Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris
Genet. mol. biol
; 34(3): 464-470, 2011. ilus
Artigo
em Inglês
| LILACS
| ID: lil-595977
Biblioteca responsável:
BR1.1
ABSTRACT
The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production. The entire recombinant human erythropoietin (rhEPO) gene was constructed using the Splicing by Overlap Extension by PCR (SOE-PCR) technique, cloned and expressed through the secretory pathway of the Pichia expression system. Recombinant erythropoietin was successfully expressed in P. pastoris. The estimated molecular mass of the expressed protein ranged from 32 kDa to 75 kDa, with the variation in size being attributed to the presence of rhEPO glycosylation analogs. A crude functional analysis of the soluble proteins showed that all of the forms were active in vivo.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Pichia
/
Glicosilação
/
Eritropoetina
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Malásia
Instituição/País de afiliação:
Institute of Biological Sciencsa/MY
/
University of Malaya/MY
