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Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells
Tayarani-Najarani, Zahra; Asili, Javad; Parsaee, Heydar; Mousavi, Seyed Hadi; Mashhadian, Naser Vadati; Mirzaee, Alireza; Emami, Seyed Ahmad.
Afiliação
  • Tayarani-Najarani, Zahra; University of Medical Sciences, Mashhad. School of Medicine, Mashhad. Pharmacological Research Centre of Medicinal Plants. IR
  • Asili, Javad; University of Medical Sciences, Mashhad. School of Pharmacy, Mashhad. Department of Pharmacognosy. IR
  • Parsaee, Heydar; University of Medical Sciences, Mashhad. School of Medicine, Mashhad. Pharmacological Research Centre of Medicinal Plants. IR
  • Mousavi, Seyed Hadi; University of Medical Sciences, Mashhad. School of Medicine, Mashhad. Pharmacological Research Centre of Medicinal Plants. IR
  • Mashhadian, Naser Vadati; University of Medical Sciences, Mashhad. Medical Toxicology Research Center. IR
  • Mirzaee, Alireza; University of Medical Sciences, Mashhad. School of Pharmacy, Mashhad. Department of Pharmacognosy. IR
  • Emami, Seyed Ahmad; University of Medical Sciences, Mashhad. School of Pharmacy, Mashhad. Department of Pharmacognosy. IR
Rev. bras. farmacogn ; 22(2): 268-276, Mar.-Apr. 2012. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-624645
Biblioteca responsável: BR1.1
ABSTRACT
Chemical investigation on the CH2Cl2 fraction of the Scutellaria litwinowii Bornm. & Sint., Lamiaceace, root extract for the first time resulted in the isolation of wogonin, and neobaicalein. These compounds were evaluated for their cytotoxicity towards HeLa cell lines and lymphocytes. Meanwhile, the role of apoptosis was explored in this toxicity. The cells were cultured in RPMI medium and incubated with different concentrations of isolated flavonoids. Cell viability was quantified by MTS assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1peak). Wogonin, and neobaicalein inhibited the growth of malignant cells in a dose-dependent manner. The IC50 values of 46.62 and 79.34 µM were, respectively, found for neobaicalein and wogonin against HeLa cells after 48 h of treatment. Neobaicalein induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in neobaicalein toxicity. Neobaicalein exerts cytotoxic and pro-apoptotic effects in HeLa cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Rev. bras. farmacogn Assunto da revista: Farmácia Ano de publicação: 2012 Tipo de documento: Artigo País de afiliação: Irã Instituição/País de afiliação: University of Medical Sciences, Mashhad/IR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Idioma: Inglês Revista: Rev. bras. farmacogn Assunto da revista: Farmácia Ano de publicação: 2012 Tipo de documento: Artigo País de afiliação: Irã Instituição/País de afiliação: University of Medical Sciences, Mashhad/IR
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