-resolution transcriptional atlas of rhesus monkey (Macaca mulatta) brain development that combines dense temporal sampling of prenatal and postnatal periods with fine anatomical division of cortical and subcortical regions associated with human neuropsychiatric disease. Gene expression changes more rapidly before birth, both in progenitor cells and maturing neurons. Cortical layers and areas acquire adult-like molecular profiles surprisingly late in postnatal development. Disparate cell populations exhibit distinct developmental timing of gene expression, but also unexpected synchrony of processes underlying neural circuit construction including cell projection and adhesion. Candidate risk genes for neurodevelopmental disorders including primary microcephaly, autism spectrum disorder, intellectual disability, and schizophrenia show disease-specific spatiotemporal enrichment within developing neocortex. Human developmental expression trajectories are more similar to monkey than rodent, although approximately 9% of genes show human-specific regulation with evidence for prolonged maturation or neoteny compared to monkey.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL


  5 / 36008 MEDLINE  
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PMID:27376476
Autor:Katz LN; Yates JL; Pillow JW; Huk AC
Endereço:Center for Perceptual Systems, Departments of Neuroscience &Psychology, The University of Texas at Austin, Austin, Texas 78712, USA.
Título:Dissociated functional significance of decision-related activity in the primate dorsal stream.
Fonte:Nature; 535(7611):285-8, 2016 Jul 04.
ISSN:1476-4687
País de publicação:England
Idioma:eng
Resumo:During decision making, neurons in multiple brain regions exhibit responses that are correlated with decisions. However, it remains uncertain whether or not various forms of decision-related activity are causally related to decision making. Here we address this question by recording and reversibly inactivating the lateral intraparietal (LIP) and middle temporal (MT) areas of rhesus macaques performing a motion direction discrimination task. Neurons in area LIP exhibited firing rate patterns that directly resembled the evidence accumulation process posited to govern decision making, with strong correlations between their response fluctuations and the animal's choices. Neurons in area MT, in contrast, exhibited weak correlations between their response fluctuations and choices, and had firing rate patterns consistent with their sensory role in motion encoding. The behavioural impact of pharmacological inactivation of each area was inversely related to their degree of decision-related activity: while inactivation of neurons in MT profoundly impaired psychophysical performance, inactivation in LIP had no measurable impact on decision-making performance, despite having silenced the very clusters that exhibited strong decision-related activity. Although LIP inactivation did not impair psychophysical behaviour, it did influence spatial selection and oculomotor metrics in a free-choice control task. The absence of an effect on perceptual decision making was stable over trials and sessions and was robust to changes in stimulus type and task geometry, arguing against several forms of compensation. Thus, decision-related signals in LIP do not appear to be critical for computing perceptual decisions, and may instead reflect secondary processes. Our findings highlight a dissociation between decision correlation and causation, showing that strong neuron-decision correlations do not necessarily offer direct access to the neural computations underlying decisions.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T


  6 / 36008 MEDLINE  
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PMID:27279200
Autor:Behie AM; Groves CP
Endereço:The Australian National University, Canberra, Australia.
Título:China's primates: preserve wild species.
Fonte:Nature; 534(7606):179, 2016 Jun 08.
ISSN:1476-4687
País de publicação:England
Idioma:eng
Tipo de publicação: LETTER


  7 / 36008 MEDLINE  
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PMID:27236896
Autor:Zhu HB; Chen S; Chen YY; Lu FG; Ning XW
Título:[Effect of Chinese Herbal Extract HNA-1 on the Thymic Output Function in Simian Immunodeficiency Virus Chronically Infected Chinese Rhesus Macaques].
Fonte:Zhongguo Zhong Xi Yi Jie He Za Zhi; 36(3):351-8, 2016 Mar.
ISSN:1003-5370
País de publicação:China
Idioma:chi
Resumo:OBJECTIVE: To observe the effect of Chinese herbal extract HuNan A-1 (HNA-1) on the thymic output function in Simian immunodeficiency virus (SIV) chronically infected rhesus macaques. METHODS: Eight Chinese rhesus macaques had been infected by SIVmac239 for 16 to 21 months, and then they were randomly divided into the treatment group and the control group, 4 in each group. Monkeys in the treatment group were administered with HNA-1 by gastrogavage, once daily for 2 successive months, while those in the control group were administered with equal volume of normal saline by gastrogavage, once daily for 2 successive months. The general condition and body weight of monkeys were observed. Plasma viral loads were detected using real-time fluorescent quantitative PCR assay. CD4 percentages and counts, as well as naive CD subsets were detected using flow cytometry. T-cell receptor excision circles (TREC) were detected using real-time fluorescent quantitative PCR assay. The thymus tissue was pathologically observed using routine HE staining. The correlation between lesions of the thymus tissue, CD4 counts, naive CD counts, and TREC were analyzed. RESULTS: There was no statistical difference in body weight, viral loads, absolute CD ratios between the two groups after treatment (P > 0.05). The altered TREC multiple showed an obvious decreasing tendency in the control group, while it showed an increasing tendency in the treatment group (P < 0.05). In both groups, destroyed structures of the thymus tissue could be seen, filled with pink unstructured material. Increased connective tissues, lowered connective cell density, and confused arrangement could also be seen in the two groups, with no obvious difference. TREC contents were positively correlated with naive CD4 counts after removing extremum (r = 0.926, P = 0.001). Naive CD4 counts were positively correlated with CD4 counts (r = 0.961, P = 0.005). CONCLUSIONS: TREC content determination, as a marker of newly thymic emigrants, could be taken as a testing method for evaluating the thymic output function. Besides, HNA-1 treatment increased the thymic output significantly in SIV chronically infected monkeys. Correlation existed among TREC contents, naive CD4 counts, and pathologies of thymus tissues, especially in late infection stage.
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE
Nome de substância:0 (Drugs, Chinese Herbal); 0 (Plant Extracts)


  8 / 36008 MEDLINE  
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PMID:27160350
Autor:Pardee K; Green AA; Takahashi MK; Braff D; Lambert G; Lee JW; Ferrante T; Ma D; Donghia N; Fan M; Daringer NM; Bosch I; Dudley DM; O'Connor DH; Gehrke L; Collins JJ
Endereço:Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.
Título:Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Fonte:Cell; 165(5):1255-66, 2016 May 19.
ISSN:1097-4172
País de publicação:United States
Idioma:eng
Resumo:The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises. PAPERCLIP.
Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de substância:0 (RNA, Viral)


  9 / 36008 MEDLINE  
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PMID:27151032
Autor:Ma YC; Li M; Xing S; Xiong GL; Shen X; Chen Q; Cong YW; Wang JX; Zhu NK; Yu ZY; Zhang XG
Endereço:Medical College of Soochow University, Suzhou 215006, Jiangsu Province, China.
Título:[Therapeutic Effect of Combined Cytokines on Nonhuman Primate Model of Severe Haemopoietic Acute Radiation Sickness].
Fonte:Zhongguo Shi Yan Xue Ye Xue Za Zhi; 24(2):573-9, 2016 Apr.
ISSN:1009-2137
País de publicação:China
Idioma:chi
Resumo:OBJECTIVE: To evaluate the therapeutic effects of combined administration of recombinant human granulocyte colony-stimulating factor (rhG-CSF), recombinant human thrombopoietin (rhTPO) and recombinant human interleukin-2 (rhIL-2) on radiation-induced severe haemopoietic acute radiation sickness (ARS) in rhesus monkeys, so as to provide experimental evidences for the effective clinical treatment. METHODS: Seventeen rhesus monkeys were exposed to 7.0 Gy (60)Co γ-ray total body irradiation (TBI) to establish severe haemopoietic ARS model, and were randomly divided into supportive care group, rhG-CSF+rhTPO treatment group and rhG-CSF+rhTPO+rhIL-2 treatment group. Survival time, general signs such as bleeding and infections, and peripheral blood cell counts in each group were monitored. Bone marrow cells were cultivated to examine the colony formation ability. The histomorphology changes of bone marrow were observed at 45 d post irradiation. RESULTS: After 7.0 Gy (60)Co γ-ray TBI, monkeys of supportive care group underwent tarry stool and emesis, then died in 12~18 d. The overall survival rate in this group was 16.7%. Gastrointestinal reactions of monkeys in two combined-cytokines treatment groups were inapparent. Combined-cytokines treatment induced 100% survival. Complete blood cells declined sharply after irradiation in each group, but two combined-cytokines treatment schemes could elevate the nadir of all blood cells, shorten the duration of pancytopenia and accelerate the recovery of hemogram. Compared with rhG-CSF+ rhTPO treatment, rhG-CSF+ rhTPO+ rhIL-2 treatment could increase the counts of lymphocytes and monocytes. The colony-formation rate of haemopoietic stem/progenitor cells in bone marrow dropped markedly at 2 d after irradiation. Combined-cytokines treatment promoted the ability of colony formation on day 29. Hematopoietic cells mostly disappeared in bone marrow of animals in supportive care group, but hematopoietic functions were recovered after cytokines were administrated. CONCLUSION: rhG-CSF+ rhTPO and rhG-CSF+ rhTPO+ rhIL-2 treatment can significantly promote hematopoiesis recovery, improve the quantity of life, simplify the supportive therapy, and enhance the survival rate of rhesus monkeys with severe haemopoietic ARS induced by 7.0 Gy (60)Co γ-ray exposure. Especially the application of rhIL-2 can accelerate the recovery of lymphocytes and monocytes and restore the immunological function. Thus, combination of rhG-CSF, rhTPO and rhIL-2 on the basis of supportive care is an efficient strategy to treat severe haemopoietic ARS.
Tipo de publicação: ENGLISH ABSTRACT; JOURNAL ARTICLE
Nome de substância:0 (Interleukin-2); 0 (Recombinant Proteins); 143011-72-7 (Granulocyte Colony-Stimulating Factor); 9014-42-0 (Thrombopoietin)


  10 / 36008 MEDLINE  
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PMID:27120866
Título:NIH to End Research Targeted by PETA.
Fonte:Physiologist; 59(2):103-4, 2016 Mar.
ISSN:0031-9376
País de publicação:United States
Idioma:eng
Tipo de publicação: JOURNAL ARTICLE



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