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Id: 703202
Autor: Zambonato, Bianca P; Daemon, Erik; Prezoto, Fábio.
Título: An alternative technique for individual marking of orb-web spiders / Uma técnica alternativa para a marcação individual de aranhas orbitelas
Fonte: Rev. etol. (Online);9(1):3-5, jan. 2010. ilus.
Idioma: en.
Resumo: A technique is proposed for individually marking orb-web spiders aiming at facilitating the identification of the individuals on their webs. To check the efficiency of the method, 17 females and 30 males of Nephila clavipes (Linnaeus 1767) were marked on the dorsal part of the opisthosoma using enamel applied through a disposable syringe. Success was obtained in marking 47 individuals; no death, dispersal or agonistic behavior was observed after the use of the method. This marking did not dissolve in contact with water, resisted the expansion of the opisthosoma and was lost only in the ecdysis. This method also was efficient for males that, being smaller than the females, demand a more careful methodology(AU)

Propõe-se uma técnica de marcação individual de aranhas orbitelas visando facilitar a identificação dos indivíduos em suas teias. Para verificar a eficiência do método, 17 fêmeas e 30 machos de Nephila clavipes (Linnaeus, 1767) foram marcados na parte dorsal do abdômen utilizando-se esmalte, aplicado por meio de seringa descartável. Obteve-se sucesso na marcação dos 47 indivíduos, não sendo observada morte, dispersão ou comportamentos agonísticos após o uso do método. Esta marcação não dissolveu em contato com a água, resistiu ao aumento do opistossoma e foi perdida somente na ecdise. Esse método também foi eficiente para os machos que, por serem menores que as fêmeas, exigem uma metodologia mais cuidadosa.(AU)
Descritores: Aranhas
Técnicas
Diferenciação Sexual
Limites: Animais
Masculino
Feminino
Responsável: BR85.1 - Biblioteca Dante Moreira Leite


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Id: 700304
Autor: França, Renné Oliveira.
Título: Memória eletrônica: a mnemotécnica da retrospectiva de final de ano / Electronic memory: the mnemotechnique from the retrospective of the end year
Fonte: Ciênc. cogn;16(1):75-98, 2011.
Idioma: pt.
Resumo: A partir da discussão das relações entre a televisão e a memória coletiva, este trabalho descreve e analisa o programa Retrospectiva de Fim de Ano, veiculado pela Rede Globo de Televisão. O artigo procura caracterizar o programa como uma peculiar mnemotécnica que, através de recursos expressivos próprios do meio eletrônico, reescreve os acontecimentos midiáticos, destacando o seu lugar na experiência social e tornando-os memoráveis. Para tal, foram observados dez programas entre os anos 1996 e 2005. Não se pretende, aqui, definir uma mnemotécnica própria da mídia ou, mais especificamente, da televisão. O objetivo principal é analisar a técnica de memória de um programa específico, procurando demonstrar de que maneira ele constitui uma memória eletrônica, de natureza partilhada por seus telespectadores. O programa apresenta os acontecimentos midiáticos que já apareceram durante o ano e, através de recursos narrativos próprios da televisão, oferece-os novamente para seus telespectadores. Seguindo o caminho traçado pelo programa Retrospectiva, buscamos conhecer sua peculiar técnica de memória eletrônica e coletiva.(AU)
Descritores: Memória
-Técnicas
Narração
Responsável: BR2474.1 - Biblioteca


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Id: 678984
Autor: Court P., Ana K; Martínez Gómez, Juan Carlos.
Título: Técnicas de localización radiográfica en endodoncia: revisión bibliográfica / Radiographic techniques in endodontics location: literature review
Fonte: Acta odontol. venez;50(4), 2012. ilus.
Idioma: es.
Resumo: La radiología representa un área muy importante dentro del campo médico y odontológico, siendo básica y esencial para el diagnóstico y, por lo tanto, para la terapéutica. En endodoncia, la toma de radiografías es imprescindible durante las diferentes etapas del tratamiento, para poder culminar con éxito la terapia endodóntica, por lo que es fundamental la obtención de radiografías de alta calidad diagnóstica. En determinadas situaciones, aún cuando la técnica de toma de la radiografía y su proceso de revelado hayan permitido la obtención de radiografías con imágenes adecuadas, las mismas no son útiles para el propósito que se requieren, como puede ser la localización de un objeto extraño, la ubicación de una estructura anatómica que imposibilita la visión directa a una pieza dentaria o de un conducto radicular en específico, por lo que el odontólogo debe manejar las diferentes técnicas de localización radiográfica y su interpretación. En la presente revisión se destaca la importancia de las técnicas de localización radiográfica, su definición, indicaciones y técnicas de localización aplicadas al área de endodoncia, los aspectos básicos para su realización y como se interpretan las imágenes obtenidas(AU)

The Radiology represents an important area in the medical and dentist field, it's basic and essential for the diagnostic and therapeutic. The endodontic treatment demands the use of radiographies during it's different stages, this radiographies must be of the must high quality to finish successfully the dentist therapy. In certain occasions, the radiographies don't attain to get the result for it has been used, even when the Dentist had used the correctly radiographic technology and correct radiographic film processing, because it doesn't easy show the location of rare objects, anatomic structures, tooth or root canals. The present review make emphasize in the importance of location radiographic technics, it's indications, definitions and locations technics applied to the endodontic treatment, it's basics aspects of carrying out and how to interpret the x-ray image(AU)
Descritores: Radiografia Dentária
Métodos
Técnicas
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: 676919
Autor: Silva, Ronivaldo Rodrigues da; Cabral, Tatiana Pereira de Freitas; Rodrigues, André; Hamilton, Cabral.
Título: Production and partial characterization of serine and metallo peptidases secreted by Aspergillus fumigatus Fresenius in submerged and solid state fermentation
Fonte: Braz. j. microbiol;44(1):235-243, 2013. ilus, tab.
Idioma: en.
Resumo: Enzyme production varies in different fermentation systems. Enzyme expression in different fermentation systems yields important information for improving our understanding of enzymatic production induction. Comparative studies between solid-state fermentation (SSF) using agro-industrial waste wheat bran and submerged fermentation (SmF) using synthetic media were carried out to determinate the best parameters for peptidase production by the fungus Aspergillus fumigatus Fresen. Variables tested include: the concentration of carbon and protein nitrogen sources, the size of the inoculum, the pH of the media, temperature, and the length of the fermentation process. The best peptidase production during SSF was obtained after 96 hours using wheat bran at 30 ºC with an inoculum of 1 x 10(6) spores and yielded 1500 active units (UµmL). The best peptidase production using SmF was obtained after periods of 72 and 96 hours of fermentation in media containing 0.5% and 0.25% of casein, respectively, at a pH of 6.0 and at 30 ºC and yielded 40 UµmL. We also found examples of catabolite repression of peptidase production under SmF conditions. Biochemical characterization of the peptidases produced by both fermentative processes showed optimum activity at pH 8.0 and 50 ºC, and also showed that their proteolytic activity is modulated by surfactants. The enzymatic inhibition profile using phenylmethylsulfonyl fluoride (PMSF) in SmF and SSF indicated that both fermentative processes produced a serine peptidase. Additionally, the inhibitory effect of the ethylene-diaminetetraacetic acid (EDTA) chelating agent on the peptidase produced by SmF indicated that this fermentative process also produced a metallopeptidase.(AU)
Descritores: Aspergillus fumigatus/enzimologia
Aspergillus fumigatus/isolamento & purificação
Fermentação
Metaloexopeptidases/análise
Metaloexopeptidases/isolamento & purificação
Peptídeo Hidrolases/análise
Serina/análise
Azotobacter/enzimologia
Azotobacter/isolamento & purificação
-Parâmetros
Métodos
Ativação Enzimática
Técnicas
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica


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Id: 676918
Autor: Belal, Elsayed B.
Título: Bioethanol production from rice straw residues
Fonte: Braz. j. microbiol;44(1):225-234, 2013. ilus, tab.
Idioma: en.
Resumo: A rice straw -cellulose utilizing mold was isolated from rotted rice straw residues. The efficient rice straw degrading microorganism was identified as Trichoderma reesei. The results showed that different carbon sources in liquid culture such as rice straw, carboxymethyl cellulose, filter paper, sugar cane bagasse, cotton stalk and banana stalk induced T. reesei cellulase production whereas glucose or Potato Dextrose repressed the synthesis of cellulase. T. reesei cellulase was produced by the solid state culture on rice straw medium. The optimal pH and temperature for T. reesei cellulase production were 6 and 25 ºC, respectively. Rice straw exhibited different susceptibilities towards cellulase to their conversion to reducing sugars. The present study showed also that, the general trend of rice straw bioconversion with cellulase was more than the general trend by T. reesei. This enzyme effectively led to enzymatic conversion of acid, alkali and ultrasonic pretreated cellulose from rice straw into glucose, followed by fermentation into ethanol. The combined method of acid pretreatment with ultrasound and subsequent enzyme treatment resulted the highest conversion of lignocellulose in rice straw to sugar and consequently, highest ethanol concentration after 7 days fermentation with S. cerevisae yeast. The ethanol yield in this study was about 10 and 11 g.L-¹.(AU)
Descritores: Etanol/análise
Trichoderma/enzimologia
Trichoderma/isolamento & purificação
Celulase/análise
Celulase/isolamento & purificação
Oryza sativa/enzimologia
Carbono
Resíduos Orgânicos
Biomassa
Microbiologia Industrial
-Hidrólise
Métodos
Técnicas
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica


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Id: 676917
Autor: Lin, Qian.
Título: Submerged fermentation of Lactobacillus rhamnosus YS9 for γ aminobutyric acid (GABA) production
Fonte: Braz. j. microbiol;44(1):183-187, 2013. ilus.
Idioma: en.
Projeto: Research Foundation for Advanced Talents of Yulin Normal University; . Guangxi Natural Science Foundation; . Guangxi Feature Specialty and Curriculum Integration Project. G2009042; 2011GXNSFB018046; GXTSZY220; GXTSZY022.
Resumo: γ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter in central nervous system, and its application in drugs and functional foods has attracted great attention. To enhance production of y-aminobutyric acid, Lactobacillus rhamnosus YS9, a strain isolated from Chinese traditional fermented food pickled vegetable, was grown under submerged fermentation. Its cultivation conditions were investigated. When culture pH condition was adjusted to the optimal pH of glutamate decarboxylase activity, culture of Lb. rhamnosus YS9 in medium supplemented with 200 mM of monosodium glutamate and 200 µM of pyridoxal phosphate (PLP), produced 187 mM of GABA.(AU)
Descritores: Fermentação
Lactobacillus rhamnosus/isolamento & purificação
Lactobacillus rhamnosus/enzimologia
Glutamato Descarboxilase/análise
Glutamato Descarboxilase/isolamento & purificação
Microbiologia Industrial
-Métodos
Técnicas
Ativação Enzimática
Amostras de Alimentos
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica


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Avila, Fernando Antonio de
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Id: 676916
Autor: Rigobelo, Everlon Cid; Blackall, Patrick Joseph; Maluta, Renato Pariz; Ávila, Fernando Antonio de.
Título: Identification and antimicrobial susceptibility patterns of Pasteurella multocida isolated from chickens and japanese quails in Brazil
Fonte: Braz. j. microbiol;44(1):161-164, 2013. tab.
Idioma: en.
Resumo: A study was performed to verify the presence of Pasteurella multocida in eight different poultry groups of 90 birds each. Groups I to IV were chickens (I being > 6 weeks of age with a history of respiratory illness, II > 6 weeks of age and free of respiratory illness, III < 6 weeks of age with respiratory illness and IV being < 6 weeks of age and with no respiratory illness. Groups V to VIII had the matching characteristics of Groups I to V but consisted of Japanese Quails. The P. multocida isolation rate from the groups was as follows; Group I 56/90 (62.3%) Group II 18/90 (20.0%), Group III 12/90 (13.3%), Group IV 3/90 (3.33%), Group V 8/90 (8.88%), Group VI 2/90 (2.22%) Group VII 2/90 (2.22%) and Group VIII 1/90 (1.11%). These isolation rates were not significantly different within the groups of a bird type but the overall chicken isolation rate was significantly higher than the quail isolation rate (p < 0.01). All isolates were examined for their sensitivity to four antimicrobial agents. The results showed only low levels of resistance to the agents tested. The highest level of resistance detected was to cephalothin (5.1% of isolates) followed by amikacin (3.4%).(AU)
Descritores: Infecções por Pasteurella
Pasteurella multocida/isolamento & purificação
Doenças Respiratórias
Aves Domésticas
Cefalotina/isolamento & purificação
Resistência Microbiana a Medicamentos
Suscetibilidade a Doenças
-Galinhas
Coturnix
Métodos
Técnicas
Limites: Animais
Tipo de Publ: Estudo Comparativo
In Vitro
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica


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Alves, Lucimara Aparecida
experimentalDocumentos relacionados
Id: 676910
Autor: Gonçalves, Daniela Dib; Benitez, Aline; Lopes-Mori, Fabiana Maria Ruiz; Alves, Lucimara Aparecida; Freire, Roberta Lemos; Navarro, Italmar Teodorico; Santana, Maria Aparecida Zanella; Santos, Luís Roberto Alves dos; Carreira, Teresa; Vieira, Maria Luísa; Freitas, Julio Cesar de.
Título: Zoonoses in humans from small rural properties in Jataizinho, Parana, Brazil
Fonte: Braz. j. microbiol;44(1):125-131, 2013. ilus, tab.
Idioma: en.
Resumo: The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural area inhabitants were tested for indirect immunofluorescence (IFI) and western blots (WB) were performed to detect Borrelia burgdorferi (sensu lato); a tamponated acidified antigen test (AAT) and 2-mercaptoethanol (2-ME) were used to detect antibodies of Brucella abortus; the microscopic agglutination test (MAT) was carried out to detect antibodies anti-Leptospira spp. and IFI was used to find antibodies of Toxoplasma gondii. Two of the samples (0.96%) were reactive for Lyme borreliosis, three (1.4%) for brucellosis, 25 (12.1%) for leptospirosis and 143 (69.1%) for toxoplasmosis. Although the town of Jataizinho has a human development index (IDH) that was considered to be average (0.733) in the state of Parana, the low social, economic and cultural conditions of the population from small rural properties have resulted in lack of basic information on animal health and direct or indirect contact with the various species of domestic animals, wildlife and ticks have probably contributed to the prevalence levels found. These results show the need for additional regional studies in order to determine the epidemiological characteristics of these diseases as well as their respective vectors and reservoirs so that effective prophylaxis can be administered in the human population.(AU)
Descritores: Doença de Lyme
Brucelose
Leptospirose
Anticorpos
Sorologia
-Imunofluorescência
Zoonoses
Métodos
Técnicas
Aglutinação
Limites: Humanos
Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica


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Lage, A. P
experimentalDocumentos relacionados
Id: 676900
Autor: Viott, A. M; Lage, A. P; Cruz Junior, E. C. C; Guedes, R. M. C.
Título: The prevalence of swine enteropathogens in Brazilian grower and finish herds
Fonte: Braz. j. microbiol;44(1):145-151, 2013. tab.
Idioma: en.
Resumo: Diarrhoea among growing and finishing pigs is an important problem in many herds. The prevalence of L. intracellularis, B. pilosicoli, B. hyodysenteriae, Salmonella spp., enterotoxigenic E. coli, Trichuris suis and the occurrence of mixed infection were investigated. Fecal samples for forty-six herds with diarrhea or a history of diarrhea were randomly collected in Minas Gerais state, Brazil. The enteric pathogens were detected by culture (E. coli and Salmonella sp.), PCR (L. intracellularis and Brachyspira spp.) and eggs counts (T. suis). The overall herd prevalence of L. intracellularis, Salmonella enterica serotype Typhimurium and enterotoxigenic E. coli were 19.56%, 6.52%, 10.86% respectively. Mixed infection was diagnosed in 30.43% of herds, and L. intracellularis and Salmonella enterica serotype Typhimurium are main pathogens association (10.87%). B. pilosicoli was diagnosed only in two herds, always associated with mixed infections. B. hyodysenteriae and T. suis were not demonstrated in any sample. These pathogens have been reported world-wide but studies regarding epidemiology in Brazil are few. This study contributes to establish of prevention programs for the control enteropathogens in grower finish herds in Brazil.(AU)
Descritores: Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/patogenicidade
Infecções por Enterobacteriaceae/diagnóstico
Suínos
Disenteria Bacilar/diagnóstico
Técnicas e Procedimentos Diagnósticos
-Epidemiologia
Virulência
Métodos
Técnicas
Diagnóstico
Limites: Animais
Tipo de Publ: In Vitro
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica


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Id: 676896
Autor: Zárate-Chaves, Carlos Andrés; Romero-Rodríguez, María Camila; Niño-Arias, Fabián Camilo; Robles-Camargo, Jorge; Linares-Linares, Melva; Rodríguez-Bocanegra, María Ximena; Gutiérrez-Rojas, Ivonne.
Título: Optimizing a culture medium for biomass and phenolic compounds production using Ganoderma lucidum
Fonte: Braz. j. microbiol;44(1):215-223, 2013. ilus, tab.
Idioma: en.
Projeto: Universidad Javeriana. ID project 002952.
Resumo: The present work was aimed at optimizing a culture medium for biomass production and phenolic compounds by using Ganoderma lucidum. The culture was optimized in two stages; a Plackett-Burman design was used in the first one for identifying key components in the medium and a central composite design was used in the second one for optimizing their concentration. Both responses (biomass and phenolic compounds) were simultaneously optimized by the latter methodology regarding desirability, and the optimal concentrations obtained were 50.00 g/L sucrose, 13.29 g/L yeast extract and 2.99 g/L olive oil. Maximum biomass production identified in these optimal conditions was 9.5 g/L and that for phenolic compounds was 0.0452 g/L, this being 100% better than that obtained in the media usually used in the laboratory. Similar patterns regarding chemical characterization and biological activity towards Aspergillus sp., from both fruiting body and mycelium-derived secondary metabolites and extracts obtained in the proposed medium were observed. It was shown that such statistical methodologies are useful for optimizing fermentation and, in the specific case of G. lucidum, optimizing processes for its production and its metabolites in submerged culture as an alternative to traditional culture.(AU)
Descritores: Compostos Fenólicos/análise
Reishi/isolamento & purificação
Biomassa
Meios de Cultura/análise
Micélio/isolamento & purificação
-Metodologia
Técnicas
Otimização
Tipo de Publ: Estudos de Avaliação
Responsável: BR32.1 - SBIB - Serviço de Biblioteca e Informação Biomédica



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