Development of transformation vectors for the production of potentially high oleate transgenic oil palm
Electron. j. biotechnol
; 11(3): 23-31, July 2008. ilus
Artigo
em Inglês
| LILACS
| ID: lil-531896
Biblioteca responsável:
CL1.1
ABSTRACT
The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense beta-ketoacyl-ACP-synthase II (KASII) and sense delta-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Óleo de Palmeira
/
Plantas Geneticamente Modificadas
/
Vetores Genéticos
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Malásia
Instituição/País de afiliação:
Malaysian Palm Oil Board/MY
